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PERKEMBANGAN OVARIUM IKAN WADER PARI (Rasbora lateristriata Bleeker, 1854): PENDEKATAN HISTOLOGI Zulfadhli Zulfadhli; Nastiti Wijayanti; Bambang Retnoaji
JURNAL PERIKANAN TROPIS Vol 3, No 1 (2016)
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.612 KB) | DOI: 10.35308/jpt.v3i1.34

Abstract

This study was to determinte of development ovarian wader pari fish (Rasbora lateristriata) as part of the process of reproduction, which is the basic for information to R. lateristriata management. This study was done in october 2014 - april 2015 at Histology and Embryology Animal Laboratory of Gadjah Mada University. The object of the research was larva R. lateristriata with average weight of 0.02 grams. They were kept during 3 months. The result data of histology were analized and observed discussed descriptively. The results of observation showed that ovarian R. lateristriata indicated asincronous development patterns. The development of ovarian in the 1st month was at the chromatin nucleolar phase, while perinucleolar phase appeared in the 2nd and 3rd month.
PENGAMATAN BIOAKUSTIK PERGERAKAN IKAN SIDAT Anguilla sp. DALAM KONDISI TERKONTROL (AKUARIUM) Sri Pujiyati; Bambang Retnoaji; Ariel Hananya; M. Zainuddin Lubis
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 2 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.365 KB) | DOI: 10.29244/jitkt.v10i2.21276

Abstract

Pergerakan Ikan sidat menghasilkan suara yang disebut suara hidrodinamik yang merupakan suara atau getaran yang dihasilkan dari sebuah perubahan di medium karena pergerakan ikan. Intensitas suara hidrodinamik terjadi saat arah dan kecepatan renang ikan berubah secara cepat. Pembelokan posisi kepala akan menyebabkan perpindahan medium yang kuat.  Perpindahan tersebut menyebabkan perubahan gelombang tekanan pada medium, yang dapat terdeteksi sebagai suara oleh hydrophone. Penelitian ini bertujuan untuk menganalisis perbedaan suara ikan sidat fase elver dan fase yellow dalam kondisi terkontrol. Penelitian dilakukan di Laboratorium histologi dan embriologi Fakultas Biologi, Universitas Gadjah Mada. Waktu penelitiannya adalah 4-7 Agustus 2016. Alat yang digunakan dalam penelitian ini adalah aquarium kaca, headphone, hidrophone, Seaphone, baterai alkaline kotak, laptop. Bahan yang digunakan masing-masing 5 ekor sidat fase yellow dan fase elver.  Hasil penelitian menunjukkan bahwa Aktifitas ikan sidat fase elver dan fase yellow sangat beragam ditinjau dari  durasi suara ikan sidat saat pagi,siang dan malam.  Pola suara yang terekam berkisar dari frekuensi dari 0 sampai 22.03 KHz. Frekuensi suara ikan sidat dominan dikisaran 151 - 450 Hz, dan memiliki pola suara yang berbeda-beda untuk pagi, siang dan sore. Kesimpulan menunjukkan bahwa durasi dan pola suara hidrodinamika ikan  sidat fase elver maupun fase yellow berbeda nyata antara pagi - siang, juga siang – sore, namun untuk pagi - sore tidak berbeda nyata.
Effects of Metaldehyde and Niclosamide on the Behavior and Reproductive Capacity of Radix Quadrasi Von Moellendorf (Basommatophora: Lymnaeidae) Bambang Retnoaji
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12230

Abstract

The effects of molluscicides exposure, metaldehyde and niclosamide, on the behavior and reproductive capacity of Radix quadrasi was studied under laboratory conditions. R quadrasi were exposed to different concentration-rates of niclosamide: 0, 0.045, 0.058 and 0.075 mg/L, and of metaldehyde: 0, 100, 112 and 130 mg/L. The symptoms of poisoning of treated snails were observed closely throughout the duration of the experiment. Observations were made three times: at the beginning of treatment (0 hour), 12 hours, and 24 hours after exposure. The surviving R. quadrasi were maintained for one month to determine their reproductive capacity. Results indicated that there was immediate reaction of the snails to molluscicide exposure. Lower concentration-rates of molluscicide triggered water-leaving behavior. Such behavior occurred in all of the low and medium concentration-rates of niclosamide and metaldehyde, as well as in some snails from the control group. By contrast, higher concentration-rates caused almost instantaneous immobility. Niclosamide and metaldehyde also significantly reduced the reproductive capacity of R. quadrasi. The number of egg masses and eggs laid varied with the treatments. However, the incubation period of the treated eggs was not statistically different from the treated ones. This study showed that both niclosamide and metaldehyde significantly affected the behavior and reproductive capacity of the R. quadrasi. Between the two molluscicides used in this experiment, niclosamide was more toxic than metaldehyde.
The Effect of Ethanolic Extract of Cashew Fruit Peel on The Liver Histological Structure in Rat (Rattus norvegicus Berkenhout, 1769) Laili Mufli Zusrina; Bambang Retnoaji; Nastiti Wijayanti
Journal of Tropical Biodiversity and Biotechnology Vol 4, No 3 (2019): Desember
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.39275

