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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences. Journal of Fisheries & Marine Jurnal Agro Veteriner

Lilik Maslachah

Department Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia

Author-ID : 4007419

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Increase in neutrophil count after repeated exposure of Plasmodium berghei-infected mice to artemisinin Maslachah, Lilik; Sugihartuti, Rahmi
Universa Medicina Vol 36, No 1 (2017)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2017.v36.49-58

Background Leukocytes play an important role in the elimination of malaria infection. The leukocyte profile upon elimination of the malaria parasites that have been exposed to antimalarials and are subsequently capable of faster growth has not been researched. The aim of this research was to evaluate the role of mouse leukocytes in the elimination of parasites as shown by the leukocyte profile.Methods An experimental research with post test only control group design was conducted involving 24 male mice of the Swiss Albino strain weighing 20 g -30 g, and 2.5 months old. They were randomized into four groups: two control groups (K1, KP) and two treatment groups (P1, P4). Artemisinin at a dose of 0.04 mg/g body weight was given to the mice for 3 days, starting 2 days after infection. The leukocyte profile was observed on the 2nd, 5th, 8th, and 10th day after infection. The results were analyzed by two-way Anova.Results As shown in treatment control group KP and treatment group P4, P. berghei that had been passaged in the mice and were still viable after repeated exposure to artemisinin, may cause changes in leukocyte profile. On the 10th day of infection, the neutrophil percentage in group P1 showed a significantly different decrease when compared with the other groups (K1, KP and P4) (p<0.05).Conclusion Repeated exposure to artemisinin of mice infected with P. berghei can cause changes in neutrophil profile in mice.

Perubahan Profil Proteomik Plasmodium falciparum Galur Papua 2300 Akibat Paparan Antimalaria Artemisinin In Vitro Maslachah, Lilik
Jurnal Kedokteran Brawijaya Vol 29, No 1 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2016.029.01.10

Perkembangan resistensi Plasmodium falciparum dan penurunan kepekaan parasit terhadap obat antimalaria artemisinin menjadi salah satu permasalahan kesehatan di dunia. Sampai saat ini belum ada obat baru pengganti artemisinin. Penelitian ini bertujuan untuk membuktikan bahwa paparan obat antimalaria artemisinin berulang in vitro dapat menyebabkan perubahan profil protein melalui pendekatan proteomik P. falciparum galur Papua 2300. Waktu penelitian dilaksanakan mulai bulan Pebruari sampai dengan Nopember 2014. Tempat penelitian di Laboratorium Biomedik Fakultas Kedokteran Universitas Brawijaya, Fakultas Kedokteran Hewan Universitas Airlangga. Airlangga Influenza Research Center, Laboratorium bersama Kimia Â Universitas Negeri Surabaya (UNESA) dan jurusan Kimia Politeknik Malang. Desain penelitian yang digunakan adalah Experimental Design dengan Post test only control group design. Kultur P. falciparum galur Papua 2300 dipapar antimalaria artemisinin berulang dengan menggunakan Inhibitory Concentration 50 (IC50). Pengamatan dilakukan terhadap profil protein dengan SDS Page 2 dimensi, FT- IR dan LCMS. Hasil penelitian menunjukkan ada variasi berat protein, spektrum infra merah (bilangan gelombang) Â dan nilai massa molekul ion (m/z) Â antara kelompok kontrol (K) dan kelompok perlakuan (PO1, PO2, Po3, PO4). Dapat disimpulkan bahwa paparan obat antimalaria artemisinin berulang secara Â in vitro dapat mempengaruhi pola ekspresi protein Plasmodium falciparum galur Papua 2300.Â

PENGARUH PAPARAN ARTEMISININ TERHADAP EKSPRESI GEN PART PADA PLASMODIUM FALCIPARUM GALUR PAPUA 2300 Plumeriastuti, Hani; Maslachah, Lilik; Nidom, Chairul A.
Majalah Kedokteran Bandung Vol 47, No 3 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.763 KB)

