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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Ilmu Ternak JURNAL GALUNG TROPIKA Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pertanian Agros Biosel: Biologi Science and Education Jurnal Hutan Pulau-Pulau Kecil : Jurnal Ilmu-Ilmu Kehutanan dan Pertanian Agrinimal Jurnal Ilmu Ternak dan Tanaman Jurnal Agrosilvopasture-Tech Rumphius Pattimura Biological Journal Maanu : Jurnal Pengabdian Kepada Masyarakat
Insun Sangadji
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics Veterinary

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Perubahan Nilai Nutrisi Ampas Sagu selam pada Fase Pertumbuhan Jamur Tiram Putih (Pleurotus ostreatus) yang berbeda Insun Sangadji; Aminuddin Parakkasi; Komang G. Wiryawan; Budi Haryanto
Jurnal Ilmu Ternak Vol 8, No 1 (2008)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v8i1.2209

Abstract

Tujuan penelitian ini untuk mengetahui  kandungan nutirisi ampas sagu yang dipermentasi menggunakan   Pleurotus ostreatus   pada berbegai  fase pertumbuhannnya.  Rancangan Acak lengkap digunakan pada penelitian ini, perlakuan yang diberikan adalah fase pertumbuhan dari Pleurotus ostreatu. Peubah yang diukur meliputi  kandungan protein kasar, bahan kering, ADF, VDF, dan lignin dari ampas sagu yang difermentasi.  Hasil penelitian menunjukkkan bahwa terdapat perubahan komposisi nutrisi ampas sago pada berbagai fase pertumbuhan Jamur Pleurotus ostreatus. Kandungan protein meningkat dan kandungan ligninoselulosa menurun, sehingga hasil fermentasi ini sangat potensial untuk dijadikan sebagai  pakan ternak ruminansia.Kata kunci: Ampas sago, Pleurotus ostreatus,  pakan ruminansia.
Fetal Bovine Serum dalam Pengencer Tris Mempertahankan Kehidupan dan Keutuhan Membran Plasma Spermatozoa Semen Beku Domba Garut (FETAL BOVINE SERUM IN TRIS EXTENDER MAINTAINING SPERMATOZOA VIABILITY AND PLASMA MEMBRANE INTEGRITY OF GARUT RAM FROZEN SEMEN Muhammad Rizal; Herdis .; Insun Sangadji
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.834 KB)

Abstract

The objective of this study was to examine the effectiveness of fetal bovine serum (FBS) against thequality of garut ram frozen semen. Semen was collected from one mature garut ram using artificial vagina.Fresh semen were evaluated then divided into four tubes at equal volume and each tube were diluted withTris extender containing 20% egg yolk (TEY-20, as control); TEY-20 + 8% FBS (FBS-8); TEY-20 + 10% FBS(FBS-10); and TEY-20 + 12% FBS (FBS-12), respectively. Semen at the concentration of 100x106 motilespermatozoa was loaded in 0.25 ml mini straw. Semen was equilibrated at 50C for three hours, then freezeand stored in liquid nitrogen container. The quality of the spermatozoa including percentages of motileand live spermatozoa, intact plasma membrane (IPM) were evaluated following diluting, equilibratingand thawing process. A Complete Randomized design using four treatments and five replicates were usedin the study. The results showed that there was no significant difference (p<0.05) in percentage of motilespermatozoa following thawing between the control (44.0%) and FBS-8 (46.0%), FBS-10 (48.0%), andFBS-12 (47.0%), respectively. The percentage of live spermatozoa and IPM were significantly higher (p<0.05)in the FBS-8 (69.0% and 58.2%); FBS-10 (72.4% and 61.2%); FBS-12 (72.2% and 64.4%) compared to thecontrol (64.8% and 52.8%), respectively. In conclusion, the addition of FBS into Tris extender was able tomaintain the viability and integrity of plasma membrane of garut ram frozen semen.
Kriopreservasi Semen Domba Garut dengan Pengencer Tris yang Disuplementasi Ethylene Diamine Tetraacetic Acid (CRYOPRESERVATION OF GARUT RAM SEMEN WITH TRIS EXTENDER ETHYLENEDIAMINETETRAACETIC ACID) Muhammad Rizal; Herdis .; Nasrullah .; Muhammad Riyadhi; Insun Sangadji; Yulnawati .
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.369 KB)

