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All Journal Media Kedokteran Hewan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Sasi, Samira Musa
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Medicine & Pharmacology Veterinary Other

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The Antiproliferative and Spermicide Effects of D-Limonene on Gonadal Function in Male Albino Rats: A Comprehensive Study on Spermatogenesis and Gonadal Morphometry Herdiansyah, Akbar Dimas; Yudistira, Neo Rakka; Afandik, Nadya Ashila; Meiliza, Nazwa; Ardiansyah, Kresna Ryan; Sardjito, Trilas; Sasi, Samira Musa
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.149-162

Abstract

This study investigates the effects of D-Limonene on reproductive health parameters in albino rats, focusing on sperm quality and gonadal morphology. The study involved administering D-Limonene at various doses to assess their impacts on sperm parameters, including motility, viability, and abnormalities, as well as gonadal morphometry. The D-Limonene was applied at doses of 50, 100, and 150 mg/kg BW for 60 days, followed by histopathological analysis of testicular tissues. Results indicated that D-Limonene treatment revealed a dose-dependent decline in sperm quality parameters. Specifically, total motility, progressive motility, and viability decreased, while abnormality rates increased with higher D-Limonene doses. Notably, the highest dose (150 mg/kg BW) led to the most significant reductions in sperm motility and viability. Additionally, the presence of necrotic cells and structural changes in testicular tissues were observed, supporting the adverse impact of D-Limonene on reproductive function. The findings suggest that D-Limonene exhibits potential as an effective spermicide, highlighting its utility in population control applications. The study underscores the need for further research to fully understand the mechanisms behind these effects and their implications for reproductive health.
Kinematic Characteristics of Sperm in Bali Bulls (Bos sondaicus) Aged Over 10 Years Based on Computer-Assisted Sperm Analysis Sulistiyawati, Elis; Prastiya, Ragil Angga; Sardjito, Trilas; Saputro, Amung Logam; Maxdhameta, Styuderil Imaniro; Habib, Mohammad Nabil El; Sasi, Samira Musa; Sulistyowati, Deny; Amaliya, Anny
Jurnal Ilmu-Ilmu Peternakan Vol. 36 No. 1 (2026): April 2026
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/

Abstract

Bali cattle, developed in Indonesia, are renowned for their high parasite resistance, adaptability, and reproductive health. This study aimed to evaluate the impact of age on the kinematic parameters of sperm in Bali bulls post-thawing. Semen samples were obtained by artificial vagina methods from three Bali bulls aged between 11 and 13 years, whose age was not indicated, and one bull of 9 years, which was used as a control. The experiment was conducted in December 2023 at the Singosari National Artificial Insemination Center in Malang, East Java, for sperm parameters, including viability, motility, and morphology. Computer-assisted sperm analysis was performed. The results showed a decline in sperm quality from fresh to frozen semen. Despite this, the semen from Bali bulls met the Indonesian National Standard, demonstrating the bulls' superior reproductive capabilities. Bulls aged 11–13 years exhibited variations in sperm quality; however, these differences may reflect individual variability rather than a consistent age-related trend. Semen from bulls aged over 10 years remain suitable for use, though regular testing is essential. Further research is needed to understand these variations and ensure optimal semen quality across different ages.
Co-Authors Afandik, Nadya Ashila Amaliya, Anny Ardiansyah, Kresna Ryan Habib, Mohammad Nabil El Herdiansyah, Akbar Dimas Maxdhameta, Styuderil Imaniro Meiliza, Nazwa Prastiya, Ragil Angga Saputro, Amung Logam Sulistiyawati, Elis Sulistyowati, Deny Trilas Sardjito Yudistira, Neo Rakka