Abstract

Cashew fruit peel is a waste produced from the cashew nut industry, and it has not been utilized optimally yet. Cashew peel extract has the potential to be used as a contraceptive agent, which capable of reducing reproductive capacity. However, its side effects on other tissue and organ such as liver not clearly studied yet. This study aims to determine the effect of ethanolic extracts of cashew peel on the histological structure of the white rat liver. In this study, 21 female white rats were used and be grouped for control (6 mice) which were treated with CMCMa 0.5% and 15 mice were treated with peel extract of 500 mg/kg body every day for one month. Liver for examination was collected sequentially at 3rd, 5th, 8th, 11th, and 14th of the estrous cycle. The liver was processed for histological observation and stained with Hematoxylin Eosin and Mallory Acid Fuchsin staining solution. The liver hepatocyte was observed for it abnormality and be scored to calculate the number of cell damage or abnormality. The result showed that peel extract-treated mouse liver was similar to control ones; we did not witness any evidence of fibrosis, pyknosis and cellular necrosis on either control or treated mouse. Statistical analysis by SPSS showed that the p-value between the control and treatment groups was 0.078 (> 0.05) so there was no significant difference between control and treatment. It could be concluded that ethanolic extracts of cashew nuts peel with a concentration of 500 mg/kg body weight caused no effect on the mouse liver histological structure. application with reduced-dosages of NPK fertilizers were arranged in a random block design with three replicates. The results show that large quantities of silica bodies attached to the surface of EFB fibers and amounting to 0.44% soluble Si. The FFB data indicated that the application of 75% NPK + 500 kg composted EFB + 2 L BioSilAc/ha/year on a five-year-old plant resulted in higher yield than that obtained from 100% standard dosage of NPK. The study also revealed that the application of EFB compost reduced 50% of BioSilAc dosage.
Pengaruh Insektisida Klorpirifos Terhadap Struktur Histologi Intestinum Ikan Wader Pari (Rasbora lateristriata Bleeker, 1854) Jerizqa Ayu Fajar Nita; Bambang Retnoaji
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 12 No. 1 (2022): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.12.1.1-11

Abstract

Chlorpyrifos is one of commonly used insecticides. However, the use of insecticides in agriculture has negative impacts including lethal effect to non-target organisms. Wader pari fish is a type of freshwater fish that lives in rice fields or rivers and can be used as an indicator of water pollution. The purpose of this study was to determine the intestinal histological structure, the histopathological effect of chlorpyrifos on intestine, and population number of goblet cells, respectively. The treatment concentration of 0.001; 0.005; and 0.01 ppm were determined by preliminary trial and exposed for 168 hours. Fish behavior and water quality were monitored. The fish were still alive until the last day of exposure were then made intestinal histological preparations using the paraffin method and staining with Haematoxylin Eosin (HE), Mallory Acid Fuchsin (MAF), Periodic Acid Schiff (PAS). The results showed that the histological structure of the wader pari intestine consisted of mucosal tunica, submucosal tunica, muscularis tunica, and serous tunica. Furthermore, chlorpyrifos has a significant effect on population number of goblet cells. The intestine damages were edema, vacuolization, cloudy swelling, hemorrhage, lysis, and fusion of vili. It could be concluded that chlorpyrifos caused intestinal damage in wader pari fish.
Life-stage dependent histological characterization of liver HIF-1α expression in the Indonesian shortfin eel (Anguilla bicolor bicolor McClelland, 1884) Nyan Hayu Widiastuti; Bambang Retnoaji
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20223