Plasmodium resisten terhadap artemisinin menjadi salah satu permasalahan kesehatan di dunia karena belum ada obat baru pengganti artemisinin. Resistensi P. falciparum terhadap obat antimalaria artemisinin dapat terjadi karena dipengaruhi oleh faktor internal dari P. falciparum, antara lain induksi ekspresi gen yang mengekspresikan protein. Salah satu gen tersebut adalah gen Triptophan-rich Protein (PArt). Fungsi Triptophan-rich Protein penting dalam membrane-spanning protein dan berperan dalam folding protein untuk menjaga kontak hidrofobik. Penelitian ini bertujuan membuktikan overekspresi gen Triptophan-rich Protein P. falciparum galur Papua 2300 yang disebabkan oleh paparan artemisinin berulang in vitro. Waktu penelitian dilaksanakan bulan Februari sampai November 2013. Tempat penelitian di Rumah Sakit Penyakit Tropik dan Infeksi Universitas Airlangga. Desain penelitian yang digunakan adalah experimental design dengan post test only control group design. Kultur in vitro P. falciparum galur Papua 2300 dibagi dalam kelompok kontrol (K) dan kelompok perlakuan paparan artemisinin berulang, yaitu paparan artemisinin ke-1 (PO1), paparan artemisinin ke-2 (PO2) dan paparan artemisinin ke-3 (PO3) menggunakan konsentrasi IC50. Ekspresi gen Triptophan-rich Protein (PArt) diukur dengan qRTPCR. Hasil menunjukkan paparan artemisinin berulang pada P. falciparum dapat meningkatkan level ekspresi gen Part (2??CT) relatif terhadap kontrol. Simpulan, paparan artemisinin in vitro menyebabkan overekspresi gen Tryptophan-rich Proteins(PArt) oleh promoter P. falciparum galur Papua 2300. [MKB. 2015;47(3):129?36]Kata kunci: Artemisinin, fenotip, gen Triptophan-rich Protein (PArt), P. falciparum galur Papua 2300 Effect of Artemisinin Exposure toward PArt Gene Expression in Plasmodium falciparum Papua 2300 StrainAbstract Artemisinin resistant Plasmodium has become one of the worldwide health problems, since there is currently no new therapeutic medicine to replace artemisinin. Even though the mechanism of artemisinin resistance has not been clearly understood, the resistance of P. falciparum towards the antimalaria artemisinin may occur due to the influence of by the internal factors of P. falciparum, including the induction of the protein-expressing gene expression. One of the genes is the Triptophan-rich Protein (PArt) gene that is important in the membrane-spanning protein and plays a role in protein folding to maintain hydrophobic contact.. This study aimed to prove that Triptophan-rich Protein overexspression in P. falciparum Papua 2300 strain may cause repeated artemisin exposure in vitro. This study was performed in a period from February to November 2013 in Infection and Tropical Diseases Hospital, Airlangga University. The design used was experimental study with post-test only control group design. In-vitro culture of P. falciparum Papua 2300 strain were divided into a control group (K) and treatment groups that were treated regularly with artemisinin, i.e. artemisinin exposure I (PO1), artemisinin exposure 2 (PO2) and artemisinin exposure 3 (PO3) using IC50 concentration. The Tryptophan-rich Protein gene expression level was detected using qRTPCR. The result showed that in vitro repeated artemisinin exposure in P. falciparum Papua 2300 strain relatively increased the expression level of the Tryptophan-rich Protein (PArt) genes (2??CT) when comparedwith control. In conclusion, in vitro artemisinin exposure may cause Tryptophan-rich Proteins (PArt) gene overexpression by P. falciparum Papua 2300 strain promoter. [MKB. 2015;47(3):129?36]Key words: Artemisinin, phenotype, Triptophan-rich Protein (PArt) gene, P. falciparum Papua 2300 DOI: 10.15395/mkb.v47n3.593

