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Isolasi Cendawan yang Berperan dalam Proses Pembuatan Pliek U (Makanan Fermentasi Khas Aceh) Muhammad Asril; Analekta Tiara Perdana; Mahyarudin Mahyarudin; Anja Asmarany; Qurrota A’yun
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 1 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1019.499 KB) | DOI: 10.20884/1.mib.2019.36.1.807

Abstract

Pliek u merupakan produk residu hasil fermentasi kelapa oleh mikroorganisme tertentu secara spontan. Produk hasil fermentasi ini telah dimanfaatkan secara turun temurun oleh masyarakat Aceh sebagai bahan bumbu masak seperti pembuatan sayur, olahan ikan dan sambal buah-buahan, sedangkan minyak yang dihasilkan selama proses fermentasi dijadikan sebagai obat-obatan. Penelitian ini bertujuan untuk mengetahui jenis cendawan yang berperan sebagai fermentor alami selama proses pembuatan pliek u. Cendawan diisolasi dari substrat kelapa yang telah terfermentasi selama 5 hari dan ditumbuhkan di media Potato Dextrose Agar. Pengamatan makroskopis dilakukan dengan mengamati warna spora dan warna misellium, sedangkan pengamatan mikroskopis dilakukan dengan teknik slide culture yaitu mengamati bentuk dan ukuran spora. Hasil penelitian menunjukkan bahwa terdapat 9 isolat cendawan yang terdiri dari 6 isolat dari genus Aspergillus yaitu A. niger 1, A. niger 4.1, A. niger 5, A. flavus 3, A. flavus 4.2, A. flavus 6. Sedangkan 3 isolat lainnya merupakan Penicillium citrinum, Thielaviopsis paradoxa dan Geotrichum candidum. Keberadaan cendawan ini dipengaruhi oleh substrat yang digunakan pada fermentasi pliek u. Selain itu, juga diperoleh minyeuk simplah yang berwarna kuning dan merupakan produk samping selama proses fermentasi pliek u. Hasil ini menegaskan bahwa cendawan yang berperan dalam proses pembuatan pliek u sangat beragam tergantung proses pembuatannya.
Abundance of Phytoplankton in Vannamei Shrimp (Litopenaeus vannamei) Farming Pond at the Center for Brackish Water Aquaculture Fisheries (BBPBAP) Jepara Anja Asmarany
Journal of Aquaculture Development and Environment Vol 5, No 1 (2022): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v5i1.5678

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one of the commodities that is a priority in aquaculturedevelopment activities in Indonesia. The success of vannamei shrimp culture is influenced by variousfactors such as fry quality, feed quality, land carrying capacity, application of biosecurity and goodwater quality management. Water quality is closely related to the phytoplankton community containedin a waters. This study aims to analyze the abundance and dominance of plankton species found inpond waters. Monitoring the abundance of phytoplankton was carried out in 4 plots of vannameishrimp aquaculture ponds at the Brackish Water Aquaculture Fisheries Center (BBPBAP) Jepara,namely plots A51, A51, A61 and A62. Identification and calculation of phytoplankton cells werecarried out using a haemocytometer and Sedgwick. The results of the analysis showed that the highestabundance of phytoplankton was found in plots A61 at week 9 with a cell number of 36.73 x 106cells/L, while plots A51, A52 and A62 decreased. Overall plot A62 had better water quality conditionswhen compared to plots A51, A52 and A61 indicated by the phytoplankton abundance value of 34.63x 106 cells/L at week 9 and the dominance index of green algae, namely D > 0.5.
KELIMPAHAN BAKTERI HETEROTROF PADA TAMBAK DENGAN JENIS MANGROVE YANG BERBEDA DI PULOKERTO PASURUAN Setyastuti, Tri Ari; Puspitasari, Indah -; Sukamto, Dwi; Asmarany, Anja
Chanos Chanos Vol 18, No 1 (2020): Chanos chanos
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.667 KB) | DOI: 10.15578/chanos.v18i1.8961

