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Mycotoxin Binders Effect on Ovaries of Pregnant Mice Exposed to Zearalenone Ragil Angga Prastiya; Abdul Samik; Amung Logam Saputro
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Mycotoxin, a secondary metabolite produced by mushroom, has proven to cause various cytotoxic effects to animals. Zearalenone is one type of mycotoxin which is produced by Fusarium graminearum mushroom and has a cytotoxic effect to the reproduction system and cattle productivity by inducing apoptosis in the animal's ovaries, uterus, and placenta. This research tested the potentials of mycotoxin binders in eliminating the effect of zearalenone which can be seen from the expression of caspase 3, the content of malondialdehyde (MDA), and the quantity of corpus luteum in the ovaries of pregnant mice (Mus musculus). Twenty mice were grouped into five different treatments, namely K+ (not exposed to zearalenone and mycotoxin binders), K- (0.1 mg/mouse/day of zearalenone dosage), P1 (0.1 mg/mouse/day of zearalenone dosage and 0.5 mg/mouse/day of mycotoxin binder dosage), P2 (0.1 mg/mouse/day of zearalenone dosage and 1 mg/mouse/day of mycotoxin binders), and P3 (0.1 mg/mouse/day of zearalenone and 2 mg/mouse/day of mycotoxin binders), with 10-days treatment time. The results showed that the potentials of mycotoxin binders could be found on the gradual decline in the expression of caspase 3 in P1, P2 and P3 compared to that in K- (negative control). The results also showed a significant difference in the content of malondialdehyde (MDA) in K- compared to that in K+, P1, P2, and P3. Meanwhile, the quantity of corpus luteum in K- was also starkly different to that in K+, P1, P2, and P3. It can then be concluded that mycotoxin binders are very effective in eliminating the exposure of zearalenone.

Faktor-Faktor Risiko Kawin Berulang pada Sapi Potong di Kecamatan Licin, Kabupaten Banyuwangi, Jawa Timur Putri Nurmalia Damayanti; Trilas Sardjito; Ragil Angga Prastiya
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Nowadays, beef cattle farms in Licin Subdistrict, Banyuwangi Regency have problems in providing beef demand because the less of productivity. This problems occured due to the repeat breeding case and decrease the efficiency and productivity of livestock. It suspected because of the lack of hygine and sanitation from farmers and inseminator. This study aimed to correlate the factors and cases of repeat breeding on beef cattle in Licin Subdistrict, Banyuwangi Regency. It was a descriptive study which used 69 cow withsimple random sampling method. Primary data was obtained from direct observation of livestock, then continued with interviewing farmers using in-depth study system. While secondary data is obtained from the records of inseminator. It included the term of farming, farmer’s ability to detect estrus cycle, housingand the feeding of beef cattle. The collected data was analyzed by descriptive statistic, then processed by using SPSS 22.0. Chi Square Test (X2) was used to determine the relation between the causal factors and repeat breeding. The results of this study indicate the prevalence of repeat breeding in Licin Subdistrict, Banyuwangi Regency by 64%. All the factors such as the term of farming, farmer’s ability to detect estrus cycle, and feeding of beef cattle are correlated with the incidence of repeat breeding.

