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All Journal Jurnal Sain Veteriner Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Ovozoa: Journal of Animal Reproduction LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary

Saputro, Amung Logam

Laboratorium Klinik, Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus-C Unair Jln. Mulyorejo, Surabaya, Jawa Timur, Indonesia, 60115

Author-ID : 1219999

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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Identifikasi Escherichia coli dan Salmonella spp pada Karkas Sapi di Rumah Potong Hewan di Banyuwangi dan Resistensi Terhadap Antibiotika Faisal Fikri; Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Iwan Sahrial Hamid
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Food borne disease can be transmitted through Escherichia coli and Salmonella spp contamination. The contamination of microorganisms with high pathogenic potentials on bovine carcasses results in food borne illness. The aim of this study was to identify multidrug-resistance of Escherichia coli and Salmonella spp on carcass samples that isolated from slaughter house in Banyuwangi. Samples were collected from district of Banyuwangi, Rogojampi, Genteng and Kalibaru. This study used cross sectional study with assumption of prevalence at 50% in each contaminant, confidence level 95% and standart of error at 10%. By the number of samples should reach 96 samples. The result showed that seven samples (7,3%) were positive Escherichia coli and none samples (0%) were positive Salmonella spp. The multidrug-resistance of Escherichia coli showed that Cephalotin (42,9%), Trimethoprim (14,3%) and Erythromycin (42,9%) whereas Ampicillin, Enrofloxacin, Chloramphenicol and Tetracycline were sensitive against Escherichia coli.

Mycotoxin Binders Effect on Ovaries of Pregnant Mice Exposed to Zearalenone Ragil Angga Prastiya; Abdul Samik; Amung Logam Saputro
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Mycotoxin, a secondary metabolite produced by mushroom, has proven to cause various cytotoxic effects to animals. Zearalenone is one type of mycotoxin which is produced by Fusarium graminearum mushroom and has a cytotoxic effect to the reproduction system and cattle productivity by inducing apoptosis in the animal's ovaries, uterus, and placenta. This research tested the potentials of mycotoxin binders in eliminating the effect of zearalenone which can be seen from the expression of caspase 3, the content of malondialdehyde (MDA), and the quantity of corpus luteum in the ovaries of pregnant mice (Mus musculus). Twenty mice were grouped into five different treatments, namely K+ (not exposed to zearalenone and mycotoxin binders), K- (0.1 mg/mouse/day of zearalenone dosage), P1 (0.1 mg/mouse/day of zearalenone dosage and 0.5 mg/mouse/day of mycotoxin binder dosage), P2 (0.1 mg/mouse/day of zearalenone dosage and 1 mg/mouse/day of mycotoxin binders), and P3 (0.1 mg/mouse/day of zearalenone and 2 mg/mouse/day of mycotoxin binders), with 10-days treatment time. The results showed that the potentials of mycotoxin binders could be found on the gradual decline in the expression of caspase 3 in P1, P2 and P3 compared to that in K- (negative control). The results also showed a significant difference in the content of malondialdehyde (MDA) in K- compared to that in K+, P1, P2, and P3. Meanwhile, the quantity of corpus luteum in K- was also starkly different to that in K+, P1, P2, and P3. It can then be concluded that mycotoxin binders are very effective in eliminating the exposure of zearalenone.

