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STUDI DAYA AKTIVASI ARANG SEKAM PADI PADA PROSES ADSORPSI LOGAM Cd ., Widayanti; Isa, Ishak; Aman, La Ode
Sainstek VOL 06, NO 05, 2012
Publisher : Jurnal Sainstek

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.004 KB)

Abstract

This research aims to find out the ability of activated charcoal rice hurks in the adsorbed metal cadmium in the solvent. The method which is used in this researes are carbonation and activation. The carbonation is doing by close system, the activation is doing by chemical. Activated charcoal produced is tested the absorption of cadmium metal in the solvent. The results showed that activated charcoal which is produced is able to absorb cadmium metal in the solvent that is equal to 19,03 %. The result of proximated analysis of activated charcoal are the water content, volatile matter content, ash content and carbon content are eachboon 13,09%, 15,82%, 53,83% and 30,35%.
Ekspresi IgA dan Rasio CD4+/CD8+ Sel T Mukosa Usus Tikus Setelah Suplementasi Kerang Darah (Anadara granosa) Netty Ino Ischak; La Ode Aman
Global Medical & Health Communication (GMHC) Vol 4, No 2 (2016)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.144 KB) | DOI: 10.29313/gmhc.v4i2.1816

Abstract

Kerang darah (Anadara granosa) mengandung senyawa glikoprotein yang diperkirakan dapat berperan sebagai zat imunomodulator dengan aktivitas tinggi. Penelitian bertujuan mempelajari potensi kerang darah (Anadara granosa) dalam mekanisme sistem imun tikus (Rattus norvegicus) strain Spraque Dawley. Penelitian dilaksanakan di Laboratorium Kimia Jurusan Kimia FMIPA Universitas Negeri Gorontalo dan Laboratorium Primata Bogor IPB selama periode Maret–Oktober 2015. Peranan itu dijelaskan melalui ekspresi imunoglobulin A (IgA) dan skor histologi rasio CD4+/CD8+ sel T mukosa usus secara imunohistokimia. Metode penelitian adalah eksperimental laboratorik dengan menerapkan randomized post test only control group design terhadap 30 ekor tikus yang dipilih secara simple random sampling. Sampel dibagi dalam lima kelompok: (i) kontrol normal; (ii) dan (iii) adalah sampel malnutrisi yang masing-masing mendapat perlakuan ransum nonprotein sebagai kontrol positif [K.kg(+)] dan ransum standar kasein 20% sebagai kontrol negatif [K.kg(−)]; serta (4) dan (5) adalah sampel malnutrisi yang mendapat perlakuan ransum protein kasein 10% yang dikombinasi dengan tepung kerang darah 10% [P.kg1] dan diberi ransum tepung kerang darah 20% [P.kg2]. Perlakuan terhadap subjek selama 45 hari. Kondisi malnutrisi (kadar albumin<2,7 g/dL) diperoleh dengan pemberian ransum nonprotein. Setelah perlakuan, tikus dinekropsi untuk pengambilan jaringan usus. Pemeriksaan IgA dan skor rasio CD4+/CD8+ sel T jaringan mukosa usus (jejunum dan ileum) dengan metode imunohistokimia. Hasil penelitian menunjukkan kerang darah berpotensi meningkatkan kadar IgA, meningkatkan skor histologi CD4+, dan menurunkan skor histologi CD8+ sehingga memengaruhi peningkatan skor rasio CD4+/CD8+ sel T jejunum dan ileum mukosa usus tikus malnutrisi. Simpulan, terdapat peningkatan jumlah ekspresi IgA pada jejunum maupun ileum mukosa usus kelompok yang diberikan suplementasi kerang darah lebih baik dibanding dengan kelompok tanpa diberi suplementasi. IGA EXPRESSION AND CD4+/CD8+ T CELL RATIO OF RAT INTESTINE MUCOSA AFTER ADMINISTRATION OF ANADARA GRANOSAAnadara granosa contains of glycoprotein that has high activity as immunomodulator. The research objective was to study Anadara granosa’s role on immune system mechanism of rats (Rattus norvegicus) Spraque Dawley strain that described by expression of immunoglobulin A (IgA) and histologic score ratio of CD4+/TCD8+ of intestinal mucosa. The study was held in Chemical Laboratory of Chemical Division of FMIPA Universitas Negeri Gorontalo and Laboratory Primata Bogor IPB during March–October 2015. The method was used experimental laboratory with randomized post test only control group design on 30 rats were selected by simple random sampling and divided into five groups: (1) normal controls, (2) and (3) were malnourished subjects to non-protein food as positive control [K.kg(+)], and food standard with casein 20% as negative control [K.kg(−)], (4) and (5) were malnourished subjects thas treated with casein protein diet 10% combined Anadara granosa flour 10% [P.kg1], and given Anadara granosa flour 20% [P.kg2]. The subjects were treated for 45 days. Malnourished condition (albumin<2.7 g/dL), obtained by non-protein diet. Final step, the samples were operated to get intestinal tissues. IgA examination and CD4+/CD8+ rasio of intestine’s mucosal tissues (jejunum and ileum) by immunohistochemically methods. The results showed that Anadara granosa could potentially increased IgA levels and capable to increase histology score of CD4+ and to decrease histology score CD8+. Increasing of CD4+/CD8+ ratio in jejunum and ileum of rats intestinal mucosa with malnourished after be administrated by Anadara granosa flour. In conclusion, IgA expression increased in jejunum and ileum mucosa of supplementation group is better than non-supplementation.
PENAPISAN VIRTUAL BERBASIS STRUKTUR DARI DATABASE BAHAN ALAM ZINC SEBAGAI INHIBITOR BRUTON TYROSINE KINASE Fauzan Zein Muttaqin; Wayan Ayu Puje Astuti; La Ode Aman; Ellin Febrina; Aiyi Asnawi
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 4 No 2 (2019): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.31 KB) | DOI: 10.36387/jiis.v4i2.354

