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EKSTRAK DAUN Avicennia marina UNTUK MENEKAN AKTIFITAS BAKTERI Vibrio spp. YANG DIISOLASI DARI KEPITING BAKAU (Scylla serrata) SECARA IN VITRO Mohammad Fadnan Akhmadi; Ery Gusman; Karmila Dewi
Jurnal Harpodon Borneo Vol 11, No 2 (2018): Volume 11 No.2 Oktober 2018
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (608.409 KB) | DOI: 10.35334/harpodon.v11i2.534

Abstract

ABSTRACTAvicennia marina leaf extract is known to suppress the activity of Vibrio spp. using solvent klorform and methanol. Therefore, the authors conducted a further study using different solvents. This research interest determine the ability of extracts of A. marina in suppressing the activity of Vibrio spp. isolated from mud crab (Scylla serrata). A. Marina leaf take in Pantai Amal Lama, The process of extraction, isolation and testing of clear zone performed at the Laboratory of the Faculty of Fisheries and Marine Sciences, University of Borneo Tarakan. Stages of research are: manufacture A. marina leaf extract, isolation, culture of Vibrio spp., and data analysis. The study consisted of 6 treatments, blank control, negative control, positive control, A. marina leaf extract concentration of 15%, 25% and 35%, A. marina leaf extract in each treatment were significantly different (P 0.05). Diameter of clear zone produced at a concentration of 15% obtained an average value of 12.25 ± 5.37 mm, at a concentration of 25% 24.4 ± 1.41 mm, at a concentration of 35% 30.37 ± 0.95 mm. While the positive control 42.5 ± 0:00 mm. But the blank control and negative controls do not form clear zones. Based on these results it can be concluded that there are indications A. marina leaf extract in suppressing the activity of Vibrio spp. were tested in vitro.Keywords: Leaf of A. marina, Vibrio spp., Mangrove crabs (Scylla serrata),                 in Vitro
PEMANFAATAN BUAH MANGROVE PEDADA (Sonneratia sp) SEBAGAI CAMPURAN PAKAN UNTUK MENINGKATKAN PERTUMBUHAN IKAN MAS Ery Gusman; Jimmy Cahyadi
Jurnal Harpodon Borneo Vol 8, No 2 (2015): Volume 8 No 2 Oktober 2015
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.225 KB) | DOI: 10.35334/harpodon.v8i2.124

Abstract

Feed was the most valueable input cost in aquaculture process (fish and shrimp) and very influenced toward cultivan growth.  Therefore artificial feed from manufacture always facing to the cost problem in providing raw material that always rise.  The purposes of this research is to find out the advanteges from mangrove fruits as alternative raw material for artificial feeding and also to searching the ability of mangrove fruits in artificial feed to increasing the growth of carps fish.  This research was done from April 2015 until May 2015 at aquacultute technology laboratory, Faculty Fishieries and Marine, University of Borneo Tarakan, North Borneo.  The fruits of mangrove that was used is Pedada (Sonneratia sp.).  The design of this research is randomized completely design with 4 treatment that are : A (Control, manufacture artificial feed), B (Pedada 30%), C (Pedada 40%), and D (Pedada 50%).  The high value of Specific Growth Rate is treatment C (Pedada powder with protein concentration 40%) 1.66%, followed by  treatment D (Pedada powder with protein concentration 50%) 1.44%, treatment A (Control) 1.14%, and treatment B (Pedada Powder with protein concentration 30%) 0.84%.  The high value of absolute growth  is in treatment C (Pedada powder with protein concentration 40%) followed by treatment D (Pedada powder with protein concentration 50%), treatment A (Control,manufacture artificial feed), and treatment B (Pedada powder with protein concentration 30%).  The value of Feed Convertion Rate is about 1.05-1.42, more high than manufacture artificial feed (0.99).  the high value of Survival Rate reached by treatment C (80%), followed by treatment B (73.33%), treatment D (70%) and treatment A (53.33%).  Keywords : mangrove fruit, Sonneratia, artificial feed, fish, Common carp
SISTEM PERTAHANAN TUBUH IKAN : RESPON PERTAHANAN ADAPTIF, MAJOR HISTOCOMPATIBILITY COMPLEX (MHC), RESEPTOR SEL T, SITOKIN Ery Gusman
Jurnal Harpodon Borneo Vol 4, No 1 (2011): Volume 4 No 1 April 2011
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.311 KB) | DOI: 10.35334/harpodon.v4i1.63

