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Ethnobotany Medicinal Plants of Dawan Ethnic in North Central Timor Regency Noviana Mery Obenu; Emilia Juliyanti Bria
Biotropika: Journal of Tropical Biology Vol. 9 No. 3 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.03.09

Abstract

The use of medicinal plants is still maintained for generations by the Dawan Tribe in North Central Timor Regency. The loss of knowledge about the use of plants as an alternative treatment of various diseases used by local communities can be caused by the impact of modernization. Therefore, it is necessary to carry out the process of documenting this knowledge. The purpose of this research is to take an inventory of medicinal plants, to know the plant organs used for medicine and how to use the medicine. The method used in this study is a qualitative description method, with several field data collection techniques and identification of medicinal plant species. Data collection techniques include observation, interviews, and exploration. The results of this study indicate that there are 44 species of medicinal plants used, from 25 plant families. The medicinal plant family with a high number of species is the Zingiberaceae family (13.6%). Based on the habitus, 44 species of medicinal plants used consisted of 23 species from trees, 2 species from lianas, 9 species from herbs, 7 species from shrubs, and 3 species from shrubs. In addition, based on plant organs the most widely used as medicine out of 44 plant species, namely the part of the leaf organ (23.91%). Meanwhile, based on the method of use of the 44 species of plants used for medicinal ingredients, the method of drinking has the highest percentage (33.33%) and is usually practiced by the Dawan Tribe.
Ekstraksi dan Identifikasi Komposisi Metabolit Fraksi Diklorometana dan Aquades Ektrak Metanol Daun Sirsak (Annona muricata Linn) Noviana Mery Obenu
Saintek Lahan Kering Vol 2 No 1 (2019): JSLK Juni 2019
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.971 KB) | DOI: 10.32938/slk.v2i1.717

Abstract

Soursop is one of the many plants found in East Nusa Tenggara (NTT). Some parts of the soursop plant are known to have been widely used as traditional medicine or as natural pesticides. The use of plants as a treatment or as a natural pesticide cannot be separated from the bioactive compounds contained therein. These compounds will be successfully isolated depending on the choice of solvent. This research was conducted with the aim of knowing the composition of the metabolites contained in each solvent used. The steps of this study include preparation of soursop leaf samples, extraction by maceration with methanol solvents, partitioning of methanol extract with dichloromethane and water solvents and identification of metabolite compositions with high-performance liquid chromatography (HPLC) instruments. The compounds identified in HPLC dichloromethane solvents are thought to be phenol, polyketide, tannin and alkaloid metabolites. Whereas HPLC identified compounds in water solvents are thought to be polar compounds which are bound to a glycoside group
Identification Chemical Compositions of Lemongrass Plant (Cymbopogon nardus L.) Dawan Tribe, Oenenu Village, North Central Timor Regency Noviana Obenu; Eduardus Edi; Risna E. Adu
Jurnal Akademika Kimia Vol. 10 No. 2 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.673 KB) | DOI: 10.22487/j24775185.2021.v10.i2.pp93-97

Abstract

Lemongrass (Cymbopogon nardus L.) is a local natural resource used by the Dawan tribe as an alternative for mosquito repellent. The utilization of these plants cannot be separated from the content of compounds found in these plants. Therefore, it is necessary to analyze the content of bioactive compounds from lemongrass (Cymbopogon nardus L.). This research method includes sample preparation, isolation and identification of compounds, and antioxidant activity analysis. The results of this study that there were three main components of citronella oil, namely geraniol, citronellol, and citronellal, with a higher percentage of geraniol, namely 18.82%. The compounds contained in lemongrass oil have a weak antioxidant activity with IC50 681.48 mg/mL.
PENGARUH PRETREATMENT MAKROALGA ULVA RETICULATA MENGGUNAKAN MICROWAVE IRRADIATION UNTUK PRODUKSI BIOETANOL S. M. D. Kolo; N. M. Obenu; M. Y. C. Tuas
Jurnal Kimia (Journal of Chemistry) Vol. 16, No.2, Juli 2022
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2022.v16.i02.p12

