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ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT Fachrial, Edy; Harmileni, Harmileni
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 5, No 1 (2018): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.

AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT YANG DIISOLASI DARI MAKANAN TRADISIONAL FERMENTASI KHAS BATAK “NANIURA” Aloysius, Aloysius; Ulfa, Anjurniza; Situmorang, Anggita Kasih Fianti; Harmileni, Harmileni; Fachrial, Edy
BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan Vol 6, No 1 (2019): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i1.2165

Lactic acid bacteria (LAB) could be isolated from various fermented food products. One potential source of LAB is traditional fermented food. The aim of the study was to isolate and investigate antimicrobial activity of LAB isolated from traditional Batak food, “naniura”. The LAB isolates were characterized by Gram staining, fermentation type and catalase test. The investigation of antimicrobial activity of LAB against pathogenic bacteria were conducted using disc diffusion method. The results showed that 6 isolates of BAL were successfully isolated namely BN1, BN2, BN3, BN4, BN5 and BN6 had characteristics of Gram positive, rod shaped and catalase negative. All selected isolate have heterofermentation type. Four isolates (BN1, BN2, BN5 and BN6) were able to inhibit S. aureus, E. coli and S. typhi with inhibition zone diameters ranging from 6,9 to 12,3 cm. Based on the result, it was concluded that LAB isolated from naniura has potential as a source of probiotics.

KEANEKARAGAMAN JAMUR PADA POHON KELAPA SAWIT DI PESISIR PANTAI PT. MOPOLI RAYA UNIT DAMAR CONDONG KAB. LANGKAT SUMATERA UTARA Rama Riana Sitinjak; Harmileni; Supriyadi
JURNAL PELITA KOTA Vol 2 No 2 (2021): JURNAL PELITA KOTA, AUGUST 2021
Publisher : Karimun University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51742/pelita.v2i2.420

Pentingnya dalam keanekaragaman tersebut termasuk berbagai jenis jamur yang tumbuh di Indonesia dan telah lama dikenal serta dimanfaatkan oleh masyarakat sumber pangan dan sebagai bahan obat. Jamur atau cendawan ini turut memberikan andil besar dalam memenuhi aneka ragam atau menu makanan khas Indonesia, seperti tape, tempe, oncom, tauco, roti, minuman fermentasi, serta berbagai macam makanan lainnya. Jamur juga berperan penting sebagai decomposer yang dapat membentuk jaring-jaring makanan bagi tumbuhan atau ekosistem hutan. Penelitian ini bertujuan untuk mengetahui tingkat keanekaragaman jamur pada pohon kelapa sawit dengan jenis-jenis. jamur di perkebunan pesisir pantai. Metode yang digunakan adalah metode purposive sampling, yaitu teknik pengambilan sampel dengan menentukan kriteria-kriteria tertentu. Parameter penelitian terdiri frekuensi mutlak (FM), frekuensi relatif (FR), kerapatan mutlak (KM), kerapatan relatif (KR), dominasi, indeks nilai penting, perbandingan nilai penting (H). Data dianalisis dengan indeks keanekaragaman Shannon-Wiener. Hasil penelitian menunjukkan bahwa tingkat keanekaragaman jamur pada pohon kelapa sawit di perkebunan pesisir pantai PT. Mopoli Raya Unit Damar Condong Kab. Langkat Sumatera Utara masih tergolong rendah dengan Nilai Indeks H’ 0.33.

ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT Edy Fachrial; Harmileni Harmileni
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 5, No 1 (2018): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v5i1.1707

The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.

AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT YANG DIISOLASI DARI MAKANAN TRADISIONAL FERMENTASI KHAS BATAK “NANIURA” Aloysius Aloysius; Anjurniza Ulfa; Anggita Kasih Fianti Situmorang; Harmileni Harmileni; Edy Fachrial
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 6, No 1 (2019): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i1.2165

Lactic acid bacteria (LAB) could be isolated from various fermented food products. One potential source of LAB is traditional fermented food. The aim of the study was to isolate and investigate antimicrobial activity of LAB isolated from traditional Batak food, “naniura”. The LAB isolates were characterized by Gram staining, fermentation type and catalase test. The investigation of antimicrobial activity of LAB against pathogenic bacteria were conducted using disc diffusion method. The results showed that 6 isolates of BAL were successfully isolated namely BN1, BN2, BN3, BN4, BN5 and BN6 had characteristics of Gram positive, rod shaped and catalase negative. All selected isolate have heterofermentation type. Four isolates (BN1, BN2, BN5 and BN6) were able to inhibit S. aureus, E. coli and S. typhi with inhibition zone diameters ranging from 6,9 to 12,3 cm. Based on the result, it was concluded that LAB isolated from naniura has potential as a source of probiotics.

