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All Journal Jurnal Biologi Tropis Jurnal Pembelajaran dan Biologi Nukleus Bioeksperimen
Nurhafitri, Amanda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Literature Review: The Potential of Phenolic Compounds as Allelopathic Agents in Mangifera indica Noli, Zozy Aneloi; Nurhafitri, Amanda; Puspita, Ayumi Rizci; Putri, Mellanie Alia; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8271

Abstract

Phenolic compounds are a group of secondary metabolites identified as having significant roles in various ecophysiological processes, including allelopathic activity. In Mangifera indica (mango), phenolic compounds contribute to chemical interactions between plants by releasing allelopathic substances that influence the growth and development of surrounding plant species. This study aims to review the potential of phenolic compounds in M. indica as allelopathic agents, focusing on identifying dominant types of phenolic compounds, allelopathic mechanisms, and their ecological impacts on plant communities. The research method employed is a literature review involving a search for relevant sources on Google Scholar covering the period from 2010 to 2024. Based on a review of various studies, it has been demonstrated that phenolic compounds in M. indica possess significant potential to inhibit the growth of competitors and can be utilized in environmentally friendly agricultural management.
A Literature Review on Transgenic Crops in Indonesia and ASEAN Countries: Transgenic Research Development and Future Prospects for Food Sovereignty Zulkarnain, Alivia; Nurhafitri, Amanda; Tjong, Djong Hon; Idris, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10224

Abstract

The growing global population, climate change, and limited agricultural land are key challenges threatening food security. One innovative solution is the development of transgenic crops, genetically engineered plants that possess superior traits such as resistance to pests, diseases, abiotic stress, and improved nutritional value. This literature review explores the historical development of transgenic research in Indonesia and other ASEAN countries, the technological approaches used—including Agrobacterium-mediated transformation, biolistic methods, and CRISPR/Cas9 - and the future prospects for achieving national food sovereignty. While countries like China and the Philippines have advanced in Genetically Modified Organism (GMO) commercialization, Indonesia remains in the research phase due to various obstacles, including limited expertise, low research funding, regulatory constraints, and public acceptance. Strengthening regulatory frameworks, human resource development, and international collaborations are essential for supporting sustainable agricultural biotechnology. This paper highlights the need for a strategic, science-based approach to integrating transgenic technology into Indonesia's agricultural system as part of its long-term food security and innovation agenda.
Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches Nurhafitri, Amanda; Suwirmen
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16129

Abstract

Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. annuum using integrated in silico and experimental approaches. Primer design was performed using NCBI Primer-BLAST based on the CaPIP reference sequence (XM_016711608.2), followed by in silico evaluation of primer specificity and secondary structure using Primer-BLAST and OligoAnalyzer. Nine primer pairs were initially generated and evaluated based on primer length, %GC, Tm, self3’ complementarity and amplicon size. Secondary structure analysis revealed strong self-dimer formation in pair 8, whereas pair 2 showed weak secondary structure within acceptable ΔG threshold (-9 kcal/mol). Experimental validation was conducted throught gradient PCR to optimize annealing temperature, followed by agarose gel 2%. Primer 2_CaPIP produced specific and clear amplification, with an optimal annealing temperature of 57.3oC. This study provides a validated CaPIP primer set suitable for qPCR-based gene expression analysis in C. annuum, supporting future molecular studies on drought stress tolerance.
Sex-Specific Bands in Dioecious Plant Jernang Rattan (Daemonorops draco) Based on RAPD Markers Nurhafitri, Amanda; Mansyurdin, Mansyurdin; Tjong, Djong Hon; Syamsuardi, Syamsuardi; Asra, Revis; Idris, Muhammad
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.9059

Abstract

Background: Daemonorops draco (Willd.) Blume, or dragon’s blood rattan, is a dioecious species that produces high-value resin but is increasingly threatened by habitat degradation. Effective cultivation and conservation require early identification of male and female plants, which remains challenging at the seedling stage. Although RAPD markers have been used for sex identification in dioecious plants, studies on D. draco are still limited. Therefore, this study aims to screen sex-specific bands in D. draco using the RAPD approach. Methodology: Samples of six male and six female individuals collected from Jambi were analyzed, with DNA isolated using a modified CTAB protocol and amplified using 44 RAPD primers. The products were separated by 2% agarose gel electrophoresis. Bands were considered sex-specific if they consistently appeared only in one sex and were absent in the other. Findings: Out of the 44 primers tested, 39 successfully amplified D. draco DNA, generating a total of 662 bands. Among them, primer OPA-14 (455 bp) and OPC-19 (310 bp) produced male-specific bands that were consistently present in all male individuals, while primer OPB-10 (850 bp) generated a female-specific band detected in all female individuals. The male-specific 455 bp band from OPA-14 and 310 bp from OPC-19, and the female-specific 850 bp band from OPB-10 were identified. These bands are recommended for development into SCAR markers for early sex identification in D. draco. Contributions: The findings support the provision of male and female seedlings to enhance conservation and cultivation programs
Co-Authors . Mansyurdin Djong Hon Tjong Muhammad Idris Puspita, Ayumi Rizci Putra Santoso Putri, Mellanie Alia Revis Asra Suwirmen Syamsuardi Syamsuardi Zozy Aneloi Noli Zulkarnain, Alivia