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All Journal Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
sipi, Surianto
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Agriculture, Biological Sciences & Forestry

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DECREASE IN PRODUCTION AND QUALITY OF GRAIN DUE TO NECK BLAST DISEASE IN SOME LOWLAND RICE VARIETIES Subiadi, Subiadi; sipi, Surianto; Basundari, Fransiska Renita Anon
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 19, No 1 (2019): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.11974-81

Abstract

Decrease in production and quality of grain due to neck blast disease in some lowland rice varieties. Neck blast disease of rice causes a decrease in yields both in quantity and quality. The study was conducted to see the effect of neck blast disease on the characteristics of grain and yields of several high yielding varieties of lowland rice. The varieties used were Ciherang, Inpari 4, Inpari 7, Inpari 8, and Inpari 9. The parameters observed were the incidence and severity of neck blast disease, the ratio of the size of the seeds of healthy and sick panicles and the estimated production. The analysis showed that all varieties planted were infected by neck blast disease, but based on Disease Incident rate in the Necks, Ciherang and Inpari 8 varieties reacted susceptible to neck blast disease, Inpari 7 and 9 were moderate and Inpari 4 was resistant to neck blast disease.  The quality of panicle grain infected by neck blast disease has decreased the size of grain width and was significantly different from grain of healthy panicles in all varieties. The highest estimated productivity was Inpari 4 variety of 10,378 kg/ha which experienced a decrease in production by 35.86% and the lowest was Ciherang variety of 8,367 kg/ha which experienced a decrease in production by 48.37% when they were infected by neck blast disease.
Phytoplasma Associated with White-backed Planthopper on Rice Plants in Sidrap Regency, South Sulawesi Abbas, Saipul; Djaya, Ernawati; Najamuddin, Erwin; Sebayang, Amelia; Rahman, Ayyub Ar; Aminah, Aminah; Hasbi, Hasbi; Sipi, Surianto; Ridwan, Nur Fathurahman; Ismayanti, Rini; Ibrahim, Elisurya
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.84736

Abstract

South Sulawesi is one of the largest rice production centers in Indonesia. Several important diseases of rice plants, such as those caused by viruses and phytoplasmas, can be transmitted by insect vectors, especially leafhoppers and stem plant. Symptoms of diseases caused by viruses and phytoplasmas are quite diverse but visually similar and difficult to distinguish. This study aims to analyze the presence of phytoplasma associated with white-backed planthopper which are commonly found in rice plantations.  The research method used is by conducting surveys and explorations of insect samples in six villages in Sidrap District. White-back planthoppers found on rice plantations showing symptoms of yellowing and stunted leaves were sampled for further analysis, including total DNA isolation of insects, standard PCR amplification for insect and Nested-PCR for phytoplasma identification, gene sequencing for both amplicons, and nucleotide analysis using BLAST method and Mega X program. The PCR with CO1 primer successfully amplified a 700 bp amplicon from insects, whereas nested-PCR using fP1/rP7 primers followed by m23SR/R16F2n amplified phytoplasma supposedly around 1800 bp and 1250 bp of 16S RNA gene, respectively. The DNA sequencing analysis results indicate that the insect samples were identified as 83% Sogatella vibix species based on homology percentage analysis using BLAST and Mega X Program. As for the phytoplasma, it leans more towards the 16SrI group or Candidatus phytoplasma asteris (Aster yellows phytoplasma) with a homology percentage of 99%.
Co-Authors Amelia Sebayang Aminah Aminah Djaya, Ernawati Fransiska Renita Anon Basundari, Fransiska Renita Anon Hasbi Ibrahim, Elisurya Ismayanti, Rini Najamuddin, Erwin Rahman, Ayyub Ar Ridwan, Nur Fathurahman Saipul Abbas Subiadi Subiadi