PEMBUATAN BIOPESTISIDA DARI EKSTRAK DAUN SIRSAK (Annona muricata) UNTUK PENGENDALIAN HAMA ULAT API (Setothosea asigna V.Eecke) PADA TANAMAN KELAPA SAWIT (Elaeis guineensis Jacq) Gimelliya Saragih; Benny Rio Fernandez; Yunianto Yunianto; Harmileni Harmileni
JBIO: jurnal biosains (the journal of biosciences) Vol 5, No 1 (2019): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v5i1.12331

Ulat api (Setothosea asigna V.Eecke) merupakan hama pemakan daun yang terpenting di perkebunan kelapa sawit terutama di Sumatera Utara. Ulat ini dikenal sebagai ulat yang rakus yang sering menimbulkan kerugian di perkebunan kelapa sawit pada tanaman muda maupun tanaman tua. Pada perkebunan kelapa sawit, ulat pemakan daun kelapa sawit umumnya diatasi dengan menggunakan insektisida kimia sintetik yang mampu menurunkan populasi hama dengan cepat, sehingga dapat dihindari terjadinya kerusakan daun lebih lanjut. Walaupun demikian, penggunaan insektisida kimia sintetik secara kurang bijaksana telah terbukti dapat menimbulkan berbagai dampak negatif terhadap lingkungan. Tujuan penelitian ini adalah pembuatan biopestisida dari daun sirsak untuk mengendalikan hama ulat api (Setothosea asigna V.Eecke). Daun sirsak diekstrak dengan pelarut etanol 96%. Ekstrak pekat yang diperoleh diencerkan dengan akuades menjadi beberapa variasi konsentrasi yaitu 10%, 20%, 30%, 40% dan 50%. Hasil uji insektisida menunjukkan ekstrak daun sirsak 30% sudah efektif dalam mengendalikan hama ulat api. Dari hasil tersebut disimpulkan bahwa daun sirsak dapat dimanfaatkan sebagai biopestisida dalam mengendalikan hama ulat api dengan mekanisme racun kontak.

ISOLATION AND SCREENING OF AMYLASE PRODUCING BACTERIA FROM PALM OIL LIQUID WASTE Harmileni Harmileni; Rika Silvany; Tengku Rachmi Hidayani; Edy Fachrial
JBIO: jurnal biosains (the journal of biosciences) Vol 8, No 3 (2022): JBIO: jurnal biosains (the journal of biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v8i3.39886

Amylase is the most studied enzyme in biotechnology and also accounts for 25% of the total enzyme market worldwide. In industry, amylase has potential applications in the bakery, textile, detergent, syrup, pharmaceutical, and medical industries. One of the potential sources of amylase is microbes, because it is easy to isolate, culture, and also genetically engineered. Liquid waste is one of the most potential and abundant sources of microbes. In this study, the authors used palm oil waste as a source of amylase-producing microbes. The purpose of this study was to isolate and screen bacteria that showed amylase activity. Bacteria were isolated from palm oil liquid waste samples using the serial dilution method and the number of CFUs was calculated. The isolates were then characterized morphologically including Gram staining, elevation, color, edge, cell shape and also catalase reaction. Characterization results obtained 7 gram positive isolates and 8 gram negative isolates, the shape of cocci (12 isolates) and bacilli (3 isolates) and convex elevation and white isolates. Amylase activity was detected using nutrient agar media supplemented with 1% starch and the amylolytic index was measured. Amylolytic activity was indicated by the presence of a clear zone around the isolate. Of the 15 isolates, only 1 isolate showed amylase activity, namely LCF 4 with an amylolytic index of 0.57. Further research is recommended to identify and determine enzyme activity.Keywords : Activity; amylase; bacteria; palm oil liquid waste

Unveiling ZrO2/natural zeolite catalytic performance on hydrocracking palm oil mill effluent residue Junifa Layla Sihombing; Herlinawati Herlinawati; Ahmad Nasir Pulungan; Agus Kembaren; Gimelliya Saragih; Harmileni Harmileni; Rahayu Rahayu; Ary Anggara Wibowo
Jurnal Pendidikan Kimia (JPKIM) Vol 15, No 2 (2023): August
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v15i2.43324

