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All Journal IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) IJEMS (Indonesian Journal of Environmental Management and Sustainability) Journal of Natural Sciences Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Indonesian Journal of Chemical Science
Musifa, Eva
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Environmental Science Languange, Linguistic, Communication & Media Nursing Physics Public Health Social Sciences

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The Effect of Oxidative Stress on Growth and Antioxidant Activity of Microalgae Oocystis sp Musifa, Eva; Warni, Mega; Talib, Ramanisa Muliani; Dharma, Abdi; Mardiah, Elida; Harvianti, Yuniar; Indah, Indah; Tania, Alinda
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 1 (2025): February 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i1.30

Abstract

This study was conducted to see the effect of growth and antioxidant activity of microalgae Oocystis sp before and after adding NaOCl to Growmore medium combined with urea. Antioxidant activity test was conducted by calculating IC50 spectrophotometrically with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Oocystis sp extract was obtained by sonication and maceration process with methanol solvent. The results showed the best growth curve for the growth of microalgae Oocystis sp in a mixture of Growmore medium 0.02% b/v and Urea 0.002% b/v. These results also showed a decrease in Oocystis sp growth in a mixture of Growmore medium 0.02% b/v and Urea 0.002% b/v with the lowest growth decrease in the addition of NaOCl with a concentration of 0.01%. The IC50 value of Oocystis sp extract in methanol solvent has high antioxidant activity in Growmore 0.02% b/v + Urea 0.002% b/v medium, which is 55.5862 mg/L. It can be concluded that NaOCl affects the growth and antioxidant activity of microalgae Oocystis sp, but at certain concentrations NaOCl experienced a negative effect.Keywords: Microalgae, Oocytis sp, Oxidative Stress, Growth, Antioxidant
Preparation and Application of Biochar from Areca catechu L. Peel for Malachite Green and Reactive Blue Dyes Removal Palapa, Neza Rahayu; Putra, M. Bagus Kurnia; Musifa, Eva; Yuliasari, Nova; Adawiyah, Robiatul
Indonesian Journal of Environmental Management and Sustainability Vol. 9 No. 1 (2025): March
Publisher : Magister Program of Material Science, Graduate School of Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/ijems.2025.9.1.28-35

Abstract

 The utilization of Areca catechu L. fruit peel waste as biochar offers a sustainable solution for waste management while enhancing the material’s value as an efficient adsorbent. Biochar from Areca catechu L. fruit peels was processed through pyrolysis for 2 hours at temperature of 500oC. This study investigates the use of biochar from Areca catechu L. peel as an adsorbent for Malachite Green (MG) and Reactive Blue (RB) dyes in a batch system, with a focus on analysing the kinetic adsorption processes. The biochar was characterized using XRD, FTIR, SEM and BET. BET analysis was conducted, which shows that the surface area of biochar from Areca catheca L. fruit peel after pyrolysis treatment was 29.52 m2/g. The adsorption of MG by the biochar from Areca catechu L. peels was found to be greater than that of RB. The biochar showed an adsorption capacity of 16.113 mg/g for MG, while its capacity for RB was 15.499 mg/g. The adsorption process of biochar from Areca catechu L. peels for both MG and RB dyes follows a pseudo-second-order kinetic model.
Penyediaan Obat Penyakit Kulit Dan Luka Dari Cairan Fermentasi Lidah Buaya Dan Daun Sirih Riyanti, Fahma; Desnelli, Desnelli; Fatma, Fatma; Yuliasari, Nova; Julinar, Julinar; Musifa, Eva
Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Vol. 5 No. 3 (2025): Mei 2025 - Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Publisher : Indonesian Scientific Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59395/altifani.v5i3.693

Abstract

Cairan Fermentasi anaerob pada lidah buaya dan daun sirih mengandung enzim-enzim, beberapa senyawa kimia, dan sumber antimikroba alami. Kandungan ini memiliki kemampuan terhadap antibakteri dan anti jamur yang bermanfaat untuk mengobati penyakit kulit, luka dan untuk kesehatan kulit. Proses penyembuhan dapat dipercepat karena senyawa antibakteri menstimulator respon imun tubuh sehingga dapat menyembuhkan luka dan penyakit kulit. Kegiatan pengabdian yang berupa penyuluhan, praktek pembuatan cairan fermentasi lidah buaya - daun sirih dan pemberian produk yang sudah jadi merupakan metode yang dilaksanakan di Desa Permata Baru Kecamatan Indralaya Utara Kabupaten Ogan Ilir. Agar peserta penyuluhan memahami tujuan kegiatan maka dilakukan praktek pembuatan cairan fermentasi dan peserta penyuluhan yang dibagi menjadi 3 kelompok. Pelaksanaan kegiatan dilaksanakan sebanyak tiga kali. Pelaksanaan kedua adalah memantau cairan fermentasi yang dibuat sebelumnya. Hasil fermentasi dibuka setelah 3 bulan, ketiga cairan fermentasi tersebut berhasil dibuat ditandai aroma asam segar dan warna coklat.
Perbandingan Pertumbuhan dan Produksi Karotenoid pada Mikroalga Chlorella Vulgaris dan Chlorella Pyrenoidosa Musifa, Eva
Journal of Natural Sciences Vol 6, No 3 (2025): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i3.999