Abstract

Pusat Studi Mangrove merupakan pengembangan tambak sylfofisheries dengan beberapa jenis mangrove diantaranya adalah Avicennia marina, Rhizophora apiculata, Avicennia alba dan Sonneratia alba. Penelitian ini bertujuan untuk melakukan karakterisasi bakteri pada tutupan mangrove yang berbeda. Serasah yang berlebihan dapat mengakibatkan menumpuknya bahan organik dan meningkatkan derajat keasaman perairan pada tambak silvofishery.  Namun keberadaan bakteri heterotroph yang mampu menguraikan bahan organik diperairan memiliki peran penting dalam mempertahankan keseimbangan ekosistem tambak silvofishery terutama pada petakan dengan persentase tutupan mangrove yang tinggi (>50%). Perencanaan tambak silvofishery dengan menggunakan jenis mangrove yang berbeda diduga akan memberikan hasil yang berbeda, karena adanya perbedaan pada jumlah serasah yang jatuh serta kandungan bahan aktif yang bersifat sebagai antibakteri yang dimiliki jenis mangrove yang berbeda pula.Metode yang digunakan adalah eksperimental skala lapangan dengan parameter Kelimpahan bakteri Heterotrof di perairan dan Kualitas Air yang meliputi pH, suhu dan salinitas.Dari hasil penelitian didapatkan bahwa Kelimpahan bakteri heterotroph pada petakan silvofisheries dengan mangrove Avicennia marina lebih tinggi daripada petakan silvofisheries mangrove Rhizophora mucronata dan parameter kualitas air yang mempengaruhi kelimpahan bakteri heterotroph adalah pH, sedangkan suhu serta salinitas tidak memberikan pengaruh terhadap kelimpahan bakteri heterotroph pada petakan silvofisheries. 
PEMANFAATAN GETAH Excoecaria agallocha SEBAGAI BAHAN AKTIF PEMBASMI HAMA TRISIPAN (Cerithidea sp.) Setyastuti, Tri Ari; Puspitasari, Indah; Sukamto, Dwi; Asmarany, Anja
Chanos Chanos Vol 20, No 1 (2022): Chanos chanos
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/chanos.v20i1.10512

Abstract

Chemicals as pest and disease control in aquaculture production are suspected to contain potential toxicity to human health as well as harm to the environment, so this study aimed to use mangrove plants as pesticides made of organic material for pest control in shrimp ponds. One of the pests that are very disturbing is trisipan (Telescopium telescopium) from the Mollusca group. This study aimed to identify the active compounds contained in the latex of Excoecaria agallocha, to determine the cytotoxicity activity of the latex of Excoecaria agallocha and to determine the LC50 of the latex of E. agallocha against Trisipan. This research was conducted from September to December 2019 at the Mangrove Center Pulokerto Pasuruan and the Fish Pathology Laboratory of the Marine and Fisheries Polytechnic of Sidoarjo. The method used is experimental 6 treatments with 3 replications.The results that can be concluded from this study are that the active compound content of Excoecaria agallocha latex has not been determined because it is still in the testing process, the cytotoxicity activity of Excoecaria agallocha sap in brackish water media is higher than the cytotoxicity activity in freshwater media and LC50 of resin E. agallocha against Trisipan was shown in Trisipan with brackish water media and a dose of 400 ppm.
Algae content estimation utilizing optical density and image processing method Kamaluddin, Muhammad Wafiq; Gunawan, Agus Indra; Setiawardhana, Setiawardhana; Dewantara, Bima Sena Bayu; Insivitawati, Era; Asmarany, Anja; Pratama, Ariesa Editya
International Journal of Electrical and Computer Engineering (IJECE) Vol 14, No 6: December 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijece.v14i6.pp6248-6257

Abstract

One of the factors that influence shrimp cultivation is the presence of algae. Precise knowing algae content in the pond is essential for effective management. Most research in the field of algae species carried out by researchers were observing Chlorella Sp. more than the other algae species, with a particular emphasis on substance concentrations. This study proposed non-invasive techniques for quantifying algae abundance, utilizing optical density (OD) and image processing (IP) methods. Three different algae species are frequently found in Indonesia i.e., Chlorella Sp., Thalassiosira Sp., and Skeletonema Sp. are used as sample. Those samples are cultured and prepared in a certain volume with a certain quantity. For experimental and observation purposes, those samples are then diluted into water based on percentage value. The experimental results provided RGB values, which were then used to establish polynomial equations. To verify these equations, two approaches were employed: synthetic image analysis and evaluation using additional data. The mean average error (MAE) was found to be 3.467 for IP method and 3.513 for OD method. It shows that IP method give better result compared to OD method in this study. However, it is very possible that the two methods will complement each other.
EFEKTIVITAS DAUN KETAPANG (Terminalia catappa) DAN BAWANG PUTIH (Allium sativum) SEBAGAI IMUNOSTIMULAN HERBAL PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) Insivitawati, Era; Wahidi, Budi Rianto; Asmarany, Anja; Hakimah, Nisa; Jayanti, Shara; Setyatuti, Tri Ari; Suseno, Dewi Nurmalita; Sugianti, Budi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 21, No 2 (2025): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.21.2.138-144

