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All Journal Medic Nutricia : Jurnal Ilmu Kesehatan Vitalitas Medis : Jurnal Kesehatan Dan Kedokteran
Yayuk Putri Rahayu
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Biochemistry, Genetics & Molecular Biology Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI SEDIAAN SABUN PADAT EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Staphylococcus aureus Titin Agustina; Yayuk Putri Rahayu; Minda Sari Lubis; D.Elysa Putri Mambang
Medic Nutricia : Jurnal Ilmu Kesehatan Vol. 2 No. 4 (2024): Medic Nutricia : Jurnal Ilmu Kesehatan
Publisher : Cahaya Ilmu Bangsa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5455/nutricia.v2i4.2645

Abstract

Tanaman mengkudu (Morinda citrifolia L.) digunakan untuk pengobatan herbal, yang memiliki senyawa antraquionon, senyawa asam amino triphophan, arginin, asam ursolat, flavonoid yang dapat menurunkan lemak dalam darah. Tujuan penelitian ini untuk mengetahui ekstrak etanol daun mengkudu dapat dijadikan formulasi sediaan sabun padat dan untuk mengetahui apakah sediaan sabun padat ekstrak etanol daun mengkudu memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus. Metode penelitian dilakukan secara eksperimental dengan variable bebas dan variabel terikat. Hasil penelitian menunjukkan bahwa serbuk simplisia dan ekstrak etanol daun mengkudu mengandung senyawa kimia alkaloid, flavonoid, saponin, tanin, steroid/triterpenoid, dan glikosida. Hasil uji mencakup, hasil uji organoleptik, hasil uji homogenitas, hasil uji iritasi, hasil uji tinggi busa dan stabilitas busa, hasil uji kelembaban, hasil uji kesukaan relawan. Memiliki hasil nilai suka pada sediaan sabun padat daun mengkudu dengan aktivitas antibakteri pada konsentrasi 10%, 20%, 30%, daya hambat 23,36 mm, 24,76 mm, dan 26,63 mm terhadap bakteri Staphylococcus aureus.
UJI SITOTOKSISITAS EKSTRAK DAUN ECENG GONDOK (Eichhornia crassipes) Mart.) Solms) PADA LARVA Artemia salina Leach DENGAN METODE BSLT Retno Sekarini; Daeng Elysa Putri Mambang; Anny Sartika Daulay; Yayuk Putri Rahayu
Medic Nutricia : Jurnal Ilmu Kesehatan Vol. 6 No. 2 (2024): Medic Nutricia : Jurnal Ilmu Kesehatan
Publisher : Cahaya Ilmu Bangsa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5455/nutricia.v6i2.5189

Abstract

Salah satu tanaman yang dapat dijadikan sebagai obat tradisional ialah Eceng Gondok (Eichhornia crassipes) Mart.) Solms) yang memiliki khasiat sebagai obat sakit gigi, obat gatal, dan penurun berat badan dengan kandungan senyawa Tanin, Flavonoid, Saponin, Glikosida, Steroid/triterpenoid, dan juga Alkaloid. Penelitian ini bertujuan untuk mengetahui metabolit sekunder yang terkandung dalam simplisia daun eceng gondok dan sitotoksisitas dari ekstrak etanol daun eceng gondok ekstrak etanol daun eceng gondok dengan menentukan nilai LC50. Pada penelitian ini dilakukan karakterisasi simplisia, skrining fitokimia dan uji sitotoksisitas ekstrak daun eceng gondok. Uji sitotoksisitas ekstrak daun eceng gondok menggunakan metode (Brine shrimp lethality test) atau BSLT dengan konsentrasi 115µg/ml, 395µg/ml, 575µg/ml, 805µg/ml, dan 1150µg/ml dengan cara didiamkan selama 24 jam untuk melihat sitotoksisitas nya. Data yang diperoleh di analisis menggunakan analisa probit menggunakan excel. Hasil data yang diperoleh dengan metode Brine Shrimp Lehality Test menunjukkan nilai LC50 sebesar 335,5830µg/ml, sehingga dapat disimpulkan bahwa ekstrak etanol daun eceng gondokk bersifat sitotoksisitas dan memiliki sifat antikanker. Senyawa uji bersifat toksik jika LC50 kurang dari 1000 µg/ml.
UJI SITOTOKSISITAS EKSTRAK DAUN ECENG GONDOK (Eichhornia crassipes) Mart.) Solms) PADA LARVA Artemia salina Leach DENGAN METODE BSLT CYTOTOXICITY TEST OF HYACINTH LEAVES (Eichhornia crassipes) Mart.) Solms) IN LARVAE OF Artemia salina Leach BY THE BRINE Sekarini, Retno; Daeng Elysa Putri Mambang; Anny Sartika Daulay; Yayuk Putri Rahayu
Medic Nutricia : Jurnal Ilmu Kesehatan Vol. 6 No. 4 (2024): Medic Nutricia : Jurnal Ilmu Kesehatan
Publisher : Cahaya Ilmu Bangsa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5455/nutricia.v6i4.5225