Abstract

Ethylene diamine tetraacetic acid (EDTA) is a molecular structure that binds to heavy metals andother toxins which needed to protect the spermatozoa during the cryopreservation process. The objectiveof this research was to examine EDTA in maintain the quality of garut ram frozen semen. Semen werecollected from mature Garut ram by artificial vagina. Fresh semen were evaluated and divided in equalvolume into three tubes and diluted with Tris extender containing 20% egg yolk (TEY-20) as control, TEY-20 + 0.01% EDTA (EDTA0.01), and TEY-20 + 0.02% EDTA (EDTA0.02), respectively. Semen was loaded in 0.25 mL mini straw with the concentration of 100 million motile spermatozoa. Semen was equilibrated at5oC for three hours, then frozen and stored in liquid nitrogen container. Quality of spermatozoa includingpercentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) wereevaluated after diluting, equilibrating, and thawing. Data were analyzed using completely randomizeddesign with three treatments and five replicates. Means were compared significant difference test at 0.05significant level. Results of this research showed that mean volume, color, consistency, pH, mass movement,spermatozoa concentration, percentage of MS, percentage of LS, percentage of abnormal spermatozoa,and percentage of IPM of garut ram fresh semen were 0.87 mL, cream, thick, 6.98, 3, 4,296 million cell/mL,75%, 86.33%, 4.4%, and 85%, respectively. Percentages of MS, LS, and IPM after thawing in control (42.5,52.5, and 52.17%) was not significantly difference with EDTA0.01 (44.17, 52.5, and 52.5%) and EDTA0.02(39.17%, 51%, and 48.33%). In conclusion, the addition of EDTA in Tris extender can maintain quality ofGarut ram frozen semen, and its suitable using in artificial insemination program. Addition of EDTA cannot enhancing quality of garut ram frozen semen.
LAMA PENYIMPANAN DAGING SAPI TERHADAP ALT BAKTERI Insun Sangadji
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol 2, No 1 (2013): BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.395 KB) | DOI: 10.33477/bs.v2i1.139

Abstract

KANDUNGAN ANTOSIANIN DI DALAM MAHKOTA BUNGA BEBERAPA TANAMAN HIAS Insun Sangadji; Muhammad Rijal; Yulia Astri Kusuma
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol 6, No 2 (2017): BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.993 KB) | DOI: 10.33477/bs.v6i2.163

Abstract

FIELD GRASS SILAGE WITH THE ADDITION OF COLI FRUIT FIBER QUESTION AS RUMINANT ANIMAL FEED Saiklela, Desy Megi; Sangadji, Insun; Patty, Christian Willem
RUMPHIUS Vol 5 No 2 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i2p033-037

Abstract

This research aims to find out what % of coli fruit fiber juice is used to produce good quality silage, and to find out the physical quality of field grass silage with the addition of coli fruit fiber juice. The materials used in this research were field grass and coli fruit fiber juice brought from Moa Island, Southwest Maluku Regency. This research will be carried out for 2 months (October-November, 2022) at the Faculty of Agriculture, Animal Husbandry Department, Pattimura University, Ambon. The harvested silage will be subject to observation and/or physical analysis of field grass silage at the Animal Nutrition and Forage Laboratory, Pattimura University, Ambon. The variables observed were color, aroma, texture, pH and the presence or absence of mold in the silage. The physical quality was tested by the 5 students selected as panelists using the questionnaire provided.
Keberlanjutan Usaha Peternakan Domba Kisar dan Strategi Pengembangannya di Pulau Kisar Provinsi Maluku Tatipikalawan, Jomima Martha; Sangadji, Insun
Jurnal Galung Tropika Vol 13 No 2 (2024)
Publisher : Fakultas Pertanian, Peternakan dan Perikanan Universitas Muhammadiyah Parepare

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v13i2.1216

Abstract

This study aims was to analyze the sustainability index and status of the Kisar sheep business and establish a strategy for the sustainability of Kisar sheep development. This study was implemented in Kisar Island, Maluku Province, using 95 Kisar Sheep farms and 15 expert respondents selected by purposive sampling. The data collection methods are literature study, Focus Group Discussion (FGD), in-depth interviews, and surveys. The research variables are 48 sustainable Kisar sheep development attributes from ecological, sociocultural, economic, technology-infrastructure, and legal-institutional dimensions. The data was analyzed using the Rap-DOMKI coordination technique through the Multi-dimensional Scaling (MDS) method to measure the sustainability indexes and status, Leverage analysis, and Monte Carlo analysis. The study results showed that the business was sustainable in ecological, sociocultural, economic, and technological infrastructure dimensions. However, it needed to be more sustainable in the institutional-legal dimension. It was found that 13 attributes sensitively influence business sustainability. Monte Carlo analysis showed that the difference in the sustainability index value of the five sustainability dimensions was very small or less than one (at a confidence level of 95%), which indicates a high precision level. The MDS Rap-DOMKI analysis showed that the stress value was 13-14% and the coefficient determination (R2) of 0.95. It indicates that the MDS Rap-DOMKI analysis model is adequate for estimating the sustainability of Kisar sheep development. It has a high confidence level and can be used as an evaluation tool for fast analysis of sheep development sustainability in one region.
Chemicals Identification in The Ethyl Acetate Fraction and The Antioxidant Activity from Calabash Seed (Cresentia cujete) Extract Gaspersz, Nelson; Kapelle, Imanuel B. D.; Hattu, Nikmans; Sangadji, Insun; Arpipi, Henderika S. S.
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1144-1153

