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Wulandana, Rachmadian
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Earth & Planetary Sciences Environmental Science Materials Science & Nanotechnology Mathematics Physics

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Identification and antioxidant activity test of β-tocopherol from Dompu corn oil as anti-aging Permadi, Adi; Budiastuti, Pramudita; Setyanto, Barry Nur; Wulandana, Rachmadian; Nuraisyah, Fatma; Novitasari, Putri Rachma; Syamsuddin, Arief; Ramadhan, Muhammad Kaisar Sutomo; Rosandy, Andi Rifki
International Journal of Advances in Applied Sciences Vol 14, No 1: March 2025
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v14.i1.pp175-184

Abstract

Corn is a well-cultivated food crop that is grown extensively worldwide. The used portion of corn is its seeds, which are rich in oil. The objective of this study is to separate and characterize tocopherol from corn oil in Dompu, Sumbawa by employing spectroscopic techniques such as ultraviolet (UV) spectrophotometry, ultra-performance liquid chromatography (UPLC), nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Subsequently, the in-vitro antioxidant activity of the tocopherol was assessed. 1 kilogram of dry maize kernels subjected to 70% ethanol extraction yielded 30 grams (35 ml) of corn oil. The purified isolate obtained from the fractionated extract, using radial chromatography, demonstrated the presence of tocopherol. The isolated sample exhibited the presence of beta-tocopherol. Beta-tocopherol's anti-aging properties were assessed by conducting an in-vitro antioxidant activity test utilizing the tyrosinase enzyme. The IC50 value obtained was 83.954±2.849 ppm. The IC50 value indicates that beta-tocopherol possesses significant antioxidant activity, making it suitable for usage as a primary ingredient in cosmetics and pharmaceutical products.
Co-Authors Adi Permadi, Adi Andi Rifki Rosandy Budiastuti, Pramudita Fatma Nuraisyah, Fatma Novitasari, Putri Rachma Ramadhan, Muhammad Kaisar Sutomo Setyanto, Barry Nur Syamsuddin, Arief