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All Journal ACTA VETERINARIA INDONESIANA Buletin Peternakan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) EnviroScienteae Biosaintifika: Journal of Biology & Biology Education Agrinimal Jurnal Ilmu Ternak dan Tanaman Jurnal Kedokteran Hewan Journal of Animal Research in Wetland Ecosystem
Muhammad Rizal
Department Of Animal Science Faculty Of Agriculture University Of Lambung Mangkurat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Viabilitas Spermatozoa Cauda Epididimis Kerbau Rawa dalam Berbagai Konsentrasi Pengencer Air Kelapa Muda dan Kuning Telur Muhammad Riyadhi; Muhammad Rizal; . Mildawati
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.689 KB) | DOI: 10.29244/avi.6.1.38-43

Abstract

Penelitian bertujuan untuk mengetahui viabilitas spermatozoa cauda epididimis pada berbagai konsentrasi pengenceran menggunakan air kelapa muda dan kuning telur yang disimpan pada  temperatur 3-5oC.  Pengencer Tris 80% + 20% kuning telur digunakan sebagai kontrol (P1), selanjutnya perlakuan menggunakan pengencer air kelapa muda 90% + 10% kuning telur (P2), air kelapa muda 85% + 15% kuning telur (P3) dan air kelapa muda 80% + 20% kuning telur (P4). Viabilitas  spermatozoa yang diamati setiap hari meliputi persentase motilitas dan persentase hidup sampai mencapai motilitas minimum 30%. Hasil penelitian menunjukkan persentase motilitas tertinggi pada hari ke-4 terdapat pada perlakuan P4 sebesar 31.8 %, berbeda nyata (P<0.05) dengan P3 sebesar 10.5 % dan P2 sebesar 2.5 % serta dengan P1  sebesar 4.2 %.  Pada persentase hidup spermatozoa, perlakuan P4 juga menunjukan nilai 50.3 %, berbeda sangat nyata (P<0.05) dengan perlakuan P1, P2 serta P3 berturut-turut adalah 27.8 %, 26.4 %, dan 32.9 %.  Kesimpulan dari penelitian menunjukkan bahwa pengenceran dengan perlakuan P4 (80% air kelapa dan 20% kuning telur) mampu mempertahankan viabilitas spermatozoa cauda epididimis kerbau rawa selama proses penyimpanan hingga empat hari di dalam refrigerator dengan suhu 3–5oC.
KUALITAS SPERMATOZOA ASAL CAPUT, CORPUS, DAN CAUDA EPIDIDIMIS PADA KERBAU RAWA (Bubalus bubalis carabenensis) Muhammad Riyadhi; Akbar Budiansa; Muhammad Rizal
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 3, No 3 (2016): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (63.224 KB) | DOI: 10.33772/jitro.v3i3.2575

Abstract

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis).  Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis.  Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality.  Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.
DAYA HIDUP SPERMATOZOA KAMBING PERANAKAN ETAWA YANG DIPRESERVASI DENGAN BERBAGAI JENIS PENGENCER Muhammad Rizal; Muhammad Thahir
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 3, No 3 (2016): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.033 KB) | DOI: 10.33772/jitro.v3i3.2572

Abstract

The purpose of this research was to examine the effectivity of Tris, sodium citrate, and lactose extenders in maintaining viability of etawa crossbreed (EC) goat spermatozoa preserved at 3–5oC.  Semen was collected using artificial vagina once a week for six times as replicate.  Fresh semen was divided into three tubes then diluted with Tris, sodium citrate, and lactose extenders, respectively.  Diluted-semen were stored in refrigerator at 3–5oC.  Quality of diluted-semen including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3–5oC for four days.  Data were analyzed using completely randomized design with three treatments and six replicates.  Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, and spermatozoa abnormal of PE goat fresh semen were 1.05 ml, cream, thick, 6.9, ++ – +++, 3,756.67 million cell/ml, 76.67%, 85.83%, and 4.5%, respectively.  Tris, sodium citrate, and lactose extenders have same ability in maintaining viability of EC goat spermatozoa during preserved at 3–5oC.  At day-4 storage, percentages of MS and LS for Tris (44.17 and 57.83%) was no difference significant with sodium citrate (43.33 and 56.67%), and lactose (43.33 and 54.17%).   In conclusion, Tris, sodium citrate, and lactose extenders have same ability in maintaining viability of EC goat spermatozoa during preserved at 3–5oC.  Tris, sodium citrate, and lactose extenders could be maintaining 40% in percentage of motile spermatozoa for three days after preserved at 3–5oC, and suitable for use in artificial insemination (AI) programKeywords: Tris, sodium citrate, lactose, spermatozoa viability, PE goat.
The Quality of Boer Goat Semen Preserved with Sugar Palm Juice Muhammad Rizal; Muhammad Riyadhi; Abrani Sulaiman
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.28236

