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All Journal PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Impresi Indonesia Heca Journal of Applied Sciences Malacca Pharmaceutics Grimsa Journal of Science Engineering and Technology
Kepel, Billy Johnson
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In Vitro Test of Antioxidant Activity of Leilem Leaf Ethanol Extract (Clerodendrum minahassae) Using DPPH and FRAP Methods Malino, Angeline Priscillia; Kepel, Billy Johnson; Budiarso, Fona Dwiana Hermina; Fatimawali, Fatimawali; Manampiring, Aaltje Ellen; Bodhi, Widdhi
Heca Journal of Applied Sciences Vol. 2 No. 1 (2024): March 2024
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/hjas.v2i1.135

Abstract

The risk of degenerative diseases is increasing due to unhealthy lifestyles. Many people consume junk food, smoke, and do not protect themselves from sun exposure and pollution, exposing their bodies to free radicals. Free radicals can cause oxidative stress, necessitating the need for antioxidants to neutralize them. The leilem plant (Clerodendrum minahassae) is an endemic plant from North Sulawesi that is often used in traditional dishes and medicine. Moreover, leilem leaves are believed to be a source of natural antioxidants. This study aimed to determine the efficacy of leilem leaf ethanol extract as a source of antioxidants. The antioxidant potential was evaluated using phytochemical screening and antioxidant assays such as 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods, with ascorbic acid as a comparison. The level of antioxidant activity was measured by determining the IC50 value, which corresponds to the concentration of extract required to inhibit 50% of free radicals. The results of phytochemical screening showed that the ethanol extract of leilem (Clerodendrum minahassae) leaves contains active compounds such as phenols, flavonoids, saponins, tannins, steroids/triterpenoids, and alkaloids, indicating a high potential for antioxidant activity. The IC50 value for the DPPH method was 78.799 ppm, and the IC50 value for the FRAP method was 92.127 ppm. These results demonstrate that the ethanol extract of leilem leaves has strong antioxidant activity in both the DPPH and FRAP methods.
PENGARUH PENYULUHAN KESEHATAN TERHADAP TINGKAT PENGETAHUAN DAN SIKAP MENGENAI KESEHATAN REPRODUKSI PADA REMAJA SMA ADVENT TOMPASO II Tendean, Widya; Tendean, Lydia Estelina Naomi; Kepel, Billy Johnson; Kairupan, Bernabas Harold Ralph; Kaseke, Martha Marie
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 1 (2025): APRIL 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i1.41172

Abstract

Kesehatan Reproduksi merupakan suatu kondisi yang dinyatakan sehat secara sistem, fungsi dan proses dari reproduksi itu sendiri, yang merupakan hak setiap manusia. Desain penelitian yang digunakan dalam penelitian ini menggunakan rancangan quasi-experimental one group pretest-posttest design. Tujuan penelitian ini yaitu menganalisis pengaruh penyuluhan Kesehatan terhadap tingkat pengetahuan dan sikap mengenai Kesehatan reproduksi pada remaja SMA Advent Tompaso II. Sampel dalam penelitian ini adalah siswa-siswi SMA Advent Tompaso II yang duduk dibangku kelas X dan XI berjumlah 74 responden dengan menggunakan teknik total sampling.  Variabel dalam penelitian ini terdiri dari variabel bebas yaitu penyuluhan kepada remaja siswa SMA Advent Tompaso II, dan variabel terikat adalah tingkat pengetahuan remaja yang tinggal diasrama dan di luar asrama tentang Kesehatan reproduksi. Pengambilan data dilakukan dengan wawancara menggunakan kuesioner. Data penelitian ini dianalisis menggunakan analisis univariat dan bivariat. Hasil analisis menunjukkan bahwa terdapat pengaruh yang signifikan pada intervensi penyuluhan memperoleh nilai 0,001. Kesimpulan  terdapat pengaruh penyuluhan kesehatan terhadap tingkat pengetahuan dan sikap mengenai Kesehatan reproduksi pada remaja SMA Advent Tompaso II
In Vitro Analysis of Anti-Inflammatory Effects of Purple Chrysanthemum (Chrysanthemum morifolium) Leaf Extract Nastiti, Gemi; Fatimawali, Fatimawali; Budiarso, Fona Dwiana Hermina; Manampiring, Aaltje Ellen; Kepel, Billy Johnson; Bodhi, Widdhi
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 1 (2025): April 2025
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i1.70