Aluminosilikat Berpotensi Menekan Gangguan Reproduksi Mikotoksin Zearalenon Berdasarkan Pengamatan Jumlah Folikel dan Ekspresi Caspase-9 Ovarium Muhammad Thohawi Elziyad Purnama; Imam Mustofa; Tri Wahyu Suprayogi; Abdul Samik; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Zearalenone is a resorcylic acid lactone produced by fungal Fusarium graminearum in contaminated edible grains and can cause reproduction disorder in animals by binding to estrogen receptors on target cells. The aim of this study was to assess the potential use of aluminosilicates as mycotoxin binders to eliminate the adverse effect of zearalenone by examining the number of follicles and caspase-9 expression in the ovary of mice. The study adopted a completely randomized simple design using 20 mices which were randomly divided into five group each of which consisted of four mices. Five treatment groups consisted of K+ (without zearalenone and aluminosilicates); K- (treated with zearalenone 0.1 mg/mice/day); P1 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 0.5 mg/mice/day); P2 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 1 mg/mice/day); and P3 were treated with zearalenone 0,1 mg/mice/day and aluminosilicates 2 mg/mice/day) with gastric tube daily for 10 days. The data obtained from this study were analyzed by analysis of variance and proceeded with Duncan test. The result showed that the primary follicles, secondary follicles, tertiary follicles and de Graaf follicles increased significantly on P3 treatment group. Caspase-9 expressions decreased significantly in all of aluminosilicates groups as compared to positive control. The treatment of mice with zearalenone and aluminosilicates increases the number of follicles and decreased caspase-9 expression in the ovary of mice. ABSTRAK Zearalenon merupakan senyawa resorcylic acid lactone yang diproduksi oleh jamur Fusarium graminearum dan dapat mengakibatkan gangguan reproduksi pada ternak dengan membentuk ikatan pada reseptor estrogen. Penelitian ini bertujuan untuk menguji potensi aluminosilikat terhadap mencit yang telah dipapar zearalenon pada aspek jumlah folikel dan ekspresi caspase-9 organ ovarium. Penelitian ini menggunakan 20 ekor mencit yang dibagi menjadi lima kelompok perlakuan dengan masing-masing empat ulangan, yakni K+ tanpa dipapar zearalenon dan aluminosilikat; K- dosis zearalenon 0,1 mg/ekor/hari; P1 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 0,5 mg/ekor/hari; P2 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 1 mg/ekor/hari; P3 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 2 mg/ekor/hari dengan sonde lambung selama sepuluh hari. Data hasil skoring dan perhitungan dianalisis dengan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa jumlah folikel primer, sekunder, tersier dan de Graaf pada P3 terjadi peningkatan yang signifikan. Ekspresi caspase-9 ovarium menunjukkan penurunan pada semua perlakuan aluminosilikat. Simpulan penelitian ini adalah mencit yang dipapar zearalenon dan kemudian diberi aluminosilikat mengalami peningkatan jumlah folikel dan penurunan ekspresi caspase-9 pada organ ovarium.

Potensi Toksin Binders untuk Mengurangi Efek Mikotoksin Zearalenon terhadap Panjang, Bobot, dan Jumlah Fetus Mencit (TOXIN BINDERS HAS THE POTENTIAL TO DECREASE ZEARALENONE MYCOTOXIN ON FETAL LENGTH, WEIGHT AND LITTER SIZE OF PREGNANT MICE) Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Ragil Angga Prastiya
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.471 KB)

Corn is a source of feed with protein content to increase livestock production. Zearalenone is a mycotoxin produced by Fusarium graminearum obligate in corn. The mycotoxins allegedly can cause pregnancy disorders to fetal malformations. Using toxin binders to inhibit zearalenone mycotoxins. The purpose of this study was to determine the potential of toxin binders in reducing the effect of zearalenon on the length, weight and fetal litter size of pregnant mice (Mus musculus). This study used 20 balb/c mice divided into control group (C-) without toxin binders and zearalenone; (C+) was given only zearalenone 0.1 mg / mice / day; and treatment groups T1; T2; T3 was given zearalenone 0.1 mg / mice / day and toxin binders 0.5; 1; 2 mg / mice / day. The toxin binders and zearalenone was done with orally for 14 days. The data were analyzed using Analysis of variance test and continued by Duncan test. The results showed that C-, C+, T1 were significantly different (p<0.05) with T2 and T3 on the fetal length. The fetal weight showed C-, T1, T2, T3 was significantly different (p<0.05) with C+. The result of fetal litter size showed that were not significantly different (p>0.05) between treatment groups. It was concluded that toxin binders could decrease the effect of zearalenon thus increasing the length and weight of the fetus but not affecting the fetal litter size of pregnant mice (M. musculus).

