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All Journal Jurnal Biologi Tropis Bioeksperimen
Nurhafitri, Amanda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Literature Review: The Potential of Phenolic Compounds as Allelopathic Agents in Mangifera indica Noli, Zozy Aneloi; Nurhafitri, Amanda; Puspita, Ayumi Rizci; Putri, Mellanie Alia; Santoso, Putra
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8271

Abstract

Phenolic compounds are a group of secondary metabolites identified as having significant roles in various ecophysiological processes, including allelopathic activity. In Mangifera indica (mango), phenolic compounds contribute to chemical interactions between plants by releasing allelopathic substances that influence the growth and development of surrounding plant species. This study aims to review the potential of phenolic compounds in M. indica as allelopathic agents, focusing on identifying dominant types of phenolic compounds, allelopathic mechanisms, and their ecological impacts on plant communities. The research method employed is a literature review involving a search for relevant sources on Google Scholar covering the period from 2010 to 2024. Based on a review of various studies, it has been demonstrated that phenolic compounds in M. indica possess significant potential to inhibit the growth of competitors and can be utilized in environmentally friendly agricultural management.
A Literature Review on Transgenic Crops in Indonesia and ASEAN Countries: Transgenic Research Development and Future Prospects for Food Sovereignty Zulkarnain, Alivia; Nurhafitri, Amanda; Tjong, Djong Hon; Idris, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10224

Abstract

The growing global population, climate change, and limited agricultural land are key challenges threatening food security. One innovative solution is the development of transgenic crops, genetically engineered plants that possess superior traits such as resistance to pests, diseases, abiotic stress, and improved nutritional value. This literature review explores the historical development of transgenic research in Indonesia and other ASEAN countries, the technological approaches used—including Agrobacterium-mediated transformation, biolistic methods, and CRISPR/Cas9 - and the future prospects for achieving national food sovereignty. While countries like China and the Philippines have advanced in Genetically Modified Organism (GMO) commercialization, Indonesia remains in the research phase due to various obstacles, including limited expertise, low research funding, regulatory constraints, and public acceptance. Strengthening regulatory frameworks, human resource development, and international collaborations are essential for supporting sustainable agricultural biotechnology. This paper highlights the need for a strategic, science-based approach to integrating transgenic technology into Indonesia's agricultural system as part of its long-term food security and innovation agenda.
Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches Nurhafitri, Amanda; Suwirmen
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16129

Abstract

Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. annuum using integrated in silico and experimental approaches. Primer design was performed using NCBI Primer-BLAST based on the CaPIP reference sequence (XM_016711608.2), followed by in silico evaluation of primer specificity and secondary structure using Primer-BLAST and OligoAnalyzer. Nine primer pairs were initially generated and evaluated based on primer length, %GC, Tm, self3’ complementarity and amplicon size. Secondary structure analysis revealed strong self-dimer formation in pair 8, whereas pair 2 showed weak secondary structure within acceptable ΔG threshold (-9 kcal/mol). Experimental validation was conducted throught gradient PCR to optimize annealing temperature, followed by agarose gel 2%. Primer 2_CaPIP produced specific and clear amplification, with an optimal annealing temperature of 57.3oC. This study provides a validated CaPIP primer set suitable for qPCR-based gene expression analysis in C. annuum, supporting future molecular studies on drought stress tolerance.
Co-Authors Djong Hon Tjong Muhammad Idris Puspita, Ayumi Rizci Putra Santoso Putri, Mellanie Alia Suwirmen Zozy Aneloi Noli Zulkarnain, Alivia