Abstract

Microalgae are a potential source of biological biomass due to their ability to produce various bioactive compounds. This study aims to analyze the growth curves and carotenoid content of two microalgae Chlorella Vulgaris and Chlorella Pyrenoidosa, to evaluate their potential as natural carotenoid sources. Microalgae cultures were cultivated in two different types of media, namely F1/2 Guillard and Walne. Growth monitoring was carried out by measuring dry biomass and cell density at a wavelength of 680 nm, while carotenoid content was analyzed spectrophotometrically at wavelengths of 470, 652, and 665 nm. All experiments were carried out in triplicate (n = 3) to obtain representative. The results showed that both species have distinctive growth patterns with different logarithmic phases. Chlorella Vulgaris showed a higher growth rate than Chlorella Pyrenoidosa, namely 0.80 ± 0.013 g/L and 0.43 ± 0.002 g/L for each microalgae. In addition, Guillard's F1/2 medium was proven to be more effective in supporting microalgae growth and carotenoid production in both microalgae strains, namely 0.653±0.018 µg/g for Chlorella Vulgaris and 0.436±0.049 µg/g for Chlorella Pyrenoidosa with a sig p value 0.05. These findings indicate that the selection of appropriate species and culture medium greatly influences microalgae productivity.
Ecoenzyme Characterization of Fruit Peel Waste Mixture and Test of Antibacterial Activity against Bacteria Causing Dental Caries Julinar, Julinar; Uswati, Dinii; Riyanti, Fahma; Musifa, Eva
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 3 (2025): October 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Ecoenzyme is a product of facultative aerobic fermentation for 3 months from organic fruit and vegetable waste. In this study, ecoenzyme was made from a mixture of fruit peel waste (papaya, orange and pineapple) with the addition of 6 liters of water. Ecoenzyme products were characterized by organoleptic properties, chemical composition, enzyme activity and antibacterial activity tests against Lactobacillus acidophilus and Streptococcus mutans bacteria that cause dental caries. The results of the study show that the ecoenzyme product had a dominant orange peel sour flavor, was cloudy brown in color with a volume of 8.4 liters from 6 liters of added water. The characterization results depict that the chemical composition of ecoenzyme contained acetic acid and lactic acid with a total acid content of 2.26%, 40.45 mg/mL protein, and secondary metabolites in the form of alkaloids, flavonoids, and saponins. The activity of several enzymes from ecoenzyme was protease 0.0246 U/mL, amylase 0.0032 U/mL and lipase 5 U/mL. Antibacterial activity against bacteria that cause dental caries is included in the category of very strong against S. mutans at a concentration of 40 (% v/v) and moderate against L. acidophilus with inhibition zone diameters of 23.33 mm and 8.23 mm respectively. The Mininum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MKC) values of ecoenzyme against S. mutans and L. acidophilus bacteria were 2500 ppm and 5000 ppm respectively.Keywords: ecoenzyme, fruit-peel waste, L.acidophilus, S.mutans, antibacterial
Processing of Tofu industrial wastewater by electrocoagulation method using aluminum (Al) electrode at various electrical current and contact time Rachmat, Addy; Fatma, Fatma; Pratama, Nisa Manora; Musifa, Eva
Indonesian Journal of Chemical Science Vol. 14 No. 1 (2025): Indonesian Journal of Chemical Science
Publisher : Prodi Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v14i1.18787