Abstract

Bruton’s tyrosine Kinase (BTK) plays a critical role in many cellular signalling pathways making it a potential target to treat autoimmune diseases and cancer. In this study, we have implemented structure-based virtual screening against natural product ZINC database by using pharmacopore model followed by molecular docking to identified the inhibitor of BTK (PDB ID 6E4F). By using structure based pharmacophore, a four-point pharmacophore hypothesis was derived, with three hydropobic, one aromatic rings, four hydrogen bond acceptor and nine hydrogen bond donor. Screening of 12 natural product ZINC databases (151,837 compounds) against pharmacophore returned 1,345 hits with matching chemical features of 58.81. Docking these hits against the ATP-binding site of the BTK kinase domain through a virtual screening docking-based by using vina wizard and autodock wizard (PyRx 8.0) returned 148 and 75 hits, respectively. Three hit compounds with high affinity towards BTK were identified, and it could be used as a potent lead molecule for designing BTK inhibitor.
Membangun Kewirausahaan Masyarakat Desa Pone Kecamatan Limboto Barat Kabupaten Gorontalo Berbasis Potensi Desa LA ODE AMAN; Ahmad Kadir Kilo; Suleman Duengo
Dedikasi: Jurnal Pengabdian kepada Masyarakat Vol 15 No 1 (2022): Januari - Juni
Publisher : Pusat Pengabdian Kepada Masyarakat Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32678/dedikasi.v15i1.5790

Abstract

KKN MBKM (Kuliah Karya Nyata Merdeka Belajar Kampus Merdeka) is a form of education that provides learning experiences for students to live in the community outside the campus and directly identify community potential and deal with problems so that they are expected to be able to develop the potential of villages and regions and gather solutions to problems that exist in the village. Real Work Lectures at this time coincide with the COVID-19 pandemic, so it has implications for governance differences with normal times. Various work programs have been planned before going to the KKN location, including the manufacture of hydroponic media and aquaponics, making hand sanitizer using natural materials, making Pone's food, and the implementation of tutoring to increase interest in learning from children who have been studying online for a long time. The results of this study show that community service program activities as a form of community service during the pandemic are still ongoing. The community can still feel the benefits, and the role of students can still be carried out with a series of innovative activities as well as continuing to follow health protocols.
Stability Study of Four Layer Aurivillius Oxide of AxBi4-xTi4O15 (A = Ca, Sr, Ba): Atomistic Simulation Akram La Kilo; La Alio; La Ode Aman; Jafar La Kilo
Acta Chimica Asiana Vol. 3 No. 2 (2020)
Publisher : Chemistry Education Program, the University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/aca.v3i2.42

