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Molecular Detections and Resistance Response of Six Rice Varieties to Tungroviruses from South Sulawesi Saipul Abbas; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47355

Abstract

The suspected rice virus is found in the field, namely the tungrovirus which is transmitted by green leafhoppers (Nephotettix virescens). The study aimed to detect the tungrovirus molecularly and examine the resistance response of six rice varieties from the transmission of tungrovirus samples from South Sulawesi on a greenhouse scale. Based on the results of molecular detection with RTSV PCR of the double infected sample with DNA bands 1115 bp and RTBV of around 430 bp, Sidrap, and Maros samples were infected by 430 bp size RTBV, while Wajo sample was not detected by both viruses. The results of RTBV sequence analysis showed that the grouping of Sidrap was still one group with Maros and Pinrang samples and different from the group of samples from Malaysia, Thailand, and Philippines. While the grouping of RTSV shows that Pinrang samples are still one group with samples from Bali, Subang, and different from those of the Philippines, India, and Malaysia. The results of transmission in the greenhouse on six rice varieties (TN1, Ciherang, Mekongga, Tukad Unda, Inpari 36, Inpari 37) showed different plant resistance responses such as susceptible, moderately resistant, and resistant reactions based on the amount of disease intensity caused. Varieties that are classified as susceptible are TN1 and Ciherang varieties, moderately resistant, namely Mekongga and Tukad Unda varieties, and resistant varieties namely Inpari 36 and Inpari 37 varieties.
Population Fluctuations of Scirpophaga innotata and Nilaparvata lugens In Various Varieties and Growing Age of Rice Plants Saipul Abbas; Itji Diana Daud; Sri Nur Aminah Ngatimin
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4645

Abstract

Stem borer (Scirpophaga innotata) and brown planthopper (Nilaparvata lugens) are known as important pests in rice plants that attack plants from the nursery until just before harvest. Efforts to introduce new high-yielding varieties need to be carried out to provide farmers with references to varieties that are resistant or not to pest populations in the field. This study aims to look at the population levels of stem borer and brown planthopper pests at different planting ages for several test varieties. The method used was a randomized block design with 6 varietal treatments (IR14, IR15, IR16, IR18, IR19, IR20) and 3 tests with sampling intervals every week until 77 days after planting (HST). Data were analyzed using the Analysis of Variance (Anova) method and the Least Significant Difference Test (LSD). The results showed that the highest population of stem borers was found in plants aged 63 HST, namely 8 individuals/clump, while the lowest average population was found in plants aged 49 HST, namely 1 individual/clump. While the highest brown planthopper population was found at 77 HST, namely 10 individuals/clump, for the lowest population average at 63 HST, namely 4 individuals/clump. Populations of stem borer and brown planthopper in fact preferred rice variety IR14 with the highest population, while rice variety IR18 had the lowest population.
Pelatihan Pembuatan CendawanTrichoderma sp. dalam Mengendalikan Penyakit Akar Putih pada Perkebunan Karet di Desa Binturu, Kalimantan Selatan Muslimin Sepe; Helda Orbani Rosa; Salamiah; Saipul Abbas; Yusriadi Marsuni; Muhammad Indar Pramudi; Dewi Fitriyanti; Devaliana Catria Fikasari; Vera Trichoderma
Jalujur: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2023): Desember
Publisher : UIN Antasari Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18592/jalujur.v2i2.11094

Abstract

Penyakit penting yang menyerang tanaman karet yaitu cendawan akar putih, kering alur sadap, gugur daun Corynospora, Colletotrichum dan Oidium. Pohon karet yang terserang penyakit akan mengalami pengguguran daun sehingga pohon karet enggan mengeluarkan getahnya yang membuat penghasilan petani menurun drastis. Produksi dan kualitas karet dapat ditingkatkan dan dipertahankan dengan cara memperhatikan teknik budidaya dan pengendalian organisme pengganggu tumbuhan dengan tepat. Salah satu alternatif menekan populasi OPT dengan memanfaatkan mikroorganisme berguna seperti cendawan Trichoderma sp. Metode PkM yaitu pemberdayaan partisipatif berupa penyuluhan, pelatihan dan pendampingan yang melibatkan peran serta mitra yaitu petani Desa Binturu, Kecamatan Kelua, Kabupaten Tabalong secara aktif mulai dari perencanaan, pelaksanaan, monitoring dan evaluasi. Hasil pengabdian kepada masyarakat menunjukkan sikap antusias dari kelompok tani dalam hal pelatihan pembuatan Trichoderma sp. dan cara pengaplikasiannya di Desa Binturu Kecamatan Kelua, Kabupaten Tabalong, Kalimantan Selatan.
Isolation And Characterization Of Phosphate Solving Bacteria From Swamp Soil With High Levels Of Acidity Galang Indra Jaya; Hidayah Utami, Sri Nuryani; Widada, Jaka; Annisa Yusuf, Wahida; Abbas, Saipul; Fatturahman Ridwan, Nur; Noviyanto, Amir
Jurnal Pertanian Vol. 14 No. 2 (2023): OKTOBER
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jp.v14i2.9932

