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All Journal Indonesian Journal of Chemistry Widya Teknik
Angkawijaya, Artik Elisa
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Chemical Engineering, Chemistry & Bioengineering Chemistry

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Pseudoternary Phase Diagram and Antibacterial Activity of Microemulsion-Based Citronella Oil Gunarto, Chintya; Go, Alchris Woo; Angkawijaya, Artik Elisa; Lie, Jenni; Soetaredjo, Felycia Edi; Ismadji, Suryadi; Puspitasari, Nathania; Putro, Jindrayani Nyoo; Risdian, Chandra
Indonesian Journal of Chemistry Vol 24, No 3 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.93250

Abstract

Citronella oil (CTO) is extracted from citronella leaves by maceration or steam distillation process, which has antibacterial and insect-repellent activities. However, the use of CTO is limited and requires modification in other formulations, such as microemulsion (ME), to increase its bioactivities. ME consists of oil, water, surfactant and/or cosurfactant and is commonly applied in food and beverages, cosmetics, and carrier for drug delivery applications. CTO was used as the oil phase for ME with nonionic surfactant and ethanol as a cosurfactant for lowering interfacial tension between oil and water phase. Subsequent observations regarding stability and antibacterial tests were carried out on ME formulations with surfactant/cosurfactant mixture of 2 due to its largest ME area. A hydrodynamic diameter analysis was also carried out to see the stability of the ME within a period of 50 d. ME with 10% CTO, 30% surfactant mixture, and 60% water showed the best formulation observed from the consistent hydrodynamic diameter measurement. In addition, ME with different formulations could inhibit the growth of Escherichia coli and Staphylococcus aureus by more than 90%. From this research, CTO-based ME potentially improve and develop drug carrier applications, especially via topical route.
Antibacterial Activity and Polyphenols Content of Methanolic Extract of Sapindus Rarak Murni, Asmara; Rohman, Aufur; Angkawijaya, Artik Elisa; Santoso, Shella Permatasari
Widya Teknik Vol. 22 No. 2 (2023): November
Publisher : Fakultas Teknik, Universitas Katolik Widya Mandala Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/wt.v22i2.5409

Abstract

The study focused on the methanolic extract of lerak fruit (Sapindus rarak DC.). The effect of varying extraction time and temperature on the polyphenols content of lerak extract was evaluated, that is by determining the total phenolic content (TPC), total flavonoid content (TFC), and total saponin content (TSC). The increasing extraction temperature from 30°C to 70°C was found to give increase in TPC from 172 mg GAE/100 mg to 246 mg GAE/100 mg, further increase of extraction temperature to 90°C results in the decrease of TPC. Similar effect was also observed in TFC, where TFC was found to increase as the extraction temperature was increased to 70°C, with TFC of 79 mg QE/100 mg. TSC of lerak fruit extract at 70°C was found to be 17.6 mg/100 g. Antibacterial activity assay on the lerak fruit extract shows the potential inhibitory activity of the extract on the growth of Escherichia coli and Staphylococcus aureus, wherein 95% inhibition efficiency can be achieved after the incubation of the bacteria in media containing 15 wt.% of the extract. The more prominent inhibitory effect of the extract was shown against Escherichia coli than Staphylococcus aureus. Collectively, the results of this study has demonstrated the potential of lerak fruit extract as natural antibacterial agent with foaming ability, which can be used as detergent additive.
Co-Authors Chandra Risdian Felycia Edi Soetaredjo Go, Alchris Woo Gunarto, Chintya Lie, Jenni Murni, Asmara Puspitasari, Nathania Putro, Jindrayani Nyoo Rohman, Aufur Santoso, Shella Permatasari Suryadi Ismadji