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All Journal Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Perairan (Aquatic Science) JOIV : International Journal on Informatics Visualization Unri Conference Series: Community Engagement Journal of Coastal and Ocean Sciences Journal of Rural and Urban Community Empowerment Journal of Community Engagement Research for Sustainability Jurnal Abdimas: Pengabdian dan Pengembangan Masyarakat Jurnal Perikanan dan Kelautan
Mardalisa, Mardalisa
Universitas Riau
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Nursing Physics Public Health Social Sciences Other

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Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique - Mardalisa; Sony Suhandono; Novi Yanti; Fazrol Rozi; Fitri Nova; - Primawati
JOIV : International Journal on Informatics Visualization Vol 5, No 2 (2021)
Publisher : Society of Visual Informatics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30630/joiv.5.2.459

Abstract

Bioinformatics has developed into an application tool for basic and applied research in the biomedical and biotechnology field. Polymerase Chain Reaction (PCR) is a common technique in the molecular area that has always involved bioinformatics science. PCR cloning techniques such as TA cloning and PCR-mediated cloning exhibit complex processes with low success rates. One easy, effective, and practical solution is to use a mega-primer with the Overlap Extension PCR Cloning (OEPC) technique. The success of PCR cloning using the mega-primer design in the OEPC technique is strongly influenced by the characteristics of the mega-primer used. Knowledge of mega-primer characteristics is one of the important factors in the success of PCR cloning. The design process for the mega-primer str promoter was characterized based on the principle of a genetic algorithm using the web-based bioinformatics tools such as ClustalW, NetPrimer, and BLAST. The success of the mega-primer construction in producing recombinant pSB1C3 vector has been confirmed by the sequencing method and the function of the reporting protein (AmilCP). DNA analysis shows a 100 % homologous sequence on the str promoter, while  E. coli colonies successfully express the purplish-blue color. Mega-primer characters can save costs and time of the research by maintaining the primer parameters that provide optimal values and increase the success value of PCR cloning via bioinformatics software. Hence, implications on biological problems, especially using DNA and amino acid sequences, could solve rapidly.
Isolation and Identification of Indigenous Plastic-Degrading Bacteria from Dumai’s Ocean Water of Riau Province Mardalisa Mardalisa; Eza Buana Fatwa; Dessy Yoswaty; Feliatra Feliatra; Irwan Effendi; Bintal Amin
Ilmu Perairan (Aquatic Science) Vol 9, No 1 (2021)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.9.1.p.77-85

Abstract

One of the interesting and environmentally friendly microbiology strategies and approaches to control the impact of microplastics is to approach bioremediation technology by harnessing the potential of microbes or indigenous bacteria (local bacteria).  Dumai sea waters currently show a high enough of microplastic pollution which allows the potential of indigenous bacteria to adapt to a plastic environment. The purpose of this study is to isolate and identify indigenous bacteria to degrade plastics from the sea waters of Dumai and to know whether or not there is a difference in the number of bacteria found between stations in this study. This research was conducted in October-December 2020 with experimental methods at the Marine Microbiology Laboratory Faculty of Fisheries and Marine Science Universitas Riau. Based on the results of the study, 12 bacterial isolates were isolated from the research stations.  Isolates of these bacteria have diamaters ranging from 0.2-1.1 cm. Microplastic degradation test results by bacteria found that ISL 10 is an isolate that shows the highest PET degradation activity, which is 17.27% and the diameter of biofilm formation 0.8 cm. Based on biochemical and morphological tests, similar results were obtained that ISL 10 bacteria are a bacterium of the genus Bacillus. The most bacterial colonies were seen in statiun IV (TPI) with an average number of bacteria of 214.9 x 104 CFU/ml.
Multiple Antibiotic Resistance Index of Escherichia coli Isolates from Dumai Sea Waters Riau Province Mardalisa Mardalisa; Feliatra Feliatra; Nursyirwani Nursyirwani
Berkala Perikanan Terubuk Vol 49, No 1 (2021): Februari 2021
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/terubuk.49.1.734-739

Abstract

The pattern of antibiotic consumption by the community which is carried out independently without medical guidance causes antibiotic pollution has reached sea waters, one of its impacts on the nature of bacterial resistance. This study aims to look at the pattern of antibiotic sensitivity to E coli that isolated from Dumai sea waters. E coli isolates that grown on MHA media were tested based on the Kirby Bauer disk diffusion method. Seventeen isolates were identified, among which a high rate of resistance to penicillin, isoniazid and streptomycin (17), a high rate of resistance to chloramphenicol (5), intermediate susceptibility to chloramphenicol (11) and only 1 sensitive to chloramphenicol. The multiple antibiotic resistance (MAR) index of E coli isolates was 0.82. These show that antibiotics like penicillin, isoniazid, streptomycin and chloramphenicol were not suitable antibiotic for E coli infection treatment. The community is expected to be more disciplined in using antibiotics and to be careful in using the Dumai sea for fishery activities
PERBERDAYAAN IBU RUMAH TANGGA MELALUI BAKSO DAN NUGET IKAN BERSAMA LAZISMU PEKANBARU Irwan Effendi; Mardalisa; Ummi Mardhiah Batubara; Rita Wijaya; Muhammad Arief Wibowo
Journal of Rural and Urban Community Empowerment Vol. 2 No. 1 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.935 KB) | DOI: 10.31258/jruce.2.1.63-68

