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Response Surface Methodology to Optimize Laccase Produced from Pleurotus ostreatus Using Corn Cob and Rice Bran as Substrate Hermansyah, Hermansyah; Gozali, Maftuh; Julinar, Julinar; Fanani, Zainal; Loekitowati Hariani, Poedji
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.14472

Abstract

ABSTRACT. Laccase is an industrially significant enzyme capable of oxidizing a broad range of phenolic compounds, making it valuable for applications such as bioremediation, textile dye decolorization, and pulp bleaching. Therefore, this study aimed to optimize laccase production from Pleurotus ostreatus using agricultural waste substrates corn cob (BJ), rice bran (DP), and their 1:1 mixture (BJ:DP) through Response Surface Methodology (RSM). Extracellular laccase was obtained by centrifuging the cultured medium, followed by ammonium sulfate precipitation and dialysis. The enzyme activity was quantified, and the fraction with the highest specific activity was analyzed using SDS-PAGE. Furthermore, Central Composite Design (CCD) was used to assess the effects of three independent variables, namely ABTS concentration (0.01–0.1 mM), incubation temperature (20–30 °C), and reaction time (20–30 minutes). The results showed that for BJ substrate, the highest enzyme activity (13.7 ± 0.05 U/mL) was observed in the 0–20% ammonium sulfate fraction, with specific activities of 76.25 ± 0.09 U/mg and 90.28 ± 0.03 U/mg in the 0–20% and 20–40% fractions, respectively. Conversely, DP substrate achieved a maximum specific activity of 209.67 ± 0.028 U/mg in the 20–40% fraction. The crude extract from BJ:DP mixture showed a high protein content (0.636 ± 0.006 mg/mL) but the specific activity was substantially lower (19.33 ± 0.003 U/mg). Based on RSM analysis, the optimal conditions were ABTS concentration of 0.05 mM, incubation temperature of 22.21 °C, and reaction time of 28.64 minutes, resulting in a predicted laccase activity of 13.99 U/mL. Keywords: Corn cob, Laccase, Pleurotus ostreatus, Response Surface Methodology, Rice bran
Pelatihan pembuatan sabun transparan antibakteri dari ekstrak daun salam (Syzygium polyanthum) di desa Tanjung Baru Harmida, Harmida; Tanzerina, Nina; Lamin, Safrina; Salni, Salni; Aminasih, Nita; Loekitowati Hariani, Poedji
Abdimas Mandalika Vol 3, No 2 (2024): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/am.v3i2.21246

Abstract

Abstract:  The use of chemicals in soap can have a negative effect on sensitive skin and has the potential to pollute the environment. This community service activity aims to provide education about the importance of maintaining health, especially skin, and giving training in making transparent antibacterial soap with the addition of salam leaves extract (Syzygium polyanthum) in Tanjung Baru village, North Indralaya sub-district, Ogan Ilir. The activity participants were 30 young women and mothers. The activity method is carried out in stages: (i) education about infectious skin diseases and the importance of maintaining healthy skin, (ii) training on how to extract antibacterial compounds from salam leaves by maceration, (iii) training on making transparent soap, and (iv) activity evaluation. The activity results showed that 86.67% of participants did not understand antibacterial transparent soap before the training. After the activity, the percentage of understanding and very understanding increased to 98.67%. The soap products produced are very popular with the public (86.68%) based on aroma, shape, color, comfort, and foam characteristics. Through this activity, it is hoped that public awareness of skin health will increase, and the use of antibacterial transparent soap, proven safe, economical, and easy to make from natural ingredients, will also increase.Abstrak: Penggunaan bahan kimia dalam sabun dapat memberikan efek negatif pada kulit yang sensitif, dan berpotensi mencemari lingkungan. Kegiatan pengabdian masyarakat yang dilakukan ini bertujuan memberikan penyuluhan tentang pentingnya menjaga kesehatan terutama kulit, dan memberikan pelatihan pembuatan sabun transparan yang bersifat antibakteri dengan penambahan ekstrak daun salam (Syzygium polyanthum) di desa Tanjung Baru, kecamatan Indralaya Utara, Ogan Ilir. Peserta kegiatan adalah remaja putri dan ibu-ibu sebanyak 30 orang. Metode kegiatan dilaksanakan dengan tahapan (i) penyuluhan tentang infeksi penyakit kulit dan pentingnya menjaga kesehatan kulit, (ii) pelatihan cara mengekstraksi senyawa antibakteri dari daun salam dengan cara maserasi, (iii) pelatihan pembuatan sabun transparan, dan (iv) evaluasi kegiatan. Hasil kegiatan menunjukkan bahwa sebelum pelatihan sebanyak 86,67 % peserta kurang memahami tentang sabun transparan yang bersifat anti bakteri. Setelah kegiatan persentase paham dan sangat paham meningkat menjadi 98,67 %. Produk sabun yang dihasilkan sangat disukai masyarakat (86,68 %) berdasarkan karakteristik aroma, bentuk, warna, kenyamanan dan busa. Melalui kegiatan ini, diharapkan kesadaran masyarakat terhadap kesehatan kulit meningkat, serta penggunaan sabun transparan antibakteri yang terbukti aman, ekonomis, dan mudah dibuat dari bahan alami semakin meningkat pula.