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CYTOTOXIC ANALYSIS OF CAPSAICINOID COMPOUND FROM ROTTEN AND FRESH Capsicum frutescens L. ON T47D CELLS Abrilia, Putri Riza; Hayati, Elok Kamilah; Kadarani, Deva Krisna
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 11, No 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.40821

Abstract

Cayenne pepper has a distinctive spicy and hot taste that indicates the presence of capsaicinoid compounds. The content of capsaicinoid compounds is thought to increase along with the level of maturity. This study aims to distinguish between the capsaicinoid content in Rotten Capsicum Frutescens (RCF) and fresh Capsicum Frutescens (FCF) samples and their use as anticancer agents. Capsaicinoid extraction was performed by using reflux extraction and the identification processes using Thin Layer Chromatography (TLC) with a chloroform:methanol eluent. Identification was conducted by using Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) while a cytotoxicity test on T47D breast cancer cells was conducted using the Methylthiazol Tetrazolium (MTT) assay. The results showed the presence of a capsaicinoid compound group, with Retardation Factor (Rf) values of 0.76 in RCF and 0.72 in FCF, as well as orange spots due to its reaction to Dragendorff's reagent. ATR-FTIR analysis was able to identify some of typical functional groups typical of capsaicinoid, including -OH, CH3, CH2 (stretching), C=O, C=C, -CH2- (bending), C-N, C-O, O-CH3, and -NH with the intensity value of RCF greater than FCF. The cytotoxicity test results showed that the Inhibition Concentration (IC50) value for RCF isolates was 512.37 µg/mL, while for FCF isolates was1386.82 µg/mL. These results indicate that capsaicinoid isolates from RCF have a higher cytotoxicity compared to isolates from FCF. A higher IC50 value corresponds to lower cytotoxic activity.
CYTOTOXIC ANALYSIS OF CAPSAICINOID COMPOUND FROM ROTTEN AND FRESH Capsicum frutescens L. ON T47D CELLS Abrilia, Putri Riza; Hayati, Elok Kamilah; Kadarani, Deva Krisna
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 11 No. 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.40821

Abstract

Cayenne pepper has a distinctive spicy and hot taste that indicates the presence of capsaicinoid compounds. The content of capsaicinoid compounds is thought to increase along with the level of maturity. This study aims to distinguish between the capsaicinoid content in Rotten Capsicum Frutescens (RCF) and fresh Capsicum Frutescens (FCF) samples and their use as anticancer agents. Capsaicinoid extraction was performed by using reflux extraction and the identification processes using Thin Layer Chromatography (TLC) with a chloroform:methanol eluent. Identification was conducted by using Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) while a cytotoxicity test on T47D breast cancer cells was conducted using the Methylthiazol Tetrazolium (MTT) assay. The results showed the presence of a capsaicinoid compound group, with Retardation Factor (Rf) values of 0.76 in RCF and 0.72 in FCF, as well as orange spots due to its reaction to Dragendorff's reagent. ATR-FTIR analysis was able to identify some of typical functional groups typical of capsaicinoid, including -OH, CH3, CH2 (stretching), C=O, C=C, -CH2- (bending), C-N, C-O, O-CH3, and -NH with the intensity value of RCF greater than FCF. The cytotoxicity test results showed that the Inhibition Concentration (IC50) value for RCF isolates was 512.37 µg/mL, while for FCF isolates was1386.82 µg/mL. These results indicate that capsaicinoid isolates from RCF have a higher cytotoxicity compared to isolates from FCF. A higher IC50 value corresponds to lower cytotoxic activity.
Evaluasi dan Strategi Biodiesel Sebagai Energi Terbarukan di Indonesia: Literature Review Iklima, Ilma Kanzu; Hidayat, Taupik; Sofinila, Mida Fitri Agasty; Abrilia, Putri Riza; Sanjaya, Eli Hendrik; Utomo, Yudhi
Jurnal Pengendalian Pencemaran Lingkungan (JPPL) Vol. 7 No. 2 (2025): JPPL, September 2025
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat (P3M), Politeknik Negeri Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35970/7ktxek50

Abstract

Biodiesel memiliki potensi besar sebagai energi terbarukan untuk mengurangi ketergantungan Indonesia pada bahan bakar fosil sekaligus menekan emisi gas rumah kaca. Penelitian ini bertujuan mengevaluasi potensi biodiesel di Indonesia melalui kajian literatur terhadap kebijakan, kendala, serta strategi pengembangannya. Metode yang digunakan adalah literature review dengan menelaah 23 artikel terbitan 2015–2025 yang relevan dengan topik biodiesel. Hasil kajian menunjukkan bahwa meskipun kebijakan mandatori B-2,5 hingga B-35 berhasil meningkatkan produksi dan konsumsi biodiesel secara signifikan, implementasinya masih menghadapi empat kendala utama, yaitu ketergantungan pada kelapa sawit sebagai bahan baku, keterbatasan infrastruktur distribusi, hambatan pendanaan akibat biaya produksi tinggi, serta kompleksitas teknologi konversi. Strategi yang diusulkan meliputi diversifikasi bahan baku melalui minyak jelantah, limbah pertanian, dan mikroalga; pengembangan teknologi katalis heterogen dan metode intensifikasi proses (microwave, ultrasonik); optimalisasi subsidi dan instrumen fiskal; pembangunan infrastruktur distribusi di luar Jawa; serta kolaborasi internasional dalam transfer teknologi dan pasar ekspor. Kajian ini menegaskan bahwa dengan integrasi strategi tersebut, biodiesel berpotensi menjadi pilar utama transisi energi berkelanjutan di Indonesia. Kata kunci: Kajian Literatur, Kebijakan Biodiesel di Indonesia, Solusi Energi Keberlanjutan.