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Effect of Breadfruit Leaf (Artocarpus altilis) Extract on Histological Features of the Seminiferous Tubules in Diabetic Wistar Rats Wulandari, Puspa; NurCita, Boenga; Kristanti, Melly; Irsyad, Nasihin Saud
AL-MIKRAJ Jurnal Studi Islam dan Humaniora (E-ISSN 2745-4584) Vol 5 No 01 (2024): Al-Mikraj, Jurnal Studi Islam dan Humaniora
Publisher : Pascasarjana Institut Agama Islam Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37680/almikraj.v5i01.6436

Hyperglycemia can lead to increased free radicals formation that can potentially cause oxidative stress as a trigger for male reproductive organ damage, damaging the histological structure of seminiferous tubules as a place for sperm cell formation. Flavonoids in breadfruit (Artocarpus altilis) are known to be able to counteract free radicals caused by diabetes. The research was conducted to observe the effect of breadfruit leaf extract on the histological features of seminiferous tubules in diabetic Wistar rats. This true-experimental research with post-test-only control group design and purposive sampling technique to obtain 30 Wistar white male rats 8-12 weeks old weighing 150-200 g were grouped into 5 groups. The negative control group was fed standard food; the positive control was induced with STZ, and treatments 1, 2, and 3 were given the extract at doses of 200 mg/kgBW, 400 mg/kgBW, and 800 mg/kgBW consecutively for 30 days. Assessment of spermatogenesis in the histology of seminiferous tubule using Johnsen score criteria. Data were analyzed by the Kruskal-Wallis and the Mann-Whitney U posthoc test, which revealed significance with a p-value of <0.001 (<0.05). This study showed that breadfruit leaf extract can improve the histology of seminiferous tubules. Despite not being as good as the histology of seminiferous tubules under normal conditions, it has a therapeutic effect.

Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract Hasanah, Uswatun; Fauziah, Cut; Irsyad, Nasihin saud; Pramono, Andri Pramesyanti; Wahab, Dinda Hanifah; Nawawi, Wafa Nabila
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1003-1009

Spermatogenesis is the process of spermatozoa formation. An increase in free radicals, such as in hyperglycemia conditions, causes oxidative stress, which can interfere with spermatogenesis. Oxidative stress is an inequality condition between the body’s capacity to produce antioxidants and the rise of free radicals. Antioxidants such as flavonoids are contained in plants such as Moringa oleifera leaves. During spermatogenesis, an apoptosis process involves the BAX gene. BAX is a gene that functions as pro-apoptosis. There is also a CO1 gene in mitochondria, which plays a role in cellular metabolism. Gene expression examination assesses the comparative threshold cycle (CT) with RT-PCR, and spermatozoa quality examination includes assessing spermatozoa concentration and motility. This study used 30 male white Sprague Dawley rats as the test animals, which were divided into five treatment groups: treatment of moringa leaf extract dose 200 mg/kg BW, treatment dose 300 mg/kg BW, treatment dose 400 mg/kg BW, normal control group, and positive control group. The results showed a decrease in BAX gene expression and CO1 gene expression in the treatment group of test animals. The results of sperm analysis showed an increase in spermatozoa concentration in all treatment groups compared to the control group. For sperm motility in the 400 mg/KgBB dose treatment, an increase in sperm motility compared to the control group. This study concludes that administering moringa leaf extract reduced the expression of the Bax and CO1 genes. However, no significant changes were observed in sperm concentration and motility within the treatment group.

Effect of Breadfruit Leaf (Artocarpus altilis) Extract on Histological Features of the Seminiferous Tubules in Diabetic Wistar Rats Wulandari, Puspa; NurCita, Boenga; Kristanti, Melly; Irsyad, Nasihin Saud
AL-MIKRAJ Jurnal Studi Islam dan Humaniora Vol. 5 No. 01 (2024): Al-Mikraj, Jurnal Studi Islam dan Humaniora
Publisher : Pascasarjana Institut Agama Islam Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37680/almikraj.v5i01.6436

Hyperglycemia can lead to increased free radicals formation that can potentially cause oxidative stress as a trigger for male reproductive organ damage, damaging the histological structure of seminiferous tubules as a place for sperm cell formation. Flavonoids in breadfruit (Artocarpus altilis) are known to be able to counteract free radicals caused by diabetes. The research was conducted to observe the effect of breadfruit leaf extract on the histological features of seminiferous tubules in diabetic Wistar rats. This true-experimental research with post-test-only control group design and purposive sampling technique to obtain 30 Wistar white male rats 8-12 weeks old weighing 150-200 g were grouped into 5 groups. The negative control group was fed standard food; the positive control was induced with STZ, and treatments 1, 2, and 3 were given the extract at doses of 200 mg/kgBW, 400 mg/kgBW, and 800 mg/kgBW consecutively for 30 days. Assessment of spermatogenesis in the histology of seminiferous tubule using Johnsen score criteria. Data were analyzed by the Kruskal-Wallis and the Mann-Whitney U posthoc test, which revealed significance with a p-value of <0.001 (<0.05). This study showed that breadfruit leaf extract can improve the histology of seminiferous tubules. Despite not being as good as the histology of seminiferous tubules under normal conditions, it has a therapeutic effect.

RELATIONSHIP BETWEEN BIOLOGICAL ASPECT OF LIFE WITH DEPRESSIVE DISORDERS IN THE ELDERLY AT THE SCHOOL OF ELDERLY Alifio, Anugrah; Hadiwiardjo, Yanti Harjono; Irsyad, Nasihin Saud; Ria Maria Theresa
The Indonesian Journal of Public Health Vol. 20 No. 3 (2025): THE INDONESIAN JOURNAL OF PUBLIC HEALTH
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijph.v20i3.2025.420-434

Introduction: Depressive disorders are one of the most common disorders throughout the world, with a prevalence reaching 5.7% in elders aged over 60 years. In the elderly, depressive disorders can make patients feel miserable, worsen medical conditions, and require a proper support system. Aims: This study aims to explore the relationship between physical activity, cognitive ability, and medical history with depressive disorders in the elderly population in Pondok Labu, South Jakarta, Indonesia. Methods: A cross-sectional quantitative approach was utilized, involving elderly participants from the School of Elderly RPTRA Pinang Pola. Data were gathered via questionnaires, and analyses included univariate and bivariate methods, with the chi-square test assessing relationships between variables. Results: Among the 34 participants, 88.2% showed non-concerning levels of depression, while 11.8% exhibited light depression. Physical activity levels of intensity were light for 11.8%, moderate for 64.7%, and high for 22.3% of participants. Cognitive assessments indicated that 2.9% were below average, 94.1% were normal, and 2.9% were above average cognitive ability. Regarding medical history, 61.8% had up to two diseases, while 38.2% had more than two diseases. Bivariate analyses revealed no significant relationships between depression levels and physical activity (p=0.551), cognitive ability (p=0.118), or medical history (p=0.627). Conclusion: These findings suggest that depression may be influenced by factors beyond those studied here, highlighting the need for broader research on depression determinants in the elderly such as the level of loneliness and the function of family and friends in the elder’s environment.

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