Abstract

Produksi bahan bakar fosil setiap tahun hingga saat ini mengalami penurunan yang signifikan. Hal ini seiring dengan peningkatan konsumsi energi secara signifikan sebagai akibat bertambahnya populasi penduduk, pertumbuhan ekonomi, akitivtas industri dan transportasi. Energi alternatif yang dapat digunakan sebagai pengganti bahan bakar fosil adalah pemanfaatan makroalga Ulva reticulata menjadi bioetanol. Kandungan lignoselulosa Ulva reticulata dapat dikonversi menjadi etanol melalui proses pretreatment dan hidrolisis asam encer menggunakan microwave irradiation, yang kemudian dilanjutkan dengan proses fermentasi dan distilasi. Morfologi permukaan sampel makroalga Ulva reticulata dianalisis menggunakan Scanning Electron Microscopy (SEM). Gula pereduksi dianalisis menggunakan metode Dinitrosalisilat (DNS). Kadar etanol dianalisis menggunakan Gas Chromatography-Flame Ionization Detector (GC-FID). Hasil penelitian menunjukkan bahwa proses pretreatment dan hidrolisis asam encer menggunakan microwave irradiasi mencapai waktu dan suhu hidrolis yang optimum pada 50 menit dan suhu 1500C, dengan konsentrasi gula pereduksi sebesar 27,97 g/L dan efisisensi hidrolisisnya sebesar 69,93%. Hasil analisis kadar etanol Ulva reticulata menggunakan GC-FID adalah 7,76%. Produksi bioetanol dari makroalga Ulva reticulata diharapkan menjadi sumber baru energi terbarukan yang potensial karena tidak digunakan sebagai bahan pangan. Hasil penelitian ini sebagai sumber informasi baru bagi industri untuk meningkatkan nilai ekonomi dari makroalga Ulva reticulata. Kata Kunci: bioetanol, fermentasi, microwave irradiasi, pretreatment, ulva reticulata. ABSTRACT The production of fossil fuels every year until now has decreased significantly. This is in line with the significant increase in energy consumption as a result of increasing population, economic growth, industrial and transportation activities. Alternative energy that can be used as a substitute for fossil fuels is the use of macroalgae Ulva reticulata into bioethanol. The lignocellulosic content of Ulva reticulata can be converted into ethanol through a pretreatment process and dilute acid hydrolysis using microwave irradiation, which is then followed by a fermentation and distillation process. Surface morphology of Ulva reticulata macroalgae samples were analyzed using Scanning Electron Microscopy (SEM). Reducing sugars were analyzed using the Dinitrosalicylate (DNS) method. Ethanol content was analyzed using Gas Chromatography-Flame Ionization Detector (GC-FID). The results showed that the pretreatment and hydrolysis of dilute acid using microwave irradiation reached the optimum hydraulic time and temperature of 50 minutes and 150, with a reducing sugar concentration of 27.97 g/L and a hydrolysis efficiency of 69.93%. The results of the analysis of the ethanol content of Ulva reticulata fermentation using GC was 7.76%. Bioethanol production from the macroalga Ulva reticulata is expected to be a potential new source of renewable energy because it is not used as food. The results of this study serve as a new source of information for the industry to increase the economic value of the macroalga Ulva reticulata. Keywords: bioethanol, fermentation, microwave irradiation, pretreatment, ulva reticulata.
Permainan Sains Menggunakan Bahan Sederhana bagi Siswa/i SMP Negeri II Koko Kecamatan Kota Kefamenanu, Kabupaten TTU Noviana Obenu; Janrigo Klaumegio Mere; Gebhardus D. Gelyaman; Emilia Juliyanti Bria; Yoseph Satrio Akoit; Patrisius Maryanto Bria
Jurnal Abdi Masyarakat Indonesia Vol 2 No 6 (2022): JAMSI - November 2022
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.506