Abstract

The Indonesian shortfin eel (Anguilla bicolor bicolor McClelland, 1884) is catadromous fish. The developmental phase of the fish is influenced by the availability and type of feed, and dissolved oxygen levels (DO). Oxygen level fluctuations during migration from sea to fresh waters can cause hypoxia and trigger activation of the Hypoxia-inducible factor 1 alpha gene (HIF-1α). Therefore, undertanding HIF-1α expression is very important in understanding the life cycle and physiology of this organism. This study aimed to perform a histological comparison between the liver structure of eel in the elver eel phase to silver eel and compared the expression of HIF-1α in the liver. The eels were collected from Kulon Progo Regency, Segara Anakan in Cilacap, and Cangkringan in Sleman, respectively. The eel livers were prepared with standard paraffin method. The samples were stained with Hematoxylin-Eosin, Mallory Acid Fuchsin and Periodic Acid-Schiff Alcian Blue for histological observation. The HIF-1α protein expression was detected using immunohistochemistry. The results showed that the structure of liver elver, yellow, and silver eel shared similar tubular hepatocytes. Moreover, there was no significant differences in the parenchymal structure of liver of all stage of eel. Mucin levels were different in each life cycle phase. HIF-1α expression varied by eel phase, with the elver eel showing weak expression, yellow stage showing moderate expression, and silver eels displaying the highest levels of expression. We conclude that the highest expression of HIF-1α was found in the silver eel, which resides in freshwater environments and is found in freshwater while returning to the sea for reproduction.
Histological Study of Phyllidia coelestis (Nudibranch) Epidermal Tissue from Pasir Putih, Situbondo Karunia Galih Permadani; Bambang Retnoaji
Indonesian Journal of Biology Education Vol 1, No 1 (2018): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.81 KB) | DOI: 10.31002/ijobe.v1i1.1047

Abstract

Nudibranch are a mollusk which can be found in all zones of ocean. Further, they are distributed widely from subtropical to tropical areas. The Nudibranch is characterized by lack of a shell and usually have a bright shiny color. Nudibranch has a potency to be used in the medical field because they have chemical compound inside their body. These chemical compound are the result of secondary metabolites from symbiosis with zooxanthellae in nudibranch tissues.  This research aims to study the structure of epidermal tissue of the Phyllidia coelestis collected from Pasir Putih, Situbondo, East Java Indonesia. The results showed that the epithelial structure of the Phyllidia coelestis composed of cuboidal epithelium with vacuoles spread regularly along the mantle layer below the epidermal layer. Gland cells are rarely found in the outer epithelial layer. The foot epidermal layer consisted of simple ciliated columnar epithelial cells, with goblet-shaped mucous secreting glands among the epithelium. Gill tissue structure was composed of cell layers of cuboidal ciliated epithelial cells resting on a basement membrane and connective tissues
Indeks Gonadosomatik dan Struktur Histologis Gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846) Luthfi Nurhidayat; Febrina Nanda Arfiani; Bambang Retnoaji
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.456

Abstract

Uceng fish is wild native fish of Indonesia that live in fresh water river. It is very common that people consume the fish for it high protein contain. High demand of fish availability cause the fish exploitation increasing rapidly in nature. The high intensity of fish exploitation, soon will induce declining population of the fish and leading to extinction. Uceng fish cultivation is one alternative to solve the tread. The purpose of this research is to examine the Gonadosomatic Index (GSI) and gonad histological structure of male and female uceng fish (Nemacheilus fasciatus) on the period of immature and mature. Fish were dissected , weighed and the gonads were measured for gonadosomatic index. Moreover, gonad were fixed in NBF 5% for histological preparation by paraffin method and which than were stained by Hematoxylin-Eosin. The result were analyzed for both qualitatively and quantitatively. Qualitative analysis was conducted for gonad histological structure of male and female fish, while quantitative analysis was for Gonadosomatic Index (GSI) of fish respectively. The result showed that there were differences on the gonadosomatic index of males and females fish, the histological structure of ovarian follicle and the composition of the spermatogenic cells of immature gonads and mature gonads of the fish respectively. It was conclude that there are differences on Gonadosomatic Index and gonad histological structure on immature and mature of male and female of Uceng fish (N. fasciatus).
Struktur Histologi Insang pada Combtooth blennies (Blenniidae) di Pantai Krakal, Gunung Kidul, Yogyakarta Muhammad Zuchri Zayzda; Bambang Retnoaji
Jurnal Perikanan Universitas Gadjah Mada Vol 23, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.65832