Ekstrak Etanol Kulit Buah Naga Menurunkan Indikasi Neoplasia Mammae Tikus Putih Berdasarkan Histopatologi dan Inhibitor Siklooksigenase-2 Muhammad Thohawi Elziyad Purnama; Ragil Angga Prastiya; Faisal Fikri; Amung Logam Saputro; Bodhi Agustono
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Cancer caused uncontrolled cell proliferation and triggered by changes on cell information that regulate growth function. Each cell has different potential so many cancer prevalence such as endometrial cancer, lymph node cancer, lung cancer, skin and mammary gland cancer. The aims of this study were to assess the potential of dragon fruit skin ethanolic extract on white rats (Rattus norvegicus) exposed 7,12- Dimethylbenz(á)antrasena (DMBA) on mammary gland based on histopathological features and cyclooxygenase-2 (Cox-2) intensity. This study were used 20 of rats randomly divided into five group and each groups consisted of four rats, i.e: K+ weren’t treated DMBA and extract; K- were treated with DMBA; P1 were treated with DMBA and extract 10 mg/kg BW; P2 were treated with DMBA and extract 15 mg/kg BW; P3 were treated with DMBA and extract 20 mg/kg BW. The DMBA was given by intra dermal injection during twice a week for five weeks and the extracts with gastric tube everyday till 14 days. The data was analyzed by Anova test and continued with Duncan test. The result showed that the histopathological features were decrease significantly on P3. The variables of Cox-2 intensity were decrease significantly on P1, P2 and P3. Conclusion of this study was ethanol extract of dragon fruit skin can decrease neoplastic indication of mammary gland on white rats (R. norvegicus) based on histopathological features and Cox-2 inhibitors.

The Potentials of Robusta Coffee Seed Extract as Antioxidant on Kidney Histopathology in Mice Exposed to Monosodium Glutamate: Potensi Pemberian Ekstrak Biji Kopi Robusta sebagai Antioksidan Terhadap Gambaran Histopatologi Ginjal Mencit yang Dipapar Monosodium Glutamat Wisudawati, Feby Dwi; Hidajati, Nove; Fikri, Faisal; Santoso, Kuncoro Puguh; Saputro, Amung Logam; Purnama, Muhammad Thohawi Elziyad
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.3

The purpose of this study was to determine the potential of administration robusta coffee extract as an antioxidant on the renal histopathology in mice exposed to monosodium glutamate (MSG) orally. This study used 20 adult male rats with Balb C strain divided into 5 experimental groups, namely K + (MSG 0.12 mg / head and Vitamin C 6 mg/mice), K- (MSG 0.12 mg/mice and Sodium carboxymethyl cellulose (CMC Na) 0,1 ml/mice), P1 (MSG 0.12 mg/mice and Robusta coffee extract 0.1 mg/mice), P2 (MSG 0.12 mg/mice and Robusta coffee extract 0.2 mg/mice), and P3 (MSG 0.12 mg/mice and 0.4 mg Robusta coffee extract mg/mice). The experimental group was treated every day at intervals between MSG and Vitamin C, CMC Na, and Robusta coffee extract for 60 min, the treatment was carried out for 42 days. Kidney histopathology scores were analyzed using the Saphiro-Wilk and Levene test to evaluate homogeneity, followed by the Kruskal Wallis test. The results of the Saphiro Wilk and Levene statistical analysis obtained p<0.05, thus the data continued with the Kruskal Wallis test scores of kidney cell damage obtained 0.043 which showed that the data were significant. The mean score of kidney cell damage in the treatment groups was K+ 1.60; K- 1.63; P1 1.70; P2 1.63; and P3 1.73. The conclusion is that Robusta coffee bean extract did not show a decrease in kidney cell damage that was different between treatment groups. Further research is needed to find out about the effect of different dosages of Robusta Coffee Extract to different organs

Hidroponik Fodder Jagung sebagai Substitusi Hijauan Pakan Ternak Ditinjau dari Produktivitas Susu Kambing Sapera Saputro, Amung Logam; Hamid, Iwan Sahrial; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Hidroponik adalah sistem bercocok tanam menggunakan media cair dan nutrisi-nutrisi yang diperlukan untuk pertumbuhan tanaman. Keuntungan bercocok tanam dengan hidroponik yakni tanaman tidak merusak tanah, tidak membutuhkan tempat yang luas, tanpa pestisida atau obat hama yang merusak tanah, mempercepat pertumbuhan dan menjaga kualitas tanaman. Penelitian ini menggunakan perbandingan konsentrat dan fodder jagung yakni P1 (100:0), P2 (60:40), P3 (75:25) dan P4 (0:100). Hasil penelitian menunjukan bahwa kuantitas susu pada hari ke-1, ke-2 dan ke-3 menunjukkan P4 berbeda nyata (p<0,05) dengan P1, P2, dan P3. Hal ini menunjukkan penurunan kuantitas susu pada P4 dan kuantitas susu terbanyak pada P1 dan P3. Simpulan penelitian perlakuan pemberian fodder jagung 25% dan konsentrat 75% serta perlakuan konsentrat 100% dapat meningkatkan kuantitas susu kambing Sapera.