Abstract

Budidaya ikan nila (Oreochromis niloticus) berpotensi besar, namun rentan terinfeksi bakteri dan memerlukan solusi alternatif pengganti antibiotik sintetis. Daun ketapang (Terminalia catappa) dan bawang putih (Allium sativum) bersifat antimikroba dan imunostimulan, namun efektivitas dan formulasi optimal masih memerlukan penelitian lebih lanjut. Penelitian ini bertujuan mengevaluasi efektivitas kombinasi daun ketapang dan bawang putih untuk mencari alternatif pengendalian bakteri yang lebih aman, mengingat penggunaan obat berlebihan dapat memicu resistensi. Daun ketapang dan bawang putih dipilih sebagai imunostimulan herbal yang potensial menekan bakteri Aeromonas tanpa dampak negatif bagi lingkungan. Metode yang digunakan adalah eksperimen Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan yaitu  Perlakuan Kontrol tanpa pemberian ekstrak daun ketapang dan bawang putih, Perlakuan A (komposisi 75 % daun ketapang dan 25% bawang putih), Perlakuan B (komposisi 50 % daun ketapang dan 50% bawang putih), dan Perlakuan C (komposisi 25 % daun ketapang dan 75% bawang putih). Pengujian mencakup uji fitokimia, zona hambat, DPPH (2,2-Diphenyl-1-picrylhydrazyl), analisis kualitas air dan pertumbuhan ikan dengan uji statistik. Hasil menunjukkan kombinasi 50% daun ketapang dan 50% bawang putih (Perlakuan B) merupakan yang terbaik dan berpotensi meningkatkan daya tahan tubuh ikan dengan kadar alkaloid tertinggi sebesar 37,91% (b/b) dengan persentase Relative Percent Difference (RPD) 1,5%, IC50 Uji DPPH 3,79 mg/ml, menghambat pertumbuhan bakteri yang ditunjukkan dengan hasil zona hambat terbesar, pertumbuhan berat terbaik dengan ABW mencapai 17,60 ± 6,84 gram/ekor dan ADG 1,53 g/minggu. Penelitian ini mengindikasikan bahwa kombinasi daun ketapang dan bawang putih dapat menjadi imunostimulan alami yang ramah lingkungan dalam budidaya ikan nila.
THE GROWTH PERFORMANCE OF WHITE SNAPPER (Lates calcarifer) FINGERLINGS IN ODE AQUACULTURE AND AGRICULTURE HATCHERY UNIT, BRUNEI-MUARA BRUNEI DARUSSALAM DISTRICT Ritonga, Lusiana BR; Harijono, Teguh; Asmarany, Anja; Widodo, Agus; Aonullah, Asep Akmal; Akbar, M.Baihaqi; Affisramyraj, Rizky
Aurelia Journal Vol 7, No 1 (2025): April
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/aj.v7i1.14754

Abstract

White snapper (Lates calcarifer) is a high value aquaculture commodity with increasing market demand. This study aimed to analyzed the growth performance of white snapper fry during the nursery phase at the ODE Aquaculture and Agriculture Hatchery Unit, Brunei-Muara District, Brunei Darussalam, from March to June 2023.The nursery utilized controlled fiberglass tanks equipped with aeration and a flow-through water system. Two types of tanks were used: rectangular tanks measuring 4 × 1.5 × 1 m (capacity: 6,000 L) and circular tanks with a diameter of 2 m (capacity: 3,140 L). A total of 100,000 fry were stocked, with 30,000–40,000 fry per tank. Observed parameters included survival rate (SR), absolute length growth rate, absolute weight growth rate, specific growth rate (SGR), and feed conversion ratio (FCR). Water quality parameters measured were temperature, salinity, and pH. Results indicated an absolute length growth rate of 1.2 cm/week, surpassing previous studies reporting only 0.4 cm/week. The absolute weight growth rate was 1.3 g/week, exceeding the 0.6–1 g/week range reported in other research. The FCR was 0.33, demonstrating excellent feed efficiency. However, the survival rate was 30.77%, below the standard for controlled nursery systems (>50%), likely due to high stocking density and cannibalism. Harvest size ranged from 8 to 9 cm. Water quality remained stable, with temperatures between 31–32.5°C, pH levels from 7.6–8.3, and salinity ranging from 30–32 ppt. Despite the low survival rate attributed to high stocking density, the nursery phase at this facility achieved commendable growth rates compared to previous studies. This success is supported by stable water quality and the provision of high-protein feed.
EFEKTIVITAS IMUNOSTIMULAN EKSTRAK Gracillaria sp. TERHADAP BAKTERI Aeromonas salmonicida SECARA IN VIVO Jayanti, Shara; Asmarany, Anja; Yudana, IGP Gede Rumayasa
Chanos Chanos Vol 23, No 2 (2025): CHANOS CHANOS
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/chanos.v23i2.15474

