Munawer Pradana
Department of Reproduction, Faculty of Veterinary Medicine, Mandalika University of Education, Pemuda Street Number 59A, Mataram, West Nusa Tenggara 83125

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Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Identification of Gastrointestinal Protozoa of Sumatera Elephant (Elephas maximmus sumatranus) in Lombok Wildlife Park Fernando Jose Immanuel Clinton Situmorang; Kholik Kholik; Candra Dwi Atma; Katty Hendriana Priscilia Riwu; Iwan Doddy Dharmawibawa; Munawer Pradana
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11855

Abstract

The protozoan parasites have been reported to infect Sumatran elephants. Gastrointestinal protozoa could potentially be a factor in the decline in the Sumatran elephant population in Indonesia. This study aims to identify the presence of gastrointestinal protozoa in Sumatran Elephant (Elephas maximus sumatranus) in the Lombok Wildlife Park, North Lombok Regency, Indonesia. This research has used fresh fecal samples from 5 Sumatran elephants. The examination of feces samples using native, sedimentation, and floating methods. The research results have identified the presence of gastrointestinal protozoa in 2 of the 5 Sumatran elephant feces examined. The gastrointestinal protozoa found were Eimeria spp with dimensions of 16.30 x 20.93 μm and 25.21 x 38.49 μm.
Prevalence and Morphological Identification of Eimeria Species on Bali Cattle in Sumbawa District, West Nusa Tenggara Pradana, Munawer; Kurniawan, Muhammad Ahdi’; Berliana, Erprinanda Galuh; ‘Ilmi, Laily Ulya Nurul; Haddar, Mariyam Al; Dharmawibawa, Iwan Doddy
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13315

Abstract

This study aims to determine the prevalence and morphological identification of Eimeria spp. from Bali cattle feces on traditional farms in Sumbawa District. A total of 300 samples were identified and 238 samples were positive, resulting in a prevalence of Eimeria spp. of 79.33%. Based on age category, the highest prevalence of Eimeria spp. was found in cattle aged 6 months-2 years (88.76%; 79/89), followed by cattle aged  <6 months (88%; 88/100) and the lowest prevalence in cattle aged >2 years (63.96%; 71/111). Female Bali cattle had the highest prevalence of Eimeria spp. (80.55%; 145/180) than male Bali cattle (77.50%; 93/120). Based on livestock systems, the highest prevalence of Eimeria spp. was found in semi-intensive (89.15%; 74/83), then extensive (80.76%; 105/130) and the lowest in intensive (67.81%; 59/87). Six different Eimeria species were identified: E. bovis (33.19%), E. auburnensis (21.42%), E. zuernii (15.96%), E. alabamensis (12.18%), E. ellipsoidalis (10.50%), and E. canadensis (6.72%). Preventing and controlling coccidiosis infection in cattle can be done with good hygiene management.