Abstract

Phosphate solubilizing bacteria (PSB) are one of the microbes that play an important role in soil and plant cycles. Phosphate (P) is a very important macronutrient for plants. In soil, most of the P element is found to be unavailable to plants because it is fixed by Ca, Al or Fe. This research aims to identify BPF in acid soil which has the potential to dissolve phosphate elements. The method used in this research is the isolation of BPF from acid soil using the National Botanical Research Institute's Phosphate (NBRIP) medium, phosphate dissolution index test and UV Visual. Soils from overflow type C (TLC) swamps have higher diversity compared to TLB soils. The abundance of BPF in TLC soil was higher (5,4 107 CFU per gram) compared to soil from overflow zone B (TLB) (2,9 107 CFU per gram) because TLC soil had a lower acidity level than TLB. There were 55 BPF isolates obtained from these two types of soil. Two isolates (TLB1 and TLB2) had a better phosphate solubilization index and all potential isolates that were extracted and subjected to gDNA amplification showed a DNA band at 1330-1500 bp.
Phytoplasma Associated with White-backed Planthopper on Rice Plants in Sidrap Regency, South Sulawesi Abbas, Saipul; Djaya, Ernawati; Najamuddin, Erwin; Sebayang, Amelia; Rahman, Ayyub Ar; Aminah, Aminah; Hasbi, Hasbi; Sipi, Surianto; Ridwan, Nur Fathurahman; Ismayanti, Rini; Ibrahim, Elisurya
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.84736

Abstract

South Sulawesi is one of the largest rice production centers in Indonesia. Several important diseases of rice plants, such as those caused by viruses and phytoplasmas, can be transmitted by insect vectors, especially leafhoppers and stem plant. Symptoms of diseases caused by viruses and phytoplasmas are quite diverse but visually similar and difficult to distinguish. This study aims to analyze the presence of phytoplasma associated with white-backed planthopper which are commonly found in rice plantations.  The research method used is by conducting surveys and explorations of insect samples in six villages in Sidrap District. White-back planthoppers found on rice plantations showing symptoms of yellowing and stunted leaves were sampled for further analysis, including total DNA isolation of insects, standard PCR amplification for insect and Nested-PCR for phytoplasma identification, gene sequencing for both amplicons, and nucleotide analysis using BLAST method and Mega X program. The PCR with CO1 primer successfully amplified a 700 bp amplicon from insects, whereas nested-PCR using fP1/rP7 primers followed by m23SR/R16F2n amplified phytoplasma supposedly around 1800 bp and 1250 bp of 16S RNA gene, respectively. The DNA sequencing analysis results indicate that the insect samples were identified as 83% Sogatella vibix species based on homology percentage analysis using BLAST and Mega X Program. As for the phytoplasma, it leans more towards the 16SrI group or Candidatus phytoplasma asteris (Aster yellows phytoplasma) with a homology percentage of 99%.
Protein Profile Analysis of Tungro and Dwarf Virus-Infected Rice Plants using SDS-PAGE Saipul Abbas
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6120

Abstract

Viruses in rice plants are one of the causes of decreased harvest yields and rice farming productivity. This study aims to analyze the protein profile of viruses in rice plants infected with tungro and dwarf viruses using the SDS-PAGE method. The study includes six plant samples, including tungro-infected plants from Pinrang and Sidrap districts, as well as dwarf virus-infected plants from Sidrap and Wajo districts. Two healthy plant samples were used as a comparison. The SDS-PAGE technique was employed for the separation and analysis of proteins. The protein profile results in tungro-infected plants showed a protein band pattern between 31-116 kDa. Meanwhile, in dwarf virus-infected plants, a protein band pattern between 31-205 kDa was detected. These results indicate variations in protein expression between viruses and significant differences in protein profiles between tungro and dwarf virus-infected plants compared to healthy plants. Therefore, it is expected that this information can aid in designing more effective strategies for controlling virus infections in rice plants.
Kombinasi Pemanfaatan RUBUHA dan Ekstrak Tanaman Tegari untuk Pengendalian Hama Tikus di Lahan Pertanian Pramudi, Muhammad Indar; Marsuni, Yusriadi; Salamiah, Salamiah; Rosa, Helda Orbani; Fitriyanti, Dewi; Abbas, Saipul; Tarigan, Divayona Begidir Br Tarigan; Magfirah, Asmaul; Muhammad Redho
Jurnal Pengabdian Masyarakat Isei Vol. 2 No. 2 (2024): Jurnal Pengabdian Masyarakat Isei
Publisher : ISEI Cabang Pekanbaru, Koordinator Provinsi Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46750/abdimasisei.v2i2.269

