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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya JIMR - Journal of Islamic Medicine Research Jurnal Kesehatan Islam : Islamic Health Journal Jurnal Kedokteran Komunitas (Journal of Community Medicine) Jurnal Kedokteran Hewan Journal of Tropical Pharmacy and Chemistry
Doti Wahyuningsih
Fakultas Kedokteran, Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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STUDI PUSTAKA SISTEMATIS: PENGARUH KURKUMIN SEBAGAI ANTIINFLAMASI TERHADAP C-REACTIVE PROTEIN PADA BERBAGAI PENYAKIT INFLAMASI Andrie Devin Susilo Hadi; Marindra Firmansyah; Doti Wahyuningsih
Jurnal Kedokteran Komunitas Vol 9, No 1 (2021)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Abstract: C-Reactive Protein (CRP) merupakan protein yang dihasilkan pada fase akut dari kondisi inflamasi yang sampai sekarang digunakan sebagai penanda sistemik di klinis. Kurkumin dilaporkan memiliki pengaruh sebagai antiinflamasi. Studi Pustaka sistematis ini dilakukan untuk mempelajari pengaruh kurkumin terhadap penurunan kadar CRP pada berbagai kelainan yang patofisiologinya melibatkan inflamasi. Pada studi ini dipela-jari pengaruh kurkumin pada CRP dari kelainan: metabolisme, keganasan, infeksi,dan intoksikasi. Studi Pustaka sistematis. Data dikumpulkan dari PubMed dan Google Scholar berdasarkan kata kunci Curcumin, C-Reactive Protein, dan Inflamation. Proses screening menghasilkan 16 artikel yang memenuhi kriteria inklusi yang ditetapkan untuk ditelaah. Kurkumin terbukti mampu menurunkan kadar CRP pada berbagai kondisi inflamasi. Dalam penelitian ini dilaporkan Kurkumin bekerja dengan menghambat Tumor Necrosis Factor-α (TNF-α), In-terleukin-1β (IL-1β), Interleukin-6 (IL-6) serta menghambat aktivasi faktor transkripsi Nuclear Factor-Kappa B (NF-κB). Sehingga kurkumin mampu menurunkan kadar CRP pada berbagai kondisi inflamasi.Kata Kunci : Kurkumin, C-Reactive Protein, Inflamasi
Pengaruh Kombinasi Dekokta Rimpang Zingiber officinale var rubrum dan Imperata cylindrica Terhadap Kadar MMP-9 Serum Tikus Wistar Jantan Model Osteoartritis Adam Bregas Regowo; Merlita Herbani; Doti Wahyuningsih
Jurnal Kedokteran Komunitas Vol 8, No 1 (2020)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Introduction: Osteoarthritis is a damage chronic disorder caused by degenerative process and inflammation that lead to the joint cartilage. Previous research showed that red ginger rhizome (Zingiber officinale var rubrum) and cogon grass rhizome (Imperata Cylindrica) had anti-inflammation and antioxidant effects. This research aimed to know the potencial of the combination of red ginger rhizome (Zingiber officinale var rubrum) and cogon grass rhizome (Imperata Cylindrica) (CRGCG) by measuring the OA rats serum Matrix Metalloproteinase-9 (MMP-9). Matrix Metalloproteinase-9 is an enzyme that plays a role in joint cartilage destruction.Methods: 25 male Wistar rats age 4 months divided into 5 groups namely positive control group (PC), negative control group (NC), treatment group 1 recieved dose 9+40 mg/rat/day (T1), treatment group 2 recieve dose 18+40 mg/rat/day (T2), and treatment group 3 recieve dose 36+40 mg/rat/day(T3). Rats were induced with Complete Freund’s Adjuvant (CFA) by 0.1 ml/rat on right knee intraarticular to induce. The administration of CRGCG is given for 10 days. The serum MMP-9 level was assessed by MMP-9 ELISA Kit. Data analyzed using One Way Anova followed by Post Hoc test. The result is significant if p<0.05.Result: The Combination of Red Ginger Rhizome and Cogon Grass Rhizome (CRGCG) on the first treatment (0,85±0,03), second treatment (0,86±0,02) and third treatment (0,84±0,02) able to decrease the serum MMP-9 level significantly (p<0,05).Conclusion: The optimal dose from Combination of Red Ginger Rhizome and Cogon Grass Rhizome (CRGCG) to reduce serum MMP-9 level is  dose 9+40 mg/rat/day.Keywords: Cogon Grass, Matrix Metalloproteinase-9, Osteoarthritis, Red Ginger