Abstract

Aurivillius is bismuth layered structure ferroelectrics that can be applied as memory, sensor, and catalyst. This research aimed to study the stability of AxBi4-xTi4O15 Aurivillius (A = Ca, Sr, and Ba). Dopants (A) partially substitute Bi at the sites of Bi(1) and Bi(2) of the perovskite layer. This research method is an atomistic simulation using by the GULP code. Simulations were carried out by means of AxBi4-xTi4O15 geometry optimization at constant pressure, using the Buckingham potential. The results showed that the increase in the concentration of dopants substituting Bi accompanied by an increase in lattice energies. The most stable Aurivillius was CaxBi4-xTi4O15 (x = 16.3%) carried out by Bi substitution at Bi(2) site, with lattice energy, -1668.227 eV. Aurivillius stability decreases by increasing the size of the dopant. The maximum concentration number of A dopant substituting Bi was discussed.
Screening of Secondary Metabolite Compounds of Gorontalo Traditional Medicinal Plants Using the In Silico Method as a Candidate for SARS-CoV-2 Antiviral Yuszda K. Salimi; La Ode Aman; Zaenul Wathoni; Netty Ino Ischak; Akram La Kilo; La Alio
Jurnal Kimia Sains dan Aplikasi Vol 25, No 10 (2022): Volume 25 Issue 10 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.25.10.382-393

Abstract

COVID-19 is a disease that caused a prolonged pandemic in many countries caused by the SARS-CoV-2 virus. This study aims to identify the antiviral potential of secondary metabolites in Gorontalo traditional medicinal plants, which are believed to have the ability to inhibit the main protease protein of this virus. The methods used in this research were molecular docking and molecular dynamic. The main protease proteins for SARS-CoV-2 used based on the homology modeling results were 3V3M and 7TE0. The results of the active compounds in the paxlovid drug were also compared to obtain accurate data comparisons. The validation of the docking method on the 3V3M protein using the natural ligand 0EN revealed an RMSD of 0.75 Å. The RMSD value for validating the 7TE0 protein and natural ligand 4WI was 1.65 Å. The best molecular docking results were obtained using physalin F with a binding affinity of −10.3 kcal/mol for the 3V3M protein and physalin J with a binding affinity of −8.9 kcal/mol for the 7TE0 protein. The outcomes of the molecular dynamic method on the best complexes were determined by examining the value of changes in system energy, changes in system temperature, changes in system pressure, RMSD, RMSF, and bond-free energy (ΔG) of the complex. The standard 0EN ligand had a ΔG of −26.53 kcal/mol, while the standard 4WI ligand had a ΔG of −47.16 kcal/mol. The ΔG of the 3V3M-physalin F and 3V3M-physalin J complexes were respectively −28.22 kcal/mol and −26.62 kcal/mol. The ΔG of the 7TE0-Vitexin 2”-O-gallate and 7TE0-physalin J complexes were found to be −28.08 kcal/mol and −26.62 kcal/mol, respectively. The ΔG produced in paxlovid with complexes 3V3M and 7TE0 was −19.38 kcal/mol and −25.44 kcal/mol, respectively. Physalin F, physalin J, and Vitexin 2”-O-gallate have great potential to become SARS-CoV-2 inhibitor agents. However, in terms of structural stability and binding-active residues, these three compounds do not outperform the active substance in paxlovid.
Pencarian Inhibitor DYRK2 dari Database Bahan Alam Zinc15: Analisis Farmakofor, Simulasi Docking dan Dinamika Molekuler La Ode Aman; Mangara Sihaloho; Arfan Arfan
Jurnal Sains Farmasi & Klinis Vol 10, No 1 (2023): J Sains Farm Klin 10(1), April 2023
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.10.1.100-113.2023

