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Novel Endophytic Bacteria Isolates from Andaliman (Zancthoxylum acanthopodium DC.) which Potentially Inhibit Escherichia coli and Staphylococcus aureus Rizqoh, Debie; Amelia, Aisyah; Kumala, Wulan Okta; Sipriyadi, Sipriyadi; Suryani, Utari Hartati; Sariyanti, Mardhatillah
Journal of Biomedicine and Translational Research Vol 10, No 1 (2024): April 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i1.21740

Abstract

Background: Irrational use of antibiotics has various side effects to resistance. Utilization of secondary metabolites of Andaliman plant endophytic bacteria (Z. acanthopodium DC) is an alternative known to act as an antibacterial to overcome this problem.Objective: This study aimed to determine the antimicrobial activity of the crude extract of Andaliman endophytic bacteria against Escherichia coli and Staphylococcus aureus.  Methods: This research is an experimental laboratory with qualitative data collection methods. The initial stages will be the isolation of endophytic bacteria from Andaliman, the antagonist test of Andaliman endophyte isolates against E. coli and S. aureus, and the extraction of endophytic bacterial isolates using ethyl acetate as solvent. An antagonistic test of bacterial isolate extract was performed using the disc diffusion method against pathogenic bacteria E. coli and S. aureus and observed by an inhibition zone. The final stage is the minimum inhibition test of endophytic isolates by dilution method on three potential endophytic isolates.Results: The results of the extraction of isolates EAA22, EAA28, EAB5, EAB6, and EAB7 Andaliman endophytic bacteria have inhibitory activity against S.aureus, and the results of the extraction of isolates EAB5, EAB6, EAA22 have inhibitory activity against E. coli.Conclusion: Endophyte bacteria isolates from Andaliman produce antibacterial compounds against E. coli and S. aureus. 
Pelatihan pembuatan hand sanitizer sebagai antiseptik berstandar WHO Oktoviani, Oktoviani; Rizqoh, Debie; Handayani, Dian; Rachmawati, Indah Dwi; Kumala, Wulan Okta
Jurnal PADAMU NEGERI (Pengabdian pada Masyarakat Bidang Eksakta) Vol 2, No 2 (2021)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.844 KB) | DOI: 10.37638/padamunegeri.v2i2.437

Abstract

The incessant socialization of the government regarding health protocols to a new life order has resulted in most Indonesians becoming introspective in maintaining cleanliness. However, some of the community also experienced panic, so that they acted unnaturally by buying up more goods than they needed, including hand sanitizers. This is one of the reason the price of hand sanitizers has increased. That’s why people need to know how to make hand sanitizers so that they are no longer worry about the scarcity of hand sanitizers and can make their own hand sanitizers. The purpose of this activity is to meet the needs of residents for hand sanitizer as an antiseptic with WHO standards. The method used in training in making hand sanitizers in a direct demonstration. Demonstrations are also supported by a PowerPoint and video that is displayed repeatedly with the help of an LCD projector. This was done to repeat the key points of the demonstration sp that a good level of understanding was achieved by the citizens. Then the Citizen representativeswere asked to repeat the demonstration so that the activities of seeingm listening and practicing the knowledge  of making hand sanitizers were completed. Then at the end of the event, an evaluation was carried out using the discussion or question and answer method. The results of the training in making hand sanitizers have helped meet the needs of residents for hand sanitizers, helped residents to be more independent in meeting their needs for hand sanitizers, and increased knowledge and skills of residents regarding making hand sanitizers as WHO standard antiseptics