Kualitas Semen Segar Sapi Rambon Banyuwangi Dalam Pengencer Tris Kuning Telur Dan Susu Skim Kuning Telur Safitri, Ainun Merta; Sardjito, Trilas; Wibawati, Prima Ayu; Mustofa, Imam; Saputro, Amung Logam; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui kualitas semen sapi Rambon Banyuwangi dengan menggunakan dua pengencer yang berbeda, yakni tris kuning telur dan susu skim kuning telur. Metode penelitian ini menggunakan rancangan acak lengkap (RAL). Penelitian ini menggunakan satu ekor pejantan sapi Rambon untuk penampungan semen. Semen ditampung sebanyak sembilan kali untuk dua perlakuan pengencer yaitu P1: semen+tris kuning telur dan P2: semen+susu skim kuning telur. Parameter yang diukur pada penelitian ini adalah motilitas, viabilitas dan abnormalitas spermatozoa. Setiap parameter pada masing-masing perlakuan dibandingkan dengan menggunakan uji T dua sampel. Hasil penelitian ini menunjukkan bahwa P1 lebih mampu mempertahankan motilitas dan viabilitas spermatozoa sapi Rambon Banyuwangi dibanding dengan P2, dimana motilitas P1 yaitu 67,22±4,41% dengan kecepatan 3 dan motilitas P2 yaitu 59,44±8,46% dengan kecepatan 2 berbdea nyata (p<0,05). Viabilitas P1 adalah 77,44±3,21% dan P2 yaitu 73,56±3,32% berbeda nyata (p<0,05). Tidak terdapat perbedaan yang nyata (p>0,05) terhadap abnormalitas spermatozoa baik primer maupun sekunder sapi Rambon Banyuwangi pada kedua pengencer dimana nilai abnormalitas primer P1 yaitu 0,33±0,50% dan P2 0,22±0,44%, abnormalitas sekunder P1 yaitu 8,33±2,17% dan P2 7,56±2,24%. Disimpulkan bahwa media pengencer tris kuing telur dan susu skim kuning telur layak untuk digunakan IB dengan semen segar.

Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing Masyitoh, Herlina; Suprayogi, Tri Wahyu; Praja, Ratih Novita; Srianto, Pudji; Madyawati, Sri Pantja; Saputro, Amung Logam
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui perbedaan persentase motilitas dan viabilitas semen kambing Sapera terhadap evaluasi semen segar dan before freezing dengan penambahan pengencer tris kuning telur dan susu skim kuning telur. Metode penelitian ini adalah pengambilan semen, evaluasi semen segar (pemeriksaan makroskopis dan mikroskopis), pengenceran semen, gliserolisasi, ekuilibrasi dan evaluasi before freezing. Penelitian ini terdiri dari P0, P1 dan P2, masing-masing perlakuan terdiri dari sembilan ulangan. P0 adalah semen segar kambing Sapera, P1 adalah semen kambing Sapera+pengencer tris kuning telur dan P2 adalah semen kambing Sapera+pengencer susu skim kuning telur. Data yang diperoleh dengan Uji T2 sampel dependent, membandingkan P0 dan P1 serta P0 dan P2. Data menunjukkan perbedaan sangat nyata (p<0,01) pada perlakuan P0 dan P1 persentase motilitas 76,11%; 65,56% dan viabilitas 82,33%;72,22%. P0 dan P2 persentase motilitas 76,11%;61,11% dan viabilitas 82,33%;67,78%. Hasil tersebut menunjukkan bahwa penelitian persentase motilitas dan viabilitas spermatozoa kambing Sapera mengalami penurunan dari semen segar pada tris kuning telur dan susu skim kuning telur before freezing.

