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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Medik Veteriner
Sulistyowati, Deny
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Biochemistry, Genetics & Molecular Biology Veterinary Other

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Effect of Addition of Green Tea Extract (Camellia sinensis) in Egg Yolk Tris Diluter on Spermatozoa Quality in Bali Cattle (Bos sondaicus) After Freezing Wijayanti, Ani; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Hernawati, Tatik; Sardjito, Trilas; Saputro, Amung Logam; Amaliya, Anny; Sulistyowati, Deny
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.66-74

Abstract

This study aimed to determine the effect of adding 96% ethanol extract of green tea (Camellia sinensis) in egg yolk tris diluter on the quality of spermatozoa of Bali cattle (Bos sondaicus) after freezing. The samples were divided into four different treatment groups, which contained semen sample + 13% glycerol + each egg yolk tris diluter + green tea extract (P0) 0 mg/100 ml, (P1) 0,05 mg/100 ml, ( P2) 0,10 mg/100 ml, and (P3) 0,15 mg/100 ml. The variables observed included motility, viability, and spermatozoa abnormalities. Data were analyzed using OneWay ANOVA and continued with Duncan's test (p<0,05). The results showed that the P3 group had motility of 49,00 ± 2,15, viability of 61,92 ± 1,70, and abnormality of 4,24 ± 2,16 significantly compared to the other groups. In conclusion, the P3 group with the addition of 0,15 mg/100 ml of green tea could increase the motility, viability, and abnormal spermatozoa of Bali cattle.
Optimization of BSA Column Gradient Concentrations on Post-Thaw Quality of Sexed Spermatozoa in Bali Bulls Yekti, Aulia Puspita Anugra; Setiawan, Raka; Ristrushana, Nurma Angelita; Susilawati, Trinil; Wahjuningsih , Sri; Isnaini, Nurul; Amalia, Anny; Sulistyowati, Deny; Pramudhita, Anggita Dian
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 3 (2025): December 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.03.13

Abstract

This study evaluated post-thaw semen quality and the proportions of X- and Y bearing spermatozoa in Bali cattle semen sexed by albumin of bovine serum albumin (BSA) column using two different gradients. In this study, frozen semen from a Bali bull was used, with three treatments: T0 (non-sexed frozen semen as a control), T1 (BSA 5%:8%), and T2 (BSA 5%:10%). The parameters observed were individual motility, viability, abnormality, concentration, total motile sperm (TSM), and sperm proportion.  The results showed that sperm motility in the upper fraction (UF) was highest under T2 (41.57%), whereas in the lower fraction (LF) it peaked under T1 (24.15%). Sperm viability was highest in the UF under T1 (57.69%) and in the LF under T1 (39.02%). The lowest abnormality was observed in the UF under T2 (4.64%) and in the LF under T1 (7.27%). Sperm concentration reached 30.14 million/straw in the UF under T2 and 22.27 million/straw in the LF under T2. Total sperm motility (TSM) was highest in the UF under T2 (12.57 million/straw) and in the LF under P1 (4.76 million/straw). The sperm proportions of the UF reached 75% X (T1) and the LF 81.71% Y (T1). Overall, the upper fraction obtained from the 5%:8% BSA gradient provided acceptable post-thaw sperm quality and adequate X/Y sperm enrichment, supporting its suitability for artificial insemination.   
Co-Authors Amalia, Anny Amaliya, Anny Ani Wijayanti Aulia Puspita Anugra Yekti Nurul Isnaini Pramudhita, Anggita Dian Prastiya, Ragil Angga Ristrushana, Nurma Angelita Saputro, Amung Logam Setiawan, Raka Tatik Hernawati Tri Wahyu Suprayogi Trilas Sardjito Wahjuningsih , Sri