Abstract

Chronic inflammatory diseases are the most significant cause of death in the world and pose the greatest threat to human health. Treatment of inflammation is usually done by taking anti-inflammatory drugs that have dangerous side effects in long-term use. Chrysanthemum is the largest horticultural commodity in North Sulawesi that also holds great potential in the world of health. Based on its phytochemical content, purple chrysanthemum leaves have potential as an anti-inflammatory agent. This study aimed to analyze the anti-inflammatory activity of ethanol extract of purple chrysanthemum leaves using the red blood cell membrane stabilization method. This study used an in vitro laboratory experimental method using rat blood. The anti-inflammatory activity of the extract was tested using the red blood cell membrane stabilization method at 4 concentration series, namely 50 ppm, 100 ppm, 200 ppm, and 400 ppm, then measured on a UV-Vis spectrophotometer. The results of phytochemical screening showed that the ethanol extract of purple chrysanthemum (Chrysanthemum morifolium) leaves contains active compounds such as flavonoids, alkaloids, tannins, steroids, and phenolics. The ethanol extract of purple chrysanthemum leaves exhibits anti-inflammatory activity, with higher extract concentrations demonstrating stronger anti-inflammatory effects. The stability percentage values obtained at 50, 100, 200, and 400 ppm were 28.87%, 41.85%, 47.41%, and 64.15% with an IC50 of 235.88 ppm and a correlation coefficient of 0.94.
Appraisal of Antioxidant Potential in Broccoli Microgreens under Different Drying Techniques Utilizing In Vitro and in Silico Methods Tallei, Trina Ekawati; Wungouw, Herlina Ineke Surjane; Kepel, Billy Johnson; Fatimawali, Fatimawali; Celik, Ismail; Niode, Nurdjannah Jane; Barasarathi , Jayanthi
Malacca Pharmaceutics Vol. 3 No. 1 (2025): March 2025
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v3i1.259

Abstract

Broccoli microgreens, rich in bioactive compounds, offer health benefits aligned with SDG 3: “Good Health and Well-Being.” Their antioxidants combat oxidative stress tied to chronic diseases, but drying can affect their activity. This study assessed the antioxidant capacities of fresh, microwave-dried, and air-fryer-dried broccoli microgreens using in vitro (DPPH assay) and in silico (molecular docking and dynamics) methods. The microgreens were cultivated under controlled conditions and dried using microwave and air-fryer techniques. Antioxidant activity was evaluated using the DPPH assay using ethanolic extracts. The bioactive compounds of fresh microgreens, detected through GC-MS, were analyzed in silico to evaluate their interactions with the target proteins CYP2C9 and NOX2. The findings revealed that air-fryer-dried microgreens demonstrated the highest DPPH activity, followed by fresh microgreens, while microwave-dried samples exhibited the lowest activity. GC-MS analysis of fresh samples revealed the presence of various compounds, including acids, ketones, sulfides, heterocycles, alcohols, esters, aromatic compounds, phthalate ester, and aldehydes. Molecular docking revealed strong interactions of certain compounds in fresh samples and CYP2C9 and NOX2, suggesting therapeutic potential against oxidative stress. Molecular dynamics simulations (MDS) showed stable binding for the CYP2C9-Methyl myristate complex, while the NOX-(Z)-1,2-Diphenylethene complex displayed weaker stability. In conclusion, broccoli microgreens show potential in mitigating oxidative stress, with air-fryer drying slightly enhancing their antioxidant activity. The antioxidant capacity of fresh microgreens is comparable to that of air-fryer-dried microgreens. In silico analyses demonstrate stable interactions between compounds in fresh microgreens and key proteins implicated in oxidative stress.
IN VITRO ANTIOXIDANT ACTIVITY TEST OF ETHANOL EXTRACT OF PINK CHRYSANTHEMUM LEAVES (CHRYSANTHEMUM MORIFOLIUM) USING DPPH AND FRAP METHODS Saragih, Nathin Loria ,; Kepel, Billy Johnson; Hermina Budiarso, Fona Dwiana; Fatimawali, Fatimawali; Manampiring, Aaltje Ellen
Jurnal Impresi Indonesia Vol. 4 No. 2 (2025): Indonesian Impression Journal (JII)
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jii.v4i2.6277