Abstract

The objective of this study was to examine the effect of seminal plasma on viability of Boer goat spermatozoa and effectiveness of sugar palm juice as an alternative extender during preservation at 5ºC. Semen of two Boer goats were collected using an artificial vagina. Fresh semen were evaluated and divided in equal volume into four tubes. Semen in the first and second tubes diluted with 80% sugar palm juice + 20% egg yolk (P1) and Andromed (P2), respectively. Semen in the third and fourth tubes were centrifuged with 3,000 RPM for 20 minutes, and the supernatant removed. diluted with 80% sugar palm juice + 20% egg yolk (P3) and Andromed (P4), respectively. Diluted-semen were preserved in refrigerator at 5oC, and quality of the spermatozoa including motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated every day for four days. Results of this study showed that at day-2 preservation, mean percentages of MS, LS, and IPM for P2 (72, 83.4, and 83.4%), P3 (72, 82.6, and 82.2%), and P4 (72, 83, 83.8%) were significantly (P<0.05) higher than P1 (3, 24.8, and 25.2%). At day-3 preservation, mean percentages of MS, LS, and IPM for P2 (57, 65.6, and 69.6%) was significantly (P<0.05) higher than P3 (21, 34.8, and 31.8%), P4 (22, 33.6, and 31.2%), and P1 (0, 0, and 0%). In conclusion, semen of Boer goat to be preserved with extender containing egg yolk should be removed seminal plasma. Sugar palm juice containing egg yolk could be used as an extender for Boer goat semen, but should be applied in the AI program immediately after the semen is diluted.
PERSEPSI DAN PARTISIPASI MASYARAKAT TERHADAP PROGRAM PENGEMBANGAN BIOGAS DI KECAMATAN TAMBAN CATUR KABUPATEN KAPUAS Tati Inderawati; Danang Biyatmoko; Muhammad Rizal; Lilis Hartati
EnviroScienteae Vol 12, No 3 (2016): Enviroscienteae Volume 12 Nomor 3, November 2016
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.979 KB) | DOI: 10.20527/es.v12i3.2453

Abstract

Biogas is a renewable energy source needed as an alternative potential fuel.  This research aims to 1) analyse perceptions and participations of community to the development of biogas in Kecamatan Tamban Catur, Kabupaten Kapuas, 2) identify factors influencing the success of biogas development in Kecamatan Tamban Catur, Kabupaten Kapuas, and 3) analyse the benefits of using biogas for community in Kecamatan Tamban Catur, Kabupaten Kapuas.  The research was carried out in Sidomulyo, Sidorejo, and Warnasari Villages in Kecamatan Tamban Catur, Kapuas Regency. The research was conducted using survey data, and the data were further analyzed through descriptive statistics involving 33 respondents who have received the program of biogas development in Kecamatan Tamban Catur Kapuas Regency.  The dependent variables were perceptions and participations of community while the independent variables were ages, the number of cattle, education, supporting program, and duration of biogas utilization. The results indicated that community perceptions of the biogas development were positively increasing (78.8%). The F-Test showed that independent variables influencing community perceptions significantly (P<0.05) with 35.1% of coefficient determination (R2).  Although community participations to biogas development were relatively high (72.7%), there was no apparent impact (P>0.05) of independent variables to the community participations. Factors influencing the success of biogas development in Kecamatan Tamban Catur were passion, the number of cattle, floor types, applicable technology, and supporting programs from the government agencies.  There are several advantages of using biogas for the community. First, the use of biogas improves cost efficiency per month by reducing the usage of fuel/kerosene for about 15 liters (Rp.150.000,-) and the usage of 60 bundles of fire-wood (Rp.120.000,-).  Moreover, utilizing solid and liquid organic fertilizer increased daily income for Rp. 10.000,- and Rp. 48.000,- respectively.  In addition, biogas application is more environmentally-friendly.
PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) Herdis Herdis; I Wayan Angga Darmawan; Muhammad Rizal
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.204 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5139

