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All Journal BIO PALEMBANICA Journal of Biotropical Research and Nature Technology
Decenly
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Establishment of A Transient Expression Using PEG-Mediated Protoplast Transformation System in Black Rice Cempo Ireng Rahayu Opi Anggoro; Decenly; Yosua Hambit; Nanang Wahyu Prajaka
Journal of Biotropical Research and Nature Technology Vol. 2 No. 1 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i1.11019

Abstract

Indonesia black rice is a potential crop which consider to be develop as functional food because of high nutritional values. However, some agronomical traits, such as high culm and long harvesting time need to be improved for high productivity. Genetic engineering based on protoplast system is one of tools that can be used for improving black rice agronomical traits. The purpose of this study was to establish an efficient method for obtaining protoplasts, and to get information on whether the PEG-mediated transformation method can be carried out on black rice ‘Cempo Ireng’ using GFP transient expression as a marker. To get protoplast culture, we used callus and seedling as main explants. The results showed that 15th days seedling was the best explant source to get protoplast compare to callus. The combination of 1,5% macerozyme and 3% celulase was optimum to obtain the viable protoplasts. Transient expression of GFP can be done using PEG-mediated protoplast transformation in 30% concentration of PEG.
Studi Budidaya Jamur Kuping (Auricularia auricula) dengan Variasi Jenis Substrat dan Konsentrasi Supplemen Yachya, Arif; Sulistyowati; Fatiqin, Awalul; Lestari, Ria Windi; Fitriah, Umi Novita; Decenly; Anggoro, Rahayu Opi
Journal of Biotropical Research and Nature Technology Vol. 1 No. 1 (2022): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i1.7329

Abstract

Terbatasnya stok serbuk gergaji kayu sengon sebagai bahan baku baglog dapat mempengaruhi kelangsungan produksi jamur kuping (Auricularia auricula). Pemanfaatan limbah pertanian sebagai bahan baku alternatif baglog jamur kuping belum banyak diungkap. Ketiga jenis limbah pertanian yang tersedia melimpah di dataran rendah, yaitu jerami padi (PJP), potongan ampas tebu (PAT) dan serbuk gergaji kayu kelapa (SKK). Peforma pertumbuhan dan hasil panen jamur kuping pada ketiga jenis limbah pertanian tersebut yang dikombinasikan dengan dedak  (0, 5, 10 dan 15%) diinvestigasi pada penelitian ini. Serbuk kayu sengon (SKS) digunakan sebagai kontrol. Hasil menunjukkan bahwa waktu tercepat miselium penuh, pembentukan pinhead dan panen pertama dicapai berturut-turut pada hari ke-17-20, 29-32, dan 39-40 inkubasi oleh baglog SKK. Pengamatan pada hasil panen menunjukkan PJP adalah substrat terbaik dibanding SKS (sebagai substrat kontrol), SKK dan PAT. Sebaliknya, peningkatan konsentrasi dedak berdampak negatif pada hasil panen baglog PJP. Performa hasil panen terbaik diperoleh pada baglog PJP dengan dedak 0 % yaitu 13,67 g (berat basah) dengan berat baglog 240 g (berat basah). Hasil ini lebih tinggi 64,11 % dari hasil panen baglog SKS dengan dedak 5-10%. Pada akhirnya, hasil studi ini merekomendasikan PJP sebagai substrat alternatif pengganti SKS.
Keanekaragaman Spesies Kupu-kupu (Lepidoptera) pada Habitat Ekowisata Kawasan Hutan Desa Tahawa Kabupaten Pulang Pisau Tuju, Fandi; Fatiqin, Awalul; Kadafi, Ahmad Muammar; Decenly; Dirgantara, Made; Ulaan, Magdalin; Nugroho, Yuli
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8976

Abstract

Butterflies are part of biodiversity that is important to be preserved because the presence of butterflies in an ecosystem, especially forests, is very important, both as part of the food chain, pollinators, and food for other animals. This study aims to analyze the species diversity and abundance of butterflies (Lepidoptera) in the habitat of Tahawa Village Forest Area, Pulang Pisau Regency. The research method used an exploratory survey method with a sweeping technique following a transect line and then calculated Shannon-Wiener Diversity Index (H'), Evenness Index (E), and Species Richness Index (R1). Butterflies found in the Tahawa Village Forest Area consisted of 6 families divided into 15 species with a total of 63 individuals. The most species found came from the Pieridae family and the least from the Geometridae family. The Diversity Index (H') obtained a value of 2.38, classified as moderate diversity. The Evenness Index (E) is in the high category with 0.88, while the Richness Index (R1) is at 3.38 which is still classified as low.
Long-tailed Macaques Macaca fascicularis (Simiiformes: Cercopithecidae) Coexistence with Settlements in the Nyaru Menteng Arboretum Area, Central Kalimantan Hasanah, Rizka; Purnaningsih, Titin; Anggoro, Rahayu Opi; Decenly; Syafutra, Randy
BIO PALEMBANICA Vol 2 No 1 (2025): Bio Palembanica
Publisher : Lembaga Penelitian, Pengabdian Kepada Masyarakat dan Kepustakaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36982/bio.v2i1.5529

Abstract

The Nyaru Menteng Arboretum area in Central Kalimantan serves as a habitat for approximately 200 Long-Tailed Macaques Macaca fascicularis within its 65.2-hectare area, as recorded in 2024. A lack of food causes presumed M. fascicularis to descend to settlements in search of food, resulting in losses for the people of Nyaru Menteng Arboretum which leads to coexistence. This study aims to identify the types of coexistences that occur between M. fascicularis and villagers. The method used to identify coexistences is a survey method. The survey results explain that the types of coexistences that occur range from stealing side dishes (25%), damaging ceilings (25%) and messing up the kitchen (50%). Human resistance in the form of repellent using slingshots 32%, wood 40%, nets 20% and air rifles 8%. The findings of this study can serve as a reference for managing future coexistences and inform decision-making for effective M. fascicularis management in the Menteng Arboretum Area, Central Kalimantan.   Keywords: Endangered, Indonesian Borneo, interaction, Long-Tailed Monkey, Macaca fascicularis.
Co-Authors Ahmad Muammar Kadafi Anggoro, Rahayu Opi Arif Yachya Fandi Tuju Fatiqin, Awalul Hasanah, Rizka Made Dirgantara Magdalin Ulaan Prajaka, Nanang Wahyu Rahayu Opi Anggoro Ria Windi Lestari Sulistyowati Syafutra, Randy Titin Purnaningsih Yosua Hambit Yuli Nugroho, Yuli