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All Journal Jurnal Kedokteran dan Kesehatan Science Midwifery
Samosir, Sarah Christina
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Dentistry Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Kadar TNF-α Enkapsulasi Sel Punca Mesenkimal Sufida, Sufida; Sibuea, Christine Verawaty; Silaen, Rachel Teodora; Kuara, Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora
Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2024): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.20.2.119-123

Abstract

Sel punca mesenkimal (MSCs) memiliki kemampuan proliferasi dan kemampuan berdiferensiasi yang tinggi, dan efek parakrin yang mengandung banyak faktor pertumbuhan dan sitokin pro-inflamasi. Tumor necrosis factor-α (TNF-α) merupakan salah satu faktor pertumbuhan yang disekresikan oleh MSCs. TNF-α memiliki peran penting dalam pengaturan sistem imun. Terapi seluler penyakit degeneratif dan infeksi menggunakan MSCs memanfaatkan kemampuan proliferasi, diferensiasi dan efek parakrinnya. Kematian sel sebelum mencapai organ target merupakan keterbatasan dalam terapi seluler. Penelitian menggunakan pendekatan enkapsulasi MSCs dikembangkan untuk mengatasi keterbatasan kurangnya retensi MSCs pada terapi seluler dalam mempertahankan kelangsungan hidup dan efek parakrin MSC. Penelitian ini bertujuan untuk mengetahui efek parakrin MSCs berupa kadar TNF-α pada enkapsulasi MSCs. MSCs dienkapsulasi dengan menggunakan alginat yang dicross-linked dengan CaCl2. Enkapsulasi MSCs dikultur selama 21 hari dan dilakukan analisis kadar TNF-α. Penelitian ini menunjukkan bahwa TNF-α pada MSCs mengalami penurunan hingga hari ke-21. Hal ini menunjukkan bahwa enkapsulasi MSCs mempengaruhi kadar TNF-α dan mempertahankan MSCs dari lingkungan luar.
TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Silaen, Rachel Teodora; Kuara , Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora; Yana, Hiqmah Yusi; Pratama, Gita; Mutiara, Mutiara; Angeline, Wiedya Kristianti
Science Midwifery Vol 11 No 6 (2024): February: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i6.1427

Abstract

Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule
Co-Authors Angeline, Wiedya Kristianti Christine Verawaty Sibuea Ginting, Kharnis Marsha Madora Gita Pratama Kuara , Glenessa Kuara, Glenessa mutiara mutiara Silaen, Rachel Teodora Simaremare, Ade P Sitanggang, Ervina Julien Sufida, Sufida Yana, Hiqmah Yusi