Aluminosilikat Berpotensi Menekan Gangguan Reproduksi Mikotoksin Zearalenon Berdasarkan Pengamatan Jumlah Folikel dan Ekspresi Caspase-9 Ovarium Muhammad Thohawi Elziyad Purnama; Imam Mustofa; Tri Wahyu Suprayogi; Abdul Samik; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Zearalenone is a resorcylic acid lactone produced by fungal Fusarium graminearum in contaminated edible grains and can cause reproduction disorder in animals by binding to estrogen receptors on target cells. The aim of this study was to assess the potential use of aluminosilicates as mycotoxin binders to eliminate the adverse effect of zearalenone by examining the number of follicles and caspase-9 expression in the ovary of mice. The study adopted a completely randomized simple design using 20 mices which were randomly divided into five group each of which consisted of four mices. Five treatment groups consisted of K+ (without zearalenone and aluminosilicates); K- (treated with zearalenone 0.1 mg/mice/day); P1 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 0.5 mg/mice/day); P2 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 1 mg/mice/day); and P3 were treated with zearalenone 0,1 mg/mice/day and aluminosilicates 2 mg/mice/day) with gastric tube daily for 10 days. The data obtained from this study were analyzed by analysis of variance and proceeded with Duncan test. The result showed that the primary follicles, secondary follicles, tertiary follicles and de Graaf follicles increased significantly on P3 treatment group. Caspase-9 expressions decreased significantly in all of aluminosilicates groups as compared to positive control. The treatment of mice with zearalenone and aluminosilicates increases the number of follicles and decreased caspase-9 expression in the ovary of mice. ABSTRAK Zearalenon merupakan senyawa resorcylic acid lactone yang diproduksi oleh jamur Fusarium graminearum dan dapat mengakibatkan gangguan reproduksi pada ternak dengan membentuk ikatan pada reseptor estrogen. Penelitian ini bertujuan untuk menguji potensi aluminosilikat terhadap mencit yang telah dipapar zearalenon pada aspek jumlah folikel dan ekspresi caspase-9 organ ovarium. Penelitian ini menggunakan 20 ekor mencit yang dibagi menjadi lima kelompok perlakuan dengan masing-masing empat ulangan, yakni K+ tanpa dipapar zearalenon dan aluminosilikat; K- dosis zearalenon 0,1 mg/ekor/hari; P1 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 0,5 mg/ekor/hari; P2 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 1 mg/ekor/hari; P3 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 2 mg/ekor/hari dengan sonde lambung selama sepuluh hari. Data hasil skoring dan perhitungan dianalisis dengan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa jumlah folikel primer, sekunder, tersier dan de Graaf pada P3 terjadi peningkatan yang signifikan. Ekspresi caspase-9 ovarium menunjukkan penurunan pada semua perlakuan aluminosilikat. Simpulan penelitian ini adalah mencit yang dipapar zearalenon dan kemudian diberi aluminosilikat mengalami peningkatan jumlah folikel dan penurunan ekspresi caspase-9 pada organ ovarium.

Potensi Toksin Binders untuk Mengurangi Efek Mikotoksin Zearalenon terhadap Panjang, Bobot, dan Jumlah Fetus Mencit (TOXIN BINDERS HAS THE POTENTIAL TO DECREASE ZEARALENONE MYCOTOXIN ON FETAL LENGTH, WEIGHT AND LITTER SIZE OF PREGNANT MICE) Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Ragil Angga Prastiya
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.471 KB)

Corn is a source of feed with protein content to increase livestock production. Zearalenone is a mycotoxin produced by Fusarium graminearum obligate in corn. The mycotoxins allegedly can cause pregnancy disorders to fetal malformations. Using toxin binders to inhibit zearalenone mycotoxins. The purpose of this study was to determine the potential of toxin binders in reducing the effect of zearalenon on the length, weight and fetal litter size of pregnant mice (Mus musculus). This study used 20 balb/c mice divided into control group (C-) without toxin binders and zearalenone; (C+) was given only zearalenone 0.1 mg / mice / day; and treatment groups T1; T2; T3 was given zearalenone 0.1 mg / mice / day and toxin binders 0.5; 1; 2 mg / mice / day. The toxin binders and zearalenone was done with orally for 14 days. The data were analyzed using Analysis of variance test and continued by Duncan test. The results showed that C-, C+, T1 were significantly different (p<0.05) with T2 and T3 on the fetal length. The fetal weight showed C-, T1, T2, T3 was significantly different (p<0.05) with C+. The result of fetal litter size showed that were not significantly different (p>0.05) between treatment groups. It was concluded that toxin binders could decrease the effect of zearalenon thus increasing the length and weight of the fetus but not affecting the fetal litter size of pregnant mice (M. musculus).