Abstract

Non-communicable diseases (NCDs) are a major global health concern, with degenerative diseases accounting for >80% of premature deaths. The combination of lifestyle and environmental factors increases the production of free radicals in the body, which, when uncontrolled, cause oxidative stress that significantly contributes to various diseases. Antioxidants play a key role in stopping free radical chain reactions. Although the body has endogenous antioxidants, additional exogenous antioxidants are also needed. One source of exogenous antioxidants is pink chrysanthemum leaves, commonly known as an ornamental plant, which shows potential as an antioxidant source. This study aims to analyze phytochemical content and antioxidant activity of pink chrysanthemum leaf ethanol extract using DPPH and FRAP methods, compared with ascorbic acid. This study used laboratory experimental methods. The qualitative color tests for phytochemical screening. Antioxidant activity tested via DPPH and FRAP methods using UV-Vis spectrophotometer at 517 and 739 nm wavelengths. Results : Phytochemical screening showed positive results for steroids, tannins, phenols, flavonoids, saponins, and alkaloids. DPPH method, analyzed using regression line equation Y = 0.3547x + 45.267, showed IC50 13.344, while the ascorbic acid's 3.030. FRAP method, analyzed using regression line equation Y = 0.773x – 27.6, showed IC50 100.388, while the ascorbic acid's 18.174. The ethanol extract of pink chrysanthemum leaves contains steroids, tannins, phenols, flavonoids, saponins, and alkaloids, and possesses antioxidant activity that falls into the very strong category using the DPPH method and the strong category using the FRAP method.
Efficacy of Purple Chrysanthemum Ethanol Extract in Inhibiting Staphylococcus aureus and Escherichia coli Growth Paukiran, Degol; Budiarso, Fona; Manampiring, Aaltje; Fatimawali, Fatimawali; Kepel, Billy Johnson; Bodhi , Widdhi
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 1 (2025): April 2025
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i1.63

Abstract

Staphylococcus aureus, a Gram-positive bacterium, and Escherichia coli, a Gram-negative bacterium, are known to cause both enteric and systemic infections in humans. The improper use of antibiotics has often been associated with the emergence of antibiotic resistance. Purple chrysanthemum (Chrysanthemum morifolium) has shown significant potential as an alternative treatment, owing to its strong antioxidant, anti-inflammatory, and antibacterial properties. These properties are attributed to the presence of bioactive compounds such as polyphenols, flavones, essential oils, and other phytochemicals. This study aimed to identify secondary metabolites and evaluate the antibacterial activity of ethanol extracts from purple chrysanthemum (Chrysanthemum morifolium). The extraction process was conducted using Ultrasonic-Assisted Extraction (UAE), and the antibacterial activity was assessed using the disc diffusion method at extract concentrations of 12.5%, 25%, and 50%, with ciprofloxacin serving as the positive control. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, phenolics, and steroids in the ethanol extract of purple chrysanthemum. Importantly, all ethanol extracts demonstrated antibacterial activity. The highest inhibition zones were observed at a 50% concentration, with S. aureus showing an inhibition zone of 12.35 ± 0.4 mm, while E. coli exhibited an inhibition zone of 15.08 ± 0.22 mm.
Investigating the Anti-Inflammatory Potential of Ethanol Extracts from Purple Chrysanthemum Flowers Sumakul, Gilbert Samuel; Fatimawali, Fatimawali; Bodhi, Widdhi; Budiarso, Fona Hermina Dwiana; Kepel, Billy Johnson; Manampiring, Aaltje
Grimsa Journal of Science Engineering and Technology Vol. 3 No. 2 (2025): October 2025 (In Press)
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v3i2.69