SYSTEMATIC LITERATURE REVIEW : PENGARUH DELIMA (Punica granatum L.) TERHADAP KADAR MATRIX METALLOPROTEINASE-9 PADA KONDISI INFLAMASI Fidurroty Baidho; Yoyon Arif Martino; Doti Wahyuningsih
Jurnal Kedokteran Komunitas Vol 9, No 1 (2021)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Pendahuluan: Matrix Metalloproteinase-9 (MMP-9) merupakan suatu enzim yang dilaporkan meningkat pada keadaan radang akut maupun kronis. Beberapa penelitian melaporkan, peningkatan kadar MMP-9 dalam tubuh akan menyebabkan komplikasi pada organ vital. Delima (Punica granatum L.) dilaporkan memiliki sifat anti inflamasi sehingga mampu menurunkan kadar MMP-9. Zat aktif ellagitannin, punicalagin, punicalin, ellagic acid, punicid acid dan luteolin dilaporkan mampu menurunkan kadar MMP-9 pada kondisi inflamasi. Tinjauan ini melaporkan pengaruh delima terhadap kadar MMP-9 pada kondisi inflamasi akibat infeksi dan non infeksi dalam penelitian in vivo dan in vitro.Metode: Systematic literature review mengenai pengaruh delima terhadap kadar MMP-9 pada kondisi inflamasi dilakukan dengan melakukan pencarian data pada Google Scholar, PubMed Central, dan PubMed. Kata kunci yang digunakan adalah “punica granatum AND inflammation AND MMP-9”. Dari 953 jurnal, sebanyak 931 jurnal dikecualikan dan terpilih 22 jurnal sesuai dengan kriteria inklusi yang telah ditetapkan antara lain tahun terbit antara 2007-2020, original article, terindeks SCOPUS dan dapat diakses secara full text.Hasil: Zat aktif delima ellagitannins, punicalagin, punicalin, ellagic acid, punicid acid, dan luteolin dilaporkan mampu menurunkan kadar MMP-9 pada kondisi inflamasi. Mekanisme hambatannya yaitu hambatan pada jalur NF-kB, jalur TIMPs, jalur MAPK dan jalur PI3K, serta mampu menurunkan sekresi mediator inflamasi TNF-α dan memicu terjadinya autofagi dan apoptosis.Kata Kunci: Punica granatum, Inflammation, MMP-9
STUDI PUSTAKA SISTEMATIS: DELIMA MEMPERBAIKI KADAR NITRIC OXIDE PADA BERBAGAI KONDISI STRES OKSIDATIF Siti Norazizah; Nugroho Wibisono; Doti Wahyuningsih
Jurnal Kedokteran Komunitas Vol 9, No 1 (2021)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Abstrak : Nitric Oxide (NO) merupakan suatu radikal bebas. Peningkatan kadar NO didapatkan pada berbagai kondisi stres oksidatif. Pemberian ekstrak delima dilaporkan dapat memperbaiki kadar NO dalam kondisi stres oksidatif karena adanya kandungan antioksidan pada delima terutama senyawa polifenol. Penelitian menggunakan metode studi pustaka sistematis bertujuan mengetahui pengaruh delima (Punica granatum) terhadap kadar Nitric Oxide (NO) pada serum, plasma, jaringan hepar, otak, limfe, usus, ginjal dan endotel yang diperoleh dari penyakit yang patofisiologinya melibatkan stres oksidatif (injury, toksisitas, inflamasi dan infeksi). Bagian delima yang dipelajari pada penelitian ini adalah buah, kulit, biji dan bunga. Studi pustaka sistematis. Pengumpulan data penelitian dilakukan melalui sumber PubMed, dan Google Scholar dengan kata kunci Punica granatum, Antioxidant, dan Nitric Oxide. Melalui proses screening didapatkan data berupa 14 artikel memenuhi kriteria inklusi yang telah ditetapkan. Senyawa antioksidan yang terdapat dalam ekstrak buah, kulit, bunga, dan biji delima terbukti mampu memperbaiki nilai NO dengan menurunkan kadar NO pada jaringan hepar, otak, usus, limfe, ginjal dan meningkatkan NO pada jaringan endotel pada kondisi stres oksidatif. Melalui 14 jurnal yang ditelaah, mekanisme antioksidan utama ekstrak delima dalam memperbaiki nilai NO adalah melalui peningkatan antioksidan dan pembersihan radikal bebas. Pemberian ekstrak buah, biji, kulit, dan bunga delima (Punica granatum) dapat memperbaiki kadar radikal NO pada kondisi stres oksidatif.Kata Kunci : Punica granatum, Antioxidant, Nitric Oxide
STUDI PUSTAKA SISTEMATIS: PENGARUH DELIMA TERHADAP SUPEROXIDE DISMUTASE DAN MALONDIALDEHYDE DALAM KONDISI STRES OKSIDATIF Dewi Intan Putri Dwiyanti; Fifin Pradina Duhitatrissari; Doti Wahyuningsih
Jurnal Kedokteran Komunitas Vol 9, No 1 (2021)
Publisher : Jurnal Kedokteran Komunitas (Journal of Community Medicine)

