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All Journal HAYATI Journal of Biosciences Prosiding Seminar Nasional Pengabdian Kepada Masyarakat
Fera Kurniadewi
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Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Public Health Other

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The pipB Gene as Target for Development of Detection Method of Pathogenic Bacteria Salmonella typhi Using Real-time Polymerase Chain Reaction Nurjayadi, Muktiningsih; Gusti Angieta Putri; Ananda Indah Putri Sihombing; Puan Aqila Azizah; Anisa Fitriyanti; Royna Rahma Musie; Helzi Angelina; Grace; Agus Setiawan; Dandy Akbar Juliansyah; Jefferson Lynford Declan; Gladys Indira Putri; Siti Fatimah; Adinda Myra Amalia Putri; Vira Saamia; Irma Ratna Kartika; Fera Kurniadewi; Shyi-Tien Chen; Bassam Abomoelak; Hesham A. El Enshasy
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.747-757

Abstract

Salmonella typhi is a bacteria that leads to typhoid fever and one of the causes of death due to bacteria infections. In Indonesia, typhoid fever occurs around 1,100 cases per 100,000 population per year, with a mortality rate of 3.1-10.4%. It's necessary to develop a rapid and accurate detection of Salmonella typhi. The pipB gene of Salmonella typhi has the function of being an autophagia inhibitor in humans. This study aims to develop a detection kit for Salmonella typhi pathogenic bacteria targeting the pipB gene using a pipB primer in confirmation, specificity, and sensitivity tests. The results showed that pipB primer can amplify Salmonella typhi DNA fragment of 196 bp at the optimum annealing temperatures between 54-62°C. Confirmation test with real-time PCR found that the pipB primer pair (pipB-F and pipB-R) amplified at cycle 12.93 and 13.10 (Duplo) with a Tm value of 84.05°C and 84.20°C (Duplo). Based on the difference and average value produced in the confirmation and specificity test, pipB primer has amplified Salmonella typhi DNA at Ct 12.47±0.6 with a Tm value of 83.62°C±0.6. The pipB primer pair (pipB-F and pipB-R) could distinguish target from non-target bacteria based on their cycle threshold (Ct) and melting temperature (Tm) values. The primer design of pipB primer pair (pipB-F and pipB-R) successfully detected Salmonella typhi bacteria with the smallest concentration of 55.78 × 102 CFU equivalent to 3.2 pg/µL. Based on the results, Salmonella typhi pipB primer successfully detected Salmonella typhi bacteria DNA rapidly, specifically, and sensitively using the real-time polymerase chain reaction method.
DISEMINASI MODUL ELEKTRONIK KIMIA GENERASI KE- 4 BERBASIS ETNOSAINS DAN ECO-STEAM PADA GURU KIMIA DI MGMP WILAYAH JAKARTA SELATAN 2 Muktiningsih Nurjayadi; Fera Kurniadewi; Irma Ratna Kartika; Siti Fatimah; Nasywa Fhelia Salta; Athiyah Layla; Mellyna Fitriani; Annisa Maharani; Sarah Adilisa Kartini; Nabilla Anisa Putrie; Jefferson Lynford Declan
Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2024): PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT - SNPPM2024
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Negeri Jakarta

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Abstract The rapid advancement of technology has profoundly influenced the development of learning media, accessibility, and the overall quality of education. The 4th generation electronic chemistry module represents an innovative educational tool that integrates visual, audio, audio-visual, and interactive features, facilitating enhanced communicating among learning resources, students, and educators to achieve learning objectives. This Community Service Program (P2M) aimed to disseminate research findings related to the 4th generation electronic module, which incorporates elements of ethnoscience and eco-STEAM. The dissemination activities were conducted with chemistry teachers from the South Jakarta 2 MGMP through a series of informational sessions and workshops. During the informational sessions, the theoretical framework underpinning the module's development was presented, along with an overview of the necessary applications, developmental procedures, and the integration of features such as videos, quizzes, images, pop-ups, and other interactive media tailored to specific instructional models. In the workshop component, participants were guided through the process of creating electronic modules, culminating in the successful production of a standardized prototype. The results showed that approximately 94.7% to 98.9% of participants were satisfied with the training. They found the delivery of the training material to be relevant and as expected, applicable to chemistry learning in schools, and inspiring for new interactive teaching ideas. Participants also felt that the presenter had a strong understanding of the topic, used the allotted time effectively, communicated the material clearly, answered questions well, and ensured that the training ran smoothly. Based on the findings, it is concluded that the dissemination of the 4th generation electronic chemistry module was highly effective. Keywords: The 4th generation Chemistry Electronic Module, Ethnoscience, Eco-STEAM, MGMP Abstrak Perkembangan teknologi yang semakin pesat memberikan dampak signifikan pada pengembangan media, aksesibilitas dan kualitas pembelajaran. Modul elektronik kimia generasi ke-4 merupakan Modul elektronik yang dapat menampilkan secara komprehensif, baik visual, audio, audio-visual, dan aktivitas interaktif antara sumber belajar, peserta didik dan guru untuk mencapai tujuan pembelajaran. Program Pengabdian kepada Masyarakat (P2M) ini bertujuan melakukan diseminasi produk penelitian tentang modul elektronik generasi ke-4 yang diwarnai dengan Etnosains dan Eco-STEAM. Diseminasi dilakukan pada guru-guru di MGMP Kimia wilayah Jakarta Selatan 2 dengan metode penyampaian materi scara diskusi informasi, dan workshop. Pada sesi materi disampaikan teori yang mendasari pengembangan modul, berbagai aplikasi yang dibutuhkan, langkah pengembangan, penambahan fitur video, kuis, gambar, teknik pop up, dan media interaktif lainnya yang dibuat berdasarkan alur model pembelajaran tertentu. Pada tahap workshop peserta diberikan kesempatan dan dibimbing dalam pembuatan elektronik modul sampai menghasilkan prototype standar. Kepuasan dari peserta diukur melalui kuesioner dan tanya jawab, hasilnya menunjukkan bahwa 94,7-98,9% peserta puas dengan penyampaian materi pelatihan, materi yang disampaikan relevan dan sesuai dengan yang diharapkan, dapat diterapkan dalam pembelajaran kimia di sekolah, memunculkan ide-ide baru untuk melaksanakan pembelajaran interaktif di kelas, pemateri sangat memahami topik yang disampaikan, waktu penyampaian materi mencukupi, penyampaian materi sangat baik dan mudah dipahami, pemateri menjawab pertanyaan peserta dengan sangat baik dan pelatihan berlangsung dengan lancar. Berdasarkan hasil yang diperoleh disimpulkan bahwa diseminasi modul elektronik kimia generasi ke-4 telah berhasil dengan sangat baik. Kata Kunci: Modul Elektronik Kimia generasi ke-4, Etnosains, Eco-STEAM, MGMP
Co-Authors Adinda Myra Amalia Putri AGUS SETIAWAN Ananda Indah Putri Sihombing Anisa Fitriyanti Annisa Maharani Athiyah Layla Bassam Abomoelak Dandy Akbar Juliansyah Gladys Indira Putri Grace Gusti Angieta Putri Helzi Angelina Hesham A. El Enshasy Irma Ratna Kartika Jefferson Lynford Declan Mellyna Fitriani Muktiningsih Nurjayadi Nabilla Anisa Putrie Nasywa Fhelia Salta Puan Aqila Azizah Royna Rahma Musie Sarah Adilisa Kartini Shyi-Tien Chen SITI FATIMAH Vira Saamia