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All Journal Jurnal Biodjati Journal of Community Service
Nelis Hernahadini
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Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Industrial & Manufacturing Engineering Social Sciences

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Weissella paramesenteroides: A Lactic Acid Bacteria Producing Glutathione from Fermented Vegetable Based Keciwis Leaf Luthfia Hastiani Muharram; Haryanto; Wulan Pertiwi; Nelis Hernahadini; Ima Mukaromah
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.30589

Abstract

Glutathione (GSH), is one of the essential antioxidants to prevent free radicals and support the immune system. The low level of glutathione is associated with some diseases such as cancer, Alzheimer's, Parkinson's, and AIDS. Lactic acid bacteria (LAB) can produce glutathione. Indonesia, as a mega biodiversity country, has various vegetables and LAB sources that remain underexplored. This research aims to explore LAB-producing glutathione from fermented products derived from Indonesia's typical plant, the keciwis leaf. The method of this research includes sample preparation, lactic acid bacteria isolation, secondary metabolite production with cysteine precursor addition, glutathione analysis by Ellman's assay, identification of 16S rRNA gene of lactic acid bacteria, and glutathione qualitative analysis by HPLC.  Fermented keciwis leaf-based products contain lactic acid bacteria with positive gram characteristics, can produce 0.33 – 0.37 mM extracellular glutathione. Precursor cysteine addition significantly increases glutathione (p<0,05). The selective lactic acid bacteria producing glutathione was identified as belonging to the genus Weissella, with a similarity value of 98.50%, closely related to Weissella paramesenteroides. Extracellular glutathione produced by this culture showed qualitative results on HPLC with a retention time of 6.34 minutes, indicating the presence of the sulfihydryl group. This study identified a new Indonesia source of lactic acid bacteria of Indonesian–origin lactic acid bacteria, specifically Weissella paramesenteroides, as a promising source for glutathione production
PENANGANAN SAMPAH ORGANIK BERBASIS BIOPORI MELALUI PROGRAM KKN UNIVERSITAS MUHAMMADIYAH BANDUNG DI DESA CIMUJA DUSUN PASANGGRAHAN Azmi Asyfia; Muhammad Salman; Fatimah Azzahra; Hanifa Nida An Khafya; Intan Reynanti; M Yunus; Abdillah Hi Husain; Nisrina Muthia Yumna Fakhrudin; Noval Wijaya; Salma Nuraini Ginanjar; Nelis Hernahadini
Journal of Community Service Vol 7 No 2 (2025): JCS, December 2025
Publisher : Ikatan Dosen Menulis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56670/jcs.v7i2.359

Abstract

Sampah menjadi permasalahan serius masyarakat, dan sampah yang dihasilkan didominasi oleh sampah organik. Di Desa Cimuja Dusun Pasanggrahan, sebagian warga masih membuang sampah ke sungai atau membakarnya di lahan terbuka sehingga menimbulkan pencemaran. Melalui program Kuliah Kerja Nyata (KKN) Universitas Muhammadiyah Bandung, kegiatan ini bertujuan meningkatkan kesadaran masyarakat serta mengimplementasikan teknologi Lubang Resapan Biopori (LRB) sebagai solusi pengelolaan sampah organik. Metode yang digunakan meliputi sosialisasi, diskusi partisipatif, pemutaran media edukatif, serta praktik langsung pembuatan LRB. Hasil kegiatan menunjukkan adanya peningkatan pemahaman warga mengenai dampak sampah organik dan manfaat biopori. Sebagai percontohan, dua lubang resapan berhasil dibuat dengan partisipasi aktif masyarakat. Program ini tidak hanya mengurangi timbulan sampah organik, tetapi juga menghasilkan kompos yang bermanfaat. Dengan demikian, penerapan LRB melalui KKN dapat menjadi model pemberdayaan masyarakat yang ramah lingkungan dan berkelanjutan.
Creation of a Family Medicinal Plant Garden in the Community Service Program in Badama Region, Cimalaka Village, Sumedang Muhammad Panji Abdurrahman; Nesywa Nur Aini; Hanadia Rahmani Gania Putri; Fathiya Reina Maulidia; Mochamad Bintang; Hawa Aufa; Imel Aura Agustin; Habib Khirzin Al Mahdaly; Halimah, Nur; Nopiyanti Rahmawati; Nelis Hernahadini
Journal of Community Service Vol 7 No 2 (2025): JCS, December 2025
Publisher : Ikatan Dosen Menulis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56670/jcs.v7i2.363

Abstract

TOGA (Family Medicinal Plant) is a plant that is efficacious and is used to meet family needs for medicines. This TOGA aims to meet the community's needs for plant-derived drugs, thereby building health as a catalyst for community empowerment activities. The implementation of this service was carried out in Badama hamlet with the Real Work Lecture (KKN) scheme, to increase the community's understanding of the benefits of TOGA. In general, the people of Badama Hamlet work as farmers. In addition, this village has the potential for land development, including the cultivation of medicinal plants. The methods employed in the implementation of the service include socialization, the collection of medicinal plants, and the creation of a TOGA garden. The result of this activity is a TOGA garden that the community can use for medicinal needs.
Co-Authors Abdillah Hi Husain Azmi Asyfia Fathiya Reina Maulidia Fatimah Azzahra Habib Khirzin Al Mahdaly Hanadia Rahmani Gania Putri Hanifa Nida An Khafya Haryanto Haryanto Hawa Aufa Ima Mukaromah Imel Aura Agustin Intan Reynanti Luthfia Hastiani Muharram M Yunus Mochamad Bintang Muhammad Panji Abdurrahman Muhammad Salman Nesywa Nur Aini Nisrina Muthia Yumna Fakhrudin Nopiyanti Rahmawati Noval Wijaya Nur Halimah Salma Nuraini Ginanjar Wulan Pertiwi