Abstract

ABSTRAK Salah satu tanaman yang dapat dijadikan sebagai obat tradisional ialah Eceng Gondok (Eichhornia crassipes) Mart.) Solms) yang memiliki khasiat sebagai obat sakit gigi, obat gatal, dan penurun berat badan dengan kandungan senyawa Tanin, Flavonoid, Saponin, Glikosida, Steroid/triterpenoid, dan juga Alkaloid. Penelitian ini bertujuan untuk mengetahui metabolit sekunder yang terkandung dalam simplisia daun eceng gondok dan sitotoksisitas dari ekstrak etanol daun eceng gondok ekstrak etanol daun eceng gondok dengan menentukan nilai LC50. Pada penelitian ini dilakukan karakterisasi simplisia, skrining fitokimia dan uji sitotoksisitas ekstrak daun eceng gondok. Uji sitotoksisitas ekstrak daun eceng gondok menggunakan metode (Brine shrimp lethality test) atau BSLT dengan konsentrasi 115µg/ml, 395µg/ml, 575µg/ml, 805µg/ml, dan 1150µg/ml dengan cara didiamkan selama 24 jam untuk melihat sitotoksisitas nya. Data yang diperoleh di analisis menggunakan analisa probit menggunakan excel. Hasil data yang diperoleh dengan metode Brine Shrimp Lehality Test menunjukkan nilai LC50 sebesar 335,5830µg/ml, sehingga dapat disimpulkan bahwa ekstrak etanol daun eceng gondokk bersifat sitotoksisitas dan memiliki sifat antikanker. Senyawa uji bersifat toksik jika LC50 kurang dari 1000 µg/ml. Kata Kunci : Daun Eceng Gondok, Skrining Fitokimia, Uji Sitotoksitas, LC50 ABSTRACT One of the plants that can be used as traditional medicine is water hyacinth (Eichhornia crassipes) Mart.) Solms) which has properties as a toothache medicine, itching medicine, and weight loss with the content of tannins, flavonoids, saponins, glycosides, steroids/triterpenoids, and also alkaloids. This study aims to determine the secondary metabolites contained in water hyacinth leaf simplicia and the cytotoxicity of the ethanol extract of water hyacinth leaves by determining the LC50 value. In this research simplicia characterization, phytochemical screening and cytotoxicity test of water hyacinth leaf extract were carried out. Cytotoxicity test of water hyacinth leaf extract used the method (Brine shrimp lethality test) or BSLT with concentrations of 115µg/ml, 395µg/ml, 575µg/ml, 805µg/ml, dan 1150µg/ml by allowing it to stand for 24 hours to see its cytotoxicity. The data obtained were analyzed using probit analysis using excel. The results of the data obtained using the Brine Shrimp Lehality Test showed an LC50 value of 335,5830µg/ml, so it can be concluded that the ethanol extract of water hyacinth leaves is cytotoxic and has anticancer properties. The test compound is toxic if the LC50 is less than 1000 µg/ml. Keywords : Hyacinth Leaf, Phytochemical Screening, Cytotoxicity Test, LC50
Produksi Protein Sel Tunggal dari Kultur Saccharomyces cerevisiae dengan Medium Limbah Cair Tahu Milwani Harahap; Yayuk Putri Rahayu; Minda Sari Lubis; Rafita Yuniarti
Vitalitas Medis : Jurnal Kesehatan dan Kedokteran Vol. 1 No. 2 (2024): April : Vitalitas Medis : Jurnal Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/vimed.v1i2.151

