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All Journal Saintifik : Jurnal Matematika, Sains, dan Pembelajarannya Current Research on Biosciences and Biotechnology JUSTER: Jurnal Sains dan Terapan JPS (Jurnal Pharma Saintika)
Salam, Diah Astari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Other

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Potensi Ekstrak Etanol Bunga Kecombrang (Etlingera Elatior (Jack) R.M. Smith) Sebagai Inhibitor Enzim Tirosinase Muslihin, Nisrina; Salam, Diah Astari
JUSTER : Jurnal Sains dan Terapan Vol. 4 No. 2 (2025): JUSTER: Jurnal Sains dan Terapan
Publisher : Jompa Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57218/juster.v4i2.1779

Abstract

Bunga kecombrang (Etlingera elatior (Jack) R.M. Smith) merupakan bagian tanaman yang diketahui mengandung beragam senyawa bioaktif, seperti flavonoid yang memiliki potensi dalam berbagai aktivitas farmakologis, termasuk sebagai agen pencerah kulit. Salah satu mekanisme yang berperan dalam efek pencerah kulit adalah penghambatan enzim tirosinase, enzim penting dalam jalur pembentukan melanin. Aktivitas enzim tirosinase yang meningkat dapat merangsang produksi melanin berlebih, yang berkontribusi terhadap timbulnya hiperpigmentasi. Penelitian ini bertujuan untuk mengetahui potensi ekstrak etanol bunga kecombrang sebagai penghambat enzim tirosinase. Ekstrak diperoleh melalui metode maserasi dengan pelarut etanol 96%, kemudian diuji secara kualitatif dengan teknik kromatografi lapis tipis (KLT) bioautografi untuk melihat aktivitas penghambatan terhadap enzim tirosinase. Hasil uji KLT bioautografi menunjukkan adanya zona terang pada plat kromatografi, yang mengindikasikan kemampuan ekstrak dalam menghambat aktivitas enzim tirosinase. Temuan ini mendukung pemanfaatan bunga kecombrang sebagai bahan alami potensial dalam pengembangan produk kosmetik pencerah kulit berbasis herbal.
Profil Fitokimia dan Aktivitas Antimikroba Ekstrak Daun Leucas Lavandulifolia Terhadap Mikroba Patogen Feriadi, Eva; Mail, Silvana; Syahruddin, Muh.; Salam, Diah Astari; Muslihin, Nisrina; Agusriyadin, Agusriyadin
SAINTIFIK Vol 11 No 2 (2025): Saintifik: Jurnal Matematika, Sains, dan Pembelajarannya
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/saintifik.v11i2.602

Abstract

Lenglengan (Leucas lavandulifolia) merupakan tanaman herbal dikotil yang tingginya mencapai 0,95 meter dan telah lama digunakan dalam pengobatan tradisional untuk berbagai penyakit, seperti luka, diabetes, demam, batuk, infeksi cacing dan bakteri, luka busuk, dan penyakit mikosis. Penelitian ini bertujuan mengevaluasi potensi antimikroba dari metabolit sekunder pada ekstrak etanol daun L. lavandulifolia terhadap bakteri Escherichia coli dan jamur Candida albicans, serta menentukan profil fitokimia ekstrak tersebut. Uji aktivitas antimikroba menggunakan metode difusi cakram. Hasil penelitian menunjukkan bahwa ekstrak etanol daun L. lavandulifolia mengandung senyawa bioaktif seperti alkaloid, flavonoid, terpenoid, saponin, polifenol, dan kuinon serta mampu menghambat pertumbuhan E. coli dengan daya hambat pada konsentrasi 100 µg/disc adalah 16,1 mm (kuat), pada konsentrasi 50 µg/disc sebesar 13,6 mm (kuat), dan pada konsentrasi 25 µg/disc sebesar 12,9 mm (kuat), sedangkan kontrol positif menunjukkan zona hambat sebesar 43,0 mm. Pada C. albicans, daya hambat pada konsentrasi 100 µg/disc sebesar 14,9 mm (kuat), pada 50 µg/disc sebesar 12,6 mm (kuat), dan pada 25 µg/disc sebesar 8,1 mm (sedang), dengan kontrol positif menunjukkan zona hambat 18,6 mm. Kesimpulan dalam penelitian ini menunjukkan bahwa ekstrak etanol daun L. lavandulifolia ini menunjukkan aktivitas antimikroba yang signifikan terhadap E. coli dan C. albicans.
Liquid chromatography-mass spectrometric analysis of some bioactive compounds in commercial herbal products derived from Moringa oleifera L. Amrianto; Salsabila, Syefira; Salam, Diah Astari
Current Research on Biosciences and Biotechnology Vol. 7 No. 1 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.7.1/XR8PKR5P