Abstract

Poverty is not only caused by the low income of the husbands, but also because family members are not optimally empowered. LAZISMU is a national level zakat institution that is active in community empowerment. One of the targets of its activities is social and economic activities in the form of forming and coaching as well as funding community business groups. This community service program involved housewives and several young women in Kota Pekanbaru. This service is in the form of assistance to the participants so that they have knowledge about processing and varying the methods of processing fish commodities to become fish balls and nuggets. The participants were generally very interested in the training material for making meatballs and fish nuggets. All groups successfully finished making meatballs and fish nuggets with quite savory flavors.
Peningkatan Pendapatan Nelayan Di Desa Teluk Rhu Kabupaten Bengkalis Melalui Teknologi Diversifikasi Pangan Berbahan Dasar Hasil Perikanan Feliatra Feliatra; Ummi Mardhiah Batubara; Mardalisa Mardalisa; Pipin Kurnia; Iesje Lukistyowati; Tengku Muhammad Ghazali
Journal of Community Engagement Research for Sustainability Vol. 2 No. 2 (2022): March
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.668 KB) | DOI: 10.31258/cers.2.2.70-77

Abstract

Teluk Rhu Village is a village that has potential in terms of fisheries and marine tourism. Some of the people in this village are fishermen, and the rest are fish farmers. The minimum knowledge and competence have fishermen in Teluk Rhu Village cause the welfare to be relatively low. To improve the fishermen's income needed knowledge improvement through food diversification technology based on fishery products. There are four methods used in this program, such as observation, material delivery, product manufacturing practices, and program evaluation. Twenty fishermen were participants and were involved very enthusiastically and actively in this program. There were two materials presented to increase fishermen's knowledge, including digital literacy and food innovation products. Lastly, all participants were trained to make several processed food products such as fish balls, nuggets, and fish sticks. The questionnaire data show that the community service program had several benefits for most participants. Thus, it's hoped that the new knowledge can make them more enthusiastic and motivated to begin a new businesses to increase family income.
Characteristics of Salted Catfish (Pangasius hypophthalmus) with the Addition of Green Tea and Lemongrass Mardalisa Mardalisa; Alqoriffah Hasanah; Mutia Dhiya Amani; Bella Permata Azzahara
Jurnal Perikanan dan Kelautan Vol 27, No 2 (2022): June 2022
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.256-263

Abstract

This study aims to determine the characteristics of salted catfish after the addition of green tea and lemongrass. This research method used a randomized block design (RAK) with one treatment factor and was carried out with 3 repetitions. The treatments used were the addition of 1% green tea and 0,15% lemongrass. The results showed that the treatment had no significant effect on the yield value, water content, ash content, lipid content and protein content of the salted catfish produced. The results of sensory analysis showed that the treatment had a significant effect on appearance, while for aroma, taste and texture it had no significant effect. The best product is with the addition of 0,15% lemongrass which produces a yield value of 57,25%, water content 2.21%, ash content 24,42%, lipid content 29,53%, and protein content 42,96%. Sensory analysis scores for appearance were 7,33 (whole, clean, lusterless), aroma 6,54 (neutral, little additional odor), taste 6,62 (good, species specific, little taste addition), and texture 6,93 (dense, no brittle).
Peningkatan Penghasilan Rumah Tangga Nelayan Melalui Pengolahan Hasil Tangkap Nelayan Desa Teluk Rhu Kec. Rupat Utara Kab. Bengkalis Feli Feliatra; Mardalisa Mardalisa; Ummi Mardhiah Batubara; Iesje Lukystiowati; Pipin Kurnia; Tengku Muhammad Ghazali
Jurnal Abdimas: Pengabdian dan Pengembangan Masyarakat Vol 3 No 1 (2021)
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.896 KB)

Abstract

Kegiatan pengabdian ini merupakan salah satu wahana untuk meningkatkan perekonomian rakyat yang dilakukan oleh istri nelayan. Pelatihan diberikan kepada dua kelompok usaha nelayan Desa Teluk Rhu dalam mengolah ikan menjadi produk yang memiliki nilai jual tinggi berupa nugget, bakso, kerupuk dan stik ikan. Kegiatan pengabdian ini meliputi tiga tahapan inti berupa ceramah terkait literasi digital dan inovasi produk olahan ikan, diskusi dan pengisian kuisioner, serta praktek pembuatan produk diversifikasi. Hasil observasi menunjukkan bahwa kelompok usaha nelayan Desa Teluk Rhu memiliki potensi dalam memproduksi bahan makanan berbahan dasar ikan dikarenakan tingginya ketersediaan bahan baku. Hasil pelatihan dari 20 peserta menyatakan bahwa kegiatan ini sangat bermanfaat dan berkeinginan untuk mengaplikasikan ilmu yang mereka dapatnya. Produk yang paling disukai adalah bakso dan nugget ikan dikarenakan prosesnya yang mudah dan rasanya yang enak. Kedepannya masyarakat diharapkan mampu untuk memulai usaha produk olahan ikan untuk meningkatkan pendapatan rumah tangga
Pengembangan wisata pantai melalui peran serta kelompok IRT Nelayan Di Desa Teluk Rhu Rupat Utara Feliatra Feliatra; Ummi Mardhiah Batubara; Iesje Lukistyowati; Mardalisa Mardalisa; Lamun Batara
Unri Conference Series: Community Engagement Vol 4 (2022): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.4.109-116

