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All Journal PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Impresi Indonesia Heca Journal of Applied Sciences Malacca Pharmaceutics Grimsa Journal of Science Engineering and Technology
Kepel, Billy Johnson
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Journal : Grimsa Journal of Science Engineering and Technology

 1 
In Vitro Analysis of Anti-Inflammatory Effects of Purple Chrysanthemum (Chrysanthemum morifolium) Leaf Extract Nastiti, Gemi; Fatimawali, Fatimawali; Budiarso, Fona Dwiana Hermina; Manampiring, Aaltje Ellen; Kepel, Billy Johnson; Bodhi, Widdhi
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 1 (2025): April 2025
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i1.70

Abstract

Chronic inflammatory diseases are the most significant cause of death in the world and pose the greatest threat to human health. Treatment of inflammation is usually done by taking anti-inflammatory drugs that have dangerous side effects in long-term use. Chrysanthemum is the largest horticultural commodity in North Sulawesi that also holds great potential in the world of health. Based on its phytochemical content, purple chrysanthemum leaves have potential as an anti-inflammatory agent. This study aimed to analyze the anti-inflammatory activity of ethanol extract of purple chrysanthemum leaves using the red blood cell membrane stabilization method. This study used an in vitro laboratory experimental method using rat blood. The anti-inflammatory activity of the extract was tested using the red blood cell membrane stabilization method at 4 concentration series, namely 50 ppm, 100 ppm, 200 ppm, and 400 ppm, then measured on a UV-Vis spectrophotometer. The results of phytochemical screening showed that the ethanol extract of purple chrysanthemum (Chrysanthemum morifolium) leaves contains active compounds such as flavonoids, alkaloids, tannins, steroids, and phenolics. The ethanol extract of purple chrysanthemum leaves exhibits anti-inflammatory activity, with higher extract concentrations demonstrating stronger anti-inflammatory effects. The stability percentage values obtained at 50, 100, 200, and 400 ppm were 28.87%, 41.85%, 47.41%, and 64.15% with an IC50 of 235.88 ppm and a correlation coefficient of 0.94.
Efficacy of Purple Chrysanthemum Ethanol Extract in Inhibiting Staphylococcus aureus and Escherichia coli Growth Paukiran, Degol; Budiarso, Fona; Manampiring, Aaltje; Fatimawali, Fatimawali; Kepel, Billy Johnson; Bodhi , Widdhi
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 1 (2025): April 2025
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i1.63

Abstract

Staphylococcus aureus, a Gram-positive bacterium, and Escherichia coli, a Gram-negative bacterium, are known to cause both enteric and systemic infections in humans. The improper use of antibiotics has often been associated with the emergence of antibiotic resistance. Purple chrysanthemum (Chrysanthemum morifolium) has shown significant potential as an alternative treatment, owing to its strong antioxidant, anti-inflammatory, and antibacterial properties. These properties are attributed to the presence of bioactive compounds such as polyphenols, flavones, essential oils, and other phytochemicals. This study aimed to identify secondary metabolites and evaluate the antibacterial activity of ethanol extracts from purple chrysanthemum (Chrysanthemum morifolium). The extraction process was conducted using Ultrasonic-Assisted Extraction (UAE), and the antibacterial activity was assessed using the disc diffusion method at extract concentrations of 12.5%, 25%, and 50%, with ciprofloxacin serving as the positive control. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, phenolics, and steroids in the ethanol extract of purple chrysanthemum. Importantly, all ethanol extracts demonstrated antibacterial activity. The highest inhibition zones were observed at a 50% concentration, with S. aureus showing an inhibition zone of 12.35 ± 0.4 mm, while E. coli exhibited an inhibition zone of 15.08 ± 0.22 mm.
Investigating the Anti-Inflammatory Potential of Ethanol Extracts from Purple Chrysanthemum Flowers Sumakul, Gilbert Samuel; Fatimawali, Fatimawali; Bodhi, Widdhi; Budiarso, Fona Hermina Dwiana; Kepel, Billy Johnson; Manampiring, Aaltje
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 2 (2025): October 2025 (In Press)
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i2.69

Abstract

Indonesia has high biodiversity, with around 9,600 species of flora possessing medicinal properties. One such plant is the purple chrysanthemum (Chrysanthemum morifolium), which is rich in bioactive compounds with anti-inflammatory potential. The purple chrysanthemum is used in traditional medicine to treat various diseases and has demonstrated significant anti-inflammatory activity in vitro. Given the high prevalence of inflammatory diseases in Indonesia and the side effects associated with conventional drugs, this plant is considered a promising alternative therapy that may be safer and more effective. The aim of this study was to identify the phytochemical compounds and evaluate the anti-inflammatory activity of an ethanol extract from purple chrysanthemum flowers (Chrysanthemum morifolium) in vitro. The extract was prepared using 96% ethanol, and its anti-inflammatory activity was assessed using the red blood cell membrane stabilization method. Blood samples were collected from Wistar rats. The inhibitory concentration (IC50) values of the extract and Diclofenac Sodium were 203.08 ppm and 224.04 ppm, respectively. These findings indicate that the ethanol extract of purple chrysanthemums exhibits anti-inflammatory activity
Co-Authors Aaltje Manampiring B H. R. Kairupan Barasarathi , Jayanthi Bodhi , Widdhi Budiarso, Fona Hermina Dwiana Celik, Ismail Fatimawali . Fona Budiarso Gemi Nastiti Hermina Budiarso, Fona Dwiana Malino, Angeline Priscillia Manampiring, Aaltje Ellen Martha Marie Kaseke Niode, Nurdjannah Jane Paukiran, Degol Rumangu, Chrisa P. Saragih, Nathin Loria , Sumakul, Gilbert Samuel Tendean, Lydia Estelina Naomi Tendean, Widya TRINA EKAWATI TALLEI Widdhi Bodhi Wungouw, Herlina Ineke Surjane