Abstract

Blenniidae adalah famili ikan yang ditemukan di ekosistem pesisir seperti zona intertidal. Gunung Kidul memiliki banyak pantai kars dengan kesamaan dalam kondisi dan formasi geologi. Keanekagaman dan histologi Blenniidae di Gunung Kidul, terutama di Pantai Krakal, masih belum banyak dipelajari. Penelitian ini bertujuan untuk mengetahui morfometri serta struktur histologi respirasi anggota famili Blenniidae yang ditemukan di pantai Krakal. Penelitian ini dilakukan di pantai Krakal dengan metode pengambilan sampel bebas. Sampel kemudian dibawa ke Laboratorium Struktur Perkembangan Hewan untuk diukur dan dibedah. Sebanyak 3 spesies dengan masing-masing 5 sampel ditemukan di zona intertidal pantai Krakal. Dari pengamatan morfometrik, ditemukan bahwa Istiblennius edentulus dengan Istiblennius dussumeri memiliki rata-rata rasio morfometri yang berbeda sementara Istiblennius lineatus memiliki rata-rata rasio morfometri yang sama dengan Istiblennius  edentulus dan Istiblennius dussumeri. Hasil pengamatan struktur histologis insang tidak menunjukan adanya perbedaan jaringan penyusun insang antar spesies ikan, yang tersusun oleh lamella primer (1), lamella sekunder (2), sel eritrosit (3), sel pilar (4), sel epitel (5), sel mukus (6), dan sel klorit (7). Struktur insang tiap spesies ikan ini sesuai dengan kondisi habitat berupa udara terbuka sehingga dapat memperluas area respirasi dan menyimpan air di dalam insang.
PENGARUH BAHAN AKTIF INSEKTISIDA CHLORPYRIFOS TERHADAP STRUKTUR HISTOLOGIS HEPAR IKAN WADER PARI (Rasbora lateristriata Bleeker, 1854) Khoiruddin Anshori; Bambang Retnoaji
BERITA BIOLOGI Vol 21, No 1 (2022)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i1.4127

Abstract

The use of insecticides of the organophosphorus group is increasing along with the expansion of agricultural land. Chlorpyrifos (CPF) as one of the active ingredients can accumulate in the fish and impact human health through bioaccumulation and biomagnification mechanisms. This research aims to investigate the effects of sub-lethal exposure of CPF to yellow rasbora (Rasbora lateristriata) using mortality parameters and histological changes in the liver. One-month-old yellow rasbora larvae were exposed to CPF with concentrations of 0.001, 0.005, and 0.01 ppm for 168 hours based on the preliminary test. The paraffin method was used in the manufacture of liver histological preparations with Haematoxylin-Eosin staining. Observations were conducted descriptively-quantitatively to determine the type and extent of damage to the liver. The actual test results showed that the LC50 value of CPF against yellow wader for 168 hours was 0.078 ppm. The higher the concentration used shows a decrease in the survival rate of fish. Histological observations showed cell swelling, necrosis, and haemorrhagic tissue damage in CPF treatment. The increase in CPF concentration significantly affected cellular damage to hepatocyte cells, with the highest rate of damage at a concentration of 0.01 ppm. 
Co-Authors Aeniah, Siti Allimi, Hayu Swari Andi Gita Maulidyah Indraswari Suhri Anita Restu Puji Raharjeng Apriliyani, Tia Ardaning Nuriliani Arief Maulana Sabilillah Ariel Hananya Ariesti, Wiwin Arum Fahmi Faulana Ascarti Adaninggar Della Blatama Desi Eka Putri Empra Desti Rahmadian Devi Anisa Suci Empra, Desi Eka Putri Fajar Sofyantoro Febrina Nanda Arfiani Fianicha Shalihah Halim, Shuha Ma’muriyah Hayu Swari Alimi Hendry Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti SG Saragih Hendry Tri Sakti SG Saragih Jerizqa Ayu Fajar Nita Jessy Octavia Harahap Joko Nugroho Wahyu Karyadi Karlina, Ina Karunia Galih Permadani Khasanah, Luthfia Uswatun Khoiruddin Anshori Khoiruddin Anshori Laili Mufli Zusrina Laili Mufli Zusrina Larasshita Putri Adhitya Sani Lathief Al Umami M. Zainuddin Lubis Maharesi, Chesa Ekani Muhammad Zuchri Zayzda Murtiadi Erlan Supraitno Mustamin, Yusdar Muthia Arrusyda Nastiti Wijayanti Nastiti Wijayanti Nurhidayat, Luthfi Nyan Hayu Widiastuti Pradnya Paramita Pusparini, Nur Ainun Oktavia Rey, Juniman Ria Vena Septhay Sari Rohmah, Zuliyati Ruth Liananda Citra Dolok Saribu Saeed, Faisal Sandi Francisco Pratama Saragih, Hendry Sari, Ria Vinola Septhya Saribu, Ruth Liananda Citra Dolok Septriani, Nur Indah Septriani, Nur Indah Sih Kahono Simanungkalit, Eben Ezer Slamet Widiyanto Sri Pujiyati Supraitno, Murtiadi Erlan Susilo Hadi, Susilo Tia Apriliyani Yunita, Neri Zulfadhli Zulfadhli Zuliyati Rohmah Zuliyati Rohmah Zusrina, Laili Mufli