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All Journal Chemistry Indonesian Journal of Chemical Research Jambura Journal of Educational Chemistry Jambura Journal of Chemistry Jurna Beta Kimia Damhil: Jurnal Pengabdian kepada Masyarakat
Musa, Weny J.A.
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Other

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Isolasi Senyawa Metabolit Sekunder dan Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Bidara (Ziziphus mauritiana) Bialangi, Nurhayati; Sahami, Ulin; Musa, Weny J.A.; Kunusa, Wiwin Rewini; Aman, La Ode
Jambura Journal of Chemistry Vol 5, No 1 (2023): February
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jambchem.v5i1.16022

Abstract

Penelitian ini bertujuan mengisolasi dan mengidentifikasi senyawa metabolit sekunder serta mengetahui aktivitas antioksidan yang terkandung dalam ekstrak etil asetat daun Z. mauritiana. Tahap isolasi diawali dengan ekstraksi secara maserasi menggunakan pelarut etil asetat. Uji fitokimia menunjukkan ekstrak etil asetat positif mengandung flavonoid, tanin dan terpenoid. Proses pemisahan dilakukan menggunakan kromatografi lapis tipis dengan campuran eluen n-heksana : etil asetat (9:1 dan 8:2) dilanjutkan dengan kromatografi kolom. Fraksi-fraksi yang diperoleh dilakukan proses pemurnian menggunakan metode rekristalisasi. Isolat nomor 14 diduga murni berupa kristal jarum berwarna hijau kekuningan sebanyak 4,5 mg. Uji kemurnian menggunakan KLT dua dimensi dengan eluen pertama n-heksana : etil asetat (8:2) dan eluen kedua kloroform : metanol (9:1) diperoleh satu bercak noda tunggal. Spektrum UV-Vis menunjukkan adanya gugus kromofor tak jenuh C=C (251 nm) dan kromofor C=O (283 nm). Sedangkan, spektrum IR menunjukkan adanya gugus ulur OH gugus, ulur C-H alifatik, gugus tekuk C-H, gugus fungsi C=O, C=C dan gugus fungsi ulur C-O. Hasil uji aktivitas antioksidan menunjukkan bahwa kemampuan reduktif dari ekstrak daun Z. mauritiana yang berbeda secara signifikan jika dibandingkan dengan standar vitamin C. Ekstrak daun Z. mauritiana memiliki aktivitas lebih rendah (IC50 489.9936 "μg/ml" ) dibandingkan dengan standar vitamin C.
Triterpene Compound from Ethyl Acetate Fraction of Kesambi Bark (Schleichera oleosa) and Its Activity as Anti-Bacterial Musa, Weny J.A.; Setiyandani, Kurnia Indah; Mustafid, Afif Hidayatul; Bialangi, Nurhayati; Situmeang, Boima
Indonesian Journal of Chemical Research Vol 11 No 3 (2024): Edition for January 2024
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2024.11-wen

Abstract

In our earlier study, we managed to find the antibacterial activity of the ethyl acetate fraction of the kesambi bark stem. In our next study, we isolated secondary metabolite compounds from the ethyl acetate fraction of the kesambi stem bark and tested the antibacterial activity of isolates against the bacteria Escherichia coli and Staphylococcus aureus. Separation is carried out by the method of column chromatography. Analysis of stain patterns and purity was performed with thin-layer chromatography (TLC). The test of antibacterial activity was carried out by the Kirby Bauer method. Characterization of pure isolates was performed with IR, 1H -NMR, 13C -NMR and DEPT 135° spectroscopy. The results of the characterization of pure isolates show that the isolated compound is a triterpenoid compound with the name IUPAC compound lup-20(29)-en-3-ol. The results of antibacterial tests showed that the compound lup-20(29)-en-3-ol has antibacterial activity against E. coli and S. aureus which is relatively moderate.
Pendayagunaan Limbah Gula Aren di Desa Mongiilo sebagai Sumber Pupuk Organik Ramah Lingkungan Musa, Weny J.A.; Duengo, Suleman; Arviani, Arviani; Kilo, Ahmad Kadir
Damhil: Jurnal Pengabdian kepada Masyarakat Vol 4, No 1: June 2025
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/damhil.v4i1.32849

Abstract

This community service program focuses on utilizing domestic waste from the sugar palm craftsmen in Desa Mongiilo to create organic fertilizer. The project aimed to address the underutilization of organic waste from sugar palm production by converting it into a valuable agricultural input. The initiative involved training local farmers in organic farming methods, including the production of organic fertilizers using sugar palm waste. The results of this program showed significant improvements in soil fertility and plant health, thereby enhancing agricultural productivity in the village. This program has the potential to be a sustainable solution for waste management and agricultural enhancement in rural areas.
Microemulsion of methanol extract of Tridax procumbens flower and its antibacterial activity against Streptococcus mutans and Enterococcus faecalis Wijayanti, Sri; Pradana, Agrin Febrian; Situmeang, Boima; Prastiwi, Dina Alva; Musa, Weny J.A.
Jurnal Beta Kimia Vol 5 No 1 (2025): Volume 5 Number 1, May 2025
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jbk.v5i1.21189