Abstract

Single Cell Protein (PST) is the term used for proteins originating from microbes such as fungi, algae, yeast and bacteria. Tofu liquid waste is an alternative medium that has the easiest source of carbohydrates, so it has the potential for the growth of Saccharomyces cerevisiae. The aim of this research is to determine whether tofu liquid waste can produce single cell protein from S. cervisiae culture and to determine differences in protein production with the addition of nutrients to the fermentation medium. The research method used is experimental research. The independent variables are MFLT1 medium (Tofu Liquid Waste Fermentation Medium with the addition of KH2PO4 nutrients and sugar) and MFLT2 medium (Tofu Liquid Waste Fermentation Medium with the addition of KH2PO4 nutrients; (NH4)2SO4 and sugar); as well as fermentation time on days 0, 2, 4 and 6. The dependent variables are analysis of protein content, cell dry weight, glucose content, pH and temperature. The data from this research were analyzed statistically using the two way Anova method. The results of this research obtained the highest protein content in MFLT2 medium at 0.52% (day 2); cell dry weight 0.49 grams; glucose level 1.3345%; pH 5.0 and temperature 25.5 ⁰C. Meanwhile, in MFLT1 medium, the highest protein content was obtained at 0.35% (4th day); cell dry weight 0.363 grams; glucose level 1.3342%; pH 4.7 and temperature 28.8 ⁰C. From the research that has been carried out, it can be concluded that tofu liquid waste can produce single cell protein from S. cerevisiae cultures and there are differences in protein production results with the addition of nutrients to the medium, where the results of the protein content in the MFLT2 medium are higher than the protein content in the MFLT1 medium.
Produksi Protein Sel Tunggal dari Kultur Bacillus cereus dengan Medium Limbah Cair Tahu Basaniah Basaniah; Yayuk Putri Rahayu; Haris Munandar Nasution; Gabena Indrayani Dalimunthe
Vitalitas Medis : Jurnal Kesehatan dan Kedokteran Vol. 1 No. 2 (2024): April : Vitalitas Medis : Jurnal Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/vimed.v1i2.152

Abstract

Single Cell Protein (PST) is the term used for proteins derived from microbes such as fungi, yeasts and bacteria. One way to deal with the abundance of tofu liquid waste is by using microbiological methods, namely utilizing tofu liquid waste as a substrate for the growth of Bacillus cereus microbial growth for single cell protein production. The purpose of this study was to determine whether tofu liquid waste could produce single cell protein from B. cereus culture and to determine the difference in protein production by adding nutrients to the fermentation medium. The research method used was experimental research, with the independent variables being MFLT1 fermentation medium (Tofu Liquid Waste Fermentation Medium with the addition of KH2PO4 nutrients and sugar) and MFLT2 medium (Tofu Liquid Waste Fermentation Medium with the addition of nutrients KH2PO4; (NH4)2SO4 and sugar); and the length of fermentation on days 0, 2, 4 and 6. While the dependent variables were the analysis of protein content, cell dry weight, glucose content, pH and temperature. The result data in this study were statistically analyzed using the two way Anova method. The results of this study showed that the highest protein content was obtained in MFLT2 medium, namely 0.73% (day 4); cell dry weight 0.253 g; glucose level 1.3339%; pH 3.9 and temperature 29.2oC. Meanwhile, in MFLT1 medium, the highest protein content was 0.46% (4th day); cell dry weight 0.286 g; glucose level 1.3342%; pH 3.9 and temperature 28.8oC. From the research that has been carried out, it can be concluded that tofu liquid waste can produce single cell protein from B. cereus culture and there are differences in protein production results with the addition of nutrients, where the protein content in MFLT2 medium is higher than protein content in MFLT1 medium.
Co-Authors Anny Sartika Daulay Basaniah Basaniah D.Elysa Putri Mambang Daeng Elysa Putri Mambang Gabena Indrayani Dalimunthe Haris Munandar Nasution Lubis, Minda Sari Milwani Harahap Rafita Yuniarti Retno Sekarini Sekarini, Retno Titin Agustina