Abstract

ABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram.
EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS Muhammad Riyadhi; Agus Setiawan; Herdis Herdis; Muhammad Rizal
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.168 KB) | DOI: 10.21157/j.ked.hewan.v11i1.5136

Abstract

This research was conducted to investigate the effect of various concentrations of lactose supplementation in Tris extender for maintaining the quality of Etawa crossbreed goat epididymal spermatozoa stored at 3-5° C. Semen in the control group was diluted with a tris extender containing 20% egg yolk without lactose. Semen in the test groups was diluted with a tris extender containing 20% egg yolk and added with 0.3% (0.3 g per 100 mL extender) and 0.6% lactose for group TL1 and TL2, respectively. Parameters evaluated of the fresh epididymal spermatozoa were motility, concentration, percentage of live, and abnormality of spermatozoa, while for diluted-spermatozoa were motility and percentage of live spermatozoa. Spermatozoa observation was conducted until it reaches 40% motility. The results showed that the mean percentage of motility, live sperm, concentration, and abnormality of epididymal spermatozoa were 70%; 81%; 3,220x106 cells/mL; and 4.30%, respectively in all group. After dilution, the percentage of motility and live spermatozoa were also 70% and 81.00±1.58%, respectively in all groups. The decreasing of spermatozoa motility was observed on day 4 of storage, in which percentage of spermatozoa motility in control group (40.00±0.00%) was significantly lower (P0.05) than those in TL1 (44.00±2.24%) and TL2(45.00±0.00%) groups. Percentage of live spermatozoa in control (63.20±2.68%) was not significantly different (P0.05) than TL1(65.40±1.95%) and TL2 (65.60±1.95%). In conclusion, the supplementation of lactose into Tris extender could maintain the epididymal spermatozoa of Etawa crossbreed for 3 days of storage at 3-5° C.
Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation Yonadiah Dwitya; Kartini Eriani; Hendra Saputra; Al-Azhar Al-Azhar; Muhammad Rizal
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18033

Abstract

Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.
DAYA HIDUP SPERMATOZOA KAMBING PERANAKAN ETAWAH YANG DIPRESERVASI DENGAN PENGENCER AIR TEBU Jodi Aulia Ramadhan; Muhammad Riyadhi; Nursyam Andi Syarifuddin; Anis Wahdi; Muhammad Rizal
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 11 No 1 (2023): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2023.11.1.45-50