Abstract

This study was determining the effect of immunostimulant from Gracillaria sp. on Aeromonas salmonicida bacteria in vivo. The parameters were the survival rate, total leukocytes (Cyprinus carpio) and IC50 of Gracillaria sp. as an immunostimulant for koi that infected with Aeromonas salmonicida. Gracillaria sp. extract can improve the immune system, as seen from immune parameters such as leukoocytes and health. Gracillaria sp. extract with 70% ethanol has a flavonoid content of 1.9 mg QE/g. Flavonoids are antioxidant that can increase the immune system to against pathogen. The application of immunostimulant is given orally by mixing with the cellulase enzyme as much as 7.2 mg/g then added to koi feed at a dose based on IC50 Gracillaria sp. which is 18.6 mg/ml. he study was conducted using 6 treatments where the results showed that the highest total of leukocytes was in fish fed with immunostimulants at a dose of 2IC50 and infected with Aeromonas sp (P2) of 37.12 x 104 cells/mm3. While the lowest total leukocytes were in P5 fish, which was 16.08 x 104 cells/mm3. The lowest survival rate was in P5 (Fish fed commercial feed infected with Aeromonas salmonicida) 80% and in addition the survival value of the fish was 100%. In P5 fish, the fish fins became soft, the fish scales fell out, the surface of the fish skin produced a lot of mucus and there were wrinkles on the fish's tail fins.Penelitian ini dilaksanakan untuk mengetahui pengaruh pemberian imunostimulan dari Gracillaria sp. terhadap bakteri Aeromonas salmonicida secara in vivo. Parameter yang diuji adalah tingkat kelangsungan hidup, total leukosit ikan koi (Cyprinus carpio) serta dosis efektif dari ekstrak Gracillaria sp. sebagai immunostimulan untuk ikan koi yang terimfeksi bakteri Aeromonas salmonicida. Ekstrak Gracilaria sp. cukup efektif digunakan sebagai bahan imunostimulan untuk meningkatkan sistem imun ikan, terlihat dari parameter imun seperti lekosit dan kesehatan ikan. Ekstark Gracillaria sp.dengan pelarut etanol 70 % terbukti memiliki kandungan flavonoid sebesar 1,9 mg QE/g. Flavonoid merupakan senyawa bioaktif yang dapat meningkatkan sistem imun terhadap infeksi patogen. Aplikasi imunostimulan diberikan secara per oral dengan pemberian enzim selulase sebanyak 7,2 mg/g yang ditambahkan pada pakan ikan koi dengan dosis berdasarkan IC50 Gracillaria sp. yaitu 18,6 mg/ml. Penelitian dilakukan menggunakan 6 perlakuan dimana hasil menunjukkan bahwa jumlah total sel leukosit tertinggi adalah pada ikan yang diberikan pakan berimunostimulan dengan dosis 2IC50 dan diinfeksi dengan Aeromonas sp (P2) sebesar 37,12 x 104 sel/mm3. Sedangkan total leukosit terendah pada ikan P5 yaitu sebesar sebesar 16,08 x 104 sel/mm3. Tingkat kelangsungan hidup paling rendah adalah pada P5 (Ikan diberikan pakan komersil dengan diinfeksi Aeromonas salmonicida) 80% dan selain itu nilai kelangsungan hidup ikan adalah 100%. Pada ikan P5 mengalami kelembekan sirip ikan, kerontokan sisik ikan, permukaan kulit ikan mengeluarkan lendir yang banyak serta geripis pada sirip ekor ikan.
Antibacterial Effectiveness of Yellow Turmeric (Curcuma longa) and White Turmeric (Curcuma zedoaria) Extracts against Aeromonas salmonicida: Antibacterial Effectiveness of Yellow Turmeric (Curcuma longa) and White Turmeric (Curcuma zedoaria) Extracts against Aeromonas salmonicida Nisa Hakimah; Budi Rianto Wahidi; Tri Ari Setyastuti; Anja Asmarany; Era Insivitawati; Budi Sugianti; Dewi Nurmalita Suseno; Shara Jayanti
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.87885