Abstract

This research aims to determine the effect of varying voltage and contact time on reducing levels on Chemical Oxygen Demand (COD), Total Suspended Solids (TSS), Total Dissolved Solids (TDS) and ammonia as well as increasing pH values ​​in tofu industrial wastewater by electrocoagulation using Aluminum electrodes. There were 4 variations of voltage (10,20,30,4 0A) and 5 variations of contact time (30,60,90,120,150 minutes). Analysis of COD levels and ammonia levels was carried out using a UV-Vis spectrophotometer, analysis of TSS levels gravimetrically using Whatman filter paper No. 42, analysis of TDS levels with gravimetrically, and analysis of pH values ​​by a pH meter. The best results were obtained from treatment at voltage of 40 A and a contact time of 150 minutes with the highest efficiency of reducing COD, TSS, TDS, ammonia levels respectively at 90.35; 92; 66; 85% and the highest increase in pH value was 108%. The results of the diversity analysis or Tukey's advanced test showed that at the 5% level each treatment had a significant effect on reducing COD, TSS, TDS and ammonia levels as well as increasing the pH value. Chemical kinetics analysis of the reduction in COD and TSS levels showed results that were close to first orde linear and obtained R2 and k values ​​respectively of (0.9904; 0.0164); (0.9952; 0.0182) at voltage variation of 40 A
Study of Congo Red Adsorption by Chitosan-Graphene Oxide (Chitosan-GO) Composite Synthesize with Hydrothermal Synthetic Method: Optimization and Determination Condition Desnelli, Desnelli; Safitri, Siska; Musifa, Eva; Hamidah, Afreni; Suheryanto, Suheryanto; Mara, Ady; Said, Muhammad
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 2 (2025): June 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i2.101

Abstract

This research aims to synthesize chitosan-graphene oxide (chitosan-GO) material using the hydrothermal method at varying material ratios and adsorption studies on Congo red. Chitosan-GO composites were synthesized with mass ratios of (1:1), (1:3) and (1:5). The synthesized chitosan-GO composite was applied to absorb Congo red dye. The adsorption process is carried out by varying pH, contact time, concentration and temperature. The chitosan-GO composite was characterized using XRD, BET and SEM-EDS. The results of XRD characterization of the chitosan-GO composite with a ratio of 1:1 showed a diffractogram of 2θ = 22.3°, at a ratio of 1:3 the diffractogram angle was 2θ = 22.1°, while the ratio (1:5) of the diffractogram peak obtained was 2θ = 22.8°. The surface area obtained in chitosan-GO composite was 11.60 m2/g. The morphology of the chitosan-GO composite (1:5) is in the form of crystalline lumps with the constituent elements C (65.98%), O (32.68%), Na (0.22%) and S (0.09%). Adsorption of Congo red dye using chitosan-GO composite obtained optimum conditions at pH 7, contact time 50 minutes, Congo red concentration 45 mg/L and temperature 50°C. The adsorption isotherm is in accordance with the Langmuir isotherm model and the maximum adsorption capacity (Qm) is 10.245 mg/g.
Microalgae-Based Wastewater Treatment as Green Solution for Sustainable Degradation and Its Challenges: A Review Musifa, Eva; Palapa, Neza Rahayu; Ahadito, Bijak Riyandi
Indonesian Journal of Environmental Management and Sustainability Vol. 9 No. 4 (2025): December
Publisher : Magister Program of Material Science, Graduate School of Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/ijems.2025.9.4.171-182

Abstract

Releasing polluted water directly into the environment causes serious problems because it contains harmful substances that can damage aquatic life and human health. To reduce these risks, it is important to treat and clean wastewater effectively. Microalgae have shown promise as a natural and sustainable way to treat wastewater. The microalgae help to remove pollutants and can also recover useful resources and energy from the water. The study used a systematic review methodology to identify and evaluate the role of microalgae wastewater treatment. The method was used since it provides a structures and transparent framework for selection, assessment and synthesize of evidence from relevant literature. This review highlights the benefits of using microalgae for wastewater treatment, as well as the challenges involved. Ongoing research focuses on improving these methods, including combining microalgae with bacteria to make the process more efficient and scalable. Despite some difficulties like contamination risks and regulatory issues, future advances especially in genetic engineering and hybrid systems are expected to improve microalgae-based wastewater treatment and make it a key tool for protecting the environment, while multidisciplinary collaboration combining molecular biology research, environmental engineering, public policy, and utilization of this technology at the industrial and societal levels that should be under consideration.
Co-Authors Abdi Dharma Addy Rachmat Ady Mara, Ady Afreni Hamidah Bijak Riyandi Ahadito Desnelli Desnelli Elida Mardiah Fahma Riyanti Fatma Harvianti, Yuniar Indah Indah Julinar Julinar MUHAMMAD SAID Nova Yuliasari Pratama, Nisa Manora Putra, M. Bagus Kurnia Robiatul Adawiyah Safitri, Siska Suheyanto Suheryanto Talib, Ramanisa Muliani Tania, Alinda Uswati, Dinii Warni, Mega