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The Application of Barokah herb and salinity on the growth of Sangkuriang catfish Clarias gariepinus Burchell, 1822 Faulana, Arum Fahmi; Rohmah, Zuliyati
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.258-265

Abstract

The demand and need for catfish is increasing along with the growth of the Indonesian population, thus requiring greater catfish production. In addition, most of the brackish water areas in Indonesia have not been optimized for freshwater fish farming. Water salinity of 4 ppt plays a role in increasing fish growth, so when combined with the use of probiotics it has the potential to optimize catfish growth. This prompted a study on the Barokah herb, a herbal probiotic developed by the Mina Rukun fish group in Gunungkidul, which was combined with a water salinity of 4 parts per thousand (ppt) for more optimal rearing of Sangkuriang catfish. This study used a Completely Randomized Design with four treatment groups: control (K), salinity 4 ppt (S), herbal probiotics Barokah Herb with a dose of 30 mL/kg feed (P), and a combination of Barokah herb and salinity (PS). Each pond contained 375 catfish as replications with a stocking density of 250 fish/m3. Data analysis was carried out using the ANOVA Test and the Tukey HSD Test as a post hoc test. The results showed that the combination of Barokah herb with a dose of 30 mL/kg feed and salinity of 4 ppt gave the best effect on the growth of Sangkuriang catfish, feed conversion ratio (FCR) of 1.001 and specific growth ratio (SGR) of 1.021. Histo-morphologically, the combination of Barokah Herb and 4 ppt salinity showed an increase in the dimensions of the villi, crypts, and tunica mucosa in the intestine and increased the dimensions skeletal muscle fascicles of the Sangkuriang catfish. This is related to the environment that is isoosmotic to the catfish's body and also the improvement of the catfish's digestive system. Keywords: catfish growth, histo-morphology, barokah herb, salinity ABSTRAK Permintaan dan kebutuhan lele semakin meningkat seiring dengan pertumbuhan penduduk Indonesia, sehingga membutuhkan produksi lele yang lebih besar. Selain itu, sebagian besar wilayah perairan payau di Indonesia belum dioptimalkan untuk budidaya ikan air tawar. Salinitas air 4 ppt berperan dalam meningkatkan pertumbuhan ikan, sehingga ketika dikombinasikan dengan probiotik, berpotensi menjadikan pertumbuhan lele lebih optimal. Hal ini mendorong dilakukannya penelitian tentang probiotik herbal Ramuan Barokah yang dikembangkan oleh kelompok ikan Mina Rukun Gunungkidul yang dikombinasikan dengan salinitas air 4 part per thousand (ppt) agar pemeliharaan ikan lele Sangkuriang lebih optimal. Penelitian ini menggunakan Rancangan Acak Lengkap dengan empat kelompok perlakuan yaitu kontrol (K), salinitas 4 ppt (S), probiotik herbal Ramuan Barokah dengan dosis 30 mL/kg pakan (P), dan kombinasi probiotik hebal dan salinitas (PS). Setiap kolam diisi 375 ekor ikan lele sebagai ulangan dengan padat tebar 250 ekor/m3. Analisis data dilakukan dengan menggunakan Uji ANOVA dan Uji Tukey HSD sebagai uji post hoc. Hasil penelitian menunjukkan bahwa kombinasi Ramuan Barokah dengan dosis 30 mL/kg pakan dan salinitas 4 ppt memberikan pengaruh terbaik terhadap pertumbuhan ikan lele Sangkuriang, rasio konversi pakan (FCR) sebesar 1,001 dan rasio pertumbuhan spesifik (SGR) sebesar 1,021. Secara histomorfologi, kombinasi Ramuan Barokah dan salinitas 4 ppt menunjukkan adanya peningkatan dimensi vili, kripta, dan tunika mukosa pada usus serta peningkatan dimensi fasikulus otot rangka ikan lele Sangkuriang. Hal ini berkaitan dengan lingkungan yang isoosmotik terhadap tubuh ikan lele dan juga perbaikan sistem pencernaan lele. Kata kunci: histo-morfologi, pertumbuhan lele, ramuan barokah, salinitas
Review: Aplikasi Histopatologi Untuk Praktik Forensik: Review: Applications of Histopathology for Forensic Practice Faulana, Arum Fahmi; Empra, Desi Eka Putri; Rahmadian, Desti; Shalihah, Fianicha; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry Tri Sakti SG; Rohmah, Zuliyati; Septriani, Nur Indah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.6582

Abstract

Forensics plays an important role in investigating crimes because it can reveal everything related to a murder case, including the time and cause of the murder. Histopathology is the study of damage to body tissues and organs which can be used to help investigate crimes. The aim of this literature review is to study the role of histopathology applications for forensic practice. The method used is searching journals and books using Google Scholar, ScienceDirect, NCBI, and PubMed with the keywords histopathology, forensics, diagnosis, death, microscopy, disease, staining, and autopsy. The histology approach helps the pathologist make a clear determination on the cause of death. The aim of histopathology is to perfect the macroscopic diagnosis and act as an additional examination in forensic case management. One of the histopathology methods in the forensic field is the paraffin method with hematoxylin eosin staining and special stains, immunohistochemistry, and MITS. The parameters observed with this method are measurements of pulmonary fat embolism; determining the age of myocardial infarction; assessment of liver steatosis, fibrosis, and cirrhosis; determination of the age of thrombus or thromboembolism; and diagnosis of shock with various diseases. The prospect of forensic histopathology is to complement molecular diagnostic methods, so that the combination of molecular and histology methods can play a maximum role in the field of forensic pathology. The conclusion of this literature review is that histopathology can be used to confirm the diagnosis in forensic cases and has good future prospects if combined with other methods such as molecular.