Abstract

Aeromonas salmonicida is a major fish pathogenic bacterium responsible for significant losses in aquaculture. The growing concern over antibiotic resistance has driven interest in plant-based antibacterial alternatives. This study examined the extraction yield, phytochemical characteristics, and in vitro antibacterial activity of ethanolic extracts obtained from the rhizomes of Curcuma longa and Curcuma zedoaria against Aeromonas salmonicida. Extraction was conducted using an ethanol-based maceration technique. Qualitative phytochemical screening was subsequently performed, and antibacterial activity was evaluated through the disc diffusion assay at extract concentrations ranging from 10% to 100%. The results showed that C. longa produced a higher extraction yield (7.18%) than C. zedoaria (3.19%). Both extracts contained flavonoids, total phenolics, alkaloids, and tannins, while saponins were not detected. Antibacterial activity against A. salmonicida increased with extract concentration, reaching maximum inhibition at 100%, with zone diameters of 12.97 ± 0.57 mm (C. longa) and 12.17 ± 0.61 mm (C. zedoaria). Significant differences were observed among concentration treatments. In conclusion, ethanolic extracts of Curcuma longa and Curcuma zedoaria demonstrate promising plant-based antibacterial activity against A. salmonicida in vitro, supporting their potential use in aquaculture health management. Nevertheless, further in vivo studies and toxicity evaluations are required before practical application.
EVALUASI KINERJA PRODUKSI DAN EFISIENSI PAKAN PADA BUDIDAYA UDANG VANAMEI (Litopenaeus vannamei) SISTEM INTENSIF Asep Akmal Aonullah; Anja Asmarany; Lusiana BR Ritonga; Dian Lestari
Chanos Chanos Vol 24, No 1 (2026): CHANOS CHANOS
Publisher : Politeknik Kelautan dan Perikanan Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/chanos.v24i1.17638

Abstract

The main issue addressed in this study is the application of intensive whiteleg shrimp (Litopenaeus vannamei) culture at PT Padak Goar, East Lombok Regency, Indonesia. The objective of this study is to determine the technical parameters of intensive Vannamei shrimp farming, including stocking density, feed management, growth performance (ABW and ADG), feed efficiency (FCR), survival rate (SR), and their relationship with water quality.. The study was conducted in two grow-out ponds with surface areas of 7,648 m2 (B4) and 7,680 m2 (B5). Data collection covered stocking density, Average Body Weight (ABW), Feed Conversion Ratio (FCR), and Survival Rate (SR). The observation results were then analyzed using a quantitative descriptive approach to assess feed consumption in relation to production parameters, particularly FCR and shrimp growth. The results showed that in pond B4, the stocking density was 124 ind/m2, ABW was 14.3 g, ADG ranged from 0.24 to 0.37 g/day, FCR was 1.7, and SR was 70%, while in pond B5, the stocking density was 125 ind/m2, ABW was 12.9 g, ADG ranged from 0.21 to 0.33 g/day, FCR was 1.9, and SR was 66%. The results indicate that the intensive culture system is capable of supporting good shrimp growth and survival; however, differences in feed efficiency between ponds suggest that water quality and feed management play important roles in determining production performance. Permasalahan dalam penelitian ini adalah bagaimana penerapan sistem budidaya udang Vannamei (Litopenaeus vannamei) secara intensif di PT Padak Goar Kabupaten Lombok Timur. Tujuan penelitian ini adalah untuk mengetahui parameter teknis budidaya intensif udang Vannamei meliputi meliputi padat tebar, manajemen pakan, pertumbuhan (ABW dan ADG), efisiensi pakan (FCR), tingkat kelangsungan hidup (SR), serta keterkaitannya dengan kualitas air. Penelitian dilakukan dengan metode observasi lapangan pada dua petak tambak dengan luas masing-masing 7.648 m2 (B4) dan 7.680 m2 (B5). Data yang diamati mencakup padat tebar benur, Average Body Weight (ABW), Feed Conversion Ratio (FCR), serta Survival Rate (SR). Hasil pengamatan kemudian dianalisis secara deskriptif kuantitatif untuk konsumsi pakan terhadap parameter produksi, khususnya FCR dan pertumbuhan udang. Terjemhkan ke bahasa Inggris yang baik dan benar. Hasil penelitian menunjukkan bahwa pada petak B4 diperoleh padat tebar 124 ekor/m2, ABW 14,3 g, ADG 0,24–0,37 g/hari, FCR 1,7, dan SR 70%, sedangkan pada petak B5 diperoleh padat tebar 125 ekor/m2, ABW 12,9 g, ADG 0,21–0,33 g/hari, FCR 1,9, dan SR 66%. Hasil penelitian menunjukkan bahwa sistem budidaya intensif mampu mendukung pertumbuhan dan kelangsungan hidup udang yang baik; namun, perbedaan efisiensi pakan antar petak mengindikasikan bahwa kualitas air dan manajemen pakan berperan penting dalam menentukan kinerja produksi.