Analisis Kelayakan Usaha Secara Finansial dan Efisiensi Produksi di Peternakan Sapi Perah PT. Fructi Agri Sejati Kabupaten Jombang Khafsah, Khafsah; Warsito, Sunaryo Hadi; Prastiya, Ragil Angga; Sardjito, Trilas; Saputro, Amung Logam; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui tentang analisis kelayakan usaha secara finansial dan efisiensi produksi peternakan sapi perah di PT. Fructi Agri Sejati Kabupaten Jombang yang mempunyai 100 ekor sapi Peranakan Fries Holland (PFH). Jenis penelitian deskriptif yang dilaksanakan di Dusun Komboh Desa Sambirejo Kecamatan Wonosalam Kabupaten Jombang. Penelitian menggunakan metode survey yang kemudian diperoleh data primer dan sekunder dari PT. Fructi Agri Sejati melalui wawancara dengan menggunakan kuesioner. Analisis kelayakan usaha berdasarkan kriteria kelayakan investasi meliputi Net Present Value (NPV), Net Benefit Cost Ratio (Net B/C), Internal Rate Return (IRR), Payback Periode (PP) dan R/C Ratio untuk analisis efisiensi produksi. Hasil penelitian menunjukkan besar pendapatan rata-rata per tahun sebesar Rp.141.669.425, dengan rata-rata penerimaan sebesar Rp.898.382.687 yang bersumber dari hasil penjualan susu, pedet jantan, sapi afkir, pejantan, sapi laktasi dan pupuk kandang. Berdasarkan hasil penelitian usaha layak untuk dijalankan karena semua kriteria investasi mampu dicapai, memiliki NPV>0 yaitu Rp.181.016.633, Net B/C>0 yaitu 1,15, IRR sebesar 12,3% lebih besar dari tingkat diskonto yang digunakan dan PP selama 7,2 tahun. Efisiensi produksi dinyatakan sudah efisien karena nilai R/C>0 yaitu 1,18.

Co-Authors Abdul Samik Agustono, Bodhi Amaliya, Anny Anastasya, Anastasya Ani Wijayanti Ashari, Lukman Ashilla Afandik, Nadya Atikah Madarina Hisyam, Mirza Boedi Setiawan Cahya, Rizka Wulan Dini, Sayyida Kamila Dinul, Ahmad Rajul Erma Safitri ERMA SAFITRI Estoepangestie, Agnes Theresia Soelih Faisal Fikri Faizah, Ayu Nur Farhan, Muhammad Riesta Haditanojo, Wiyanto Hamid, Iwan Sharial Hani Plumeriastuti Hermin Ratnani Hidajati, Nove Hidayatuh Sholihah, Alfi IMAM MUSTOFA Imam Mustofa iqbal dwi warsito Ira Sari Yudaniayanti Ivanalee, Angela Swasti Iwan Sahrial Hamid Iwan Sahrial Hamid Khafsah, Khafsah Lailiyah, Fahlevi Lake, Werenfridus Kono Lestari, Dewi Sri Luqman, Epy Muhammad Mafruchati, Maslichah Masyitoh, Herlina Milladiya Khuriyah Zahra, Reyza Mirni Lamid Muhammad Gufron Muhammad Thohawi Elziyad Purnama Muhandis, Putri Jauza Aulia Mumtazi, Fauzan Murjianingsih, Fitriani Nurina Naqsmagita, Salsabilla Nusdianto Triakoso Pahlevy, Jolly Rifqi Pasha, Zehansyah Algifari Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Primarizky, Hardany Pudji Srianto Puspitasari, Vina Putri, Lusi Rosita Sartono Rachmanita, Rona Taufiqul Rahmadhani, Nurmitasari Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Safitri, Ainun Merta Santoso, Kuncoro Puguh Sarudji, Suryanie Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setyawan, Firman Solikhah, Tridiganita Intan Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulaeman, Nur Sa'adah Sulistyowati, Deny Sunarso, Agus Tatik Hernawati Tito, Muhammad Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Trisnawati, Sharfina Habibah Tyasningsih, Wiwiek Ulinuha, Meidina Zulva Umama, Ayu Riza Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widayani, Prasita Widya Paramita Lokapirnasari Wisudawati, Feby Dwi Yudhana, Aditya Yulianto, Rangga Yunita, Maya Nurwartanti Yustinasari, Lita Rakhma

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