Profil Fenotipik Plasmodium falciparum Galur Papua 2300 Akibat Paparan Antimalaria Artemisinin in Vitro Maslachah, Lilik; Dachlan, Yoes Prijatna; Nidom, Chairul A.; Fitri, Loeki Enggar
Majalah Kedokteran Bandung Vol 47, No 1 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.484 KB)

Resistensi parasit P. falciparum dan penurunan efikasi terhadap artemisinin mengakibatkan masalah malaria menjadi semakinÂ kompleks. Hal ini menjadi salah satu permasalahan kesehatan di duniaÂ yang belum dapat diselesaikan sampai saat ini karena belum ada obat baru pengganti artemisinin. Penelitian ini untuk membuktikan bahwa paparan obat antimalaria artemisinin berulang in vitro dapat menyebabkan perubahan profil fenotipik P. falciparum galur Papua 2300. Waktu penelitian Februari sampai dengan November 2013. Tempat penelitian di Biomedik Universitas Brawijaya Malang dan Fakultas Kedokteran Hewan Universitas Airlangga. Desain penelitian experimental design dengan post test only control group design. Kultur P. falciparum galur Papua 2300 dipapar artemisinin berulang dengan dosis IC50. Pengamatan dilakukan terhadap viabilitas dan nilai IC50 dengan menggunakan analisis probit. Kelompok kontrol tidak menunjukkan perubahan nilai IC50 juga pada kelompok perlakuan PO1. Nilai IC50 terjadi peningkatan setelah perlakuan PO2. Paparan artemisinin berulang pada PO2, PO3, dan PO4 menyebabkan waktu viabilitas P. falciparum galur Papua 2300 lebih pendek daripada PO1. Viabilitas stabil setelah perlakuan PO3. Simpulan, paparan artemisinin berulang berpengaruh pada perubahan peningkatkan nilai IC50 dan waktu viabilitas P. falciparum galur Papua 2300.Â [MKB. 2015;47(1):1â€“9]Kata kunci: Artemisinin, fenotipik, P. falciparum galur Papua 2300, resistensiPhenotypic Profile ofÂ Plasmodium falciparum Papua 2300 Strain Exposed to in Vitro Antimalarial Artemisinin The presence of the P. falciparum resistance and decreased of efficacy against artemisinin and its derivatives result in increasingly complex malaria issues. Malaria has become one of the currently unresolved worldâ€™s health problems due to the lack ofÂ new artemisinin replacement drugs. This study aimed to provide evidence that the repeated exposure of in vitro artemisinin may cause a change in P. falciparum Papua 2300 strain phenotypic. This study was conducted during the period ofÂ February to November 2013 in Biomedics Brawijaya University and the Faculty of Veterinary Medicine, Airlangga University. A post-test control only experimental design was used. In vitro cultures ofÂ P. falciparum Papua 2300 strain were treated by repeated artemisin in IC50 concentration and were observed for their viability and IC50 using probit analysis. The control group did not show any changes after IC50value and PO1 treatment. An increase in IC50 value was occurred after PO2. Repeated exposures of artemisinin in PO2, PO3 and PO4 had shorter viability periods than PO1. The viability of was stable after PO3 in this group. In conclusion, repeated exposures of artemisinin influence changes inÂ IC50 value and viability period ofÂ P. falciparum Papua 2300 strain. [MKB. 2015;47(1):1â€“9]Key words: Artemisinin, phenotypic, P. falciparum Papua 2300, resistanceÂ DOI: 10.15395/mkb.v47n1.390Â Â Â

Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing Lilik Maslachah; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product

Induction of Plasmodium falciparum strain 2300 dormant forms by artemisinin Lilik Maslachah; Yoes Prijatna Dachlan; Chairul A. Nidom; Loeki Enggar Fitri
Universa Medicina Vol. 34 No. 1 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.25-34