Abstract

Indonesia has high biodiversity, with around 9,600 species of flora possessing medicinal properties. One such plant is the purple chrysanthemum (Chrysanthemum morifolium), which is rich in bioactive compounds with anti-inflammatory potential. The purple chrysanthemum is used in traditional medicine to treat various diseases and has demonstrated significant anti-inflammatory activity in vitro. Given the high prevalence of inflammatory diseases in Indonesia and the side effects associated with conventional drugs, this plant is considered a promising alternative therapy that may be safer and more effective. The aim of this study was to identify the phytochemical compounds and evaluate the anti-inflammatory activity of an ethanol extract from purple chrysanthemum flowers (Chrysanthemum morifolium) in vitro. The extract was prepared using 96% ethanol, and its anti-inflammatory activity was assessed using the red blood cell membrane stabilization method. Blood samples were collected from Wistar rats. The inhibitory concentration (IC50) values of the extract and Diclofenac Sodium were 203.08 ppm and 224.04 ppm, respectively. These findings indicate that the ethanol extract of purple chrysanthemums exhibits anti-inflammatory activity
Evaluating the Efficacy of Clerodendrum minahassae Ethanol Extract on Insulin Regulation in Diabetic Wistar Rats Rumangu, Chrisa P.; Fatimawali, Fatimawali; Manampiring, Aaltje Ellen; Kepel, Billy Johnson; Budiarso, Fona Dwiana Hermina; Bodhi, Widdhi
Malacca Pharmaceutics Vol. 2 No. 1 (2024): March 2024
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v2i1.137

Abstract

Leilem plant (Clerodendrum minahassae Teisjm & Binn.) from the genus Clerodendrum has the potential as antidiabetic, antihypertensive, anti-inflammatory, antioxidant, antimalarial, antitumor, antidiarrheal, antimicrobial and antihyperlipidemic. This study aimed to see the effect of ethanol extract of Clerodendrum minahassae (CM) leaves on increasing insulin levels in diabetic Wistar rats induced with streptozotocin. This study was conducted in vivo, using 20 rats as experimental animals. The experimental animals were divided into four groups, namely the negative control group (Na-CMC 0.5%), the ethanol extract group of leilem leaves 250 mg and 500 mg, and the positive control group (glibenclamide) as a comparison. Each experimental animal was induced streptozotocin intraperitoneally; then, each solution was given for 14 days according to the test group. After the treatment, the animals were terminated for blood collection; the blood was then centrifuged to obtain blood plasma serum. Blood plasma serum was measured by the ELISA Kit (Rat/Mouse Insulin) method, and then the results were read on a spectrophotometric device. The results of the sample insulin concentration obtained showed that 250 mg/kgBW and 500 mg/kgBW of the CM ethanol extract group could increase insulin levels in diabetic Wistar rats, the same as the positive control group glibenclamide. In contrast, the Na-CMC 0.5% as a negative control group did not show a significant increase in insulin levels. Leilem leaves can be developed for further research on their antidiabetic activity both in vitro, in vivo, and in silico, as well as their toxicity.
Co-Authors Aaltje Manampiring B H. R. Kairupan Barasarathi , Jayanthi Bodhi , Widdhi Budiarso, Fona Hermina Dwiana Celik, Ismail Fatimawali . Fona Budiarso Gemi Nastiti Hermina Budiarso, Fona Dwiana Malino, Angeline Priscillia Manampiring, Aaltje Ellen Martha Marie Kaseke Niode, Nurdjannah Jane Paukiran, Degol Rumangu, Chrisa P. Saragih, Nathin Loria , Sumakul, Gilbert Samuel Tendean, Lydia Estelina Naomi Tendean, Widya TRINA EKAWATI TALLEI Widdhi Bodhi Wungouw, Herlina Ineke Surjane