Abstract

Calabash (Crescentia cujete) is a plant that grows in tropical climates and is scattered and easily found in Indonesia. Calabash is also known to contain several secondary metabolite compounds that have the potential as an antioxidant. This research is purposed to identify phytochemical compounds in calabash fruit seed extract, identify compounds based on the result of LC-MS, and know the antioxidant activity of calabash fruit seeds. The samples were extracted using the maceration method, and then phytochemical screening, LC-MS tests, and antioxidant tests were continued using the DPPH method. This research showed that the ethanol extract of calabash seed positive contains saponin, flavonoids, and triterpenoid compounds. Ethyl acetate extract of calabash seed contains flavonoids, alkaloids, and triterpenoids. The n-hexane extracts positively contain tannins, alkaloids, and steroids. The values of antioxidant activity of ethanol, ethyl acetate, and n-hexane extracts were IC50 = 29.7883, 7.219, and 848.712 ppm (µg/ml), respectively. Ethyl acetate extract as the best antioxidant activity was then tested with LC-MS, and results obtained from 26 compounds with the composition of the largest % area is convolvidine (11.277%), coumaroylquinic acid (8.290%) and taxchinin G (8,233%). They showed that calabash fruit seed extract has very strong antioxidant activity, with the best activity being the ethyl acetate extract of calabash fruit seed.
PEMANFAATAN HASIL OLAHAN LIMBAH AIR KELAPA SEBAGAI BAHAN PEREKAT TERHADAP KUALITAS FISIK BISKUIT PAKAN RUMINANSIA Sulaiman, Kitriyani; Sangadji, Insun; Joris, Lily
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 12 No 2 (2024): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2024.12.2.81-87

Abstract

Penelitian bertujuan untuk mengetahui kualitas fisik biskuit pakan ruminansia dengan perekat hasil olahan limbah air kelapa dengan jumlah yang berbeda. Metode yang digunakan adalah metode eksperimen dengan Rancangan Acak Lengkap (RAL) dimana sebanyak 4 perlakuan dicobakan dengan 6 ulangan. Perlakuan yang diberikan adalah P0 (0% air kelapa sebagai perekat, P1(2,5% air kelapa sebagai perekat), P2 (5% air kelapa sebagai perekat), dan P3 (7,5% air kelapa sebagai perekat). Variabel yang diamati meliputi: warna, tekstur, kerapatan, ketahanan benturan, dan daya serap air biskuit pakan. Hasil penelitian menunjukan hasil olahan limbah air kelapa yang digunakan sebagai bahan perekat menyebabkan biskuit pakan ternak mempunyai kualitas warna skor 1,0 – 2,0, tektur skor 2,0-5,0, kerapatan 0,26 g/cm3, ketahanan benturan 0,04-0,05%, dan daya serap air 2,39-2,72%. Hasil analisis statistik menyimpulkan penggunaan hasil olahan limbah air kelapa sebanyak 7,5% (P3) menunjukkan kualitas fisik biskuit pakan ruminansia yang terbaik pada warna, tekstur dan daya serap air dibanding perlakuan lainnya (p<0,05). ABSTRACT The research aims to determine the physical quality of ruminant feed biscuits with adhesives made from coconut water waste in different quantities. The method used was an experimental method with a Completely Randomized Design (CRD) where 4 treatments were tried with 6 replications. The treatments given were P0 (0% coconut water as adhesive), P1 (2.5% coconut water as adhesive), P2 (5% coconut water as adhesive), and P3 (7.5% coconut water as adhesive). Variables observed included: color, texture, density, impact resistance, and water absorption capacity of feed biscuits. The results of the research showed that the processed coconut water waste used as an adhesive caused animal feed biscuits to have a color quality score of 1.0 - 2.0 and a texture score of 1.0 - 2.0. 2.0-5.0, density 0.26 g/cm3, impact resistance 0.04-0.05%, and water absorption capacity 2.39-2.72%. The results of statistical analysis conclude the use of processed coconut water waste of as much as 7.5% (P3) showed the best physical quality of ruminant feed biscuits in terms of color, texture, and water absorption compared to other treatments (p<0.05).
POTENSI SOSIAL EKONOMI DAN PERAN PETERNAKAN SAPI DALAM MENINGKATKAN PENDAPATAN KELUARGA DI KABUPATEN BURU PROVINSI MALUKU Tatipikalawan, Jomima M; Sangadji, Insun; Ririmase, Pieter M.
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 10 No 1 (2022): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2022.10.1.29-37

