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All Journal Reaktor Indonesian Journal of Chemistry
Puspitasari, Nathania
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Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Energy Materials Science & Nanotechnology

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Heterogeneous Fenton Degradation of Batik Wastewater using Natural Pyrite Irawaty, Wenny; Yuliana, Maria; Wijaya, Christian J.; Retnoningtyas, Ery S.; Lourentius, Suratno; Puspitasari, Nathania; Hamidah, Umi
Reaktor Volume 23 No.3 December 2023
Publisher : Department of Chemical Engineering, Faculty of Engineering, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.23.3.101-107

Abstract

Heterogeneous Fenton processes with solid natural pyrite catalyst have gained attention for wastewater treatment. The improvement of degradation efficiency of natural pyrite is specific and therefore, it is still a challenging issue. This study was aimed to investigate the performance of Indonesian natural pyrite, without any purification, to treat the real wastewater obtained from batik home industry. To address this issue, several experiment variables such as pre-reaction between pyrite and hydrogen peroxide, hydrogen peroxide concentration, and pH were investigated. COD removal has been found to increase in a slightly acid condition (4.5 to 5.65) and inhibited at pHs of 8 and above (10). The results showed the presence of hydrogen peroxide was indeed greatly influence the wastewater degradation and the solution pH did not significantly change the wastewater degradation in the range of 3 and less than 8. Moreover, the pre-reaction period was found as the important part to initiate the degradation process. The optimum degradation efficiency was obtained by providing the pre-reaction time of 20 min with the addition of 326 mmol/L of H2O2 in the system and the mixture was reacted for 30 min at room temperature and real wastewater pH. Based on results observed in this study, it can be concluded that the batik wastewater can be degraded by natural pyrite up to 84% under reaction conditions studied. This finding offers great opportunity to further investigate and develop the heterogeneous Fenton process for real applications in industry.
Pseudoternary Phase Diagram and Antibacterial Activity of Microemulsion-Based Citronella Oil Gunarto, Chintya; Go, Alchris Woo; Angkawijaya, Artik Elisa; Lie, Jenni; Soetaredjo, Felycia Edi; Ismadji, Suryadi; Puspitasari, Nathania; Putro, Jindrayani Nyoo; Risdian, Chandra
Indonesian Journal of Chemistry Vol 24, No 3 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.93250

Abstract

Citronella oil (CTO) is extracted from citronella leaves by maceration or steam distillation process, which has antibacterial and insect-repellent activities. However, the use of CTO is limited and requires modification in other formulations, such as microemulsion (ME), to increase its bioactivities. ME consists of oil, water, surfactant and/or cosurfactant and is commonly applied in food and beverages, cosmetics, and carrier for drug delivery applications. CTO was used as the oil phase for ME with nonionic surfactant and ethanol as a cosurfactant for lowering interfacial tension between oil and water phase. Subsequent observations regarding stability and antibacterial tests were carried out on ME formulations with surfactant/cosurfactant mixture of 2 due to its largest ME area. A hydrodynamic diameter analysis was also carried out to see the stability of the ME within a period of 50 d. ME with 10% CTO, 30% surfactant mixture, and 60% water showed the best formulation observed from the consistent hydrodynamic diameter measurement. In addition, ME with different formulations could inhibit the growth of Escherichia coli and Staphylococcus aureus by more than 90%. From this research, CTO-based ME potentially improve and develop drug carrier applications, especially via topical route.
Optimum and Green Fabrication of MIL-100(Fe) for Crystal Violet Dye Removal from Aqueous Solution Wijaya, Christian Julius; Soetaredjo, Felycia Edi; Yuliana, Maria; Santoso, Shella Permatasari; Hartono, Sandy Budi; Irawaty, Wenny; Lie, Jenni; Putro, Jindrayani Nyoo; Gunarto, Chintya; Puspitasari, Nathania; Ismadji, Suryadi; Gunawan, Setiyo
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.93323

Abstract

MIL-100(Fe) was prepared and subsequently used to remove crystal violet dye from aqueous solutions simulating dye-containing wastewater in the environment. In the future, it is aimed that MIL-100(Fe) can be used in managing dye-containing wastewater in the environment and reducing the negative impacts it can cause. Here, MIL-100(Fe) fabrication needs to be optimized to obtain optimum process conditions, which are environmentally friendly and can produce MIL-100(Fe) with the best characteristics. This study focused on optimizing the fabrication of MIL-100(Fe), which is a type of MOF with good chemical stability, thermal stability, and flexible structure. In this study, the room-temperature fabrication of MIL-100(Fe) was established using a ligand-to-metal molar ratio of 0.95 and an acetic acid concentration of 5.1 vol% for 6.2 h. The optimum MIL-100(Fe) was tested for crystal violet removal and provided an optimum removal capacity of 182.66 ± 3.81 mg/g. Statistical approaches are used to investigate the independent parameters and their interactions contributing to MIL-100(Fe) formation.
Accelerated Biodegradation of Waste Polyethylene Terephthalate (PET) Fiber by Recombinant Hydrophobin HGFI Fused with Ideonella sakaiensis PETase Puspitasari, Nathania; Liu, Chia Tzu; Lee, Cheng Kang
Indonesian Journal of Chemistry Vol 25, No 5 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.93092

Abstract

Nowadays, an efficient and environmentally friendly polyethylene terephthalate (PET) recycling method has gained greater attention, enabling a circular economy for polyesters. Since PET is a hydrophobic polymer, the enzyme's binding affinity to PET becomes a significant issue. Herein, the first strategy is introduced to enhance PET fiber hydrolysis by genetically fusing hydrolyzing enzymes such as cutinase and PETase to protein hydrophobin HGFI. HGFI, a surface-active protein from Grifola frondosa, was used to improve the rate of enzyme hydrolysis. Furthermore, cellulose binding domains (CBD) were employed as a solubility enhancer tag of PETase fusion due to the insoluble characteristics of HGFI and PETase. The fusion proteins (CBD-HGFI-PETase and HGFI-Cut_2) were constructed with a flexible linker, expressed in Escherichia coli, and then purified by chromatography. PETase fusion exhibited 2.5-fold higher concentrations of monomer products released than that of cutinase fusion after 5 days of hydrolysis. According to the results, the fusion of HGFI to PETase showed excellent performance for enhancing the binding affinity of the enzyme on PET fiber substrate due to the increasing number of self-assembled hydrophobin interactions that modified the PET surface to be more hydrophilic. Therefore, this study indicates that the construction of CBD-HGFI-PETase enzyme fusion could be used as a novel method for efficiently accelerating PET biodegradation.
Co-Authors Angkawijaya, Artik Elisa Chandra Risdian Felycia Edi Soetaredjo Go, Alchris Woo Gunarto, Chintya Hamidah, Umi Hartono, Sandy Budi Lee, Cheng Kang Lie, Jenni Liu, Chia Tzu Lourentius, Suratno Putro, Jindrayani Nyoo Retnoningtyas, Ery S. Santoso, Shella Permatasari Setiyo Gunawan Suryadi Ismadji Wenny Irawaty Wijaya, Christian J. Wijaya, Christian Julius Yuliana, Maria