Article Per Year (5 Year)
p-Index From 2020 - 2025
1.152
P-Index
This Author published in this journals
All Journal Media Kedokteran Hewan Universa Medicina journal of Basic Medical Veterinary Indonesian Andrology and Biomedical Journal
Rahmi Sugihartuti, Rahmi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Increase in neutrophil count after repeated exposure of Plasmodium berghei-infected mice to artemisinin Maslachah, Lilik; Sugihartuti, Rahmi
Universa Medicina Vol 36, No 1 (2017)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2017.v36.49-58

Abstract

Background Leukocytes play an important role in the elimination of malaria infection. The leukocyte profile upon elimination of the malaria parasites that have been exposed to antimalarials and are subsequently capable of faster growth has not been researched. The aim of this research was to evaluate the role of mouse leukocytes in the elimination of parasites as shown by the leukocyte profile.Methods An experimental research with post test only control group design was conducted involving 24 male mice of the Swiss Albino strain weighing 20 g -30 g, and 2.5 months old. They were randomized into four groups: two control groups (K1, KP) and two treatment groups (P1, P4). Artemisinin at a dose of 0.04 mg/g body weight was given to the mice for 3 days, starting 2 days after infection. The leukocyte profile was observed on the 2nd, 5th, 8th, and 10th day after infection. The results were analyzed by two-way Anova.Results As shown in treatment control group KP and treatment group P4, P. berghei that had been passaged in the mice and were still viable after repeated exposure to artemisinin, may cause changes in leukocyte profile. On the 10th day of infection, the neutrophil percentage in group P1 showed a significantly different decrease when compared with the other groups (K1, KP and P4) (p<0.05).Conclusion Repeated exposure to artemisinin of mice infected with P. berghei can cause changes in neutrophil profile in mice.
EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Ayu, Zerlinda Dyah; Maslachah, Lilik; Supranianondo, Koesnoto; Suprihati, Endang; Plumeriastuti, Hani; Sugihartuti, Rahmi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091

Abstract

The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Ameliorative Effects of Eleutherine palmifolia Extract on Monosodium Glutamate-Induced Reproductive Toxicity: Modulation of Oxidative Stress Markers and Spermatogenesis in a Murine Model Rahmatullah, Aldin Akbar; Ratnaningtyas, Nurrohmah; Mustofa, Imam; Mulyati, Sri; Sugihartuti, Rahmi; susilowati, Suherni; Putri, Cindy Ercha Aulia
Indonesian Andrology and Biomedical Journal Vol. 6 No. 1 (2025): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v6i1.72293

Abstract

Background : Chronic monosodium glutamate (MSG) exposure is linked to testicular toxicity, impacting spermatogenesis and oxidative stress markers. Objective : This study investigated the protective effects of Dayak onion (Eleutherine palmifolia) extract against MSG-induced testicular damage in mice. Methods : Twenty-five male mice (11 weeks, ~20 g) were divided into five groups: negative control (C-, 0.5% CMC-Na), positive control (C+, MSG 4 mg/g BW), and treatment groups (T1, T2, T3) receiving MSG plus Dayak onion extract (30, 60, 120 mg/kg BW, respectively) for 52 days. On day 53, testicular histology and serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were analyzed. One-way ANOVA and Duncan’s post hoc test assessed differences (p<0.05). Results : Group C+ showed significantly reduced spermatogenic cell counts, elevated MDA, and decreased SOD compared to C-. Group T1 exhibited no significant improvement in spermatogonia and spermatocytes but increased spermatids. Groups T2 and T3 significantly improved spermatogenic cell counts, with T3 showing the closest recovery to C-. MDA levels decreased, and SOD activity increased dose-dependently, with T3 nearing C- levels. Conclusion : Dayak onion extract, particularly at 120 mg/kg BW, mitigates MSG-induced testicular toxicity, preserving spermatogenesis and balancing oxidative stress markers.
Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate Rahmatullah, Aldin Akbar; Sugihartuti, Rahmi; Susilowati, Suherni; Hamid, Iwan Sahrial; Suprayogi, Tri Wahyu; Rachmawati, Kadek; Safitri, Erma; Mustofa, Imam; Mulyati, Sri
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.113-122

Abstract

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the motility, viability, and concentration of spermatozoa in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (CMC-Na 0.5%), C+ (4 mg/g BW of MSG), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg BW of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The results showed no significant differences in the motility and concentration of spermatozoa between the C- (81.50 ± 4.18; 7.21 ± 0.37) and T3 (77 ± 2.74; 7.04 ± 0.13) groups (p < 0.05). In addition, the results showed significant differences in the viability of spermatozoa between the C- (83.30 ± 3.40) and T3 (77.20 ± 4.06) groups. Therefore, it can be concluded that the administration of Dayak onion extracts orally to mice induced with MSG can maintain the motility, viability, and concentration of spermatozoa.
Protective Effect of The Extract of Dayak Onions (Eleutherine palmifolia) on Sertoli and Leydig Cell Necrosis in Mice (Mus Musculus) Induced with Monosodium Glutamate Rahmatullah, Aldin Akbar; Ratnaningtyas, Nurrohmah; Sugihartuti, Rahmi; Susilowati, Suherni; Safitri, Erma; Mulyati, Sri
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.87-96

Abstract

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the number of necrotic Sertoli and Leydig cells in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (0.5% CMC-Na), C+ (4 mg/g BW of MSG and 0.5% CMC-Na), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The mice were euthanized on day 53 of the experiment. Their testicles were removed and used to prepare histological specimens with the H&E staining method. The results showed significant differences (p < 0.05) in the number of necrotic Sertoli and Leydig cells between the C+ group and the T1, T2, and T3 groups with gradually decreasing values. The results suggested that the administration of the extract of Dayak onions can prevent Sertoli and Leydig cell necrosis in mice induced with MSG at an optimal dose of 120 mg/kg BW.
Co-Authors Adiana Mutamsari Witaningrum Ayu, Zerlinda Dyah Chusniati, Sri Dadik Rahardjo, Dadik Endang Suprihati Erma Safitri Hani Plumeriastuti Hisyam, Mirza Atikah Madarina IMAM MUSTOFA Iwan Sahrial Hamid Kadek Rachmawati Lilik Maslachah Munawer Pradana Putri, Cindy Ercha Aulia Raharjo, Hartanto Mulyo Rahmatullah, Aldin Akbar Ratnaningtyas, Nurrohmah Saraswati, Afif Tasya Soeharsono Soeharsono Sri Mulyati Suherni Susilowati Supranianondo, Koesnoto Tacharina, Martia Rani Tri Wahyu Suprayogi Tyaningsih, Wiwiek Yulianna Puspitasari