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All Journal PELAGICUS: Jurnal IPTEK Terapan Perikanan dan Kelautan Jurnal Bluefin Fisheries Jurnal Penelitian Chanos Chanos Proceedings of the Vocational Seminar on Marine and Inland Fisheries
Yudana, IGP Gede Rumayasa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Mechanical Engineering

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KORELASI POPULASI VIBRIO TERHADAP FAKTOR LINGKUNGAN PADA KOLAM PEMELIHARAAN LARVA UDANG VANNAMEI (Litopenaeus vannamei) DI SITUBONDO, INDONESIA Puspitasari, Indah; Mulyasari, Cholifah Desi; Yudana, IGP Gede Rumayasa
Chanos Chanos Vol 18, No 2 (2020): Chanos chanos
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.464 KB) | DOI: 10.15578/chanos.v18i2.9549

Abstract

Vibrio merupakan bakteri yang menyebabkan banyak penyakit pada budidaya udang. Monitoring kelimpahan Vibrio penting dilakukan sebagai tindakan pencegahan penyakit pada larva udang vannamei.. Perbedaan sistem manajemen kualitas air pada setiap kolam budidaya dapat berpengaruh kepada dinamika populasi Vibrio. Studi mengenai korelasi populasi Vibrio dengan faktor lingkungan pada tambak pemeliharaan larva udang vannamei telah dilakukan pada bulan Maret – Juni tahun 2018 di Situbondo, Jawa Timur Indonesia. Populasi Vibrio naik secara gradual seiring dengan pertumbuhan larva. Pada stadia larva PL6 mengalami penurunan saat pemberian probiotik kemudian naik kembali pada PL7 sampai dengan PL12. Total Vibrio memiliki korelasi positif yang signifikan (Spearman Correlation Test, P<0,05) dengan parameter kualitas air; NH4 (r=0,852), NH3 (r=0,679), Salinitas (r=0,565), dan NO2 (r= 0,453). Namun memiliki korelasi negative yang signifikan dengan pH (r=-0,478, P<0,05) dan koreasi tidak signifikan dengan Suhu (r=-0,02, P>0,05). Dari 20 sampel air terhitung jumlah rata-rata Vibrio pada media pembenihan adalah  0,15 CFU/mL, masih dalam rentang normal dalam kegiatan pembenihan udang vanname.
Pengaruh Parameter Kimia Terhadap Parameter Biologi Pada Kualitas Air Kolam Pulokerto, Kabupaten Pasuruan Yudana, IGP Gede Rumayasa; jayanti, shara
JURNAL BLUEFIN FISHERIES Vol 5, No 2 (2023)
Publisher : Politeknik Kelautan dan Perikanan Bitung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The bad water quality is caused by many people managing waste poorly, which can pollute the environment and groundwater sources and create a negative impact on the balance of the ecosystem and surrounding waters. The quality of the water used as a cultivation medium in both columns and ponds is very important to know. This research was conducted to determine the effect of chemical parameters and biological parameters on water quality in the Pulokerto pond, Pasuruan Regency. Water quality measurements were carried out in 3 places, namely plot 13, plot 14 and SB. The chemical parameters tested were nitrite, phosphate, ammonium, Ca, Mg, TOM and alkalinity based on total (CO32- and HCO3-). The biological parameters tested were plankton abundance, TBC (Total Bacteria Count) and TVC (Total Vibrio Count). The results of water quality testing in plot 13 contained high levels of Blue Green Algae at DOC 34, namely 29 cells/ml and in plot SB at DOC 34 it was 22.9 cells/ml and at DOC 59 it was 17.26 cells/ml. Based on the results of the analysis of the quality values of chemical and biological parameters, it can be concluded that chemical parameters do not really influence the biological parameters of water quality in the Pulokerto pond, but rather the results of chemical water quality parameters such as nitrite content, hardness (Ca and Mg) and TOM of P.13 at the time of The 34th Shrimp DOC exceeds the 2014 SNI standard, causing an increase in the number of Blue Green Algae (BGA) which has toxic effects. The number of BGAs exceeded the standard number, namely 29 cells/ml, and on P.13, the 58th DOC showed that the results of green colonies on TVC (Total Vibrio Count) exceeded the standard, namely 200 cells/ml. Green vibrio colonies are a characteristic of the Vibrio parahaemolyticus species which has a poison that is dangerous for fish and shrimp. 
Compliance Evaluation of The GMP and SSOP Implementation of Frozen Tuna Loin Processing in PT. XYZ, Bitung Hariyoto, Fitroh Dwi; Ticoalu, Fidel; Yudana, IGP Gede Rumayasa; Maringka, Gabriela Stela
PELAGICUS Volume 5 Nomor 2 Tahun 2024 (Article in progress)
Publisher : Politeknik Kelautan dan Perikanan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/plgc.v5i2.14989