Abstract

Moringa oleifera is widely recognized as a “miracle tree” due to its nutritional, medicinal, and environmental benefits. Its diverse phytochemical content underlies significant pharmacological effects. However, conventional HPLC methods for analyzing multi-component compounds in M. oleifera are often time-consuming and lack sensitivity, particularly for compounds present at low concentrations. To address this, we developed and validated an analytical method using UPLC-ESI-MRM/MS combined with ultrasound-assisted extraction (UAE). Method validation covered linearity, sensitivity (LOD, LOQ), accuracy, and precision, while UAE was optimized using Response Surface Methodology (RSM) with Box-Behnken Design. Seven phytochemical compounds—rutin, hyperoside, nicotiflorin, astragalin, niazirin, quercetin, and kaempferol—were quantified in various M. oleifera products including dried powder, herbal tea, extracts, and capsules. The validated method showed linearity between 1.56–500 µg/mL, LOD values of 0.036–0.094 µg/mL, LOQ values of 0.0108–0.2850 µg/mL, with accuracy and precision within ±15%. Optimal extraction was achieved at a solvent ratio of 1:30, temperature of 40 °C, and extraction time of 17.85 min. UPLC-ESI-MRM/MS enabled rapid separation and detection of all seven compounds within 15 minutes, significantly improving resolution compared to UV-based HPLC. In addition, three abundant flavonoids were confirmed as the main flavonoids present in Moringa, mainly rutin, hyperoside and nicotiflorin. On the other hand, niazirin can be considered as the specific marker for Moringa leaves. These results demonstrate that UPLC-ESI-MRM/MS, coupled with optimized UAE, provides a sensitive, rapid, and reliable approach for profiling M. oleifera phytochemicals, supporting its quality assessment and potential applications in nutraceutical and pharmaceutical industries.
SKRINING FITOKIMIA, AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS AKUT INFUSA BIJI DAN PERIKARP BUAH OLAE (Etlingera calophrys) Salam, Diah Astari; Sabandar, Carla Wulandari; Ummul Fausiah; Harni Sartika Kamaruddin; Megawati; Nisrina Muslihin
Journal Pharma Saintika Vol. 9 No. 1 (2025): Oktober : Jurnal Pharma Saintika
Publisher : Program Studi DIII Farmasi Akademi Farmasi Dwi Farma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51225/jps.v9i1.94

Abstract

Olae (Etlingera calophrys), an endemic of Southeast Sulawesi, Indonesia, is traditionally used as a culinary spice and  medicine, yet remains scientifically underexplored. This study investigated the phytochemical composition, antioxidant activity, and acute toxicity of infusion extract from the seeds and fruit pericarp. Phytochemical screening was performed using specific color reagents, antioxidant activity was evaluated with the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method, and acute toxicity was assessed by the Brine Shrimp Lethality Test (BSLT) against Artemia salina. The seed infusion contained alkaloids and phenolics, while the pericarp infusion contained tannins and phenolics. Antioxidant testing revealed very strong radical scavenging activity, with SC₅₀ values of 0.05 g/100 mL (seeds) and 0.04 g/100 mL (pericarp). Toxicity testing indicated no significant toxicity, with LC₅₀ values of 1740 g/100 mL (seeds) and 2510 g/100 mL (pericarp). These findings suggest that infusion extract of E. calophrys possess strong antioxidant potential and are safe for use, supporting their prospective application as natural antioxidant sources.
Co-Authors Agusriyadin, Agusriyadin Amrianto Carla Wulandari Sabandar Eva Feriadi Harni Sartika Kamaruddin Mail, Silvana Megawati Muslihin, Nisrina Nisrina Muslihin Salsabila, Syefira Syahruddin, Muh. Ummul Fausiah