Abstract

ABSTRAK Tujuan penelitian ini mengkaji potensi sosial ekonomi peternak sapi, kontribusi usaha peternak sapi terhadap pendapatan keluarga dan faktor-faktor yang mempengaruhi kontribusi pendapatan. Materi penelitian adalah 70 peternak peternak sapi poting pada 2 kecamatan di Kabupaten Buru yang diambil secara purposive sampling. Data yang digunakan adalah data primer dan diambil dengan metode survey melalui wawancara mendalam (in-depth interview) kepada peternakan menggunakan kuisioner. Analisis data secara deskriptif, perhitungan ekonomi, kontribusi pendapatan dan analisis regresi untuk melihat faktor-faktor yang mempengaruhi kontribusi pendapatan usaha sapi potong. Hasil penelitian menunjukkan bahwa potensi yang dimiliki peternak sapi potong di Kabupaten Buru berusia produktif, tingkat pendidikan masuk kategori baik dari menengah sampai atas, memiliki motif ekonomi dengan tujuan sebagai sumber pedapatan dan tabungan. Rataan jumlah kepemilikan 4-6 UT/peternak, dipelihara secara tradisional dengan lama pemeliharaan >4 tahun. Kontribusi pendapatan 49,89% (cabang usaha). Hasil analisis regresi linier berganda menunjukkan kontribusi usaha ternak sapi secara sangat signifikan (P≤0,01) dipengaruhi oleh kepemilikan sapi, pendapatan lainnya (P≤0,05) dan lama usaha (P≤0,1). Peternak dengan lama usaha yang semakin besar cenderung memiliki skala usaha yang lebih besar yang selanjutnya mempengaruhi jumlah sapi yang dipasarkan. Peternak memiliki motif ekonomi yang kuat sehingga usaha peternakan sapi dan pertanian dijalankan dengan optimal untuk mendatangkan pendapatan. ABSTRACT The purpose of this study is to examine the socio-economic potential of cattle breeders, the contribution of cattle farmers' businesses to family income, and the factors that influence the contribution of income. The research material is 70 farmers of beef cattle breeders in 2 Districts in Buru Regency taken by purposive sampling. The data used are primary and taken by survey method through in-depth interviews with farmers using questionnaires. Descriptive data analysis, economic calculations, income contribution, and regression analysis to see the factors that affect the contribution of beef cattle business income. The results show that the potential of beef cattle breeders in Buru Regency is productive age, education level is in the middle to the upper category, has an economic motive, and the purpose of maintenance as a source of income and savings. The average number of ownership is 4-6 au/farmers, traditionally maintained with a maintenance period of >4 years. Revenue contribution 49.89% (business branch). The results of multiple linear regression analysis showed that the contribution of cattle business was very significantly (P≤0.01) influenced by cattle ownership, other income (P≤0.05), and length of business (P≤0.1). Farmers with a longer length of business tend to have a larger scale of business which in turn affects the number of cattle marketed. Farmers have an economic motive so that farming is carried out optimally to bring in income.
Co-Authors Aminuddin Parakkasi Arpipi, Henderika S. S. Gaspersz, Nelson Hattu, Nikmans Herdis . Joris, Lily Kapelle, Imanuel B. D. Kayadoe, Ferens Kilmanun, Nicodemus Gidion Komang G. Wiryawan Matatula, Michel Muhammad Rijal Muhammad Rivai Muhammad Riyadhi Muhammad Rizal Nasrullah . Patty, Christian W Patty, Christian W. Patty, Christian Willem Ralahalu, Tabita Naomi Ririmase, Pieter M. Ririmasse, Pieter Melianus Saiklela, Desy Megi Sarfan, Riri Sitorus, Jojor Desi Sulaiman, Kitriyani Tatipikalawan, Jomima M Tatipikalawan, Jomima Martha Tomatala, George Semuel Johny Tulalessy, Abraham Yulia Astri Kusuma Yulnawati .