Aktivitas Antibakteri Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Pertumbuhan Bakteri Pseudomonas aeruginosa Secara In Vitro (ANTIBACTERIAL ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAF EXTRACT ON GROWTH OF BACTERIA PSEUDOMONAS AERUGINOSA IN VITRO) Faisal Fikri; Imas Hapsari Rahmaningtyas; Ragil Angga Prastiya; Muhammad Thohawi Elziyad Purnama
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to measure antibacterial activity of soursop leaf extract (Annona muricata L.) on bacterial growth inhibition of Pseudomonas aeruginosa by diffuse disc method. The result of the inhibition zone was analyzed using Analysis of Variance and followed using Duncan test, to show highly significantly different (p<0.01). The inhibition number of negative control (C-) was 0.00e±0.00 and the positive control (C+) was 31.31a±0.57. The result of concentration 1 (T1) was 14.49b±4.96; concentration 2 (T2) 8.98c±1.29; concentration 3 (T3) 5.84cd±1.85; concentration 4 (T4) 5.56cd±1.58; concentration 5 (T5) 2.85de±0.28; concentration 6 (T6) 2.98de±0.53; concentration 7 (T7) 2.82de±1.59; concentration 8 (T8) 2.41de±1.10; concentration 9 (T9) 2.20de±0.34; concentration 10 (T10) 1.10e±0.19; and concentration 11 (T11) 0.00e±0.00. The increase of soursop leaf extract concentration showed high inhibition diameter of bacterial growth. It concluded that soursop leaf extract have an antibacterial activity to inhibit P. aeruginosa growth with Minimal Inhibitory Concentration (MIC) 125 ppm.

Ekstrak Etanol Kulit Buah Naga Menurunkan Indikasi Neoplasia Mammae Tikus Putih Berdasarkan Histopatologi dan Inhibitor Siklooksigenase-2 Muhammad Thohawi Elziyad Purnama; Ragil Angga Prastiya; Faisal Fikri; Amung Logam Saputro; Bodhi Agustono
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Cancer caused uncontrolled cell proliferation and triggered by changes on cell information that regulate growth function. Each cell has different potential so many cancer prevalence such as endometrial cancer, lymph node cancer, lung cancer, skin and mammary gland cancer. The aims of this study were to assess the potential of dragon fruit skin ethanolic extract on white rats (Rattus norvegicus) exposed 7,12- Dimethylbenz(á)antrasena (DMBA) on mammary gland based on histopathological features and cyclooxygenase-2 (Cox-2) intensity. This study were used 20 of rats randomly divided into five group and each groups consisted of four rats, i.e: K+ weren’t treated DMBA and extract; K- were treated with DMBA; P1 were treated with DMBA and extract 10 mg/kg BW; P2 were treated with DMBA and extract 15 mg/kg BW; P3 were treated with DMBA and extract 20 mg/kg BW. The DMBA was given by intra dermal injection during twice a week for five weeks and the extracts with gastric tube everyday till 14 days. The data was analyzed by Anova test and continued with Duncan test. The result showed that the histopathological features were decrease significantly on P3. The variables of Cox-2 intensity were decrease significantly on P1, P2 and P3. Conclusion of this study was ethanol extract of dragon fruit skin can decrease neoplastic indication of mammary gland on white rats (R. norvegicus) based on histopathological features and Cox-2 inhibitors.