Abstract

One of the potentials in Teluk Rhu Village is beach tourism. The development of coastal tourism in Teluk Rhu Village needed a principal role from the Women's Fishermen, specifically to make processed products from fishermen's catch. The community service activity aims to improve the Women Fishermen's knowledge and skills in making processed fishery products that can be marketed at coastal tourist locations. This program implementation used the PALS (Participatory Action and Learning System) method with three stages. There was socialization, increasing knowledge, and direct practice. The community service activities showed that there was potential to develop coastal tourism in Teluk Rhu Village. The several potentials were the Women Fishermen can get the raw material easily, they have a lot of time used to produce and sell the products, and they have skills to make some processed food with diversification techniques such as nugget, meatball, and otak-otak. Thus, Increasing the knowledge and skills of the Women's fishermen through diversification technology can be used to increase the number of tourists at the coastal tourist location of Teluk Rhu Village, Rupat Utara District.
Characteristics of Salted Catfish (Pangasius hypophthalmus) with the Addition of Green Tea and Lemongrass Mardalisa Mardalisa; Alqoriffah Hasanah; Mutia Dhiya Amani; Bella Permata
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.256-263

Abstract

This study aims to characteristics the salted catfish after the combination of adding green tea and lemongrass. The research method used a randomized block design (RAK) which involved one treatment factor with 3 repetitions so that there were 12 units. The factors used in the treatment were 3% salt solution, 1% green tea and 0,15% lemongrass. This study stated that there was no significant effect of the four treatment levels on the results of the assessment of yield, ash content, water content, lipid content and protein content in salted catfish produced. Sensory analysis on the appearance, taste, aroma, and texture aspects showed that the treatment result had a significant effect only on the appearance aspect. The best product was found in the X2 treatment (3% salt solution dan 0.15% lemongrass) which resulted in a yield value of 57,25%, ash content 24,42%, water content 2.21%, lipid content 29,53%, and protein content 42,96%. Sensory analysis scores for appearance were 7,33 (whole, clean, lusterless), aroma 6,54 (neutral, little additional odor), taste 6,62 (good, species specific, little taste addition), and texture 6,93 (dense, no brittle).
Analysis of the Effectiveness of Mango Leaves (Mangifera indica) as a Phytoimunostimulant on Common Carp (Cyprinus carpio) in Brackish Water Saputra, Andika; Effendi, Irwan; Mardalisa, Mardalisa
Journal of Coastal and Ocean Sciences Vol. 4 No. 1 (2023): January
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The problems that are often experienced when cultivating goldfish are diseases caused by pathogens that attack cultivated fish. The cause of disease in fish is caused by interactions between fish, pathogens and their habitat environment that can cause death in fish. The solution to this problem is to provide a phytoimmunostimulant derived from mango leaves (Mangifera indica). The purpose of this study was to determine the effect of mango leaves on the hematology and physiology of common carp. This research was conducted in January-April 2022. The method used in this research is an experimental method by comparing data. Parameters observed were total erythrocytes, hemoglobin, hematocrit, blood glucose, absolute body weight growth, survival rate, and water quality. Based on the research that has been done, it can be concluded that the addition of 1.5% mango leaf powder into fish feed as a phytoimmunostimulant affects the hematological and physiological effects of common carp. Common carp treated with phytoimmunostimulants in fresh water had higher mean values ​​(total erythrocytes 1.75x106 cells/mm3, hematocrit 33.66%, and hemoglobin 8.13 g/dL) compared to data in brackish water (total erythrocytes 1.54x106 cells/mm3, hematocrit 30.66%, and hemoglobin 7.76 g/dL).
Co-Authors - Primawati Alqoriffah Hasanah Amani, Mutia Dhiya ANDIKA SAPUTRA Batara, Lamun Batubara, Ummi Mardhiah Bella Permata Bella Permata Azzahara Bintal Amin Dessy Yoswaty Dessy Yoswaty Eza Buana Fatwa Eza Buana Fatwa Fatwa, Eza Buana Fazrol Rozi, Fazrol Feliatra Feliatra, Feli Fitri Nova Hasanah, Alqoriffah Iesje Lukistyowati Iesje Lukystiowati Irwan Effendi Irwan Effendi Irwan Irwan Laksono Trisnantoro Lamun Batara Muhammad Arief Wibowo Mutia Dhiya Amani Nursyirwani, Nursyirwani Permata, Bella Pipin Kurnia Pipin Kurnia Rita Wijaya Sony Suhandono Tengku Muhammad Ghazali Tengku Muhammad Ghazali Wibowo, Muhammad Arif Wijaya, Rita