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Abstract

Abstrak: Stres Oksidatif adalah keadaan ketidakseimbangan antara jumlah radikal bebas dan anti-oksidan. Delima (Punica Granatum L.) dilaporkan berperan sebagai scavenger bagi radikal bebas, sehingga dapat mengurangi stres oksidatif. Tujuan dari penelitian studi pustaka sistematis ini ada-lah untuk membuktikan hasil riset tentang kegunaan buah delima dalam mengatasi stres oksidatif dengan memeriksa kadar SOD dan MDA. Kondisi stres oksidatif dalam studi literature ini meliputi intoksisikasi karena penggunaan obat tertentu, diabetes militus, penyakit neurodegenerative dan polutan. Jenis ekstrak yang akan dipelajari adalah ekstark kulit, buah, bunga, daun, biji dan jus buah delima. Systematic Literature Review. Data penelitian dikumpulkan dari PubMed Central, PubMed, dan Google Scholar berdasarkan kata kunci pomegranate, stres oksidatif, SOD, dan MDA. Proses screening menghasilkan 100 artikel yang relevan. Yang memenuhi kriteria inklusi untuk ditelaah berjumlah 17 artikel. Ekstrak kulit, biji, bunga, daun, serta jus buah delima mampu menaikkan kadar SOD dan menurunkan kadar MDA. Jadi ekstrak dari delima mampu menaikkan kadar SOD dan menurunkan kadar MDAKata kunci: Delima, stres oksidatif, SOD dan MDA
STUDY OF SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE CONCENTRATIONS IN MICE AFTER ADMINISTRATION OF METHANOLIC EXTRACT of Scurrula atropurpurea (BL.) Nour Athiroh; Doti Wahyuningsih
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.205 KB) | DOI: 10.21157/j.ked.hewan.v11i1.5431

Abstract

The aim of this research was to investigate the potential effect of methanolic extract of Scurrula atropurpurea (MESA) in reducing oxidative stress through observation of superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration in mice. In this research, 25 male mice were divided into four groups with five replications. Group I (K1, without MESA) was considered as a control group, while Group II (K2), III (K3), and IV (K4) were given 1000, 2000, and 4000 mg MESA/kg BW, respectively. After 14 days, SOD activity and MDA concentration were measured spectrophotometrically. The SOD activity did not significantly increased (P0.05) in group K1, K2, K3, and K4 with values were 19.469±2.072, 17.4±1.373, 20.53±3.411, and 23.87±2.528 U/ml, respectively. In contrast, MDA concentration was decreased (P0.05) with values of 492.750±19.418, 492.750±19.418, 457.125±30.095, 454.625±19.080, and 475.875±37.659 ng/mL in groups K1, K2, K3, and K4, respectively. It can be concluded that the administration of  MESA tend to increase SOD activity and decrease MDA concentration in this study, thus might be able to reduce  oxidative stress in mice.
In Silico Study of Pulutan (Urena lobata) Leaf Extract as Anti Inflammation and their ADME Prediction Doti Wahyuningsih; Yudi Purnomo; Andri Tilaqza
Journal of Tropical Pharmacy and Chemistry Vol. 6 No. 1 (2022): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v6i1.323

