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All Journal e-Journal Pustaka Kesehatan Community Empowerment Jurnal Pengabdian Masyarakat Indonesia Archives Pharmacia Jurnal An-Najat: Jurnal Ilmu Farmasi dan Kesehatan
Putu Gita Maya Widyaswari Mahayasih
Universitas Esa Unggul, Jakarta Barat, Indonesia
Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Journal : e-Journal Pustaka Kesehatan

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Uji Aktivitas Protein Larut Air Umbi Porang (Amorphophallus muelleri Blume) terhadap Escherichia coli dan Staphylococcus aureus Putu Gita Maya Widyaswari Mahayasih; Tri Handoyo; Mochammad Amrun Hidayat
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The objective of this study was to determine antibacterial activity of water soluble protein of Porang (Amorphalus muelleri Blume) tuber. The water soluble protein extract was tested against both Eschericia coli and Staphylococcus aureus using filter paper disk method. The inhibition zone of bacterial growth was calculated for both of bacterial cultures. It was found that water soluble protein exhibited antibacterial activity at linear range 0.3-1.9 μg and 0.1-1.9 μg for E. coli and S. aureus respectively, sugesting that S. aureuswas more susceptible than E. coli to this protein. From the electrophoretic profile using 12.5% sodium dodecyl sulfate (SDS) and comassie blue staining (CBS), two protein bands were observed with molecular weight 17 and 19 kDa respectively. The water soluble protein were further evaluated using periodic acid staining (PAS). Pink band with molecular weight 250 kDa and > 250 kDa was found in PAS profile, sugesting the availability of carbohydrate molecule in water soluble protein extract of porang tubers.
Co-Authors Abna, Inherni Marti Ade Novitasari Boediono, Eddy Purwoto De Vita Enjelin Devin Winata Eden, Yonatan Harizal Lena, Ayu Puspita Listi Maslia Putri Megawati Mochammad Amrun Hidayat Nur Lisa Anggraini Nurdiyanti, Nisa Pertiwi, Ratih Dyah Putri Nuraini Sutomo Putri, Listi Maslia Rafi Perdana Tamher Rolobessy, Amalia Sari, Rohaniva Yusnia Silviana Dewi Siti Nurfitria Sri Teguh Rahayu, Sri Teguh TRI HANDOYO Tria Saras Pertiwi Tyas Putri Utami Vallenchesa Susanto Theo Wahyu Darmawan Wilson Gunawan Yulia Katuuk