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All Journal Indonesian Journal of Tropical and Infectious Disease
Widya, Alicia Margaretta
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Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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LOW CD4 LYMPHOCYTE COUNT RELATED RISK TO Pneumocystis jiroveci PNEUMONIA IN HIV/AIDS PATIENTS FROM BRONCHOALVEOLAR LAVAGE SPECIMENS USING REAL TIME PCR DETECTION Widya, Alicia Margaretta; Mertaniasih, Ni Made; Kawilarang, Arthur Pohan; Marhana, Isnin Anang
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.955 KB) | DOI: 10.20473/ijtid.v6i6.6309

Abstract

HIV and opportunistic infections remain a big problem especially in developing country. Pneumocystis jiroveci pneumonia is a prevalent infection  in HIV infected patient with high mortality rate. Diagnosis of Pneumocystis jiroveci pneumonia is mainly based on clinical evidence. Microbiological diagnosis is quite challenging since this microorganism cannot be cultured and is mainly based on microscopic examination. Microscopic examination with special staining is still a gold standard diagnosis for P. jiroveci infection.The objectives of this study was to describe CD4 lymphocyte profile and establish microbiological diagnosis with recent molecular method in PJP suspected HIV positive patients. Fiberoptic bronchoscopy of HIV infected patients with lower respiratory tract infection in Dr. Soetomo general hospital Surabaya were performed to collect bronchoalveolar lavage specimens  from December 2016 to April 2017 for identification of Pneumocystis jiroveci using real time PCR assay. Positive samples were then evaluated for  microscopic examination with Gommori Methenamine Silver staining for comparison. Patient's CD4 lymphocyte count were gathered prior of admission. CD4 lymphocyte count from this study were very low with 61% of the patients were below 50 cells/ µL. There were five of  total thirteen patients (38,5%) with positive real time PCR assay (MSG gene)  and one patient was also positive with GMS staining showing characteristic cysts shape with dark centered area of P. jiroveci. Patient with positive microscopic examination showed no history of prophylactic therapy. Low CD4 lymphocyte count remains a strong risk factor of P. jiroveci pneumonia in HIV/AIDS patients. Real time PCR assay shows high value in detection of P. jiroveci regarding patient's prophylactic status.
Screening of Legionella pneumophila from Well Water in Magetan Regency, Indonesia Hapsari, Nafisah Nurul; Kurniawan, Muhammad Ridho Hafid; Sucipto, Teguh Hari; Fajar, Nur Syamsiatul; Herupradopo, Eduardus Bimo Aksono; Fauziyah, Shifa; Widya, Alicia Margaretta; Aquaresta, Febriana; Sin War Naw
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 3 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i3.48755

Abstract

Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp).
Co-Authors Aksono HP., Eduardus Bimo Aquaresta, Febriana Hapsari, Nafisah Nurul Isnin Anang Marhana Kawilarang, Arthur Pohan Kurniawan, Muhammad Ridho Hafid Ni Made Mertaniasih Nur Syamsiatul Fajar, Nur Syamsiatul Shifa Fauziyah Sin War Naw Teguh Hari Sucipto, Teguh Hari