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All Journal Jurnal Riset Sains dan Kimia Terapan Makara Journal of Science
Angelina, Helzi
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Chemistry

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Penentuan Kadar Formaldehida pada Baju Bayi dengan Metode Ekstraksi Air menggunakan Spektrofotometri Uv-Vis Sesuai SNI ISO 14184-1:2015 Retnaningsih, Evi; Angelina, Helzi
Jurnal Riset Sains dan Kimia Terapan Vol. 10 No. 1 (2024): Jurnal Riset Sains dan Kimia Terapan, Volume 10 Nomor 1, Juni 2024
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.101.01

Abstract

Abstrak Pakaian bayi, yang bersentuhan langsung dengan kulit, digunakan untuk bayi dari kelahiran hingga usia 36 bulan, merupakan salah satu faktor yang dapat menimbulkan risiko penyakit seperti iritasi kulit atau ruam pada kulit akibat bahan pakaian tersebut. Salah satu bahan kimia berbahaya yang terdapat dalam pakaian bayi adalah Formaldehida. Formaldehida merupakan alergen kimia yang dapat menyebabkan radang kulit akibat kontak dengan zat yang memicu reaksi alergi pada kulit merupakan. Oleh karena itu, penting untuk menguji kadar formaldehida pada pakaian bayi menggunakan spektrofotometer UV-Vis sesuai dengan SNI ISO 14184-1:2015 untuk menilai keamanan pakaian bayi sebelum dipasarkan. SNI ISO 14184-1:2015 adalah metode standar untuk menentukan jumlah formaldehida dalam tekstil. Metode instrumental yang menjadi salah satu alat yang sering digunakan adalah metode Spektrofotometri dengan menggunakan alat Spektofotometri UV-Vis. Hasil pengujian menunjukkan bahwa sampel pakaian bayi dengan kode KN mengandung formaldehida sebanyak 22,42 mg/kg, melebihi batas aman 16 mg/kg menurut Standar Nasional Indonesia (SNI) 14184-1:2015. Dengan demikian, sampel pakaian bayi dengan kode KN tidak aman untuk dipasarkan. Kata kunci: formaldehida, pakaian bayi, spektrofotometri UV-Vis Abstract Baby clothing, which is in direct contact with the skin, used for babies from birth to 36 months of age, is one of the factors that can increase the risk of diseases such as skin irritation or rashes on the skin due to the clothing material. Formaldehyde is a chemical allergen that can cause skin inflammation due to contact with a substance that triggers an allergic reaction on the skin. Therefore, it is important to test the levels of formaldehyde in baby clothing using UV-Vis spectroscopes in accordance with SNI ISO 14184-1:2015 to assess the safety of baby clothes prior to marketing. SNI ISO 14184-1:2015 is a standard method for determining the amount of formaldehyde in textiles. The Spektrofotometer (Spectrophotometer) method, which uses Spectrophotometry UV-Vis, is a commonly used instrumental method. The test results show that samples of baby clothing with a KN code contain 22.42 mg/kg of formalde, exceeding the safe limit of 16mg/kg according to the Indonesian National Standard (SNI) 14184.1:2015. Thus, samples of baby clothes with KN code are not safe to market. Keyword: baby clothes, formaldehyde, spectrophotometry UV-Vis
Detection Method for Escherichia coli Using Real-Time Polymerase Chain Reaction Targeting the yhaV Gene Nurjayadi, Muktiningsih; Fitriyanti, Anisa; Musie, Royna Rahma; Putri, Gusti Angieta; Azizah, Puan Aqila; Angelina, Helzi; Grace, Grace; Sihombing, Ananda Indah Putri; Setiawan, Agus; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Fatimah, Siti; Berkahingrum, Ayu; Kartika, Irma Ratna; Kurniadewi, Fera; Saamia, Vira; Chen, Shyi-Tien; Aboemolak, Bassam; Enshasy, Hesham Ali El
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

Escherichia coli is a foodborne pathogenic bacterium that can cause diarrhea, while yhaV is a virulence-associated gene linked to the toxin–antitoxin system in E. coli. This study was aimed at evaluating the confirmation, specificity, and sensitivity of a yhaV gene primer using real-time polymerase chain reaction. The yhaV-targeting PCR successfully amplified a DNA fragment with an amplicon length of 207 bp (base pairs) under an annealing temperature optimized to a range of 54 °C to 62 °C via gradient PCR. The PCR using the primer pair produced a consistent Ct (cycle threshold) of 14.14 ± 0.05 and showed a single peak in the melting curve at a Tm (melting temperature) of 83.67 °C ± 0.02. The specificity test indicated that the yhaV primer effectively distinguished E. coli from nontarget bacteria on the basis of differences in Ct and Tm values. The sensitivity analysis showed that the PCR directed toward the primer pair successfully detected E. coli at a minimum concentration of 2.24 pg/µL, with a Ct value of 29.93 and a detection limit of 31.5 × 102 CFU. These results suggest that yhaV-based real-time PCR quickly and accurately identifies E. coli. Primer designs that target yhaV have the potential to be developed as components of a rapid, specific, and sensitive kit for detecting E. coli in food samples.
Co-Authors Aboemolak, Bassam Agus Setiawan Azizah, Puan Aqila Berkahingrum, Ayu Chen, Shyi-Tien Enshasy, Hesham Ali El Fitriyanti, Anisa Grace, Grace Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna Kurniadewi, Fera Muktiningsih Nurjayadi Musie, Royna Rahma Putri, Gladys Indira Putri, Gusti Angieta Retnaningsih, Evi Saamia, Vira Sihombing, Ananda Indah Putri Siti Fatimah