Effect of Cirripedia sp. Flour As Subtitutional Feed Source of Protein Against Dried Disease and Organic Material Rabbit Rain Type Rex Bodhi Agustono; Widya Paramita Lokapirnasari; Mohammad Anam Al Arif; Ragil Angga Prastiya; Faisal Fikri
Bantara Journal of Animal Science Vol 2, No 1 (2020)
Publisher : Program Studi Peternakan, Fakultas Pertanian, Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v2i1.636

The aim of this research was to determine the effect of Cirripedia sp. flour as feed substitution of protein source in feed on the digestibility of dry matter and organic matter in 100 male rex broilers. The research was experimental by using a completely randomized design with four treatments and five replications. Substitution of Cirripedia sp. flour respectively in feed were 0%, 2.5%, 5%, 7.5% given for four weeks. Data collection was carried out in the fourth week (for seven days) which included consumption data, manure weight and analysis of treatment feed and manure analysis. The data were analyzed using Analysis of Variance and the difference between treatments was determined by the Duncan test. The results showed that there was no significant difference (p>0.05) and that there was no significant difference (p>0.05) on the digestibility of rex broilers. This shows that the substitution of Cirripedia sp. flour in feed can replace animal protein sources in fish meal in complete feed rabbits.Key words : Cirripedia sp, digestibility, feed subtitution, flour, rabbit.

Pengaruh Subtitusi Tepung Ikan Dengan Tepung Bernacle Terhadap Berat Organ Pencernaan dan Lemak Abdominal Kelinci Pedaging Jantan (Orytolagus cuniculus) Bodhi Agustono; Maya Nurwartanti Yunita; Sunaryo Hadi Warsito; Widya Paramita Lokapirnasari; Mohammad Anam Al Arif; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Nutrisi Ternak Tropis Vol 3, No 1 (2020): JNT | Jurnal Nutrisi Ternak Tropis Maret 2020
Publisher : Jurnal Nutrisi Ternak Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2020.003.01.8

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung teritip (Cirripedia sp.) Sebagai pengganti pakan dari tepung ikan pada pakan kelinci, seperti yang diamati dari lemak abdominal dan berat organ pencernaan kelinci jantan rex. Kelinci sebanyak 100 ekor ditempatkan pada kandang individu dengan pakan standart yang sesuai kebutuhan mereka secara ad libitum. Persentase feed substitusi dengan tepung teritip dengan empat perlakuan dan lima replikasi dalam pakan P0 (TI 15% dan TT 0%), P1 (TI 12,5% dan TT 2,5%), P2 (TI 10% dan TT 5%), P3 (TI 7,5% dan TT 7,5%). Perlakuan dilakukan selama 4 minggu, data dikumpulkan setelah pengorbanan akhir perlakuan. Usus kecil kelinci dikumpulkan dan menimbang organ pencernaan termasuk lambung, usus kecil dan usus besar. Hasil penelitian dianalisis menggunakan analisis varians dengan tingkat signifikansi 0,05. Berdasarkan hasil analisis menunjukkan bahwa tidak ada perbedaan yang signifikan (p> 0,05) pada lemak perut. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan (p> 0,05) dalam berat organ pencernaan (lambung, usus kecil, dan usus besar). Tepung teritip dapat digunakan sebagai pakan pengganti tepung ikan dalam pakan lengkap untuk kelinci jenis rex hingga 7,5%.

MORFOLOGI DAN MORFOMETRI SPERMATOZOA MUSANG LUWAK (Paradoxurus hermaphroditus) Ragil Angga Prastiya; Diana Santi; Ade Miftakhul Aziz; Hanun Putri Nuraida
ZOO INDONESIA Vol 31, No 2 (2022): Desember 2022
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v31i2.4307