Abstract

The implementation of basic feasibility programs such as Good Manufacturing Practices (GMP) and Sanitation Standard Operating Procedures (SSOP) must be carried out properly to maintain and ensure the feasibility and food safety of processed fishery products in the fisheries industry sector as one of the main components of the Indonesian economy. Bitung City as the center of fisheries industry in Indonesia has a variety of Fish Processing Units (UPI) that produce various processed products, such as frozen tuna loin as a primer commodity. This research, which was conducted from February to May 2023 through direct observation of the implementation of GMP and SSOP, aims to assess the suitability of GMP and SSOP implementation in the frozen tuna loin production process. Qualitative, quantitative, and descriptive comparative analysis were conducted based on the Regulation number 75/M-IND/PER/7/2010 from the Minister of Industry for the analysis of GMP implementation and the Regulation number PER.19/MEN/2010 from the Minister of Marine and Fisheries Affairs on SSOP conformity. The results obtained during the study showed that the percentage of GMP implementation conformity was 83% of the 200 items assessed. Meanwhile, of the 28 SSOP items that have been applied to frozen tuna loin processing, the suitability of the application reached 78.57% and is running well, although there are some minor errors. Compliance evaluation needs to be carried out in the form of improvements to 34 non-conformities in GMP and 6 improvements in SSOP implementation to improve the safety of frozen tuna loin products.
EFEKTIVITAS IMUNOSTIMULAN EKSTRAK Gracillaria sp. TERHADAP BAKTERI Aeromonas salmonicida SECARA IN VIVO Jayanti, Shara; Asmarany, Anja; Yudana, IGP Gede Rumayasa
Chanos Chanos Vol 23, No 2 (2025): CHANOS CHANOS
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/chanos.v23i2.15474

