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All Journal Jurnal Gizi dan Pangan
Wan Ishak, Wan Rosli
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Health Professions

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Effectiveness of Cosmos caudatus Extract in Improving Health-Related Parameters among Older Adults with Sarcopenia: A Study Protocol Nazri, Nurul Syahidah; Vanoh, Divya; Murugaiyah, Vikneswaran; Ibrahim, Baharudin; Kannan, Thirumulu Ponnuraj; Wan Ishak, Wan Rosli; Shahar, Suzana
Jurnal Gizi dan Pangan Vol. 19 No. 2 (2024)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2024.19.2.87-89

Abstract

This is a randomized, two-arm, double-blind, placebo-controlled study that will be performed among older adults in Kelantan, Malaysia, for 12 weeks. This study aims to determine the effectiveness of a 12-week Cosmos caudatus (C. caudatus) extract supplementation on dietary intake, cognition, mental health, sleep quality, disability, gut microbiota, physical activity, frailty, metabolites, and protein synthesis pathways among older adults with sarcopenia. This study will involve 64 older adults diagnosed with sarcopenia that will be enrolled and randomly allocated with 1:1 ratio, where 32 subjects required in both intervention and placebo group. The intervention group will receive 500 mg/day of C. caudatus supplementation, while the placebo group will be given 500 mg/day of maltodextrin. Study outcomes including sarcopenia status, dementia, motoric cognitive risk, dietary pattern, anthropometric data, physical fitness, mental health, disability and sleep quality will be measured. Blood will be taken for analysis of protein synthesis pathways (using blood plasma) and untargeted metabolomics at baseline and the 12th week. Fecal sample will be collected from a subsample of 24 subjects for gut microbiota analysis. This study is one of the pioneers randomized controlled trials to evaluate C. caudatus efficacy on various health related outcomes among sarcopenic community-dwelling elderly. This study findings are hoped to have the potential to improve health awareness of ulam or fresh salad consumption, specifically C. caudatus and prove its effectiveness as a nutraceutical product for older adults with sarcopenia. The Australian New Zealand Clinical Trials Registry (ANZCTR) has this trial listed as registered with code (ACTRN12623000046606p).
Effect of Kappaphycus alvarezii and Overripe Banana Sweetener Addition on the Nutritional Composition and Palatability of Cookies Nie, Linlin; Wan Ishak, Wan Rosli; Mohd Kamil, Khadijah; Majahar Ali, Majid Khan
Jurnal Gizi dan Pangan Vol. 19 No. Supp.2 (2024)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2024.19.Supp.2.269-276

Abstract

This study aimed to assess the qualities of butter biscuits made with seaweed flour (Kappaphycus alvarezii, (KA) (0, 4, and 8%) and Overripe Banana Sweetener (ORBS) (0, 50, and 100%) as a partial replacement for wheat flour and table sugar. A.O.A.C. and hedonic methodologies have been used to analyze the nutritional composition, color, and sensory evaluation of butter cookies. The increase in ORBS in cookie formulation resulted in a significant rise in the nutritional qualities of butter cookies, according to the result. Butter cookies with 4% seaweed flour and 100% ORBS had higher total dietary fiber (19.6%) and ash (3.07%) values. Sensory scores for the control (0%) and 4% seaweed flour-incorporated cookies did not differ substantially across all sensory qualities. However, the addition of 4% seaweed flour and 50% ORBS resulted in the highest scores for aroma, flavor, and overall acceptance. In conclusion, substituting 4% seaweed flour for wheat flour and 50% ORBS for table sugar could be an effective combination to make nutritious and tasty butter cookies.
Prebiotic Activity of Plants from Cucurbitaceae Family and In Vitro Fermentation by Gut Microbiota Ab Azid, Saffanah Mohd; Wichienchot, Santad; Wan Ishak, Wan Rosli; Safuan, Sabreena
Jurnal Gizi dan Pangan Vol. 19 No. Supp.2 (2024)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2024.19.Supp.2.342-351