BACKGROUND The presence of Plasmodium falciparum resistance and decreased efficacy of artemisinin and its derivatives has resulted in the issue of malaria becoming increasingly complex, because there have been no new drugs as artemisinin replacements. The aims of this research were to evaluate in vitro changes in ultrastructural morphology of P. falciparum 2300 strain after exposure to artemisinin. METHODS The research used an experimental design with post test only control group. Cultures of P. falciparum 2300 strain in one control and one mutant group were treated by exposure to artemisinin at IC50 10-7 M for 48 hours. Ultrastructural phenotypic examination of ring, trophozoite and schizont morphology and developmental stage in the control and mutant group were done at 0, 12, 24, 36, 48 hours by making thin blood smears stained with 20% Giemsa for 20 minutes and examined using a microscope light at 1000x magnification. RESULTS Dormant forms occurred after 48 hours of incubation with IC50 10-7 M artemisinin in the control group. In the mutant group, dormant forms, trophozoites with blue cytoplasm and normal schizont developmental stages were seen. Ultrastructural phenotypic morphology at 0, 12, 24, 36, 48 hours showed that in the control group dormant formation already occurred with exposure to IC50 10-7 M, while in the mutant group dormant formation occurred only with exposure to IC50 2.5x10-5 M. CONCLUSION Exposure to artemisinin antimalarials in vitro can cause phenotypic morphological changes of dormancy in P. falciparum Papua 2300 strain.

Repellent Effectiveness of Permot Leaf Ethanol Extract (Passiflora Foetida Linn.) against Aedes Aegypti Adult Mosquitoes David Mohamad Qadafi; Poedji Hastutiek; Lilik Maslachah; Endang Suprihati; Muhammad Hambal
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

Indonesia is one of the largest tropical countries in the world and various diseases can arise in the tropics which are caused by animals as vectors. An example of a vector that can carry diseases is a mosquito. Mosquitoes are insects that live side by side with humans buy act as vectors of disease. Mosquito Aedes aegypti is a type of mosquito that can carry the virus that causes Dengue Hemorrhagic Fever. This research was conducted to determine the effectiveness of Permot leaf ethanol extract (Passiflora foetida L.) as an alternative repellent against adult mosquitoes Aedes aegypti. This research was conducted from October to December 2020 and used Permot leaf ethanol extract consisting of 3 cream concentrations, namely 2.5%, 5%, 7.5%, negative control using cream without permot leaf ethanol extract and positive control using mosquito cream. The data of this study were tested using one way ANOVA to find the effectiveness rate and comparations of the each Permot leaf repellent extract. This study proven that the permot leaf ethanol extract is effective as a repellent for Aedes aegypti mosquitoes.

The Activity of Mixed Microalgae Polysaccharides from Indonesia as Anti-Malaria in Vitro Mahendra Pujiyanto; Zhaza Afililla; Lilik Maslachah; Thomas Valentinus Widiyatno; Mochamad Donny Koerniawan; Eko Agus Suyono; Arief Budiman; Ulfah Juniarti Siregar; Lucia Tri Suwanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.34766

Highlight Research One of the content of microalgae that is beneficial for health is polysaccharides Polysaccharides of Indonesian microalgae can be promoted as anti-malarial Polysaccharides from Glagah, Spirulina and East Java microalgae inhibited the growth of plasmodium in vitro and had IC50 values of 3.18 µg/mL, 5.43µg/mL and 9.87 µg/mL, respectively Abstract Malaria is an infectious disease caused by protozoan parasites of the genus Plasmodium that categorized as deadliest diseases in the world. Artemisinin and its derivatives are still recommended drugs for malaria therapy, however, there have been indications that Plasmodium parasites are resistant to this drug. Therefore, a study on polysaccharides from microalgae may be a potential as bioactive compound for anti-malaria. The aim of this study was to determine the effectiveness of the mixed microalgae polysaccharides as anti-malarial in vitro. Polysaccharides were extracted from three microalgae Spirulina sp., mixed microalgae Glagah and mixed microalgae East Java using the alkaline extraction method. The anti-malarial activity test refers to the concentration of polysaccharides used in calculating the IC50 value by probit analysis. The concentration of polysaccharides of the three microalgae used were 0; 0.01; 0.01, 1, 10 and 100 µg/mL. The results showed that the IC50 values of polysaccharides of Glagah, Spirulina sp. and East Java microalgae were 3.18 µg/mL, 5.43µg/mL, and 9.87 µg/mL, respectively. In Conclusion, polysaccharides of Indonesian mixed microalgae can be promoted as anti-malarial.

