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All Journal Indonesian Journal of Tropical and Infectious Disease Jurnal Penelitian Pendidikan IPA (JPPIPA) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Nur Syamsiatul Fajar, Nur Syamsiatul
Tropical Disease Diagnostic Center (TDDC) – Institute of Tropical Disease, Universitas Airlangga.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Matondang, Muhammad Qushai Y.; Nasronudin, Nasronudin; Aksono, Eduardus Bimo; Lusida, Maria Inge; Nastri, Aldise Mareta; Fajar, Nur Syamsiatul; Jannah, Lilis Mundri
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.4 KB)

Abstract

Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1st RT-PCR and 2nd Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.
QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Matondang, Muhammad Qushai Y.; Nasronudin, Nasronudin; Aksono, Eduardus Bimo; Lusida, Maria Inge; Nastri, Aldise Mareta; Fajar, Nur Syamsiatul; Jannah, Lilis Mundri
Indonesian Journal of Tropical and Infectious Disease Vol. 4 No. 4 (2013)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.4 KB) | DOI: 10.20473/ijtid.v4i4.232

Abstract

Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1st RT-PCR and 2nd Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.
Screening of Legionella pneumophila from Well Water in Magetan Regency, Indonesia Hapsari, Nafisah Nurul; Kurniawan, Muhammad Ridho Hafid; Sucipto, Teguh Hari; Fajar, Nur Syamsiatul; Herupradopo, Eduardus Bimo Aksono; Fauziyah, Shifa; Widya, Alicia Margaretta; Aquaresta, Febriana; Sin War Naw
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 3 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i3.48755

Abstract

Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp).
Expression of the curculin gene from different organs of Curculigo latifolia under different culture conditions Muslihatin, Wirdhatul; Fajar, Nur Syamsiatul; Wibowo, Anjar Tri; Manuhara, Yosephine Sri Wulan
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1131

Abstract

Curculigo latifolia is the main source of curculin. Curculin is a special kind of protein that tastes sweet and can change the way food tastes, and is naturally expressed in fruit. This study aims to measure and compare the relative expression of the curculin gene in different organs, i.e., fruit and leaf, under in vitro and in vivo conditions. In this study, mRNA isolation was carried out in tissues derived from fruits and leaves grown in vitro and in vivo (in the soil) of C. latifolia from West Java, Indonesia. Leaves from 20 weeks seedling on polybag, fruit 40 days after anthesis, and leaves from 20 weeks seedling of C. latifolia cultured on MS0 medium that were used in this experiment. The relative expression was measured using qRT-PCR. The results showed that the comparison of curculigo’s parts in the leaves was lower than in the fruit. The lowest expression was obtained in leaves grown under in vitro conditions at 0.001-fold, while leaves grown in the soil at 0.566-fold compared to curculin gene expression in fruit. This study concludes that the expression of the Curculin gene in fruit is different between the fruit and leaves, and the growth conditions have an influence on Curculin gene expression, where Curculin gene expression under in vitro conditions is lower than expression under in vivo conditions. The curculin is not only expressed in the fruit but also the leaves, so it has the potential to be developed.
The Impact of Smoking on Sperm Quality and the DAZ Gene Sukarjati; Choirunnisa, Nanis; Fajar, Nur Syamsiatul
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.9213

Abstract

Indonesia's male population is mostly smokers. The chemical content in cigarettes can reduce the quality of spermatogenesis, where spermatogenesis is influenced by the DAZ gene (deleted in Azoospermia). This study aims to analyze sperm quality in light, moderate, and heavy smokers in 3 age groups and analyze the presence of the DAZ gene in heavy smokers. This study used 180 samples divided into three age groups, namely men aged 26-30 years, 31-35 years, and 36-40 years. Each age group recorded the number of cigarettes consumed per day (light, moderate, and heavy smokers) and examined the quality of sperm. While the DAZ gene analysis used 10 samples of heavy male smokers, aged 25-40 years. Sperm quality analysis was based on the WHO laboratory manual for human semen testing, while DAZ gene analysis used the PCR method. Sperm quality data were analyzed using Kruskal-Wallis, while the DAZ gene was analyzed qualitatively descriptively by analyzing electrophoresis photos. The results of this study can be concluded that daily cigarette consumption in the age group affects sperm quality and in heavy smokers, there are 10% who experience deletions in the DAZ gene. Thus cigarettes can reduce spermatozoa quality.
Co-Authors Aksono HP., Eduardus Bimo Aldise Mareta Nastri, Aldise Mareta Anjar Tri Wibowo Aquaresta, Febriana Choirunnisa, Nanis Hapsari, Nafisah Nurul Kurniawan, Muhammad Ridho Hafid Lilis Mundri Jannah, Lilis Mundri Maria Inge Lusida Muhammad Qushai Y. Matondang, Muhammad Qushai Y. Nasronudin Nasronudin Shifa Fauziyah Sin War Naw Sukarjati Sukarjati, Sukarjati Teguh Hari Sucipto, Teguh Hari Widya, Alicia Margaretta Yosephine Sri Wulan Manuhara