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All Journal Jurnal Perlindungan Tanaman Indonesia Kultivasi
Rahman, Ayyub Ar
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Agriculture, Biological Sciences & Forestry

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Phytoplasma Associated with White-backed Planthopper on Rice Plants in Sidrap Regency, South Sulawesi Abbas, Saipul; Djaya, Ernawati; Najamuddin, Erwin; Sebayang, Amelia; Rahman, Ayyub Ar; Aminah, Aminah; Hasbi, Hasbi; Sipi, Surianto; Ridwan, Nur Fathurahman; Ismayanti, Rini; Ibrahim, Elisurya
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.84736

Abstract

South Sulawesi is one of the largest rice production centers in Indonesia. Several important diseases of rice plants, such as those caused by viruses and phytoplasmas, can be transmitted by insect vectors, especially leafhoppers and stem plant. Symptoms of diseases caused by viruses and phytoplasmas are quite diverse but visually similar and difficult to distinguish. This study aims to analyze the presence of phytoplasma associated with white-backed planthopper which are commonly found in rice plantations.  The research method used is by conducting surveys and explorations of insect samples in six villages in Sidrap District. White-back planthoppers found on rice plantations showing symptoms of yellowing and stunted leaves were sampled for further analysis, including total DNA isolation of insects, standard PCR amplification for insect and Nested-PCR for phytoplasma identification, gene sequencing for both amplicons, and nucleotide analysis using BLAST method and Mega X program. The PCR with CO1 primer successfully amplified a 700 bp amplicon from insects, whereas nested-PCR using fP1/rP7 primers followed by m23SR/R16F2n amplified phytoplasma supposedly around 1800 bp and 1250 bp of 16S RNA gene, respectively. The DNA sequencing analysis results indicate that the insect samples were identified as 83% Sogatella vibix species based on homology percentage analysis using BLAST and Mega X Program. As for the phytoplasma, it leans more towards the 16SrI group or Candidatus phytoplasma asteris (Aster yellows phytoplasma) with a homology percentage of 99%.
Maize hybrids’ genetic variability based on qualitative and quantitative traits Priyanto, Slamet Bambang; Herawati, Herawati; Suwarti, Suwarti; Rahman, Ayyub Ar; Andayani, Nining Nurini; Fattah, Abdul; Azrai, Muhammad
Kultivasi Vol 23, No 3 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i3.53166

Abstract

Genetic variability was a prerequisite to doing a plant breeding program. A broad genetic variability allows plant breeders to select a desired genotype. This research aims to assess the maize hybrid's genetic variability based on qualitative and quantitative traits. This research was conducted in the Bone district, south Sulawesi, from November 2022 to March 2023. Fifteen maize hybrids were arranged in a randomized complete block design with three replications. The variables observed are qualitative and quantitative traits. Principal component (PCA) and cluster analyses assessed the genetic variability. The result indicated that based on a loading factor greater than 0.70, the qualitative traits such as intensity of green color, anthocyanin coloration of brace roots, length of lateral branch, intensity anthocyanin coloration of silk, and degree of zigzag displayed high variability. quantitative like days to anthesis, days to silk, leaf length, 1000 seeds weight, yield, ear diameter, number of row seeds per ear, ear height, ear length, and number of seeds per row also exhibit high variability. Cluster analysis shows a broad genetic variability on qualitative and quantitative traits demonstrated by Euclidean levels 6.68-10.93 and 3.43-5.08, respectively, and generated the dendrogram that divides genotypes into four main clusters for qualitative and five for quantitative traits.
Co-Authors Abdul Fattah Amelia Sebayang Aminah Aminah Djaya, Ernawati Hasbi Herawati Herawati Ibrahim, Elisurya Ismayanti, Rini Muhammad Azrai, Muhammad Najamuddin, Erwin Nining Nurini Andayani, Nining Nurini Priyanto, Slamet Bambang Ridwan, Nur Fathurahman Saipul Abbas sipi, Surianto Suwarti Suwarti