Abstract

Teleostei Fish, as members of other vertebrates have a body defense system when the body is infected by foreign agents from both types of bacteria, viruses, parasites, and fungi. Fish has two defense systems that was natural defense system (innate immunity) and the adaptive defense system (adaptive immunity). Major Histo Compatibility (MHC) is a group or complex of genes that play a role in recognition and signaling between immune cells. T cell receptor (TCR) is an antigen that presented by MHC molecules, both MHC class I or class II MHC, to T cells and then bound by a receptor located on the surface of T cells Cytokines are soluble protein mediators (sometimes bound in the cell membrane ) which bind to receptors on cells target and trigger, regulate, or inhibit cellular functions. Detailed knowledge about the molecular expression of immune cells in fish can be used as a basic reference for designing the construction of peptide-based vaccines that are immunogenic and specifically identify a variety of viruses and bacteria that often infects fish. Keywords :            Fish, adaptive immunity, Major Histocompatibility Complex (MHC), Reseptor Sel T, Sitokin
Identification of Vibrio isolated from Mangrove Sediment Near to Vannamei Ponds Ery Gusman
Samakia : Jurnal Ilmu Perikanan Vol 10 No 2 (2019): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.234 KB) | DOI: 10.35316/jsapi.v10i2.540

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This study aims to identify the vibrio in mangrove forest areas near to the location of vannamei ponds, especially in sediment substrates. The research is at the Mangunharjo sub-district in the Mayangan District, Probolinggo City, East Java Province. The time of study is august and September 2019. Isolation of bacteria being done with total plate count procedure on Thio Sulfate Citrate Bile Sucrose (TCBS) Agar, with pour plate method. Morphology characterization and the biochemical test was done to identify the bacteria. There are three points of the sampling area, with each area distance from 100 to 500 meters. This location choosing based on purposive sampling method, which main reason is the site must have mangrove with good vegetation and variation. It is transected by 5m x 5m, 100-gram sediment at 30-40 cm depth taken randomly using sediment scoop. Three samples of each sampling area chosen randomly at three different locations, then mixed. The result showed each deposit from different sampling locations having bacteria colony with color are yellow, green, and green blueish. These colony of bacteria in this sediment suspected as v. harveyii, v. alginolyticus, v. fischery dan v. mimicus.
SKRINING FITOKIMIA EKSTRAK BUAH MANGROVE (Sonneratia alba) SEBAGAI BIOENRICHMENT PAKAN ALAMI Artemia salina Jimmy Cahyadi; Gloria Ika Satriani; Ery Gusman; Encik Weliyadi; Sabri Sabri
Jurnal Borneo Saintek Vol 1, No 3 (2018)
Publisher : Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/borneo_saintek.v1i3.932

Abstract

Penelitian mengenai skrining fitokimia terhadap ekstrak buah mangrove pedada(Sonneratia alba) Telah dilakukan. Buah pedada (Sonneratia alba) diperoleh darimangrove pesisir Kota Tarakan, Kalimantan Utara. Data yang dianalisis meliputi kondisisimplisia, rendemen, ekstraksi dan skrining fitokimia pada Artemia salina nonbioenrichment maupun pasca bioenrichment. Prosedur pembuatan simplisia melaluisortasi basah dan kering, analisa rendemen berdasarkan rasio bobot, ekstraksi buahpedada (Sonneratia alba) berdasarkan teknik maserasi menggunakan pelarut etanol96%, dan skrining fitokimia dilakukan melalui pengujian warna menggunakan berbagaipereaksi. Teknik analisa data dilakukan secara kualitatif deskriftif. Hasil penelitianmenyimpulkan bahwa ekstrak pasta kental (Sonneratia alba) positif mengandungalkaloid, flavonoid, tannin, steroid dan tripernoid pada bioenrichment dan senyawasteroid dan tanin negatif pada non bioenrichment.
PENGUJIAN MUTU UDANG WINDU BERDASARKAN TOTAL PLATE COUNT (TPC) BAKTERI Escherichia coli DAN Coliform DI PT. PMMP TARAKAN Hendra Subarka; Gloria Ika Satriani; Ery Gusman
Jurnal Borneo Saintek Vol 1, No 1 (2017)
Publisher : Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/borneo_saintek.v1i1.881

Abstract

Penanganan produk bahan baku perikanan udang windu yang baik menjadi peranan yang sangat penting bagi manusia. Dengan mengikuti prosedur penanganan yang telah ditetapkan oleh BSNI (Badan Standar Nasionalisasi Indonesia) maka, kita dapat terhindar dari penyakit yang disebabkan oleh bakteri patogen yang berbahaya. Secara umum penelitian ini bertujuan untuk menganalisa mutu udang windu berdasarkan pengujian TPC (Total Plate Count), Escherichia coli dan coliform. Penelitian ini bersifat deskriptif dengan mengunakan Angka Paling Memungkinkan (APM) dan mengunakan perhitungan TPC. Sampel yang akan digunkan yaitu udang windu yang di ambil dari sub player (Pos Penampungan) yang berada pada perusahaan PT.PMMP Kota Tarakan. Hasil Penelitian ini menunjukan bahwa semua sampel udang windu yang dilakukan pengujian, tidak adanya cemaran bakteri E.coli atau negatif bakteri E.coli dalam sampel udang yang diuji. Dalam tingkat pengujian TPC semua sampel masih dibawah ambang batas dari SNI 01-2728.1-2006 yang telah ditetapkan. Di dalam segi pencemaran bakteri coliform didapat 2 sampel yang mengandung kontaminasi bakteri coliform yaitu UD 3 dan UD 7. Namun cemaran bakteri coliform tersebut bukan berasal dari spesies bakteri E.coli.