Abstract

Inflammation is the basic for the pathogenesis of several diseases both of degenerative and non-degenerative disease. Urena lobata is a medicinal plant that can be found in Indonesia and has been used traditionally to cure influenza, inflammation and fever. However, there have been few reports about their anti-inflammatory activity and their mechanism action are still unclear. The aim of study to evaluate the anti-inflammatory activities of active substances from U.lobata leaf and their pharmacokinetic property through in silico study. lobata leaf was extracted by digeration methods using ethanol solvent. Therefore, the active substances in the extract was analyzed by Liquid Chromatography-Mass Spectra (LC-MS). Pharmacokinetic property and physicochemical of active compounds were evaluated using pkCSM online tool. Anti-inflammatory activity of U. lobata active compound on phospholipase-A2 (PLA-2), cyclooxygenase-2 (COX-2) and lipoxygenase-5 (LOX-5) were evaluated by in silico study. Ethanolic extract of U. lobata contained five active compound, there are stigmasterol, ?-sitosterol mangiferin, gossypetin and chrysoeriol. Molecular docking study indicated stigmasterol and ?-sitosterol of U. lobata have a strong activity as anti-inflammatory based on the estimation of inhibition constant (Ki) value against PLA2 and COX-2. Meanwhile, mangiferin and gossypetin have a stronger anti-inflammatory effect on LOX-5 among others compound. U. lobata has anti-inflammatory activity through inhibition on COX-2 greater than on PLA2 and LOX-5.
STUDY OF SUPEROXIDE DISMUTASE AND MALONDIALDEHYDE CONCENTRATIONS IN MICE AFTER ADMINISTRATION OF METHANOLIC EXTRACT of Scurrula atropurpurea (BL.) Athiroh, Nour; Wahyuningsih, Doti
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5431

Abstract

The aim of this research was to investigate the potential effect of methanolic extract of Scurrula atropurpurea (MESA) in reducing oxidative stress through observation of superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration in mice. In this research, 25 male mice were divided into four groups with five replications. Group I (K1, without MESA) was considered as a control group, while Group II (K2), III (K3), and IV (K4) were given 1000, 2000, and 4000 mg MESA/kg BW, respectively. After 14 days, SOD activity and MDA concentration were measured spectrophotometrically. The SOD activity did not significantly increased (P0.05) in group K1, K2, K3, and K4 with values were 19.4692.072, 17.41.373, 20.533.411, and 23.872.528 U/ml, respectively. In contrast, MDA concentration was decreased (P0.05) with values of 492.75019.418, 492.75019.418, 457.12530.095, 454.62519.080, and 475.87537.659 ng/mL in groups K1, K2, K3, and K4, respectively. It can be concluded that the administration of MESA tend to increase SOD activity and decrease MDA concentration in this study, thus might be able to reduce oxidative stress in mice.
Co-Authors Adam Bregas Regowo Alfania Novita Putri Perdana Amilia, Annisa Amiruddin, Meilano Setiawan Andri Tilaqza Andrie Devin Susilo Hadi Anita Puspa Widiyana Ariya Indra Krisna Cahyaningrum, Asri Damayanti, Dini Sri Dewi Intan Putri Dwiyanti Elyani, Helmin Fadli, Muhammad Zainal Faizah Dwi Qurrotul Aini Fidurroty Baidho Fifin Pradina Duhitatrissari Hanny Hafiar Helmin Elyani Ike Widyaningrum Kurniasari, Laili Cinthianur Marindra Firmansyah Merlita Herbani Muhammad Yusuf Musthafa Nour Athiroh Abdoes Sjakoer Nugroho Wibisono Siti Norazizah Vina Zahirotul Izzah Yahya, Arif Yoyon Arif Martino Yudi Purnomo Yundari, Yundari