Abstract

Pendidikan yang baik adalah pendidikan yang dapat meningkatkan daya literasi, daya belajar, inovasi dan kreatifitas siswa. Salah satu cara dalam penyampaian proses belajar mengajar yang lebih optimal adalah dengan penyiapan media belajar yang menarik. Media belajar dapat dilakukan dengan pertunjukkan permaianan sains. Kegiatan pengabdian ini dilaksanakan di SMP Negeri II Koko dengan dihadiri siswa/I kelas VII, VIII, dan IX. Materi pengabdian berupa permainan sains berupa Pesan rahasia, Magnet listrik, Peristiwa melayang tenggelam dan terapung, Gunung meletus dan Listrik buah. Kegiatan permainan atau demonstrasi sains ini dilakukan untuk memperkenalkan kepada siswa-siswi pembelajaran sains yang lebih menarik. Kegiatan ini dilaksanakan dengan metode pertunjukkan, ceramah dan diskusi. Dapat disimpulkan bahwa siswa – siswi berinteraksi secara aktif, tidak kaku, penuh semangat ditandai dengan banyaknya pertanyaan yang muncul, dan siswa sangat antusias dalam menjawab pertanyaan yang diberikan oleh tim pengabdi serta tingginya minat siswa untuk terlibat di dalam praktik demonstrasi sains.
PELATIHAN PEMBUATAN HERBARIUM KERING SEBAGAI MEDIA PEMBELAJARAN DI SEKOLAH MENENGAH PERTAMA NEGERI 2 KEFAMENANU Emilia Juliyanti Bria; Noviana Mery Obenu; Janrigo Kalumegio Mere
Jurnal Pasopati Vol 5, No 1 (2023)
Publisher : Fakultas Teknik Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/pasopati.2023.17369

Abstract

Media pembelajaran merupakan salah satu komponen penting dalam proses belajar-mengajar. Salah satu media pembelajaran yang mendukung pembelajaran berbasis lingkungan di tingkat sekolah menengah pertama khususnya metapelajaran IPA adalah herbarium. Tujuan pengabdian ini adalah memberikan pengetahuan tentang herbarium dan keterampilan bagaimana teknik membuat herbarium secara sederhana bagi siswa di SMP Negeri 2 Kefamenanu. Metode yang digunakan adalah tutorial dan pendampingan secara kelompok kepada siswa. Hasil kegiatan ini menunjukkan bahwa siswa-siswi sangat antusias dalam mengikuti pelatihan yang ditunjukkan dengan diperolehnya pengetahuan dan keterampialn baru tentang herbarium dan teknik membuatnya secara sederhana. Selain itu, diperoleh juga sebelas spesies tumbuhan yang teridentifikasi nama ilmiahnya yang dibuat herbarium dari lingkungan sekolah dan dapat dijadikan sebagai perangkat pembelajaran dalam menunjang tercapainya tujuan pembelajaran.Kata kunci : pelatihan, herbarium, media pembelajaran, Kefamenanu
Optimasi Proses Hidrolisis Rumput Laut Ulva Reticulata dengan Pelarut HNO3 untuk Produksi Bioetanol Sefrinus Maria Dolfi Kolo; Noviana Mery Obenu; Lusitania Kefi; Felicitas F.Fuel
Jurnal Riset Kimia Vol. 14 No. 1 (2023): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v14i1.574

Abstract

One alternative to make biofuels and replace petroleum-based fuels is to convert non-food ingredients from Ulva reticulata seaweed into bioethanol. Seventy percent of the earth's surface is covered by microalgae and seaweeds that can be converted into bioethanol. Ulva seaweed contains 50.3% carbohydrates in the form of heteropolysaccharides  such as glucose, arabinose, rhamnose and xylose. Optimization of the seaweed hydrolysis catalyzed by HNO3 using Microwave irradiation was done by varying acid concentration (1, 3, 5, 7%), hydrolysis time (30, 40, 50, and 60 minutes), and hydrolysis temperature (75, 100, 125, 150°C). Fermentation was carried out by varying inoculum concentrations (6, 8, and 10% (v/v)) for 5, 6 and 7 days at a temperature of 30°C and a pH of 4.5. Analysis of the surface texture of the sample was carried out by Scanning Electron Microscopy (SEM). The analysis of reducing sugars concentration was carried out using the dinitrosalicylate (DNS) method. Ethanol analysis was carried out by Gas Chromatography (GC). The results of SEM analysis showed that prior to hydrolysis, the surface morphology of the powder was still compact and intact. Whereas after being hydrolyzed with HNO3 it was seen that the surface texture of the powder suffered significant damage. The hydrolysis results showed that the optimum conditions during the pretreatment of U. reticulata powder was at acid concentration of 7%, reaction time of 50 minutes, reaction temperature of 150°C and 250 watts of power which gave hydrolysate with reducing sugar concentration of 86.5 g/L. Fermentation of the hydrolysate using yeast Saccharomyces cerevisiae produced bioethanol with concentration of 37.2% as analyzed using a gas chromatograph.
Analisis Fitokimia Ekstrak Pelarut Kulit Akar Tumbuhan “At Anonse” (Annona reticulata L.) Maria Kasilda Elu; Oktovianus Kasa; Maria Aprista Manikin; Noviana Mery Obenu; Eduardus Edi
Saintek Lahan Kering Vol 6 No 1 (2023): JSLK JUNI 2023
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/slk.v6i1.2188