Abstract

Air tebu dapat dimanfaatkan sebagai pengencer semen karena mengandung berbagai nutrien yang dapat menunjang kehidupan spermatozoa selama preservasi. Penelitian ini bertujuan untuk menguji kemampuan air tebu dalam mempertahankan daya hidup spermatozoa kambing PE selama preservasi pada suhu 3-5oC. Semen ditampung menggunakan vagina buatan dan segera dievaluasi secara makroskopis dan mikroskopis. Semen segar dibagi ke dalam lima buah tabung reaksi kemudain masing-masing diencerkan dengan 80% pengencer dasar laktosa + 20% kuning telur sebagai kontrol (P0), 20% air tebu + 60% akuabidetislata + 20% kuning telur (P1), 30% air tebu + 50% akuabidestilata + 20% kuning telur (P2), 40% air tebu + 40% akuabidestilata + 20% kuning telur (P3), dan 50% air tebu + 30% akuabidestilata + 20% kuning telur (P4). Semen yang telah diencerkan disimpan di dalam refrigerator lemari es pada suhu 3-5oC. Motilitas dan daya hidup spermatozoa masing-masing perlakuan dievaluasi setiap hari hingga motilitas mencapai 40%. Hasil penelitian diperoleh karakteristik semen segar kambing PE yaitu volume 1,15 ml, konsentrasi 3.870 juta/ml, motilitas 80%, spermatozoa hidup 87,75%, dan spermatozoa abnormal 7,5%. Persentase motilitas dan spermatozoa hidup pada hari keempat preservasi perlakuan P2 (41,25% dan 50%) nyata (P<0,05) lebih tinggi dibandingkan dengan P0 (37,5% dan 47%), P1 (32,5% dan 44%), P3 (30% dan 36,25%), dan P4 (18% dan 29,25%). Dapat disimpulkan bahwa Kombinasi pengencer terbaik untuk semen kambing PE adalah terdiri atas 30% air tebu + 50% akuabidestilata + 20% kuning telur. ABSTRACT Sugarcane juice can be used as an alternative semen extender because it contains various nutrients that can support the life of spermatozoa during preservation. This study aimed to examine the ability of sugarcane juice to maintain the ability of Etawah crossbreed goat spermatozoa during preservation at 3-5oC. Semen was collected using an artificial vagina and immediately evaluated macroscopically and microscopically. Fresh semen was divided into five tubes and diluted with 80% lactose-based extender + 20% egg yolk as a control (T0), 20% sugarcane juice + 60% aquabidestilata + 20% egg yolk (T1), 30% sugarcane juice + 50% aquabidestilata + 20% egg yolk (T2), 40% sugarcane juice + 40% aquabidestilata + 20% egg yolk (T3), and 50% sugarcane juice + 30% aquabidestilata + 20% egg yolk (T4), respectively. The diluted-semen was stored in the refrigerator at 3-5oC. The motility and viability of spermatozoa were evaluated every day until the motility reached 40%. The results showed that the characteristics of fresh semen of Etawah crossbreed goat were volume 1.15 ml volume, spermatozoa concentration 3870 million cells/ml, spermatozoa motility 80%, live spermatozoa 87.75%, and abnormal spermatozoa 7.5%. The percentage of motility and live spermatozoa on the fourth day of preservation for T2 (41.25% and 50%) was significantly (P<0.05) higher than T0 (37.5% and 47%), T1 (32.5% and 44%), T3 (30% and 36.25%), and T4 (18% and 29.25%). It can be concluded that the best extender combination for Etawah crossbreed goat semen is composed of 30% sugarcane juice + 50% aquabidestilata + 20% egg yolk.
Pemanfaatan Tepung Kulit Pisang Sebagai Bahan Pakan Alternatif Terhadap Kualitas Karkas Itik Raja N. Anita; Abrani Sulaiman; Muhammad Rizal
JURNAL PENELITIAN PETERNAKAN LAHAN BASAH Vol 2 No 1 (2015)
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpplb.v2i1.1788

Abstract

Penelitian bertujuan untuk menguji pengaruh penggunaan tepung kulit pisang (TKP) sebagai bahanpakan alternatif pada ransum terhadap kualitas karkas yang meliputi: bobot potong, persentasekarkas, presentase giblet dan persentase lemak abdominal itik Raja. Metode yang digunakan adalahrancangan acak lengkap (RAL) dengan empat perlakuan dan lima kali ulangan. Jumlah itik Rajadalam penelitian ini adalah 60 ekor. Sebagai perlakuan dalam percobaan adalah KP0 (Tanpapenggunaan TKP dalam ransum), KP1 (penambahan 12% TKP dalam ransum), KP2 (penambahan24% TKP dalam ransum), dan KP3 (penambahan 36% TKP dalam ransum). Pakan yang diberikansesuai rekomendasi NRC. Pakan dan air minum diberikan ad libitum selama 42 hari percobaan.Data dianalisis menggunakan analisis ragam, dan jika menunjukkan pengaruh yang nyatadilanjutkan dengan uji beda nilai tengah menggunakan uji beda nyata terkecil. Hasil percobaanmenunjukkan bahwa penggunaan TKP pisang berpengaruh sangat nyata (P<0,01) terhadap bobotpotong, tidak berpengaruh nyata (P>0,05) terhadap persentase karkas, serta berpengaruh nyata(P<0,05) terhadap persentase giblet dan persentase lemak abdominal itik raja. Disimpulkan bahwapenggunaan TKP dapat menjadi bahan pakan alternatif dengan batasan penggunaan 10%-24%dalam ransum.
Co-Authors . Mildawati Abrani Sulaiman Agus Setiawan Akbar Budiansa Al-Azhar Al-Azhar Anis Wahdi Bambang Irawan Chatimatun Nisa Danang Biyatmoko Habibah Habibah Hendra Saputra Herdis . Herdis Herdis Herdis Herdis Herdis Herdis I Wayan Angga Darmawan Insun Sangadji Jodi Aulia Ramadhan Kartini Eriani Lilis Hartati Muhammad Riyadhi Muhammad Riyadhi Muhammad Thahir N. Anita Nasrullah . Nursyam Andi Syarifuddin Ririn Norliani Tati Inderawati Yonadiah Dwitya Yulnawati .