Abstract

This study was determining the effect of immunostimulant from Gracillaria sp. on Aeromonas salmonicida bacteria in vivo. The parameters were the survival rate, total leukocytes (Cyprinus carpio) and IC50 of Gracillaria sp. as an immunostimulant for koi that infected with Aeromonas salmonicida. Gracillaria sp. extract can improve the immune system, as seen from immune parameters such as leukoocytes and health. Gracillaria sp. extract with 70% ethanol has a flavonoid content of 1.9 mg QE/g. Flavonoids are antioxidant that can increase the immune system to against pathogen. The application of immunostimulant is given orally by mixing with the cellulase enzyme as much as 7.2 mg/g then added to koi feed at a dose based on IC50 Gracillaria sp. which is 18.6 mg/ml. he study was conducted using 6 treatments where the results showed that the highest total of leukocytes was in fish fed with immunostimulants at a dose of 2IC50 and infected with Aeromonas sp (P2) of 37.12 x 104 cells/mm3. While the lowest total leukocytes were in P5 fish, which was 16.08 x 104 cells/mm3. The lowest survival rate was in P5 (Fish fed commercial feed infected with Aeromonas salmonicida) 80% and in addition the survival value of the fish was 100%. In P5 fish, the fish fins became soft, the fish scales fell out, the surface of the fish skin produced a lot of mucus and there were wrinkles on the fish's tail fins.Penelitian ini dilaksanakan untuk mengetahui pengaruh pemberian imunostimulan dari Gracillaria sp. terhadap bakteri Aeromonas salmonicida secara in vivo. Parameter yang diuji adalah tingkat kelangsungan hidup, total leukosit ikan koi (Cyprinus carpio) serta dosis efektif dari ekstrak Gracillaria sp. sebagai immunostimulan untuk ikan koi yang terimfeksi bakteri Aeromonas salmonicida. Ekstrak Gracilaria sp. cukup efektif digunakan sebagai bahan imunostimulan untuk meningkatkan sistem imun ikan, terlihat dari parameter imun seperti lekosit dan kesehatan ikan. Ekstark Gracillaria sp.dengan pelarut etanol 70 % terbukti memiliki kandungan flavonoid sebesar 1,9 mg QE/g. Flavonoid merupakan senyawa bioaktif yang dapat meningkatkan sistem imun terhadap infeksi patogen. Aplikasi imunostimulan diberikan secara per oral dengan pemberian enzim selulase sebanyak 7,2 mg/g yang ditambahkan pada pakan ikan koi dengan dosis berdasarkan IC50 Gracillaria sp. yaitu 18,6 mg/ml. Penelitian dilakukan menggunakan 6 perlakuan dimana hasil menunjukkan bahwa jumlah total sel leukosit tertinggi adalah pada ikan yang diberikan pakan berimunostimulan dengan dosis 2IC50 dan diinfeksi dengan Aeromonas sp (P2) sebesar 37,12 x 104 sel/mm3. Sedangkan total leukosit terendah pada ikan P5 yaitu sebesar sebesar 16,08 x 104 sel/mm3. Tingkat kelangsungan hidup paling rendah adalah pada P5 (Ikan diberikan pakan komersil dengan diinfeksi Aeromonas salmonicida) 80% dan selain itu nilai kelangsungan hidup ikan adalah 100%. Pada ikan P5 mengalami kelembekan sirip ikan, kerontokan sisik ikan, permukaan kulit ikan mengeluarkan lendir yang banyak serta geripis pada sirip ekor ikan.
NUTRITIONAL AND MICROBIOLOGICAL PROFILE OF FERMENTED SKIPJACK TUNA VISCERA SILAGE BASED ON MOLASSES VARIATIONS AS A CANDIDATE FOR VALUE-ADDED FEED INGREDIENTS Yudana, IGP Gede Rumayasa; Hariyoto, Fitroh Fitroh Dwi; Ondang, Hetty MAGRITTHA PAULINE; TUMANDUK, Nova MAGDALENA; WEWENGKANG, Itje ANTI; Ticoalu, Fidel JAMES BONIFACIO; akbar, mohamad; Wowiling, Fernando; Rakhmayeni, Dyah ayu; NATALIA, DESSY ATIKA; Abdillah, Amelia
Proceedings of The Vocational Seminar on Marine & Inland Fisheries Volume 3 Nomor 1 Tahun 2026
Publisher : Politeknik Kelautan dan Perikanan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/voc_seminar.v3i1.20737

Abstract

This study aims to evaluate the nutritional and microbiological profiles of fermented skipjack tuna (Katsuwonus pelamis) entrails silage based on variations in molasses addition as a candidate for value-added feed ingredients. The study was conducted from September to November 2025 at the Processing Workshop of the Bitung Marine and Fisheries Polytechnic. The skipjack tuna entrails raw material was fermented for 14 days using probiotics as a source of Lactic Acid Bacteria (LAB) with molasses added at levels of 10%, 20%, and 30%. The parameters tested included crude protein, crude fat, Total Plate Count (TPC), total LAB, and the presence of Salmonella. The results showed that increasing the molasses level tended to decrease the protein content and produced variations in crude fat values in the silage product. Microbiological activity showed that LAB was higher in the 30% molasses treatment, while TPC was relatively stable in all treatments. All samples were declared safe for because they were Salmonella-negative. Overall, fermented skipjack tuna entrails silage with a molasses level of 10% provided a more optimal nutritional and microbiological profile than other treatments, making it a potential candidate for value-added feed ingredients in supporting the sustainable utilization of fishery waste
Co-Authors Abdillah, Amelia Asmarany, Anja Fitroh Dwi Hariyoto Hariyoto, Fitroh Fitroh Dwi INDAH PUSPITASARI Jayanti, Shara Maringka, Gabriela Stela Mohamad Akbar, Mohamad Mulyasari, Cholifah Desi Natalia, Dessy Atika Ondang, Hetty Magrittha Pauline Rakhmayeni, Dyah Ayu Ticoalu, Fidel Ticoalu, Fidel JAMES BONIFACIO Tumanduk, Nova Magdalena WEWENGKANG, Itje ANTI Wowiling, Fernando