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All Journal Jurnal Fitopatologi Indonesia agriTECH Jurnal Perlindungan Tanaman Indonesia
Amelia Sebayang
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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The The Effect of Gamma Rays on Local Chilli Infected to Pepper Yellow Leaf Curl Virus Infection (Begomovirus) Tarigan, Rasiska; Hanafia, Diana Sofia; Sinuraya, Mariati; Barus, Susilawati; Marpaung, Agustina E; Murtiningsih, Rini; Sebayang, Amelia
Jurnal Fitopatologi Indonesia Vol 19 No 2 (2023): Maret 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.2.74-82

Abstract

Cabai merupakan salah satu komoditas hortikultura bernilai ekonomi tinggi di Indonesia. Cabai lokal Karo berbatang ungu merupakan cabai yang memiliki keunggulan produksi dan pertumbuhan vegetatif tinggi, namun sangat rentan terhadap penyakit virus keriting kuning oleh begomovirus. Salah satu upaya pengendalian begomovirus ialah dengan perakitan genotipe baru yang tahan melalui induksi iradiasi sinar gamma. Penelitian bertujuan mengkaji pengaruh iradiasi sinar gamma pada benih cabai lokal Karo berbatang ungu terinfeksi begomovirus untuk mendapatkan calon genotipe mutan yang tahan. Benih dari tanaman cabai terinfeksi begomovirus diberi perlakuan iradiasi sinar gamma dengan taraf dosis 150, 200, dan 250 Gy untuk mendapatkan tanaman mutan 1 (M1). Benih sehat dan benih berasal dari tanaman sakit tanpa perlakuan digunakan sebagai tanaman kontrol. Parameter yang diamati meliputi insidensi dan keparahan penyakit, AUDPC dan deteksi begomovirus pada tanaman M1 dan benih generasi kedua (M2). Perlakuan iradiasi sinar gamma taraf dosis 150 Gy pada benih cabai terinfeksi begomovirus menunjukkan rata-rata insidensi, keparahan penyakit, dan AUDPC paling rendah dibandingkan dengan perlakuan lainnya pada tanaman mutan M1 dan M2. Berdasarkan deteksi dengan PCR pada tanaman mutan M2, dari perlakuan dosis 150 Gy didapatkan 7 calon genotipe tahan begomovirus terbanyak di antara perlakuan lainnya.
Phytoplasma Associated with White-backed Planthopper on Rice Plants in Sidrap Regency, South Sulawesi Abbas, Saipul; Djaya, Ernawati; Najamuddin, Erwin; Sebayang, Amelia; Rahman, Ayyub Ar; Aminah, Aminah; Hasbi, Hasbi; Sipi, Surianto; Ridwan, Nur Fathurahman; Ismayanti, Rini; Ibrahim, Elisurya
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.84736

Abstract

South Sulawesi is one of the largest rice production centers in Indonesia. Several important diseases of rice plants, such as those caused by viruses and phytoplasmas, can be transmitted by insect vectors, especially leafhoppers and stem plant. Symptoms of diseases caused by viruses and phytoplasmas are quite diverse but visually similar and difficult to distinguish. This study aims to analyze the presence of phytoplasma associated with white-backed planthopper which are commonly found in rice plantations.  The research method used is by conducting surveys and explorations of insect samples in six villages in Sidrap District. White-back planthoppers found on rice plantations showing symptoms of yellowing and stunted leaves were sampled for further analysis, including total DNA isolation of insects, standard PCR amplification for insect and Nested-PCR for phytoplasma identification, gene sequencing for both amplicons, and nucleotide analysis using BLAST method and Mega X program. The PCR with CO1 primer successfully amplified a 700 bp amplicon from insects, whereas nested-PCR using fP1/rP7 primers followed by m23SR/R16F2n amplified phytoplasma supposedly around 1800 bp and 1250 bp of 16S RNA gene, respectively. The DNA sequencing analysis results indicate that the insect samples were identified as 83% Sogatella vibix species based on homology percentage analysis using BLAST and Mega X Program. As for the phytoplasma, it leans more towards the 16SrI group or Candidatus phytoplasma asteris (Aster yellows phytoplasma) with a homology percentage of 99%.
Application of Eco-Enzyme Solution as A Multipurpose Liquid Fertilizer to Enhance Performance of Sorghum Varieties Irmansyah, T.; Simangunsong, Alwan Alawi; Sebayang, Amelia; Girsang, Setia Sari; Lubis, Dini Sahfitri
agriTECH Vol 44, No 3 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.86048

Abstract

Eco-enzyme, derived from various household wastes such as vegetables, fruits, or fruit peel, is an organic fertilizer rich in essential nutrients for plant growth. Therefore, this study aimed to investigate the impact of eco-enzyme concentrations on the growth and yield of several sorghum cultivars (Sorghum bicolor (L.) Moench) in Tanjung Sari, Medan Selayang Subdistrict, Medan from June to September 2021. To achieve this objective, a Randomized Block Design (RBD) comprising 2 factors, namely eco-enzyme concentration (T0:0 mL/L, T1:9.3 mL/L, T2: 18.6 mL/L, and T3: 27.9 mL/L) and sorghum varieties (S1: Super 1, S2: Super 2, S3: Suri 4) was adopted. Eco-enzyme solution consisted of molasses, fruit peels (watermelon, cucumber, and orange), and water. The results showed that the interaction between Suri 4 and a concentration of 27.9 mL/L affected sorghum production. Each ecoenzyme and the varieties affected stem diameter (24.93 mm and 22.66 mm), flowering time (59.19 DAP and 56.00 DAP), as well as wet (631.03 g and 410.20 g) and dry stove weights (315.52 g and 205.10 g). Consequently, the application of eco-enzyme at a concentration of 27.9 mL/L enhances both the growth and yield of sorghum.
Co-Authors Agustina E Marpaung Aminah Aminah Barus, Susilawati Djaya, Ernawati Girsang, Setia Sari Hanafia, Diana Sofia Hasbi Ibrahim, Elisurya Ismayanti, Rini Lubis, Dini Sahfitri Mariati Sinuraya Najamuddin, Erwin Rahman, Ayyub Ar Rasiska Tarigan Ridwan, Nur Fathurahman Rini Murtiningsih Saipul Abbas Simangunsong, Alwan Alawi sipi, Surianto T. Irmansyah