Abstract

DYRK2 (Dual-specificity tyrosine phosphorylation-regulated kinase 2) merupakan protein kinase yang memiliki banyak peranan dalam berbagai proses biologis, termasuk pembelahan sel, proliferasi sel, diferensiasi sel, dan apoptosis. DYRK2 diantaranya terlibat dalam regulasi siklus sel dengan cara mengatur aktivitas proteasom 26S sehingga inhibisi aktivitas DYRK2 dapat menghambat fungsi proteasom 26S dan mengurangi proliferasi sel kanker. Secara in vitro, kurkumin menunjukan kemampuan mengurangi proliferasi sel kanker melalui penghambatan enzim DYRK2. Pada penelitian ini, analog kurkumin telah diskrining dari database bahan alam Zinc15 dengan menggunakan model farmakofor yang diperoleg dengan pendekatan berbasis ligan. Hasil skrining kemudian dievaluasi dengan menerapkan teknik docking molekuler dan dinamika molekuler berdasarkan energi interaksi, rata-rata energi pengikatan bebas dan stabilitas interaksi antara ligan dan situs aktif DYRK2. Skrining terhadap 270.547 molekul dari database bahan alam Zinc15 menghasilkan 110 senyawa hit terpilih. Dengan mempertimbangkan hasil simulasi docking dan dinamika molekuler, tiga analog kurkumin prospektif telah dipilih yaitu ZINC000085597244, ZINC000217945958, dan ZINC000217643970. Molekul-molekul ini memiliki kriteria yang lebih baik dibandingkan kurkumin pada beberapa kriteria, seperti energi interaksi, energi pengikatan bebas, dan stabilitas interaksi dengan target. Disimpulkan, senyawa-senyawa ZINC000085597244, ZINC000217945958, dan ZINC000217643970 diprediksi sebagai kandidat potensial untuk obat anti-kanker dengan mekanisme aksi spesifik terhadap DYRK2.
Pengaruh Pembelajaran Guided Inquiry Berbasis Proyek Pada Materi Makromolekul Untuk Meningkatkan Kemampuan Berpikir Kritis Siswa Suchi Wulandari Dai; Netty Ino Ischak; Hendri Iyabu; Lukman A.R Laliyo; La Ode Aman; Haris Munandar
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Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/experiment.v2i2.23034

Abstract

Penelitian ini dilatarbelakangi oleh adanya permasalahan rendahnya kemampuan berpikir kritis siswa dalam pelajaran kimia di kelas XII MIPA SMA Negeri 1 Telaga. Adapun tujuan dari penelitian adalah untuk mengetahui pengaruh pembelajaran guided inquiry berbasis proyek pada materi makromolekul untuk meningkatkan kemampuan berpikir kritis siswa. Penelitian ini dilaksanakan di SMA Negeri 1 Telaga dengan sampel berjumlah masing-masing 36 orang siswa pada kelas kontrol maupun eksperimen. Teknik pengambilan sampel secara purposive sampling. Jenis penelitian yang digunakan adalah Quasi Experimental dengan Nonequivalent Pretest and Posttest Control Group Design. Instrumen tes yang digunakan yaitu tes esai sebanyak 5 butir soal yang mewakili 5 indikator berpikir kritis. Data dianalisis menggunakan uji N-Gain, uji Normalitas, uji Homogenitas, dan pengujian hipotesis (uji-t). Hasil penelitian menunjukkan kemampuan berpikir kritis siswa kelas eksperimen = 81,47 (sangat baik) dan untuk kelas kontrol = 67,95 (baik). Hasil analisis N-Gain (g = 0,77) menyatakan bahwa model pembelajaran Guided Inquiry berbasis proyek memiliki kriteria sedang. Hipotesis diuji menggunakan uji-t,  menunjukkan thitungttabel (57,72 1,99). Dapat disimpulkan bahwa terdapat pengaruh pembelajaran Guided Inquiry berbasis proyek pada materi makromolekul berpendekatan SETS untuk meningkatkan kemampuan berpikir kritis siswa di SMA Negeri 1 Telaga. 
Isolasi Senyawa Metabolit Sekunder dan Uji Aktivitas Antioksidan Ekstrak Etil Asetat Daun Bidara (Ziziphus mauritiana) Bialangi, Nurhayati; Sahami, Ulin; Musa, Weny J.A.; Kunusa, Wiwin Rewini; Aman, La Ode
Jambura Journal of Chemistry Vol 5, No 1 (2023): February
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jambchem.v5i1.16022