Analisis morfologi dan morfometri spermatozoa musang dengan metode pewarnaan khusus Eosin-Nigrosin dapat digunakan untuk mengukur kualitas kesehatan reproduksi musang jantan melalui hasil pengamatan abnormalitas morfologi spermatozoa dan nilai morfometri, serta dapat membantu dalam upaya peningkatan populasi musang. Musang (Paradoxurus hermaphroditus) adalah mamalia liar yang diklasifikasikan ke dalam keluarga Viverridae dan termasuk dalam genus Paradoxurus. Musang dikategorikan least concern berdasar International Union for Conservation of Nature (IUCN). Salah satu cara untuk melestarikan musang adalah dengan mempelajari struktur dan fungsi dari organ reproduksi musang jantan, salah satunya melalui pengamatan morfologi dan morfometri spermatozoa. Penelitian ini menggunakan 8 ekor musang jantan yang berusia 1-3 tahun dan dewasa kelamin. Sebanyak 100 sampel spermatozoa diamati kemudian diukur panjang kepala, lebar kepala, perimeter atau lingkar kepala, luas area, ellipticity, elongation, roughness dan regularity nya. Parameter morfologi dijelaskan secara deskriptif dengan melihat morfologi normal dan abnormal dari spermatozoa musang. Hasil penelitian ini menunjukkan bahwa spermatozoa musang memiliki panjang kepala 9,67±0,60 μm, lebar kepala 4,73±0,37 μm, luas area 40,51± 4,60 μm2, perimeter atau lingkar spermatozoa 22,64±1,24 μm, ellipticity 2.05±0,017 μm, elongation 0.34±0.04 μm, roughness 0.99±0.07 μm, regularity 0.89±0.07 μm, panjang ekor 62.01±1.76 μm, dan panjang total spermatozoa 71,68±1,89 μm. Dengan demikian dapat disimpulkan bahwa pengamatan morfometri spermatozoa pada musang dapat digunakan sebagai salah satu parameter uji fertilitas musang jantan.

MORFOLOGI DAN MORFOMETRI SPERMATOZOA MUSANG LUWAK (Paradoxurus hermaphroditus) Ragil Angga Prastiya; Diana Santi; Ade Miftakhul Aziz; Hanun Putri Nuraida
ZOO INDONESIA Vol 31, No 2 (2022): Desember 2022
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v31i2.4307

Analisis morfologi dan morfometri spermatozoa musang dengan metode pewarnaan khusus Eosin-Nigrosin dapat digunakan untuk mengukur kualitas kesehatan reproduksi musang jantan melalui hasil pengamatan abnormalitas morfologi spermatozoa dan nilai morfometri, serta dapat membantu dalam upaya peningkatan populasi musang. Musang (Paradoxurus hermaphroditus) adalah mamalia liar yang diklasifikasikan ke dalam keluarga Viverridae dan termasuk dalam genus Paradoxurus. Musang dikategorikan least concern berdasar International Union for Conservation of Nature (IUCN). Salah satu cara untuk melestarikan musang adalah dengan mempelajari struktur dan fungsi dari organ reproduksi musang jantan, salah satunya melalui pengamatan morfologi dan morfometri spermatozoa. Penelitian ini menggunakan 8 ekor musang jantan yang berusia 1-3 tahun dan dewasa kelamin. Sebanyak 100 sampel spermatozoa diamati kemudian diukur panjang kepala, lebar kepala, perimeter atau lingkar kepala, luas area, ellipticity, elongation, roughness dan regularity nya. Parameter morfologi dijelaskan secara deskriptif dengan melihat morfologi normal dan abnormal dari spermatozoa musang. Hasil penelitian ini menunjukkan bahwa spermatozoa musang memiliki panjang kepala 9,67±0,60 μm, lebar kepala 4,73±0,37 μm, luas area 40,51± 4,60 μm2, perimeter atau lingkar spermatozoa 22,64±1,24 μm, ellipticity 2.05±0,017 μm, elongation 0.34±0.04 μm, roughness 0.99±0.07 μm, regularity 0.89±0.07 μm, panjang ekor 62.01±1.76 μm, dan panjang total spermatozoa 71,68±1,89 μm. Dengan demikian dapat disimpulkan bahwa pengamatan morfometri spermatozoa pada musang dapat digunakan sebagai salah satu parameter uji fertilitas musang jantan.

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