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All Journal journal of Basic Medical Veterinary Jurnal Agro Veteriner
Larasati, Anissa Dhea
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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The Effect of Giving Pork Oil and Egg Yolk as Components of a High Fat Diet on The Hematological and Cholesterol Profiles of Mice (Mus musculus) Larasati, Anissa Dhea; Allailah, Rashya Jasmine; Syachfitri, Dea Dwyna; Safania, Mocca Cintaura Radya; Rachmania, Revira Yunita; Wardhana, Dewa Drio Putra; Gustiko, Aliyya Lintang; Faruq, Abdullah Umar Al
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.53179

Abstract

Background: This study examined the role of a high-fat diet, especially one containing animal fat, in its influence on the hematology of the organism, with mice as research subjects. Hematology, as the study of blood components, is the focus for analyzing the impact of a high-fat diet, which includes animal fats such as lard and egg yolks. Although fats are essential for cell function and nutrient absorption, excessive intake can increase the risk of serious health problems. Purpose: To fill the gap in knowledge by evaluating the effect of lard and egg yolk on the hematological profile of mice. Methods: True Experimental with a Pretest-Posttest Control Group design. The sampling technique used is Simple Random Sampling. The parameters yolk). The sampling technique used is Simple Random Sampling. The parameters observed include blood hematological profiles (Erythrocyte Count, Hemoglobin, and Erythrocyte Index) and cholesterol levels. Results: Expected to provide in-depth insight into the impact of high-fat diets on blood health, provide important contributions to understanding the human health implications, and support the development of more effective nutritional guidelines for managing diet and preventing metabolism-related diseases. Conclusion: It can be concluded that giving pork oil with added egg yolk can increase erythrocyte levels, hemoglobin profile, erythrocyte index (MCV and MCH), and cholesterol levels.
Detection of Salmonella sp. in broiler chickens in closed houses using the polymerase chain reaction method Larasati, Anissa Dhea; Sabdoningrum, Emy Koestanti; Nidom, Chairul Anwar; Hidanah, Sri; Rachmawati, Kadek; Puspitasari, Yulianna
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78646

Abstract

This study aimed to detect the presence of Salmonella sp. in broiler chickens raised in closed house systems using the Polymerase Chain Reaction (PCR) method. Liver and intestinal samples were collected from chickens showing clinical signs such as diarrhea, anorexia, and lethargy, along with pathological lesions observed during necropsy. Bacteriological identification involved isolation using selective media, Gram staining and biochemical tests. Two samples (3A and 3B) showed colony morphology and biochemical characteristics consistent with Salmonella sp. Confirmation using PCR targeting the invA gene (primers 139 and 141, expected amplicon size 284 bp) yielded negative results in both samples, while the positive control successfully amplified the target. The inconsistency between bacteriological and molecular results may be attributed to several factors: absence or mutation of the invA gene in the tested isolates, primer mismatch with local Salmonella strains, or inadequate DNA quality and concentration. These findings suggest that reliance on a single molecular marker may be insufficient for accurate detection and underscore the importance of optimizing PCR conditions. The study highlights the need for locally adapted primers and complementary diagnostic approaches to improve the reliability of Salmonella sp. detection in poultry, particularly in intensive production systems like closed house environments.
Co-Authors Allailah, Rashya Jasmine Faruq, Abdullah Umar Al Gustiko, Aliyya Lintang Hidanah, Sri Kadek Rachmawati Nidom, Chairul Anwar Rachmania, Revira Yunita Sabdoningrum, Emy Koestanti Safania, Mocca Cintaura Radya Syachfitri, Dea Dwyna Wardhana, Dewa Drio Putra Yulianna Puspitasari