Abstract

Penelitian ini bertujuan mengisolasi dan mengidentifikasi senyawa metabolit sekunder serta mengetahui aktivitas antioksidan yang terkandung dalam ekstrak etil asetat daun Z. mauritiana. Tahap isolasi diawali dengan ekstraksi secara maserasi menggunakan pelarut etil asetat. Uji fitokimia menunjukkan ekstrak etil asetat positif mengandung flavonoid, tanin dan terpenoid. Proses pemisahan dilakukan menggunakan kromatografi lapis tipis dengan campuran eluen n-heksana : etil asetat (9:1 dan 8:2) dilanjutkan dengan kromatografi kolom. Fraksi-fraksi yang diperoleh dilakukan proses pemurnian menggunakan metode rekristalisasi. Isolat nomor 14 diduga murni berupa kristal jarum berwarna hijau kekuningan sebanyak 4,5 mg. Uji kemurnian menggunakan KLT dua dimensi dengan eluen pertama n-heksana : etil asetat (8:2) dan eluen kedua kloroform : metanol (9:1) diperoleh satu bercak noda tunggal. Spektrum UV-Vis menunjukkan adanya gugus kromofor tak jenuh C=C (251 nm) dan kromofor C=O (283 nm). Sedangkan, spektrum IR menunjukkan adanya gugus ulur OH gugus, ulur C-H alifatik, gugus tekuk C-H, gugus fungsi C=O, C=C dan gugus fungsi ulur C-O. Hasil uji aktivitas antioksidan menunjukkan bahwa kemampuan reduktif dari ekstrak daun Z. mauritiana yang berbeda secara signifikan jika dibandingkan dengan standar vitamin C. Ekstrak daun Z. mauritiana memiliki aktivitas lebih rendah (IC50 489.9936 "μg/ml" ) dibandingkan dengan standar vitamin C.
Studi Molecular Docking dan Prediksi ADME Senyawa Metabolit Sekunder Tumbuhan Obat Tradisional Gorontalo Terhadap Reseptor HER-2 Sebagai Antikanker Payudara Ischak, Netty Ino; Musa, Weny J.A; Aman, La Ode; Alio, La; Kilo, Akram La; Saleh, Sri Deltalia
Jambura Journal of Chemistry Vol 5, No 2 (2023): August
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jambchem.v5i2.20544

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas dan interaksi senyawa uji dari 12 tumbuhan obat tradisional Gorontalo dengan reseptor HER-2 yang merupakan protein yang terkait dengan sel kanker payudara secara in silico melalui molecular docking. Molekul dengan hasil docking terbaik akan diprediksi menggunakan program Absobsi, Distribusi, Metabolisme, dan Eliminasi (ADME) menggunakan program SwissADME secara online. Hasil validasi situs ikatan dengan menerapkan protokol penambatan ulang (redocking) menunjukkan nilai Root Mean Square Deviation konformasi ligan standar (2-{2-[4-({5-chloro-6-[3-(trifluoromethyl)phenoxy]pyridine-3-yl}amino)-5H-pyrrolo[3,2-d]pyrimidin-5yl]ethoxy}ethanol) menggunakan Autodock Tools dengan nilai energi bebas ikatan yang terbentuk sebesar -6,32 kkal/mol. Sedangkan nilai Root Mean Square Deviation ligan standar menggunakan Autodock Vina memiliki energi bebas ikatan sebesar -11,3 kkal/mol. Hasil energi bebas ikatan ligan uji Palmarumycin CP1 sebesar -9,2 kkal/mol. Interaksi residu aktif HER-2 pada Palmarumycin CP1 dengan kontribusi terbesar terdapat pada residu Tyr 735. Hasil prediksi ADME menunjukkan bahwa hanya ada lima senyawa yang termasuk aturan Lipinski Rule of Five yaitu senyawa Tembetarine, Palmarumycin CP1, Curcumin, Cepharadione A, dan Hexahydrocurcumin. Oleh karena itu, hasil ADME menunjukkan bahwa hanya ada satu senyawa yang memenuhi aturan Lipinski Rule of Five dan sesuai dengan hasil Molecular Docking yang memiliki energi bebas ikatan sebesar -9,2 kkal/mol adalah senyawa Palmarumycin CP1.