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All Journal ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) EnviroScienteae Biosaintifika: Journal of Biology & Biology Education Agrinimal Jurnal Ilmu Ternak dan Tanaman Jurnal Kedokteran Hewan Journal of Animal Research in Wetland Ecosystem
Muhammad Rizal
Department Of Animal Science Faculty Of Agriculture University Of Lambung Mangkurat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Jurnal Veteriner

 1 
Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC) Muhammad Rizal; Muhammad Riyadhi; Bambang Irawan; Anis Wahdi; Habibah Habibah; Herdis Herdis
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.681 KB) | DOI: 10.19087/jveteriner.2017.18.4.571

Abstract

The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC. Five testis with epididymides of crossbreed cattle were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure methods of cauda epididymides with saline solution (0.9% NaCl). Collected-spermatozoa was equally divided in volume into four tubes and diluted with lactose extender containing 20% egg yolk (control), 90% coconut water + 10% egg yolk (CWEY10), 85% coconut water + 15% egg yolk (CWEY15), and 80% coconut water + 20% egg yolk (CWEY20), repectively. Diluted-spermatozoa was stored in refrigerator at 5oC. Quality of dilutedspermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), spermatozoa with intact plasma membrane (IPM) were evaluated every day during four days of storage. Data were analyzed by using completely randomized design with four treatments and five replicates. Means were compared with significant difference test at 0.05 significant level. Results of this study showed that mean of spermatozoa concentration, percentage of MS, percentage of LS, percentage of spermatozoa abnormal, and percentage of IPM of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively. At day-4 of the storage, percentages of MS, LS, and IPM of control (43, 52.2, 59.2%) and CWEY20 (42, 52, 59%) were significantly (P<0.05) higher than CWEY10 (33, 45.4, 52.8%) and CWEY15 (37, 50, 54.6%). In conclusion, lactose and CWEY20 extenders could be maintaining the quality of epidydimal spermatozoa of crossbreed cattle for three days preservation at 5oC and two days for CWEY10 and CWEY15.
Fetal Bovine Serum dalam Pengencer Tris Mempertahankan Kehidupan dan Keutuhan Membran Plasma Spermatozoa Semen Beku Domba Garut (FETAL BOVINE SERUM IN TRIS EXTENDER MAINTAINING SPERMATOZOA VIABILITY AND PLASMA MEMBRANE INTEGRITY OF GARUT RAM FROZEN SEMEN Muhammad Rizal; Herdis .; Insun Sangadji
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.834 KB)

Abstract

The objective of this study was to examine the effectiveness of fetal bovine serum (FBS) against thequality of garut ram frozen semen. Semen was collected from one mature garut ram using artificial vagina.Fresh semen were evaluated then divided into four tubes at equal volume and each tube were diluted withTris extender containing 20% egg yolk (TEY-20, as control); TEY-20 + 8% FBS (FBS-8); TEY-20 + 10% FBS(FBS-10); and TEY-20 + 12% FBS (FBS-12), respectively. Semen at the concentration of 100x106 motilespermatozoa was loaded in 0.25 ml mini straw. Semen was equilibrated at 50C for three hours, then freezeand stored in liquid nitrogen container. The quality of the spermatozoa including percentages of motileand live spermatozoa, intact plasma membrane (IPM) were evaluated following diluting, equilibratingand thawing process. A Complete Randomized design using four treatments and five replicates were usedin the study. The results showed that there was no significant difference (p<0.05) in percentage of motilespermatozoa following thawing between the control (44.0%) and FBS-8 (46.0%), FBS-10 (48.0%), andFBS-12 (47.0%), respectively. The percentage of live spermatozoa and IPM were significantly higher (p<0.05)in the FBS-8 (69.0% and 58.2%); FBS-10 (72.4% and 61.2%); FBS-12 (72.2% and 64.4%) compared to thecontrol (64.8% and 52.8%), respectively. In conclusion, the addition of FBS into Tris extender was able tomaintain the viability and integrity of plasma membrane of garut ram frozen semen.
Kualitas Semen Beku Kambing Peranakan Boer yang Dikriopreservasi dengan Pengencer Tris Kuning Telur dan Berbagai Konsentrasi Ekstrak Daun Kelor Muhammad Rizal; Chatimatun Nisa; Ririn Norliani
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.589 KB) | DOI: 10.19087/jveteriner.2021.22.3.309

Abstract

Daun kelor mengandung berbagai zat nutrien yang dapat melindungi spermatozoa selama kriopreservasi semen. Tujuan penelitian ini adalah untuk menguji pengaruh ekstrak daun kelor di dalam pengencer tris kuning telur terhadap kualitas semen beku kambing peranakan Boer. Semen segar dibagi ke dalam empat buah tabung reaksi dengan volume yang sama, kemudian disentrifugasi dengan kecepatan 3.000 RPM selama 20 menit. Plasma semen dibuang, dan sedimen (spermatozoa) diencerkan dengan empat pengencer berbeda sebagai perlakuan. Penelitian menggunakan rancangan acak lengkap dengan empat perlakuan, yakni: 73% pengencer dasar tris + 20% kuning telur + 7% gliserol tanpa ekstrak daun kelor (kontrol), 71% pengencer dasar tris + 20% kuning telur + 7% gliserol + 2% ekstrak daun kelor (EDK-2), 69% pengencer dasar tris + 20% kuning telur + 7% gliserol + 4% ekstrak daun kelor (EDK-4), dan 67%pengencer dasar tris + 20% kuning telur + 7% gliserol + 6% ekstrak daun kelor (EDK-6). Semen dikemas di dalam straw mini, diekuilibrasi di dalam lemari es pada suhu 5oC selama empat jam dan dibekukan dengan cara meletakkan straw 10 cm di atas permukaan nitrogen cair selama 15 menit. Peubah yang diamati adalah persentase motilitas, persentase hidup, dan persentase membran plasma utuh (MPU) spermatozoa dievaluasi setelah pengenceran dan thawing. Hasil penelitian menunjukkan bahwa penambahan ekstrak daun kelor ke dalam pengencer tris kuning telur dapat mempertahankan kualitas spermatozoa semen beku kambing peranakan boer. Penambahan sebanyak 4% ekstrak daun kelor merupakan konsentrasi terbaik dalam menghasilkan semen beku kambing peranakan boer. Persentase motilitas spermatozoa setelah thawing pada perlakuan kontrol, EDK-2, EDK-4, dan EDK-6 masing-masing adalah 41,25%, 45%, 50%, dan 47,5%. Dapat disimpulkan bahwa penambahan 4% ekstrak daun kelor ke dalam pengencer tris kuning telur merupakan konsentrasi terbaik dalam menghasilkan semen beku kambing peranakan boer.
Fertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkan dengan Pengencer Nira Aren (FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS) DILUTED WITH SUGAR PALM JUICE EXTENDER) Muhammad Rizal; Muhammad Riyadhi
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.187 KB)