Abstract

Medicinal plants are plants that can be used as medicine or medicinal ingredients to treat various diseases. One of the plants used as a medicinal plant is Annona reticulata L. This study aims to determine the content of secondary metabolites in the solvent extract of the root bark of the plant “At Anonse” (Annona reticulata L.). The stages of this research were sample preparation, extraction, and phytochemical analysis. Extraction was carried out by maceration using n-hexane, ethyl acetate, and methanol as solvents. Phytochemical analysis was carried out qualitatively based on changes in color, precipitate and foam. The results of the phytochemical analysis test showed that the groups of compounds contained in the root bark of the plant “At Anonse” were triterpenoids, tannins, flavonoids, phenolics, alkaloids and saponins.
Identification Chemical Compositions of Lemongrass Plant (Cymbopogon nardus L.) Dawan Tribe, Oenenu Village, North Central Timor Regency Noviana Obenu; Eduardus Edi; Risna E. Adu
Jurnal Akademika Kimia Vol. 10 No. 2 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24775185.2021.v10.i2.pp93-97

Abstract

Lemongrass (Cymbopogon nardus L.) is a local natural resource used by the Dawan tribe as an alternative for mosquito repellent. The utilization of these plants cannot be separated from the content of compounds found in these plants. Therefore, it is necessary to analyze the content of bioactive compounds from lemongrass (Cymbopogon nardus L.). This research method includes sample preparation, isolation and identification of compounds, and antioxidant activity analysis. The results of this study that there were three main components of citronella oil, namely geraniol, citronellol, and citronellal, with a higher percentage of geraniol, namely 18.82%. The compounds contained in lemongrass oil have a weak antioxidant activity with IC50 681.48 mg/mL.
Analisis Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Pelarut Kulit Batang Tumbuhan “At Anonse” (Annona reticulata L.) Obenu, Noviana Mery; Bria, Emilia Juliyanti; Kolo, Maria Magdalena; Mere, Jenrigo Klaumegio; Tse, Anastasia
Akta Kimia Indonesia Vol 9, No 1 (2024)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v9i1.17399

Abstract

Antioksidan adalah senyawa yang berfungsi untuk melawan radikal bebas dan melindungi sel dari efek toksik yang dihasilkan. Sumber antioksidan dapat berasal dari dalam dan luar tubuh. Antioksidan yang berasal dari luar tubuh dapat diperoleh dari tanaman yang mengandung senyawa metabolit sekunder seperti senyawa fenolik, flavonoid, alkaloid, tanin, steroid dan terpenoid. Berdasarkan hasil eksplorasi dan identifikasi tumbuhan Annona reticulata L. juga dimanfaatkan sebagai tumbuhan obat. Penelitian ini dilakukan dengan tujuan untuk mengetahui senyawa metabolit sekunder dalam ekstrak tumbuhan dan aktivitas antioksidannya. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan metanol sedangkan uji aktivitas antioksidan di lakukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Hasil analisis fitokimia menunjukkan bahwa golongan senyawa yang terkandung dalam ekstrak etil asetat dan metanol adalah golongan triterpenoid, tanin, saponin, fenolik, flavonoid, dan alkaloid. Aktivitas antiosidan kedua ekstrak pelarut tersebut tergolong dalam kategori sedang dengan nilai IC50 ekstrak etil asetat sebesar 177,1325 μg/mL dan metanol sebesar 235,4198 μg/mL.