Abstract

This study aims to examine the effect of Freeze-Dried Pumpkin Powder (FDPP), Freeze-Dried Winter Melon Powder (FDWMP), Freeze-Dried Rock Melon Powder (FDRMP), inulin, and D glucose on prebiotic activity score by in vitro fermentation. We also elucidate the changes in bacterial populations through batch culture fermentation using fecal samples from 5 healthy volunteers and In vitro fecal fermentation using batch culture and analyses of Short-Chain Fatty Acids (SCFAs). The growth of Bifidobacterium has significantly increased from 0 (8.90±0.05 log10 cells/mL) and 72h (8.83±0.14 log10 cells/mL) for D glucose and FDWMP (8.75±0.07 log10 cells/mL (0h) and 8.87±0.12 log10 cells/mL (72h)). However, the increase in population was not significant for inulin (9.15±0.06 log10 cells/mL), FDPP (9.04±0.12 log10 cells/mL), and FDRMP (8.67±0.08 log10 cells/mL). The number of Lactobacili significantly increased at 6h for FDPP (9.11±0.07 log10 cells/mL) and 24h for FDWMP (8.88±0.07 log10 cells/mL) and FDRMP (8.80±0.09 log10 cells/mL). Acetic acid was detected in all samples, and the concentration increased in all vessels at any given time except for the FDWMP fermentation, which decreased after 0h and increased after 6h. Overall, FDWMP has increased the probiotic growth of L. plantarum TISTR 1465 and exhibit the highest prebiotic index. As a result, it is suggested that the FDWMP be potentially used as a healthy raw material in developing varieties of functional prebiotic food products.
Predicted Glycaemic Index Values of Rice Prepared with Different Cooking Methods Binti Mohd Khairi, Nur Maisarah; Wan Ishak, Wan Rosli
Jurnal Gizi dan Pangan Vol. 18 No. 2 (2023)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2023.18.2.99-108

Abstract

The current study investigated how the preparation methods impact the nutritional composition and estimated Glycaemic Index (eGI) of the white and brown rice samples. The analysis of proximate and eGI was conducted after the white and brown rice were prepared through these cooking methods: (1) cooking rice without additional coconut oil and not being refrigerated (control); (2) cooking rice without additional coconut oil and being refrigerated (XCOR); (3) cooking rice with additional coconut oil and not being refrigerated (COXR); and (4) cooking rice with additional coconut oil and being refrigerated (COR). The result showed that the COR method recorded the lowest calorie for both white and brown rice (223.93 kcal and 169.90 kcal per 100 g, respectively). Meanwhile, the COR method also recorded the lowest eGI for both white rice (2.31, 6.36, 6.07, 4.55, 3.02, 2.22 nm/min) and brown rice (1.44, 1.92, 1.92, 1.36, 0.66, 0.27 nm/min) at 20, 30, 60, 90, 120, and 180 min, respectively. In conclusion, consuming refrigerated rice that has been cooked with coconut oil can be used as an alternative preparation technique to lower both calorific value and glycaemic index for the preparation of healthier rice meals for health-conscious individuals.
High-Pressure Pre-Treatment of Kappaphycus alvarezii: Effect of Drying Rate on Physicochemical Properties and Antioxidant Activities Ahmad Fahmi Lim, Ahmad Fakhzan Lim; Azhani Amran, Siti; Zulkurnain, Musfirah; Majahar Ali, Majid Khan; Azima, Fauzan; Wan Ishak, Wan Rosli
Jurnal Gizi dan Pangan Vol. 20 No. 1 (2025)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2025.20.1.1-10