Abstract

Program pengabdian masyarakat ini bertujuan untuk mengembangkan solusi ramah lingkungan dalam pengendalian hama tikus menggunakan dua pendekatan inovatif: pemasangan Rumah Burung Hantu (RUBUHA) dan pemanfaatan ekstrak tanaman tegari (Dianella sp.) sebagai pengusir alami tikus. Pemasangan RUBUHA menyediakan habitat bagi burung hantu sebagai predator alami tikus, sedangkan ekstrak tanaman tegari digunakan sebagai atraktan untuk tikus. Program ini dilakukan di Desa Bentok Darat, Kalimantan Selatan, dengan melibatkan kelompok tani sebagai mitra. Hasil program menunjukkan bahwa kombinasi kedua metode ini efektif mengurangi populasi tikus hingga 70%, sekaligus meningkatkan kesadaran petani tentang pengendalian hama tikus yang berkelanjutan.
Optimalisasi Pengendalian Tikus melalui Pemasangan RUBUHA di Desa Bentok Darat, Kalimantan Selatan Muhammad Indar Pramudi; Yusriadi Marsuni; Saipul Abbas; Helda Orbani Rosa; Dewi Fitriyanti
PaKMas: Jurnal Pengabdian Kepada Masyarakat Vol 5 No 1 (2025): Mei 2025
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/pakmas.v5i1.3306

Abstract

Rodent pests (Rattus spp.) are one of the main threats to agricultural productivity in Indonesia, including in Bentok Darat Village, South Kalimantan. The damage caused by these pests not only reduces crop yields but also increases farmers' dependence on chemical pesticides, which pose environmental risks. To address this issue, effective and environmentally friendly pest control methods are needed. One approach employed is the installation of Owl Houses (RUBUHA) as habitats for barn owls, natural predators of rodents. This community service program aims to optimize rodent pest control by installing RUBUHA on agricultural land. The activities involve students and the Alam Subur farmer group, including training on RUBUHA installation, facility maintenance, and monitoring its effectiveness in reducing rodent populations. Observations showed that the installation of RUBUHA significantly reduced rodent attacks on agricultural land. Additionally, this method successfully increased crop productivity by up to 30% and decreased the use of chemical pesticides. In conclusion, RUBUHA installation is an innovative and sustainable solution for rodent pest control, supporting environmentally friendly agricultural practices while enhancing farmers' welfare.
PKM OPTIMALISASI PERAN BIOKONTROL DALAM BUDIDAYA HORTIKULTURA - PELATIHAN UNTUK PETANI DAN PENYULUH DI LAHAN BASAH Aidawati, Noor; Sepe, Muslimin; Liestiany, Elly; Abbas, Saipul; Matondang, Putri
BAKIRA: Jurnal Pengabdian Kepada Masyarakat Vol 6 No 1 (2025): BAKIRA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bakira.2025.6.1.13-21

Abstract

This Community Service Program (PkM) aims to enhance the understanding and skills of farmers and agricultural extension workers in optimizing the role of biocontrol agents in horticultural cultivation on wetland areas. The activities were carried out through training sessions, field practices, and technical assistance. The training materials covered the identification of various types of biocontrol agents, application techniques, and their benefits in reducing the use of chemical pesticides. Evaluation results indicated a significant increase in participants’ knowledge levels, as well as a positive shift in attitudes toward the adoption of more environmentally friendly biological control methods. Moreover, the application of biocontrol agents in demonstration plots showed healthier plant growth with minimal pest attacks. This program is expected to serve as an initial step towards promoting sustainable horticultural cultivation practices in wetland areas.
Mapping Urban Green Space Fragmentation using Drone Technology in Banjarbaru City Rizali, Akhmad; Abbas, Saipul; Marsuni, Yusriadi; Sari, Yulia Padma; Farah, Nurin Nisa; Noorkomalasari, Noorkomalasari; Badaruddin, Badaruddin
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8920

Abstract

The rapid urban growth in Banjarbaru City has significantly impacted the quality and quantity of green open spaces. Fragmentation of green spaces poses a major challenge in maintaining the city's ecological balance, particularly amidst high urbanization rates. This study aims to analyze the distribution and fragmentation level of green spaces across five sub-districts in Banjarbaru City using drone technology and spatial approaches. Data were collected through high-resolution aerial mapping and spatially analyzed to identify fragmentation patterns and compare them with the 2024-2043 Spatial Planning (RTRW) of Banjarbaru City. The results show that the highest fragmentation occurs in the Landasan Ulin and South Banjarbaru sub-districts, characterized by small patches and low core vegetation areas. Meanwhile, North Banjarbaru, Cempaka, and Liang Anggang sub-districts still retain extensive and connected green spaces. A comparison with the RTRW reveals a deviation between the planned and actual conditions, particularly in areas designated to be preserved as green open spaces.