Abstract

Tridax procumbens (commonly known as Gletang flower) is a medicinal plant recognized for its antibacterial potential and is widely distributed across various habitats such as rice fields, plantations, and roadsides. Phytochemical screening of its methanolic flower extract revealed the presence of secondary metabolites, including alkaloids, flavonoids, steroids, phenols, terpenoids, and tannins, all of which contribute to its biological activities. This study aimed to develop a microemulsion formulation of T. procumbens methanolic extract and to evaluate its antibacterial activity against Streptococcus mutans and Enterococcus faecalis, two major oral pathogenic bacteria. The microemulsion was prepared using the sonication method and characterized by assessing its pH, transmittance, viscosity, physical stability, and particle size using a particle size analyzer. Antibacterial activity was tested using the Kirby-Bauer disk diffusion method. The results indicated that the microemulsion had particle sizes ranging from 300–1000 nm and demonstrated significantly higher antibacterial activity compared to the crude extract, suggesting improved solubility and enhanced bioactivity of the active compounds. This formulation holds promise as a natural antibacterial agent for the prevention of oral infections.
Epistemic Agency as a Mediator in Dual-Path Learning: Insights from Indonesian Chemistry Classrooms Abas, Ramona Nintias R.; Musa, Weny J.A.; Laliyo, Lukman Abdul Rauf; Pikoli, Masrid; Kilo, Akram La
Jambura Journal of Educational Chemistry Vol 8, No 1 (2026): February
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jjec.v8i1.34366

Abstract

This study investigates the mediating role of epistemic agency in the relationship between students’ perceptions of constructivist learning environments and their conceptual understanding in Indonesian chemistry classrooms. A sequential explanatory mixed-methods design was employed, integrating quantitative and qualitative approaches. In the quantitative phase, data were collected from 712 high school students across five schools using an adapted Constructivist Learning Environment Survey (CLES) and a three-tier diagnostic chemistry test. Rasch modeling confirmed the strong psychometric properties of the adapted CLES, while structural equation modeling (SEM) revealed that epistemic agency significantly mediated the relationship between classroom environment perceptions and conceptual understanding. In the qualitative phase, thematic analysis of interviews with 12 selected students uncovered three dominant themes contributing to misconceptions: limited dialogic space, lack of contextualization, and teacher-dominated instruction. The integration of both data strands demonstrates that epistemic agency is not only influenced by pedagogical practices but also shaped by cultural norms. These findings extend the dual-path learning framework by embedding agency as a key mechanism for conceptual change. Pedagogically, the results underscore the need for dialogic, student-centered learning environments that promote active participation and reduce misconceptions. Policy-wise, the findings align with the OECD Learning Compass 2030 and Indonesia’s Merdeka Belajar curriculum, emphasizing the importance of agency-oriented strategies in achieving global education standards.
STEM-Integrated Problem-Based Learning as a Cognitively Aligned Framework for Enhancing Critical Thinking in Thermochemistry Condong, Dea Marsanda; Sihaloho, Mangara; Thayban, Thayban; Laliyo, Lukman Abdul Rauf; Musa, Weny J.A.; Kurniawati, Erga; Munandar, Haris; Nggole, Siti Istiqomah
Jambura Journal of Educational Chemistry Vol 8, No 1 (2026): February
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jjec.v8i1.37514

Abstract

Developing students’ critical thinking skills remains a major challenge in chemistry education, particularly in thermochemistry, which requires conceptual, symbolic, and quantitative reasoning. While STEM-integrated problem-based learning (PBL–STEM) has gained increasing attention, limited studies have examined how this instructional approach aligns with the cognitive structure of critical thinking. This study investigated the effect of the PBL–STEM model on students’ critical thinking skills in thermochemistry. A quasi-experimental design involving 64 tenth-grade students was employed. Participants were selected using purposive sampling and assigned to an experimental group and a control group. Critical thinking skills were measured using a 20-item essay test based on Facione’s framework. The instrument demonstrated high reliability (Cronbach’s α = 0.915) and strong content validity (CVI = 0.93). Data were analyzed using an independent-samples t-test. Results showed a significant difference between groups (t = 4.997, p < 0.05), with the experimental group achieving higher mean scores (M = 80.80) than the control group (M = 65.38). The effect size (Cohen’s d = 1.25) indicates a large instructional impact. These findings suggest that STEM-integrated PBL provides a cognitively aligned framework for fostering critical thinking in chemistry learning.
Co-Authors Abas, Ramona Nintias R. Ahmad Kadir Kilo Akram La Kilo Arviani Arviani Boima Ramses Situmeang Condong, Dea Marsanda Erga Kurniawati Haris Munandar La Ode Aman Lukman A. R. Laliyo Mangara Sihaloho Masrid Pikoli Mustafid, Afif Hidayatul Nggole, Siti Istiqomah Nurhayati Bialangi Pradana, Agrin Febrian Prastiwi, Dina Alva Sahami, Ulin Setiyandani, Kurnia Indah Situmeang, Boima Sri Wijayanti Suleman Duengo Thayban, Thayban Wiwin Rewini Kunusa