Effect of Combination of Probiotics and Moringa oleifera Leaf Extract on Nutrients Intake in Ducks Lokapirnasari, Widya Paramita; Al Arif, Mohammad Anam; Maslachah, Lilik; Chandra, Evania Haris; Utomo, Gogik Satrio Margo; Yulianto, Andreas Berny
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.241-246

The purpose of this research was to determine the effect of combination probiotics and Moringa oleifera (M. oleifera) extract in nutrient consumption of ducks. This study used 48 Peking ducks. The concentration of probiotic was 1.2 x 108 CFU/ml. The treatments of this research were P0 (control), P1 (4 ml probiotics), P2 (4 ml M. oleifera extract) and P3 (2 ml probiotics + 2 ml M. oleifera extract). The data were statistically analyzed by Analysis of Variance (ANOVA). The results showed that feed consumption of dry matter, organic matter, crude protein, crude fat, crude fiber and nitrogen free extract were no significant difference (p>0.05) between all treatments. It can be concluded that the addition of combination probiotics and M. oleifera extract can be used to maintain nutrient consumption and safe for health ducks.

Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats Al-Anshori, Akhmad Afifudin; Putri, Anandia Nafisah; Ismi, Alif Noviana; Suhud, Mohamad Kharis; Plumeriastuti, Hani; Maslachah, Lilik
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.145-154

This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin.

Co-Authors Adiana Mutamsari Witaningrum Agustina, Firanda Al Arif, M Anam Al arif, Mohammad Anam Al-Anshori, Akhmad Afifudin Andreas Berny Yulianto, Andreas Berny Arief Budiman Ayu, Zerlinda Dyah Boedi Setiawan Chairul A. Nidom Chairul A. Nidom Chairul Anwar Chandra, Evania Haris Cholifah, Siti Chusnul Chusniati, Sri Dadik Rahardjo, Dadik David Mohamad Qadafi Dewi, Aprilia Kurnia Eko Agus Suyono Endang Suprihati Hani Plumeriastuti Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indriyanti, Astrid Ismi, Alif Noviana Kadek Rachmawati Khoirunnisa, Itrah Koestanti S, Emy Legowo, Djoko Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mahendra Pujiyanto Mirino, Mario Navyseal Mochamad Donny Koerniawan Mochamad Lazuardi Muhammad Hambal Munawer Pradana Mustofa Helmi Effendi Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poedji Hastutiek Pratama, Abrian Panggalih Indra Pujiyanto, Mahendra Putri, Anandia Nafisah Raharjo, Hartanto Mulyo Rahmi Sugihartuti, Rahmi Ratna Damayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Saputra, Rakan Mahiid Saraswati, Afif Tasya Sarudji, Suryanie Soeharsono Soeharsono Sri Mulyati Suherni Susilowati Suhud, Mohamad Kharis Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Supranianondo, Koesnoto Suprihati , Endang Supriyadi Suzanita Utama Tacharina, Martia Rani Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tri Wahyu Suprayogi Tyaningsih, Wiwiek Ulfah Juniarti Siregar Utomo, Gogik Satrio Margo Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Yoes Prijatna Dachlan Yulianna Puspitasari Zhaza Afililla

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