Palm oil mill effluent (POME) is the largest liquid waste from crude palm oil production. This liquid waste still contains a lot of chemical components, solid deposits, and oil which is dangerous if released directly into the environment. The residual oil and grease components contained in POME can be further extracted and converted into fuel fractions. This study investigates the conversion of residual oil from POME into fuel fractions through hydrocracking. A ZrO2/Sarulla natural zeolite (SNZ) catalyst was used, characterized by a particle size of 1-1.5 µm, a surface area of 73.3 m2/g, a pore volume of 0.161 cc/g, and a pore diameter of 3.35 nm. The effect of catalyst mass was studied, with the total conversion increasing to a certain extent with increasing catalyst mass, however, an increase in coke formation decreased the product yield. The highest gasoline fraction selectivity was obtained with a catalyst mass of 0.09 wt% (~42%), while the kerosene fraction was most obtained with a catalyst mass of 0.15 wt% (~40%). The liquid product with a catalyst mass of 0.15 wt% had the highest HHV of 44.2 MJ/kg, a 12% increase from the HHV of POME oil residue (39.4 MJ/kg). The results demonstrate the potential of using residual oil from POME as a source for fuel production and the use of natural zeolite-based catalysts as hydrocracking catalysts.Keywords: Biofuel; Hydrocracking; Natural zeolite; Oil extracted; Palm oil mill effluent

Aktivitas antibakteri bakteri endofit daun kelapa sawit (Elaeis guineensis) terhadap Escherichia coli dan Staphylococcus aureus Harmileni, Harmileni; Saragih, Gimelliya; Hidayani, Tengku Rachmi; Mirnandaulia, Meutia
Jurnal Prima Medika Sains Vol. 5 No. 1 (2023): Juni
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v5i1.3755

Mikroorganisme endofit adalah mikroorganisme yang terdapat dalam jaringan tanaman dan memiliki kemiripan sifat senyawa bioaktif dengan tanaman inangnya. Daun kelapa sawit diketahui memiliki aktivitas antibakteri, tetapi penelitian mengenai aktivitas antibakteri bakteri endofitnya belum pernah dilaporkan. Tujuan penelitian ini adalah mengisolasi bakteri endofit dari daun kelapa sawit serta menentukan aktivitas antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Delapan isolat bakteri endofit berhasil diisolasi dari daun kelapa sawit. Satu isolat termasuk bakteri Gram negatif dan tujuh isolat adalah bakteri Gram positif. Analisa morfologi menunjukkan satu isolat berbentuk basil dan yang lain berbentuk kokus. Uji antibakteri menunjukkan satu isolat (IDS18) menunjukkan aktivitas antibakteri yang kuat terhadap S.aureus dengan zona hambat 13 mm, tetapi tidak menunjukkan aktivitas antibakteri terhadap E. coli. Sementara lima isolat yaitu IDS1, IDS10, IDS11, IDS14 dan IDS16 menunjukkan aktivitas yang lemah terhadap E.coli, tetapi tidak memiliki aktivitas terhadap S.aureus. Hasil penelitian menunjukkan bahwa bakteri endofit daun kelapa sawit berpotensi sebagai penghasil senyawa antibakteri. Diperlukan penelitian lebih lanjut mengenai optimasi produksi senyawa bioaktif antibakteri serta karakterisasinya.

Antibacterial activity of palm leaf extract against Propionibacterium acnes and Staphylococcus aureus Harmileni, Harmileni; Hidayani, Tengku Rachmi; Chiuman, Linda; Marfitania, Taranipa
Jurnal Prima Medika Sains Vol. 6 No. 1 (2024): June
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v6i1.5258

Acne vulgaris is the most common skin disorder in adults and young adults. The growth of acne is caused by many factors, including excess sebum secretion and changes in microbial flora, mainly the colonization of Propionibacterium acnes and Staphylococcus aureus. This study aimed to determine the anti-bacterial activity of palm leaf extract against Propionibacterium acnes and Staphylococcus aureus. The research procedure includes palm leaf extraction and anti-bacterial tests. Palm leaf extraction was carried out using ultrasonication, while the anti-bacterial test was carried out using disc diffusion. The palm leaf extract obtained was diluted to 10, 20, 30, 50, and 50%, and each variation of this concentration was tested for anti-bacterial activity. The results of the anti-bacterial test showed that all palm leaf extract samples from 10% to 50% concentration could inhibit the growth of P. acnes and S. aureus bacteria. The 50% extract of ultrasonicated palm leaves had the largest inhibition zone diameter of 13.8 mm for P. acnes bacteria and 10.1 for S. aureus bacteria. From the results of this study, palm leaf extract has the potential to be used as a product for the prevention or treatment of acne vulgaris.

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