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All Journal Tarjih Tropical Livestock Journal Jurnal Veteriner
Robbaani, Muhammad
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Agriculture, Biological Sciences & Forestry Immunology & microbiology Medicine & Pharmacology Veterinary

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Comparison of Embryo Production between Simmental and Limousin Cattle in Different Seasons at Cipelang Cattle Embryo Center Robbaani, Muhammad; Nurlayasari, Irma; Khaeruddin, Khaeruddin; Wahjuningsih, Sri
Tarjih Tropical Livestock Journal Vol. 4 No. 2 (2024): Tarjih-Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v4i2.865

Abstract

Indonesia is a tropical country that has two different seasons, namely the rainy season and the dry season. Embryo production can be influenced by several factors, one of which is the season factor. Seasonal factors can affect the quality and quantity of embryos produced. The purpose of this study was to determine the comparison of embryo production of Simmental cattle with Limousin cattle in the rainy season and the dry season. The material used was 40 donor cattle divided into 20 Simmental cattle and 20 Limousin cattle aged 3 to 6 years with a body weight of 450-700 kg. The research procedure starts from superovulation using reproductive hormone injection, artificial insemination, embryo collection using flushing technique and embryo evaluation. The research method used was a case study. Data processing and analysis in this study used the independent t-test with research variables of the number of embryos collected, the quality of embryos produced, the number of corpus luteum produced, the level of response, and the level of recovery. The results of this study were no significant difference (P> 0.05) in the production of Limousin and Simmental cattle embryos in the rainy season. In contrast to the dry season, the results were (P <0.05) which means there is a difference in the production of Limousin and Simmental cattle embryos in the dry season. On the other hand, Limousin cattle showed that Limousin cattle were slightly superior to all variables in the dry season.
Dampak Dosis Etilen Glikol Terhadap Kualitas Semen Ayam Gaga dan Pengamatan Ultrastruktur Pascakriopreservasi Robbaani, Muhammad; Khaeruddin, Khaeruddin; Rachmawati, Achadiah; Yekti, Aulia Puspita Anugra; Nursita, Ita Wahju; Wahjuningsih, Sri
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Penelitian mengenai etilen glikol (EG) untuk kriopreservasi semen ayam gaga masih terbatas. Oleh karena itu, penelitian ini bertujuan untuk mengkaji efek EG sebagai krioprotektan spermatozoa selama proses pembekuan (kriopreservasi)  maupun pencairan (thawing)Penelitian ini menggunakan perlakuan yang mencakup 7% dimetil sulfoksida (DMSO) sebagai kontrol (P0), 5% EG (P1), 7% EG (P2), dan 9% EG (P3), masing-masing perlakuan dengan 10 ulangan dalam pengencer Ringer laktat kuning telur (RLKT). Evaluasi meliputi pengamatan pada sejumlah parameter antara lain, motilitas spermatozoa, viabilitas, abnormalitas, recovery rate, penurunan viabilitas pasca-pencairan, peningkatan abnormalitas dan ultrastruktur spermatozoa melalui pengamatan pada Field Emission-Scanning Electron Microscope (FE-SEM). Semen diperoleh dari tiga ayam gaga jantan berumur 10–12 bulan. Semen dikoleksi dengan metode pijat (massage). Semen yang diperoleh diencerkan dan diekuilibrasi (5°C, 2 jam),  pre-freezing (10 menit) dan disimpan dalam nitrogen (N2) cair (−196°C, 24 jam), dan dilakukan pengenceran (thawing) (37°C, 30 detik). Hasil penelitian menunjukkan bahwa EG secara signifikan memengaruhi motilitas dan viabilitas spermatozoa pascapencairan (P < 0,05), tetapi tidak memengaruhi abnormalitas (P > 0,05). Spermatozoa pada perlakuan kontrol (P0) memiliki nilai recovery rate tertinggi dan penurunan viabilitas terendah, sementara EG 7% (P2) memberikan nilai recovery rate terendah. Penurunan viabilitas spermatozoa tertinggi terjadi pada EG 9% (P3). Abnormalitas spermatozoa terendah terjadi pada perlakuan EG 5% (P1) dan tertinggi pada perlakuan EG 9% (P3). Pemeriksaan FE-SEM menunjukkan adanya kerusakan pada akrosom spermatozoa setelah pembekuan dan sebagian besar bagian tengah masih utuh.
Co-Authors Achadiah Rachmawati Aulia Puspita Anugra Yekti Ita Wahju Nursita Khaeruddin Khaeruddin Khaeruddin Khaeruddin Nurlayasari, Irma Sri Wahjuningsih