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All Journal Indonesian Journal of Tropical and Infectious Disease
Sin War Naw
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Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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Screening of Legionella pneumophila from Well Water in Magetan Regency, Indonesia Hapsari, Nafisah Nurul; Kurniawan, Muhammad Ridho Hafid; Sucipto, Teguh Hari; Fajar, Nur Syamsiatul; Herupradopo, Eduardus Bimo Aksono; Fauziyah, Shifa; Widya, Alicia Margaretta; Aquaresta, Febriana; Sin War Naw
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 3 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i3.48755

Abstract

Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp).
Co-Authors Aquaresta, Febriana Hapsari, Nafisah Nurul Herupradopo, Eduardus Bimo Aksono Kurniawan, Muhammad Ridho Hafid Nur Syamsiatul Fajar, Nur Syamsiatul Shifa Fauziyah Teguh Hari Sucipto, Teguh Hari Widya, Alicia Margaretta