Abstract

Sugar palm juice (Arenga pinnata Merr.) can be used as semen extender because its various nutrientscontent which were needed for spermatozoa preservation. The objective of this research was to examineeffectivity of sugar palm juice as swamp buffalo semen extender in artificial insemination (AI) program.Semen of swamp buffalo were collected by using an artificial vagina. Fresh semen were evaluated anddivided in equal volume into four tubes and diluted with lactose extender containing 20% egg yolk (lactoseor control), 85% sugar palm juice + 15% egg yolk (PJEY15), 80% sugar palm juice + 20% egg yolk (PJEY20),and 75% sugar palm juice + 25% egg yolk (PJEY25), respectively. Diluted-semen were preserved inrefrigerator at 5oC, and quality of spermatozoa including percentages of motile spermatozoa, livespermatozoa, and intact plasma membrane (IPM) were evaluated every day for four days. A total of 13female swamp buffaloes were estrous synchronize with PGF2a, and six buffaloes were inseminated withlactose and seven buffaloes were inseminated with PJEY20. Results of this research showed that at dayfourpreservation, mean percentages of motile and live spermatozoa for lactose (36.67 and 51.83%) andPJEY20 (35.83 and 51.5%) were significantly (P<0.05) higher than PJEY15 (31.67 and 44%) and PJEY25 (30 and 45.33%), but no difference significant between treatment for IPM parameter. Pregnancy rate forlactose was 66.67% and 71.43% for PJEY20. Percentage of birth for lactose and PJEY20 were 66.67% and71.43%, respectively. In conclusion, sugar palm juice could be used as an alternative extender in semenpreservation process and AI of swamp buffalo.
Kriopreservasi Semen Domba Garut dengan Pengencer Tris yang Disuplementasi Ethylene Diamine Tetraacetic Acid (CRYOPRESERVATION OF GARUT RAM SEMEN WITH TRIS EXTENDER ETHYLENEDIAMINETETRAACETIC ACID) Muhammad Rizal; Herdis .; Nasrullah .; Muhammad Riyadhi; Insun Sangadji; Yulnawati .
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.369 KB)

Abstract

Ethylene diamine tetraacetic acid (EDTA) is a molecular structure that binds to heavy metals andother toxins which needed to protect the spermatozoa during the cryopreservation process. The objectiveof this research was to examine EDTA in maintain the quality of garut ram frozen semen. Semen werecollected from mature Garut ram by artificial vagina. Fresh semen were evaluated and divided in equalvolume into three tubes and diluted with Tris extender containing 20% egg yolk (TEY-20) as control, TEY-20 + 0.01% EDTA (EDTA0.01), and TEY-20 + 0.02% EDTA (EDTA0.02), respectively. Semen was loaded in 0.25 mL mini straw with the concentration of 100 million motile spermatozoa. Semen was equilibrated at5oC for three hours, then frozen and stored in liquid nitrogen container. Quality of spermatozoa includingpercentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) wereevaluated after diluting, equilibrating, and thawing. Data were analyzed using completely randomizeddesign with three treatments and five replicates. Means were compared significant difference test at 0.05significant level. Results of this research showed that mean volume, color, consistency, pH, mass movement,spermatozoa concentration, percentage of MS, percentage of LS, percentage of abnormal spermatozoa,and percentage of IPM of garut ram fresh semen were 0.87 mL, cream, thick, 6.98, 3, 4,296 million cell/mL,75%, 86.33%, 4.4%, and 85%, respectively. Percentages of MS, LS, and IPM after thawing in control (42.5,52.5, and 52.17%) was not significantly difference with EDTA0.01 (44.17, 52.5, and 52.5%) and EDTA0.02(39.17%, 51%, and 48.33%). In conclusion, the addition of EDTA in Tris extender can maintain quality ofGarut ram frozen semen, and its suitable using in artificial insemination program. Addition of EDTA cannot enhancing quality of garut ram frozen semen.
Co-Authors . Mildawati Abrani Sulaiman Agus Setiawan Akbar Budiansa Al-Azhar Al-Azhar Anis Wahdi Bambang Irawan Chatimatun Nisa Danang Biyatmoko Habibah Habibah Hendra Saputra Herdis . Herdis Herdis Herdis Herdis Herdis Herdis I Wayan Angga Darmawan Insun Sangadji Jodi Aulia Ramadhan Kartini Eriani Lilis Hartati Muhammad Riyadhi Muhammad Riyadhi Muhammad Thahir N. Anita Nasrullah . Nursyam Andi Syarifuddin Ririn Norliani Tati Inderawati Yonadiah Dwitya Yulnawati .