Abstract

This study focuses on High-Pressure Pre-Treatment to enhance the seaweed's nutritional value and antioxidant potential, which is highly relevant and aligns with the demand for improved food processing techniques that preserve bioactive compounds. High-Pressure Processing (HPP) was applied at varying levels (0, 200, 400, and 600 MPa) to assess its impact on drying efficiency, physicochemical properties, and antioxidant activities. High-Pressure Processing (HPP) at 200 MPa, 400 MPa, and 600 MPa enhanced the drying performance and antioxidant properties of Kappaphycus alvarezii. The 600 MPa treatment achieved the fastest drying rate and the highest antioxidant capacity, thereby enhancing the seaweed’s functional properties. Moreover, the 600 MPa treatment yielded the highest total phenolic content (50.68±1.51 mg GAE/100 g) and a significant enhancement in total flavonoid content (5.54±0.29 mg QE/100 g). These compounds are crucial for neutralizing free radicals and mitigating oxidative stress. Furthermore, the 600 MPa treatment demonstrated a significant increase in ferric reducing antioxidant power assay (44.58±2.56 mg FeSO4/100 g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (18.86±0.66%) relative to the untreated sample, highlighting the improved antioxidant potential associated with high-pressure treatment. These findings indicate that HPP not only preserves but also enhances the antioxidant properties of Kappaphycus alvarezii, which are vital for its health benefits. Additionally, color analysis revealed significant changes in the lightness and chromaticity of the seaweed post-treatment, suggesting improvements in its visual appeal. This research underscores the capability of HPP technology to improve the drying efficiency of Kappaphycus alvarezii, thereby boosting its antioxidative properties, marketability, and versatility in various applications.
Protective Effects of the Polyphenolic-Rich Fraction of Cornsilk against Oxidative Stress in Streptozotocin-Induced Diabetic Rats Hamzah, Nurraihana; Safuan, Sabreena; Wan Ishak, Wan Rosli
Jurnal Gizi dan Pangan Vol. 18 No. 1 (2023)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2023.18.1.41-50

Abstract

Diabetes Mellitus (DM) has become a significant public health problem worldwide and primarily correlated to hyperglycaemia and abnormal lipid and antioxidant levels. Fruit and vegetable wastes are rich in phenolic compounds thus suitable for antioxidant sources. Cornsilk (CS), a maize cultivar waste, also contains phenolic compounds. The current study investigated the anti-hyperglycemic and antioxidative properties of the Phenolic-Rich Fraction of Cornsilk (PRF-CS) in Streptozotocin (STZ)-induced diabetic rats. Five groups of 30 male Sprague Dawley rats were employed in this study. A sample size of six rats each is placed in five groups: Normal-Control (NC), Diabetic-Control (DC), Diabetic-PRF-CS treated 100 mg/kg (DPRF100) and 200 mg/kg (DPRF200), and Diabetic-Metformin Treated (Dmet) groups. The PRF-CS was administered at 100 and 200 mg/kg doses for 28 consecutive days to the diabetic rats. Treatment with both doses of PRF-CS (DPRF100 and DPRF200) significantly decreased the blood glucose levels of the rats (p<0.05). Additionally, the PRF-treated rats demonstrated significantly decreased (p<0.05) lipid peroxidation (3.60±0.23 and 3.31±0.56 µmol/g, respectively). The hepatic antioxidant enzyme activities of Superoxide Dismutase (SOD) (169.35±4.75 and 175.30±3.69 U/mg, respectively), Catalase (CAT) (1457.51±152.74 and 2011.99±396.96 U/mg), and Glutathione Peroxidase (GSH-Px) (63.43±2.99 and 78.47±4.51 U/mg) were also elevated in contrast to the DC group. Furthermore, the PRF-CS administration improved the histological alterations in the liver tissues of the DPRF100 and DPRF200 rats. In conclusion, PRF-CS treatment exhibited protective effects in the diabetic rat model by decreasing oxidative stress and preserving liver integrity.
Co-Authors Ab Azid, Saffanah Mohd Ahmad Fahmi Lim, Ahmad Fakhzan Lim Azhani Amran, Siti Binti Mohd Khairi, Nur Maisarah Fauzan Azima Hamzah, Nurraihana Ibrahim, Baharudin Kannan, Thirumulu Ponnuraj Majahar Ali, Majid Khan Mohd Kamil, Khadijah Murugaiyah, Vikneswaran Nazri, Nurul Syahidah Nie, Linlin Safuan, Sabreena Santad Wichienchot Shahar, Suzana Vanoh, Divya Zulkurnain, Musfirah